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-基本情報
登録情報 | データベース: PDB / ID: 7qet | |||||||||
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タイトル | human Connexin 26 dodecamer at 20mmHg PCO2, pH7.4 | |||||||||
要素 | Gap junction beta-2 protein | |||||||||
キーワード | MEMBRANE PROTEIN / Gap junction / Ion Channel / carbon dioxide sensitive | |||||||||
機能・相同性 | 機能・相同性情報 Transport of connexons to the plasma membrane / response to human chorionic gonadotropin / gap junction-mediated intercellular transport / gap junction channel activity involved in cell communication by electrical coupling / epididymis development / Oligomerization of connexins into connexons / Transport of connexins along the secretory pathway / gap junction assembly / connexin complex / gap junction ...Transport of connexons to the plasma membrane / response to human chorionic gonadotropin / gap junction-mediated intercellular transport / gap junction channel activity involved in cell communication by electrical coupling / epididymis development / Oligomerization of connexins into connexons / Transport of connexins along the secretory pathway / gap junction assembly / connexin complex / gap junction / astrocyte projection / Gap junction assembly / gap junction channel activity / cellular response to glucagon stimulus / inner ear development / endoplasmic reticulum-Golgi intermediate compartment / decidualization / lateral plasma membrane / response to retinoic acid / cellular response to dexamethasone stimulus / response to ischemia / response to progesterone / sensory perception of sound / transmembrane transport / response to estradiol / cell-cell signaling / cellular response to oxidative stress / cell body / response to lipopolysaccharide / calcium ion binding / perinuclear region of cytoplasm / identical protein binding / plasma membrane 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.1 Å | |||||||||
データ登録者 | Brotherton, D.H. / Cameron, A.D. / Savva, C.G. / Ragan, T.J. | |||||||||
資金援助 | 英国, 2件
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引用 | ジャーナル: Structure / 年: 2022 タイトル: Conformational changes and CO-induced channel gating in connexin26. 著者: Deborah H Brotherton / Christos G Savva / Timothy J Ragan / Nicholas Dale / Alexander D Cameron / 要旨: Connexins form large-pore channels that function either as dodecameric gap junctions or hexameric hemichannels to allow the regulated movement of small molecules and ions across cell membranes. ...Connexins form large-pore channels that function either as dodecameric gap junctions or hexameric hemichannels to allow the regulated movement of small molecules and ions across cell membranes. Opening or closing of the channels is controlled by a variety of stimuli, and dysregulation leads to multiple diseases. An increase in the partial pressure of carbon dioxide (PCO) has been shown to cause connexin26 (Cx26) gap junctions to close. Here, we use cryoelectron microscopy (cryo-EM) to determine the structure of human Cx26 gap junctions under increasing levels of PCO. We show a correlation between the level of PCO and the size of the aperture of the pore, governed by the N-terminal helices that line the pore. This indicates that CO alone is sufficient to cause conformational changes in the protein. Analysis of the conformational states shows that movements at the N terminus are linked to both subunit rotation and flexing of the transmembrane helices. | |||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 7qet.cif.gz | 441.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb7qet.ent.gz | 356.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 7qet.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 7qet_validation.pdf.gz | 1.4 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 7qet_full_validation.pdf.gz | 1.4 MB | 表示 | |
XML形式データ | 7qet_validation.xml.gz | 77.2 KB | 表示 | |
CIF形式データ | 7qet_validation.cif.gz | 104.1 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/qe/7qet ftp://data.pdbj.org/pub/pdb/validation_reports/qe/7qet | HTTPS FTP |
-関連構造データ
関連構造データ | 13940MC 7qeoC 7qeqC 7qerC 7qesC 7qeuC 7qevC 7qewC 7qeyC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
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