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-Basic information
Entry | Database: PDB / ID: 7art | |||||||||||||||
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Title | 48 helix bundle DNA origami brick | |||||||||||||||
Components |
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Keywords | DNA / DNA Origami | |||||||||||||||
Function / homology | DNA / DNA (> 10) / DNA (> 100) / DNA (> 1000) Function and homology information | |||||||||||||||
Biological species | synthetic construct (others) | |||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 10 Å | |||||||||||||||
Authors | Feigl, E. / Kube, M. / Kohler, F. / Nagel-Yuksel, B. / Willner, E.M. / Funke, J.J. / Gerling, T. / Stommer, P. / Honemann, M.N. / Martin, T.G. ...Feigl, E. / Kube, M. / Kohler, F. / Nagel-Yuksel, B. / Willner, E.M. / Funke, J.J. / Gerling, T. / Stommer, P. / Honemann, M.N. / Martin, T.G. / Scheres, S.H.W. / Dietz, H. | |||||||||||||||
Funding support | Germany, 2items
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Citation | Journal: Nat Commun / Year: 2020 Title: Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution. Authors: Massimo Kube / Fabian Kohler / Elija Feigl / Baki Nagel-Yüksel / Elena M Willner / Jonas J Funke / Thomas Gerling / Pierre Stömmer / Maximilian N Honemann / Thomas G Martin / Sjors H W ...Authors: Massimo Kube / Fabian Kohler / Elija Feigl / Baki Nagel-Yüksel / Elena M Willner / Jonas J Funke / Thomas Gerling / Pierre Stömmer / Maximilian N Honemann / Thomas G Martin / Sjors H W Scheres / Hendrik Dietz / Abstract: The methods of DNA nanotechnology enable the rational design of custom shapes that self-assemble in solution from sets of DNA molecules. DNA origami, in which a long template DNA single strand is ...The methods of DNA nanotechnology enable the rational design of custom shapes that self-assemble in solution from sets of DNA molecules. DNA origami, in which a long template DNA single strand is folded by many short DNA oligonucleotides, can be employed to make objects comprising hundreds of unique DNA strands and thousands of base pairs, thus in principle providing many degrees of freedom for modelling complex objects of defined 3D shapes and sizes. Here, we address the problem of accurate structural validation of DNA objects in solution with cryo-EM based methodologies. By taking into account structural fluctuations, we can determine structures with improved detail compared to previous work. To interpret the experimental cryo-EM maps, we present molecular-dynamics-based methods for building pseudo-atomic models in a semi-automated fashion. Among other features, our data allows discerning details such as helical grooves, single-strand versus double-strand crossovers, backbone phosphate positions, and single-strand breaks. Obtaining this higher level of detail is a step forward that now allows designers to inspect and refine their designs with base-pair level interventions. | |||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 7art.cif.gz | 6.7 MB | Display | PDBx/mmCIF format |
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PDB format | pdb7art.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 7art.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7art_validation.pdf.gz | 2.7 MB | Display | wwPDB validaton report |
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Full document | 7art_full_validation.pdf.gz | 3.4 MB | Display | |
Data in XML | 7art_validation.xml.gz | 219.4 KB | Display | |
Data in CIF | 7art_validation.cif.gz | 516 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ar/7art ftp://data.pdbj.org/pub/pdb/validation_reports/ar/7art | HTTPS FTP |
-Related structure data
Related structure data | 11387MC 7areC 7arqC 7arvC 7aryC 7as5C M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit | AA: SCAFFOLD STRAND AB: STAPLE STRAND AC: STAPLE STRAND AD: STAPLE STRAND AE: STAPLE STRAND AF: STAPLE STRAND AG: STAPLE STRAND AH: STAPLE STRAND AI: STAPLE STRAND AJ: STAPLE STRAND AK: STAPLE STRAND AL: STAPLE STRAND AM: STAPLE STRAND AN: STAPLE STRAND AO: STAPLE STRAND AP: STAPLE STRAND AQ: STAPLE STRAND AR: STAPLE STRAND AS: STAPLE STRAND AT: STAPLE STRAND AU: STAPLE STRAND AV: STAPLE STRAND AW: STAPLE STRAND AX: STAPLE STRAND AY: STAPLE STRAND AZ: STAPLE STRAND Aa: STAPLE STRAND Ab: STAPLE STRAND Ac: STAPLE STRAND Ad: STAPLE STRAND Ae: STAPLE STRAND Af: STAPLE STRAND Ag: STAPLE STRAND Ah: STAPLE STRAND Ai: STAPLE STRAND Aj: STAPLE STRAND Ak: STAPLE STRAND Al: STAPLE STRAND Am: STAPLE STRAND An: STAPLE STRAND Ao: STAPLE STRAND Ap: STAPLE STRAND Aq: STAPLE STRAND Ar: STAPLE STRAND As: STAPLE STRAND At: STAPLE STRAND Au: STAPLE STRAND Av: STAPLE STRAND Aw: STAPLE STRAND Ax: STAPLE STRAND Ay: STAPLE STRAND Az: STAPLE STRAND A0: STAPLE STRAND A1: STAPLE STRAND A2: STAPLE STRAND A3: STAPLE STRAND A4: STAPLE STRAND A5: STAPLE STRAND A6: STAPLE STRAND A7: STAPLE STRAND A8: STAPLE STRAND A9: STAPLE STRAND BA: STAPLE STRAND BB: STAPLE STRAND BC: STAPLE STRAND BD: STAPLE STRAND BE: STAPLE STRAND BF: STAPLE STRAND BG: STAPLE STRAND BH: STAPLE STRAND BI: STAPLE STRAND BJ: STAPLE STRAND BK: STAPLE STRAND BL: STAPLE STRAND BM: STAPLE STRAND BN: STAPLE STRAND BO: STAPLE STRAND BP: STAPLE STRAND BQ: STAPLE STRAND BR: STAPLE STRAND BS: STAPLE STRAND BT: STAPLE STRAND BU: STAPLE STRAND BV: STAPLE STRAND BW: STAPLE