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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7abg | ||||||
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タイトル | Human pre-Bact-1 spliceosome | ||||||
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![]() | SPLICING / Complex / spliceosome / catalytic activation | ||||||
機能・相同性 | ![]() positive regulation of RNA binding / snRNA export from nucleus / nuclear cap binding complex / DNA topoisomerase binding / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / microfibril / Lsm2-8 complex / U6 snRNA 3'-end binding ...positive regulation of RNA binding / snRNA export from nucleus / nuclear cap binding complex / DNA topoisomerase binding / mRNA metabolic process / RNA cap binding complex / histone mRNA metabolic process / microfibril / Lsm2-8 complex / U6 snRNA 3'-end binding / positive regulation of mRNA 3'-end processing / positive regulation of RNA export from nucleus / protein kinase B binding / cap-dependent translational initiation / mRNA decay by 5' to 3' exoribonuclease / Lsm1-7-Pat1 complex / U6 snRNP / Processing of Intronless Pre-mRNAs / U11/U12 snRNP / snRNA binding / PH domain binding / regulation of retinoic acid receptor signaling pathway / RNA cap binding / WW domain binding / U2 snRNP binding / alternative mRNA splicing, via spliceosome / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / B-WICH complex / regulation of vitamin D receptor signaling pathway / histone pre-mRNA 3'end processing complex / miRNA-mediated post-transcriptional gene silencing / primary miRNA processing / cis assembly of pre-catalytic spliceosome / regulation of mRNA processing / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / mRNA splice site recognition / regulatory ncRNA-mediated post-transcriptional gene silencing / miRNA processing / Transport of the SLBP independent Mature mRNA / splicing factor binding / Transport of the SLBP Dependant Mature mRNA / RNA 7-methylguanosine cap binding / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / protein methylation / U12-type spliceosomal complex / methylosome / nuclear retinoic acid receptor binding / transcription elongation factor activity / 7-methylguanosine cap hypermethylation / Prp19 complex / positive regulation of androgen receptor activity / Transport of Mature mRNA Derived from an Intronless Transcript / snRNP binding / U1 snRNP binding / mRNA 3'-end processing / P-body assembly / pICln-Sm protein complex / blastocyst formation / mRNA 3'-end processing / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / pre-mRNA binding / U2-type catalytic step 1 spliceosome / sno(s)RNA-containing ribonucleoprotein complex / regulation of mRNA splicing, via spliceosome / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / telomerase holoenzyme complex / RNA catabolic process / mRNA cis splicing, via spliceosome / positive regulation by host of viral transcription / telomerase RNA binding / U2-type precatalytic spliceosome / U2-type spliceosomal complex / transcription regulator inhibitor activity / Transport of Mature mRNA derived from an Intron-Containing Transcript / Abortive elongation of HIV-1 transcript in the absence of Tat / commitment complex / positive regulation of vitamin D receptor signaling pathway / nuclear vitamin D receptor binding / U2-type prespliceosome assembly / RNA polymerase binding / U2-type catalytic step 2 spliceosome / ubiquitin-like protein conjugating enzyme binding / Notch binding / positive regulation of mRNA splicing, via spliceosome / regulation of translational initiation / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / U4 snRNP / RUNX3 regulates NOTCH signaling / RHOBTB1 GTPase cycle / U2 snRNP / FGFR2 alternative splicing / RNA Polymerase II Transcription Termination / SAGA complex / NOTCH4 Intracellular Domain Regulates Transcription 類似検索 - 分子機能 | ||||||
生物種 | ![]() synthetic construct (人工物) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.8 Å | ||||||
![]() | Townsend, C. / Kastner, B. / Leelaram, M.N. / Bertram, K. / Stark, H. / Luehrmann, R. | ||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation. 著者: Cole Townsend / Majety N Leelaram / Dmitry E Agafonov / Olexandr Dybkov / Cindy L Will / Karl Bertram / Henning Urlaub / Berthold Kastner / Holger Stark / Reinhard Lührmann / ![]() 要旨: Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway ...Spliceosome activation involves extensive protein and RNA rearrangements that lead to formation of a catalytically active U2/U6 RNA structure. At present, little is known about the assembly pathway of the latter and the mechanism whereby proteins aid its proper folding. Here, we report the cryo-electron microscopy structures of two human, activated spliceosome precursors (that is, pre-B complexes) at core resolutions of 3.9 and 4.2 angstroms. These structures elucidate the order of the numerous protein exchanges that occur during activation, the mutually exclusive interactions that ensure the correct order of ribonucleoprotein rearrangements needed to form the U2/U6 catalytic RNA, and the stepwise folding pathway of the latter. Structural comparisons with mature B complexes reveal the molecular mechanism whereby a conformational change in the scaffold protein PRP8 facilitates final three-dimensional folding of the U2/U6 catalytic RNA. | ||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 2.3 MB | 表示 | ![]() |
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PDB形式 | ![]() | 表示 | ![]() | |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
関連構造データ | ![]() 11695MC ![]() 7aavC ![]() 7abfC ![]() 7abhC ![]() 7abiC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data #1: Motion-corrected micrographs (without dose-weighting) of human pre-Bact spliceosome [micrographs - single frame] Data #2: Motion-corrected micrographs (with dose-weighting) of human pre-Bact spliceosome [micrographs - single frame]) |
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リンク
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集合体
登録構造単位 | ![]()
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要素
+Nuclear cap-binding protein subunit ... , 2種, 2分子 A5A1
+U6 snRNA-associated Sm-like protein ... , 7種, 7分子 A2V9CHJA3
+Splicing factor 3B subunit ... , 6種, 6分子 zuTEwx
+Splicing factor 3A subunit ... , 3種, 3分子 Fp4
+U5 small nuclear ribonucleoprotein ... , 2種, 2分子 Ds
+U2 small nuclear ribonucleoprotein ... , 2種, 2分子 WB
+タンパク質 , 17種, 18分子 QLRKyGAA4v0NrYA6mfqX
+RNA鎖 , 4種, 4分子 52Z6
+Pre-mRNA-splicing factor ... , 2種, 2分子 IP
+Small nuclear ribonucleoprotein ... , 6種, 12分子 haiblekdjcng
+非ポリマー , 4種, 4分子 ![](data/chem/img/IHP.gif)
![](data/chem/img/GTP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/GTG.gif)
![](data/chem/img/GTP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/GTG.gif)
+詳細
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 |
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分子量 | 単位: MEGADALTONS / 実験値: NO | ||||||||||||||||||
由来(天然) | 生物種: ![]() | ||||||||||||||||||
由来(組換発現) | 生物種: synthetic construct (人工物) | ||||||||||||||||||
緩衝液 | pH: 7.9 | ||||||||||||||||||
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||
試料支持 | グリッドの材料: COPPER / グリッドのタイプ: Quantifoil R3.5/1 | ||||||||||||||||||
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 平均露光時間: 1 sec. / 電子線照射量: 2.25 e/Å2 / 検出モード: INTEGRATING フィルム・検出器のモデル: FEI FALCON III (4k x 4k) |
画像スキャン | 横: 4096 / 縦: 4096 |
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解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||
3次元再構成 | 解像度: 7.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 84539 / 対称性のタイプ: POINT | ||||||||||||||||||||
原子モデル構築 | プロトコル: RIGID BODY FIT / 空間: REAL |