ジャーナル: Structure / 年: 2022 タイトル: Acid-stable capsid structure of Helicobacter pylori bacteriophage KHP30 by single-particle cryoelectron microscopy. 著者: Ryosuke Kamiya / Jumpei Uchiyama / Shigenobu Matsuzaki / Kazuyoshi Murata / Kenji Iwasaki / Naoyuki Miyazaki / 要旨: The acid-stable capsid structures of Helicobacter pylori phages KHP30 and KHP40 are solved at 2.7 and 3.0 Å resolutions by cryoelectron microscopy, respectively. The capsids have icosahedral T = 9 ...The acid-stable capsid structures of Helicobacter pylori phages KHP30 and KHP40 are solved at 2.7 and 3.0 Å resolutions by cryoelectron microscopy, respectively. The capsids have icosahedral T = 9 symmetry and consist of each 540 copies of 2 structural proteins, a major capsid protein, and a cement protein. The major capsid proteins form 12 pentagonal capsomeres occupying icosahedral vertexes and 80 hexagonal capsomeres located at icosahedral faces and edges. The major capsid protein has a unique protruding loop extending to the neighboring subunit that stabilizes hexagonal capsomeres. Furthermore, the capsid is decorated with trimeric cement proteins with a jelly roll motif. The cement protein trimer sits on the quasi-three-fold axis formed by three major capsid protein capsomeres, thereby enhancing the particle stability by connecting these capsomeres. Sequence and structure comparisons between the related Helicobacter pylori phages suggest a possible mechanism of phage adaptation to the human gastric environment.
a: Major structural protein ORF14 b: Major structural protein ORF14 c: Major structural protein ORF14 d: Major structural protein ORF14 e: Major structural protein ORF14 f: Major structural protein ORF14 g: Major structural protein ORF14 h: Major structural protein ORF14 i: Major structural protein ORF14 1: Cement protein gp15 2: Cement protein gp15 3: Cement protein gp15 4: Cement protein gp15 5: Cement protein gp15 6: Cement protein gp15 7: Cement protein gp15 8: Cement protein gp15 9: Cement protein gp15
a: Major structural protein ORF14 b: Major structural protein ORF14 c: Major structural protein ORF14 d: Major structural protein ORF14 e: Major structural protein ORF14 f: Major structural protein ORF14 g: Major structural protein ORF14 h: Major structural protein ORF14 i: Major structural protein ORF14 1: Cement protein gp15 2: Cement protein gp15 3: Cement protein gp15 4: Cement protein gp15 5: Cement protein gp15 6: Cement protein gp15 7: Cement protein gp15 8: Cement protein gp15 9: Cement protein gp15
a: Major structural protein ORF14 b: Major structural protein ORF14 c: Major structural protein ORF14 d: Major structural protein ORF14 e: Major structural protein ORF14 f: Major structural protein ORF14 g: Major structural protein ORF14 h: Major structural protein ORF14 i: Major structural protein ORF14 1: Cement protein gp15 2: Cement protein gp15 3: Cement protein gp15 4: Cement protein gp15 5: Cement protein gp15 6: Cement protein gp15 7: Cement protein gp15 8: Cement protein gp15 9: Cement protein gp15
x 5
icosahedral pentamer
2.53 MDa, 90 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
2,528,432
90
ポリマ-
2,528,432
90
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
a: Major structural protein ORF14 b: Major structural protein ORF14 c: Major structural protein ORF14 d: Major structural protein ORF14 e: Major structural protein ORF14 f: Major structural protein ORF14 g: Major structural protein ORF14 h: Major structural protein ORF14 i: Major structural protein ORF14 1: Cement protein gp15 2: Cement protein gp15 3: Cement protein gp15 4: Cement protein gp15 5: Cement protein gp15 6: Cement protein gp15 7: Cement protein gp15 8: Cement protein gp15 9: Cement protein gp15