STRAND BX: STAPLE STRAND BY: STAPLE STRAND BZ: STAPLE STRAND Ba: STAPLE STRAND Bb: STAPLE STRAND Bc: STAPLE STRAND Bd: STAPLE STRAND Be: STAPLE STRAND Bf: STAPLE STRAND Bg: STAPLE STRAND Bh: STAPLE STRAND Bi: STAPLE STRAND Bj: STAPLE STRAND Bk: STAPLE STRAND Bl: STAPLE STRAND Bm: STAPLE STRAND Bn: STAPLE STRAND Bo: STAPLE STRAND Bp: STAPLE STRAND Bq: STAPLE STRAND Br: STAPLE STRAND Bs: STAPLE STRAND Bt: STAPLE STRAND Bu: STAPLE STRAND Bv: STAPLE STRAND Bw: STAPLE STRAND Bx: STAPLE STRAND By: STAPLE STRAND Bz: STAPLE STRAND B0: STAPLE STRAND B1: STAPLE STRAND B2: STAPLE STRAND B3: STAPLE STRAND B4: STAPLE STRAND B5: STAPLE STRAND B6: STAPLE STRAND B7: STAPLE STRAND B8: STAPLE STRAND B9: STAPLE STRAND CA: STAPLE STRAND CB: STAPLE STRAND CC: STAPLE STRAND CD: STAPLE STRAND CE: STAPLE STRAND CF: STAPLE STRAND CG: STAPLE STRAND CH: STAPLE STRAND CI: STAPLE STRAND CJ: STAPLE STRAND CK: STAPLE STRAND CL: STAPLE STRAND CM: STAPLE STRAND CN: STAPLE STRAND CO: STAPLE STRAND CP: STAPLE STRAND CQ: STAPLE STRAND CR: STAPLE STRAND CS: STAPLE STRAND CT: STAPLE STRAND CU: STAPLE STRAND CV: STAPLE STRAND CW: STAPLE STRAND CX: STAPLE STRAND CY: STAPLE STRAND CZ: STAPLE STRAND Ca: STAPLE STRAND Cb: STAPLE STRAND Cc: STAPLE STRAND Cd: STAPLE STRAND Ce: STAPLE STRAND Cf: STAPLE STRAND Cg: STAPLE STRAND Ch: STAPLE STRAND Ci: STAPLE STRAND Cj: STAPLE STRAND Ck: STAPLE STRAND Cl: STAPLE STRAND Cm: STAPLE STRAND Cn: STAPLE STRAND Co: STAPLE STRAND Cp: STAPLE STRAND Cq: STAPLE STRAND Cr: STAPLE STRAND Cs: STAPLE STRAND Ct: STAPLE STRAND Cu: STAPLE STRAND Cv: STAPLE STRAND Cw: STAPLE STRAND Cx: STAPLE STRAND Cy: STAPLE STRAND Cz: STAPLE STRAND C0: STAPLE STRAND C1: STAPLE STRAND C2: STAPLE STRAND C3: STAPLE STRAND C4: STAPLE STRAND C5: STAPLE STRAND C6: STAPLE STRAND C7: STAPLE STRAND C8: STAPLE STRAND C9: STAPLE STRAND DA: STAPLE STRAND DB: STAPLE STRAND DC: STAPLE STRAND DD: STAPLE STRAND DE: STAPLE STRAND DF: STAPLE STRAND DG: STAPLE STRAND DH: STAPLE STRAND DI: STAPLE STRAND DJ: STAPLE STRAND DK: STAPLE STRAND DL: STAPLE STRAND DM: STAPLE STRAND DN: STAPLE STRAND DO: STAPLE STRAND DP: STAPLE STRAND DQ: STAPLE STRAND DR: STAPLE STRAND DS: STAPLE STRAND DT: STAPLE STRAND DU: STAPLE STRAND DV: STAPLE STRAND DW: STAPLE STRAND DX: STAPLE STRAND DY: STAPLE STRAND DZ: STAPLE STRAND
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1 |
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-Components
+DNA chain , 212 types, 212 molecules AAABACADAEAFAGAHAIAJAKALAMANAOAPAQARASATAUAVAWAXAYAZAaAbAcAd...
#1: DNA chain | Mass: 2488158.250 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
---|---|
#2: DNA chain | Mass: 12272.905 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#3: DNA chain | Mass: 13423.601 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#4: DNA chain | Mass: 15547.976 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#5: DNA chain | Mass: 14352.249 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#6: DNA chain | Mass: 15169.768 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#7: DNA chain | Mass: 15018.687 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#8: DNA chain | Mass: 15235.794 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#9: DNA chain | Mass: 15137.720 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#10: DNA chain | Mass: 12953.351 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#11: DNA chain | Mass: 16705.730 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#12: DNA chain | Mass: 11624.460 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#13: DNA chain | Mass: 13500.686 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#14: DNA chain | Mass: 10739.949 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#15: DNA chain | Mass: 12184.833 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#16: DNA chain | Mass: 12854.285 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#17: DNA chain | Mass: 10932.042 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#18: DNA chain | Mass: 15240.839 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#19: DNA chain | Mass: 12784.240 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#20: DNA chain | Mass: 11658.540 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#21: DNA chain | Mass: 14523.438 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#22: DNA chain | Mass: 11335.298 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#23: DNA chain | Mass: 16741.850 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#24: DNA chain | Mass: 11039.095 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#25: DNA chain | Mass: 11093.142 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#26: DNA chain | Mass: 13354.612 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#27: DNA chain | Mass: 11705.550 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#28: DNA chain | Mass: 13086.493 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#29: DNA chain | Mass: 15161.758 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#30: DNA chain | Mass: 13106.407 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#31: DNA chain | Mass: 12918.424 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#32: DNA chain | Mass: 10899.003 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#33: DNA chain | Mass: 12265.922 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#34: