登録情報 データベース : PDB / ID : 6uro 構造の表示 ダウンロードとリンクタイトル Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex 要素(Cleavage and polyadenylation specificity factor subunit ...) x 2 Cleavage stimulation factor subunit 3 PAS RNA pre-mRNA 3' end processing protein WDR33 詳細キーワード RNA BINDING PROTEIN/RNA / pre-mRNA 3'-end processing / RNA binding / AAUAAA polyadenylation signal / RNA BINDING PROTEIN-RNA complex / mPSF機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
mRNA cleavage stimulating factor complex / co-transcriptional RNA 3'-end processing, cleavage and polyadenylation pathway / Inhibition of Host mRNA Processing and RNA Silencing / co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / RNA 3'-end processing / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / mRNA 3'-UTR AU-rich region binding / collagen trimer / mRNA 3'-end processing ... mRNA cleavage stimulating factor complex / co-transcriptional RNA 3'-end processing, cleavage and polyadenylation pathway / Inhibition of Host mRNA Processing and RNA Silencing / co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / RNA 3'-end processing / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / mRNA 3'-UTR AU-rich region binding / collagen trimer / mRNA 3'-end processing / Transport of Mature mRNA Derived from an Intronless Transcript / postreplication repair / tRNA processing in the nucleus / RNA Polymerase II Transcription Termination / : / Processing of Capped Intron-Containing Pre-mRNA / mRNA processing / fibrillar center / sequence-specific double-stranded DNA binding / spermatogenesis / intracellular membrane-bounded organelle / mRNA binding / enzyme binding / RNA binding / zinc ion binding / nucleoplasm / nucleus 類似検索 - 分子機能 mRNA 3'-end-processing protein Rna14-like / Zinc-finger CCCH domain / Zinc-finger containing family / Suppressor of forked / Suppressor of forked protein (Suf) / CPSF complex subunit CPSF4-like / Pre-mRNA 3' end processing protein Pfs2-like / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Zinc finger, CCCH-type superfamily / zinc finger ... mRNA 3'-end-processing protein Rna14-like / Zinc-finger CCCH domain / Zinc-finger containing family / Suppressor of forked / Suppressor of forked protein (Suf) / CPSF complex subunit CPSF4-like / Pre-mRNA 3' end processing protein Pfs2-like / Zinc finger C-x8-C-x5-C-x3-H type (and similar) / Zinc finger, CCCH-type superfamily / zinc finger / Zinc finger, CCCH-type / Zinc finger C3H1-type profile. / Collagen triple helix repeat / Collagen triple helix repeat (20 copies) / HAT (Half-A-TPR) repeat / HAT (Half-A-TPR) repeats / Cleavage/polyadenylation specificity factor, A subunit, N-terminal / Mono-functional DNA-alkylating methyl methanesulfonate N-term / Cleavage/polyadenylation specificity factor, A subunit, C-terminal / CPSF A subunit region / Tetratricopeptide repeat domain / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Alpha Horseshoe / Tetratricopeptide-like helical domain superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Mainly Alpha 類似検索 - ドメイン・相同性 RNA / RNA (> 10) / Cleavage and polyadenylation specificity factor subunit 4 / Cleavage and polyadenylation specificity factor subunit 1 / Cleavage stimulation factor subunit 3 / pre-mRNA 3' end processing protein WDR33 類似検索 - 構成要素生物種 Homo sapiens (ヒト) Macaca mulatta polyomavirus 1 (ウイルス)手法 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.6 Å 詳細データ登録者 Sun, Y. / Zhang, Y. / Walz, T. / Tong, L. 資金援助 米国, 1件 詳細 詳細を隠す組織 認可番号 国 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM118093 米国
引用ジャーナル : Mol Cell / 年 : 2020タイトル : Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.著者 : Yixiao Zhang / Yadong Sun / Yongsheng Shi / Thomas Walz / Liang Tong / 要旨 : The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall ... The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core. 履歴 登録 2019年10月23日 登録サイト : RCSB / 処理サイト : RCSB改定 1.0 2019年11月27日 Provider : repository / タイプ : Initial release改定 1.1 2019年12月18日 Group : Database references / カテゴリ : citationItem : _citation.country / _citation.journal_abbrev ... _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year 改定 1.2 2020年3月4日 Group : Database references / カテゴリ : citationItem : _citation.journal_volume / _citation.page_first / _citation.year改定 1.3 2024年3月20日 Group : Data collection / Database references / Refinement descriptionカテゴリ : chem_comp_atom / chem_comp_bond ... chem_comp_atom / chem_comp_bond / database_2 / em_3d_fitting_list / pdbx_initial_refinement_model Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession ... _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _em_3d_fitting_list.accession_code / _em_3d_fitting_list.initial_refinement_model_id / _em_3d_fitting_list.source_name / _em_3d_fitting_list.type
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