EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational changes and CO-induced channel gating in connexin26.
ジャーナル・号・ページ	Structure, Vol. 30, Issue 5, Page 697-706.e4, Year 2022
掲載日	2022年5月5日
著者	Deborah H Brotherton / Christos G Savva / Timothy J Ragan / Nicholas Dale / Alexander D Cameron /
PubMed 要旨	Connexins form large-pore channels that function either as dodecameric gap junctions or hexameric hemichannels to allow the regulated movement of small molecules and ions across cell membranes. ...Connexins form large-pore channels that function either as dodecameric gap junctions or hexameric hemichannels to allow the regulated movement of small molecules and ions across cell membranes. Opening or closing of the channels is controlled by a variety of stimuli, and dysregulation leads to multiple diseases. An increase in the partial pressure of carbon dioxide (PCO) has been shown to cause connexin26 (Cx26) gap junctions to close. Here, we use cryoelectron microscopy (cryo-EM) to determine the structure of human Cx26 gap junctions under increasing levels of PCO. We show a correlation between the level of PCO and the size of the aperture of the pore, governed by the N-terminal helices that line the pore. This indicates that CO alone is sufficient to cause conformational changes in the protein. Analysis of the conformational states shows that movements at the N terminus are linked to both subunit rotation and flexing of the transmembrane helices.
リンク	Structure / PubMed:35276081 / PubMed Central
手法	EM (単粒子)
解像度	1.9 - 2.9 Å
構造データ	13935 7qeo EMDB-13935, PDB-7qeo: human Connexin 26 at 55mm Hg PCO2, pH7.4: two masked subunits, class C 手法: EM (単粒子) / 解像度: 2.9 Å 13937 7qeq EMDB-13937, PDB-7qeq: human Connexin 26 dodecamer at 90mmHg PCO2, pH7.4 手法: EM (単粒子) / 解像度: 1.9 Å 13938 7qer EMDB-13938, PDB-7qer: human Connexin 26 dodecamer at 55mm Hg PCO2, pH7.4 手法: EM (単粒子) / 解像度: 2.2 Å 13939 7qes EMDB-13939, PDB-7qes: human Connexin 26 at 55mm Hg PCO2, pH7.4: two masked subunits, class A 手法: EM (単粒子) / 解像度: 2.6 Å 13940 7qet EMDB-13940, PDB-7qet: human Connexin 26 dodecamer at 20mmHg PCO2, pH7.4 手法: EM (単粒子) / 解像度: 2.1 Å 13941 7qeu EMDB-13941, PDB-7qeu: human Connexin 26 at 55mmHg PCO2, pH7.4: two masked subunits, class B 手法: EM (単粒子) / 解像度: 2.7 Å 13942 7qev EMDB-13942, PDB-7qev: human Connexin 26 at 55mm Hg PCO2, pH7.4:two masked subunits, class D 手法: EM (単粒子) / 解像度: 2.9 Å 13943 7qew EMDB-13943, PDB-7qew: human Connexin 26 class 2 hexamer at 90mmHg PCO2, pH7.4 手法: EM (単粒子) / 解像度: 2.1 Å 13944 7qey EMDB-13944, PDB-7qey: human Connexin 26 class 1 hexamer at 90mmHg PCO2, pH7.4 手法: EM (単粒子) / 解像度: 2.0 Å
化合物	ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-HOH: WATER / 水
由来	homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Gap junction (ギャップ結合) / Ion Channel (イオンチャネル) / carbon dioxide sensitive