DNA chain | Mass: 13019.429 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#35: DNA chain | Mass: 12989.315 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#36: DNA chain | Mass: 12760.215 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#37: DNA chain | Mass: 12930.366 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#38: DNA chain | Mass: 12883.304 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#39: DNA chain | Mass: 13497.667 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#40: DNA chain | Mass: 11592.462 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#41: DNA chain | Mass: 15036.767 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#42: DNA chain | Mass: 15136.819 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#43: DNA chain | Mass: 14317.219 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#44: DNA chain | Mass: 12817.280 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#45: DNA chain | Mass: 12923.378 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#46: DNA chain | Mass: 15828.127 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#47: DNA chain | Mass: 15116.709 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#48: DNA chain | Mass: 11434.362 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#49: DNA chain | Mass: 13063.404 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#50: DNA chain | Mass: 13359.549 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#51: DNA chain | Mass: 12215.842 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#52: DNA chain | Mass: 10930.029 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#53: DNA chain | Mass: 19365.443 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#54: DNA chain | Mass: 15000.661 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#55: DNA chain | Mass: 12994.414 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#56: DNA chain | Mass: 12291.919 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#57: DNA chain | Mass: 13455.650 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#58: DNA chain | Mass: 15422.948 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#59: DNA chain | Mass: 15200.829 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#60: DNA chain | Mass: 12812.271 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#61: DNA chain | Mass: 15309.863 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#62: DNA chain | Mass: 14996.654 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#63: DNA chain | Mass: 15082.770 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#64: DNA chain | Mass: 15186.811 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#65: DNA chain | Mass: 15028.688 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#66: DNA chain | Mass: 11648.539 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#67: DNA chain | Mass: 13518.713 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#68: DNA chain | Mass: 12971.431 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#69: DNA chain | Mass: 13066.472 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#70: DNA chain | Mass: 12272.961 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#71: DNA chain | Mass: 13130.484 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#72: DNA chain | Mass: 11547.392 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#73: DNA chain | Mass: 10140.524 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#74: DNA chain | Mass: 13121.486 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#75: DNA chain | Mass: 13257.506 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#76: DNA chain | Mass: 15768.205 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#77: DNA chain | Mass: 10759.967 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#78: DNA chain | Mass: 12258.971 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#79: DNA chain | Mass: 11416.389 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#80: DNA chain | Mass: 11673.498 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#81: DNA chain | Mass: 14480.311 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#82: DNA chain | Mass: 11514.465 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#83: DNA chain | Mass: 12191.870 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#84: DNA chain | Mass: 12936.399 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#85: DNA chain | Mass: 10700.965 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#86: DNA chain | Mass: 12914.366 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#87: DNA chain | Mass: 10737.973 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#88: DNA chain | Mass: 12919.359 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#89: DNA chain | Mass: 15086.689 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#90: DNA chain | Mass: 13323.549 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#91: DNA chain | Mass: 11616.487 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#92: DNA chain | Mass: 12848.345 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#93: DNA chain | Mass: 14962.665 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#94: DNA chain | Mass: 13046.363 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#95: DNA chain | Mass: 15152.781 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#96: DNA chain | Mass: 