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-基本情報
登録情報 | データベース: PDB / ID: 7qvx | |||||||||
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タイトル | Cryo-EM structure of coxsackievirus A6 altered particle | |||||||||
要素 |
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キーワード | VIRUS / enterovirus / coxsackievirus A6 / altered particle / capsid / cryo-EM | |||||||||
機能・相同性 | 機能・相同性情報 symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MDA-5 activity / picornain 2A / symbiont-mediated suppression of host mRNA export from nucleus / symbiont genome entry into host cell via pore formation in plasma membrane / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / cytoplasmic vesicle membrane / endocytosis involved in viral entry into host cell / channel activity ...symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MDA-5 activity / picornain 2A / symbiont-mediated suppression of host mRNA export from nucleus / symbiont genome entry into host cell via pore formation in plasma membrane / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / cytoplasmic vesicle membrane / endocytosis involved in viral entry into host cell / channel activity / nucleoside-triphosphate phosphatase / monoatomic ion transmembrane transport / RNA helicase activity / induction by virus of host autophagy / RNA-directed RNA polymerase / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / DNA-templated transcription / host cell nucleus / virion attachment to host cell / structural molecule activity / ATP hydrolysis activity / proteolysis / RNA binding / ATP binding / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | Coxsackievirus A6 (コクサッキーウイルス) | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.5 Å | |||||||||
データ登録者 | Buttner, C.R. / Spurny, R. / Fuzik, T. / Plevka, P. | |||||||||
資金援助 | チェコ, 2件
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引用 | ジャーナル: Commun Biol / 年: 2022 タイトル: Cryo-electron microscopy and image classification reveal the existence and structure of the coxsackievirus A6 virion. 著者: Carina R Büttner / Radovan Spurný / Tibor Füzik / Pavel Plevka / 要旨: Coxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous ...Coxsackievirus A6 (CV-A6) has recently overtaken enterovirus A71 and CV-A16 as the primary causative agent of hand, foot, and mouth disease worldwide. Virions of CV-A6 were not identified in previous structural studies, and it was speculated that the virus is unique among enteroviruses in using altered particles with expanded capsids to infect cells. In contrast, the virions of other enteroviruses are required for infection. Here we used cryo-electron microscopy (cryo-EM) to determine the structures of the CV-A6 virion, altered particle, and empty capsid. We show that the CV-A6 virion has features characteristic of virions of other enteroviruses, including a compact capsid, VP4 attached to the inner capsid surface, and fatty acid-like molecules occupying the hydrophobic pockets in VP1 subunits. Furthermore, we found that in a purified sample of CV-A6, the ratio of infectious units to virions is 1 to 500. Therefore, it is likely that virions of CV-A6 initiate infection, like those of other enteroviruses. Our results provide evidence that future vaccines against CV-A6 should target its virions instead of the antigenically distinct altered particles. Furthermore, the structure of the virion provides the basis for the rational development of capsid-binding inhibitors that block the genome release of CV-A6. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7qvx.cif.gz | 144.7 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7qvx.ent.gz | 109.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 7qvx.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 7qvx_validation.pdf.gz | 1.5 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 7qvx_full_validation.pdf.gz | 1.5 MB | 表示 | |
XML形式データ | 7qvx_validation.xml.gz | 41.9 KB | 表示 | |
CIF形式データ | 7qvx_validation.cif.gz | 60.1 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/qv/7qvx ftp://data.pdbj.org/pub/pdb/validation_reports/qv/7qvx | HTTPS FTP |
-関連構造データ
関連構造データ | 14183MC 7qvyC 7qw9C M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
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非結晶学的対称性 (NCS) | NCS oper:
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