National Natural Science Foundation of China (NSFC)
No. 31941011, 12034006, 31672592, 31873023, 31811540395, 31772739, 315725153 and 1811540395
China
Citation
Journal: Protein Cell / Year: 2022 Title: Structural and molecular basis for foot-and-mouth disease virus neutralization by two potent protective antibodies. Authors: Hu Dong / Pan Liu / Manyuan Bai / Kang Wang / Rui Feng / Dandan Zhu / Yao Sun / Suyu Mu / Haozhou Li / Michiel Harmsen / Shiqi Sun / Xiangxi Wang / Huichen Guo /
A: M8 Nab 1: VP1 of O type FMDV capsid 2: VP2 of O-type FMDV capsid 3: VP3 of O-type FMDV capsid 4: VP4 of O-type FMDV capsid
x 5
icosahedral pentamer
458 kDa, 25 polymers
Theoretical mass
Number of molelcules
Total (without water)
457,704
25
Polymers
457,704
25
Non-polymers
0
0
Water
0
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: M8 Nab 1: VP1 of O type FMDV capsid 2: VP2 of O-type FMDV capsid 3: VP3 of O-type FMDV capsid 4: VP4 of O-type FMDV capsid
x 6
icosahedral 23 hexamer
549 kDa, 30 polymers
Theoretical mass
Number of molelcules
Total (without water)
549,244
30
Polymers
549,244
30
Non-polymers
0
0
Water
0
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
point symmetry operation
5
5
Idetical with deposited unit in distinct coordinate
icosahedral asymmetric unit, std point frame
Type
Name
Symmetry operation
Number
transform to point frame
1
Symmetry
Point symmetry: (Schoenflies symbol: I (icosahedral))
-
Components
#1: Protein
M8Nab
Mass: 13932.477 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Lama glama (llama)
#2: Protein
VP1ofOtypeFMDVcapsid
Mass: 22849.139 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus
#3: Protein
VP2ofO-typeFMDVcapsid
Mass: 23091.078 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus
#4: Protein
VP3ofO-typeFMDVcapsid
Mass: 23704.688 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus
#5: Protein
VP4ofO-typeFMDVcapsid
Mass: 7963.328 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Foot-and-mouth disease virus
-
Experimental details
-
Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction
-
Sample preparation
Component
ID
Name
Type
Entity ID
Parent-ID
Source
1
FMDVincomplexwithM8Nab
COMPLEX
all
0
MULTIPLESOURCES
2
M8Nab
COMPLEX
#1
1
RECOMBINANT
3
FMDV
COMPLEX
#2-#5
1
NATURAL
Source (natural)
ID
Entity assembly-ID
Organism
Ncbi tax-ID
2
1
Vicugna pacos (alpaca)
30538
3
2
Foot-and-mouth disease virus
12110
Source (recombinant)
Organism: Lama glama (llama)
Buffer solution
pH: 7.4
Specimen
Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Vitrification
Cryogen name: ETHANE
-
Electron microscopy imaging
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
Microscopy
Model: FEI TALOS ARCTICA
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lens
Mode: DARK FIELD
Image recording
Electron dose: 30 e/Å2 / Film or detector model: GATAN K2 QUANTUM (4k x 4k)
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
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