15714.158 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#97: DNA chain | Mass: 10746.933 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#98: DNA chain | Mass: 11358.335 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#99: DNA chain | Mass: 12818.268 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#100: DNA chain | Mass: 13394.583 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#101: DNA chain | Mass: 12244.930 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#102: DNA chain | Mass: 19435.473 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#103: DNA chain | Mass: 10894.048 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#104: DNA chain | Mass: 12945.377 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#105: DNA chain | Mass: 12977.375 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#106: DNA chain | Mass: 15156.748 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#107: DNA chain | Mass: 10771.999 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#108: DNA chain | Mass: 10792.953 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#109: DNA chain | Mass: 12337.994 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#110: DNA chain | Mass: 13437.621 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#111: DNA chain | Mass: 15216.830 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#112: DNA chain | Mass: 14486.345 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#113: DNA chain | Mass: 12222.863 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#114: DNA chain | Mass: 15286.876 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#115: DNA chain | Mass: 13147.489 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#116: DNA chain | Mass: 15293.793 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#117: DNA chain | Mass: 11633.475 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#118: DNA chain | Mass: 13458.683 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#119: DNA chain | Mass: 10637.868 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#120: DNA chain | Mass: 10735.944 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#121: DNA chain | Mass: 15025.671 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#122: DNA chain | Mass: 15200.831 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#123: DNA chain | Mass: 12999.354 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#124: DNA chain | Mass: 11017.115 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#125: DNA chain | Mass: 10787.944 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#126: DNA chain | Mass: 10704.934 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#127: DNA chain | Mass: 15168.779 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#128: DNA chain | Mass: 12727.230 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#129: DNA chain | Mass: 10943.085 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#130: DNA chain | Mass: 11573.445 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#131: DNA chain | Mass: 15766.174 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#132: DNA chain | Mass: 15378.920 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#133: DNA chain | Mass: 13053.382 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#134: DNA chain | Mass: 12160.843 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#135: DNA chain | Mass: 11498.463 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#136: DNA chain | Mass: 14477.383 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#137: DNA chain | Mass: 11633.474 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#138: DNA chain | Mass: 12412.030 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#139: DNA chain | Mass: 12901.333 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#140: DNA chain | Mass: 10755.946 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#141: DNA chain | Mass: 12384.952 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#142: DNA chain | Mass: 10775.897 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#143: DNA chain | Mass: 15249.852 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#144: DNA chain | Mass: 13123.448 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#145: DNA chain | Mass: 13315.575 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#146: DNA chain | Mass: 11609.449 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#147: DNA chain | Mass: 12925.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#148: DNA chain | Mass: 13036.414 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#149: DNA chain | Mass: 15141.792 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#150: DNA chain | Mass: 15074.656 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#151: DNA chain | Mass: 13033.397 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#152: DNA chain | Mass: 15172.733 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#153: DNA chain | Mass: 12867.358 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#154: DNA chain | Mass: 14492.394 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#155: DNA chain | Mass: 13477.699 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#156: DNA chain | Mass: 12443.939 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#157: DNA chain | Mass: 11039.096 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#158: