+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6s8h | ||||||
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タイトル | Cryo-EM structure of LptB2FG in complex with LPS | ||||||
要素 |
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キーワード | TRANSPORT PROTEIN / lipopolysaccharide transporter / LPS / LptB2FGC / LptB / LptBFG / outer membrane / Gram-negative bacteria / ABC transporter / Inner membrane protein complex | ||||||
機能・相同性 | 機能・相同性情報 トランスロカーゼ; 炭水化物とその誘導体の輸送を触媒; ヌクレオシド三リン酸の加水分解を伴う / lipopolysaccharide transport / ATP-binding cassette (ABC) transporter complex / transmembrane transport / ATP hydrolysis activity / ATP binding / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | Shigella flexneri (フレクスナー赤痢菌) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.7 Å | ||||||
データ登録者 | Tang, X.D. / Chang, S.H. / Luo, Q.H. / Zhang, Z.Y. / Qiao, W. / Xu, C.H. / Zhang, C.B. / Niu, Y. / Yang, W.X. / Wang, T. ...Tang, X.D. / Chang, S.H. / Luo, Q.H. / Zhang, Z.Y. / Qiao, W. / Xu, C.H. / Zhang, C.B. / Niu, Y. / Yang, W.X. / Wang, T. / Zhang, Z.B. / Zhu, X.F. / Dong, C.J. / Zhang, X. / Dong, H.H. | ||||||
引用 | ジャーナル: Nat Commun / 年: 2019 タイトル: Cryo-EM structures of lipopolysaccharide transporter LptBFGC in lipopolysaccharide or AMP-PNP-bound states reveal its transport mechanism. 著者: Xiaodi Tang / Shenghai Chang / Qinghua Luo / Zhengyu Zhang / Wen Qiao / Caihuang Xu / Changbin Zhang / Yang Niu / Wenxian Yang / Ting Wang / Zhibo Zhang / Xiaofeng Zhu / Xiawei Wei / ...著者: Xiaodi Tang / Shenghai Chang / Qinghua Luo / Zhengyu Zhang / Wen Qiao / Caihuang Xu / Changbin Zhang / Yang Niu / Wenxian Yang / Ting Wang / Zhibo Zhang / Xiaofeng Zhu / Xiawei Wei / Changjiang Dong / Xing Zhang / Haohao Dong / 要旨: Lipopolysaccharides (LPS) of Gram-negative bacteria are critical for the defence against cytotoxic substances and must be transported from the inner membrane (IM) to the outer membrane (OM) through ...Lipopolysaccharides (LPS) of Gram-negative bacteria are critical for the defence against cytotoxic substances and must be transported from the inner membrane (IM) to the outer membrane (OM) through a bridge formed by seven membrane proteins (LptBFGCADE). The IM component LptBFG powers the process through a yet unclarified mechanism. Here we report three high-resolution cryo-EM structures of LptBFG alone and complexed with LptC (LptBFGC), trapped in either the LPS- or AMP-PNP-bound state. The structures reveal conformational changes between these states and substrate binding with or without LptC. We identify two functional transmembrane arginine-containing loops interacting with the bound AMP-PNP and elucidate allosteric communications between the domains. AMP-PNP binding induces an inward rotation and shift of the transmembrane helices of LptFG and LptC to tighten the cavity, with the closure of two lateral gates, to eventually expel LPS into the bridge. Functional assays reveal the functionality of the LptF and LptG periplasmic domains. Our findings shed light on the LPS transport mechanism. #1: ジャーナル: Nat Commun / 年: 2019 タイトル: Cryo-EM structures of lipopolysaccharide transporter LptBFGC in lipopolysaccharide or AMP-PNP-bound states reveal its transport mechanism. 著者: Xiaodi Tang / Shenghai Chang / Qinghua Luo / Zhengyu Zhang / Wen Qiao / Caihuang Xu / Changbin Zhang / Yang Niu / Wenxian Yang / Ting Wang / Zhibo Zhang / Xiaofeng Zhu / Xiawei Wei / ...著者: Xiaodi Tang / Shenghai Chang / Qinghua Luo / Zhengyu Zhang / Wen Qiao / Caihuang Xu / Changbin Zhang / Yang Niu / Wenxian Yang / Ting Wang / Zhibo Zhang / Xiaofeng Zhu / Xiawei Wei / Changjiang Dong / Xing Zhang / Haohao Dong / 要旨: Lipopolysaccharides (LPS) of Gram-negative bacteria are critical for the defence against cytotoxic substances and must be transported from the inner membrane (IM) to the outer membrane (OM) through ...Lipopolysaccharides (LPS) of Gram-negative bacteria are critical for the defence against cytotoxic substances and must be transported from the inner membrane (IM) to the outer membrane (OM) through a bridge formed by seven membrane proteins (LptBFGCADE). The IM component LptBFG powers the process through a yet unclarified mechanism. Here we report three high-resolution cryo-EM structures of LptBFG alone and complexed with LptC (LptBFGC), trapped in either the LPS- or AMP-PNP-bound state. The structures reveal conformational changes between these states and substrate binding with or without LptC. We identify two functional transmembrane arginine-containing loops interacting with the bound AMP-PNP and elucidate allosteric communications between the domains. AMP-PNP binding induces an inward rotation and shift of the transmembrane helices of LptFG and LptC to tighten the cavity, with the closure of two lateral gates, to eventually expel LPS into the bridge. Functional assays reveal the functionality of the LptF and LptG periplasmic domains. Our findings shed light on the LPS transport mechanism. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6s8h.cif.gz | 196.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6s8h.ent.gz | 148 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6s8h.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 6s8h_validation.pdf.gz | 1.3 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 6s8h_full_validation.pdf.gz | 1.3 MB | 表示 | |
XML形式データ | 6s8h_validation.xml.gz | 35.5 KB | 表示 | |
CIF形式データ | 6s8h_validation.cif.gz | 51.2 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/s8/6s8h ftp://data.pdbj.org/pub/pdb/validation_reports/s8/6s8h | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
-Lipopolysaccharide ... , 2種, 3分子 ABF
#1: タンパク質 | 分子量: 26837.668 Da / 分子数: 2 / 由来タイプ: 組換発現 由来: (組換発現) Shigella flexneri (フレクスナー赤痢菌) 遺伝子: SGF_01136 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: E7T9E6, UniProt: P0A9V4*PLUS #2: タンパク質 | | 分子量: 40345.430 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) Shigella flexneri (フレクスナー赤痢菌) 遺伝子: lptF, SF4228, S4489 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P0AFA1 |
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-タンパク質 / 糖 , 2種, 3分子 G
#3: タンパク質 | 分子量: 39622.414 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) Shigella flexneri (フレクスナー赤痢菌) 遺伝子: yjgQ, S4488, CQA91_25110, NCTC9783_00309, SAMEA3710568_03584 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A2S4N3I3, UniProt: A0A0H2V3J7*PLUS |
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#7: 糖 |
-非ポリマー , 3種, 5分子
#4: 化合物 | #5: 化合物 | ChemComp-JSG / ( | |
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