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基本情報
登録情報 | データベース: PDB / ID: 7ktr | |||||||||||||||||||||
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タイトル | Cryo-EM structure of the human SAGA coactivator complex (TRRAP, core) | |||||||||||||||||||||
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![]() | TRANSCRIPTION / coactivator / gene regulation / chromatin | |||||||||||||||||||||
機能・相同性 | ![]() SAGA-type complex / positive regulation of response to stimulus / regulation of somatic stem cell population maintenance / regulation of cellular response to stress / SAGA complex assembly / lateral mesodermal cell differentiation / allantois development / transcription factor TFTC complex / SLIK (SAGA-like) complex / negative regulation of microtubule depolymerization ...SAGA-type complex / positive regulation of response to stimulus / regulation of somatic stem cell population maintenance / regulation of cellular response to stress / SAGA complex assembly / lateral mesodermal cell differentiation / allantois development / transcription factor TFTC complex / SLIK (SAGA-like) complex / negative regulation of microtubule depolymerization / hepatocyte differentiation / maintenance of protein location in nucleus / RNA polymerase binding / SAGA complex / limb development / transcription preinitiation complex / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / NuA4 histone acetyltransferase complex / nucleus organization / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / regulation of RNA splicing / histone deacetylase complex / transcription factor TFIID complex / RNA polymerase II general transcription initiation factor activity / histone acetyltransferase complex / embryonic placenta development / positive regulation of transcription initiation by RNA polymerase II / regulation of DNA repair / somitogenesis / RNA polymerase II preinitiation complex assembly / gastrulation / RNA Polymerase II Pre-transcription Events / visual perception / TBP-class protein binding / male germ cell nucleus / DNA-templated transcription initiation / promoter-specific chromatin binding / nuclear estrogen receptor binding / transcription initiation at RNA polymerase II promoter / transcription coregulator activity / mRNA transcription by RNA polymerase II / multicellular organism growth / autophagy / cytoplasmic ribonucleoprotein granule / nuclear matrix / microtubule cytoskeleton organization / G1/S transition of mitotic cell cycle / transcription corepressor activity / microtubule cytoskeleton / HATs acetylate histones / positive regulation of cell growth / DNA-binding transcription factor binding / Regulation of TP53 Activity through Phosphorylation / transcription by RNA polymerase II / transcription coactivator activity / protein stabilization / Ub-specific processing proteases / nuclear speck / chromatin remodeling / protein heterodimerization activity / DNA repair / focal adhesion / apoptotic process / regulation of DNA-templated transcription / nucleolus / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / perinuclear region of cytoplasm / Golgi apparatus / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / extracellular exosome / nucleoplasm / identical protein binding / nucleus / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||||||||||||||
生物種 | ![]() ![]() | |||||||||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.93 Å | |||||||||||||||||||||
![]() | Herbst, D.A. / Esbin, M.N. / Nogales, E. | |||||||||||||||||||||
資金援助 | ![]() ![]()
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![]() | ![]() タイトル: Structure of the human SAGA coactivator complex. 著者: Dominik A Herbst / Meagan N Esbin / Robert K Louder / Claire Dugast-Darzacq / Gina M Dailey / Qianglin Fang / Xavier Darzacq / Robert Tjian / Eva Nogales / ![]() ![]() 要旨: The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification, DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been reported, there are ...The SAGA complex is a regulatory hub involved in gene regulation, chromatin modification, DNA damage repair and signaling. While structures of yeast SAGA (ySAGA) have been reported, there are noteworthy functional and compositional differences for this complex in metazoans. Here we present the cryogenic-electron microscopy (cryo-EM) structure of human SAGA (hSAGA) and show how the arrangement of distinct structural elements results in a globally divergent organization from that of yeast, with a different interface tethering the core module to the TRRAP subunit, resulting in a dramatically altered geometry of functional elements and with the integration of a metazoan-specific splicing module. Our hSAGA structure reveals the presence of an inositol hexakisphosphate (InsP) binding site in TRRAP and an unusual property of its pseudo-(Ψ)PIKK. Finally, we map human disease mutations, thus providing the needed framework for structure-guided drug design of this important therapeutic target for human developmental diseases and cancer. | |||||||||||||||||||||
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-検証レポート
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-関連構造データ
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集合体
登録構造単位 | ![]()
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要素
-タンパク質 , 8種, 8分子 ABCDFIJN
#1: タンパク質 | 分子量: 353170.281 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
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#2: タンパク質 | 分子量: 66223.047 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
#3: タンパク質 | 分子量: 88129.070 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
#4: タンパク質 | 分子量: 78079.922 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) ![]() ![]() 遺伝子: SUPT7L, KIAA0764 / 発現宿主: ![]() |
#6: タンパク質 | 分子量: 67903.289 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
#9: タンパク質 | 分子量: 33197.047 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
#10: タンパク質 | 分子量: 37432.531 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
#11: タンパク質 | 分子量: 95597.742 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
-Transcription initiation factor TFIID subunit ... , 3種, 3分子 EGH
#5: タンパク質 | 分子量: 27654.861 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
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#7: タンパク質 | 分子量: 17948.467 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
#8: タンパク質 | 分子量: 21731.248 Da / 分子数: 1 / 由来タイプ: 天然 / 由来: (天然) ![]() |
-非ポリマー , 1種, 1分子 ![](data/chem/img/IHP.gif)
#12: 化合物 | ChemComp-IHP / |
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-詳細
研究の焦点であるリガンドがあるか | Y |
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配列の詳細 | Portions of chains A, D and I were not fully discernible in the map, and so many of the residues ...Portions of chains A, D and I were not fully discernible in the map, and so many of the residues were modeled as unknown (UNK) due to not knowing the register in these regions. The full sequence of the chains are as follows. Chain A: MAFVATQGAT |