DNA chain | Mass: 12867.255 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#159: DNA chain | Mass: 17249.145 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#160: DNA chain | Mass: 10742.911 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#161: DNA chain | Mass: 10950.034 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#162: DNA chain | Mass: 12471.031 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#163: DNA chain | Mass: 13558.682 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#164: DNA chain | Mass: 12835.200 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#165: DNA chain | Mass: 10689.868 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#166: DNA chain | Mass: 15145.691 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#167: DNA chain | Mass: 13420.604 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#168: DNA chain | Mass: 15189.721 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#169: DNA chain | Mass: 13569.745 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#170: DNA chain | Mass: 12971.325 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#171: DNA chain | Mass: 11639.472 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#172: DNA chain | Mass: 13360.555 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#173: DNA chain | Mass: 10648.855 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#174: DNA chain | Mass: 10935.005 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#175: DNA chain | Mass: 13019.374 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#176: DNA chain | Mass: 15125.671 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#177: DNA chain | Mass: 12376.926 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#178: DNA chain | Mass: 12982.369 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#179: DNA chain | Mass: 15133.749 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#180: DNA chain | Mass: 15163.772 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#181: DNA chain | Mass: 13037.366 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#182: DNA chain | Mass: 12839.275 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#183: DNA chain | Mass: 11634.462 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#184: DNA chain | Mass: 12196.861 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#185: DNA chain | Mass: 11079.117 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#186: DNA chain | Mass: 15240.714 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#187: DNA chain | Mass: 13723.789 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#188: DNA chain | Mass: 12894.346 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#189: DNA chain | Mass: 10677.875 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#190: DNA chain | Mass: 14474.350 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#191: DNA chain | Mass: 11412.367 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#192: DNA chain | Mass: 11655.471 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#193: DNA chain | Mass: 14953.543 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#194: DNA chain | Mass: 13035.374 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#195: DNA chain | Mass: 12465.001 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#196: DNA chain | Mass: 12851.255 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#197: DNA chain | Mass: 10793.979 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#198: DNA chain | Mass: 13450.605 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#199: DNA chain | Mass: 11667.448 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#200: DNA chain | Mass: 13075.383 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#201: DNA chain | Mass: 14957.566 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#202: DNA chain | Mass: 10721.867 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#203: DNA chain | Mass: 15106.708 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#204: DNA chain | Mass: 15372.852 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#205: DNA chain | Mass: 14974.605 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#206: DNA chain | Mass: 11394.286 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#207: DNA chain | Mass: 12911.246 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#208: DNA chain | Mass: 15253.731 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#209: DNA chain | Mass: 13483.644 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#210: DNA chain | Mass: 12275.871 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#211: DNA chain | Mass: 12921.301 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
#212: DNA chain | Mass: 12871.328 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: 48 helix bundle DNA origami brick , multilayer DNA origami object Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: synthetic construct (others) |
Source (recombinant) | Organism: synthetic construct (others) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 50 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON III (4k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
---|---|
3D reconstruction | Resolution: 10 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 31931 / Symmetry type: POINT |
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