MEMBRANE PROTEIN / MscS / Nanodisc / Mechanosensitive Channel of Small Conductance / Mechanosensitive / Channel
機能・相同性
機能・相同性情報
intracellular water homeostasis / mechanosensitive monoatomic ion channel activity / monoatomic ion transmembrane transport / protein homooligomerization / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能
Mechanosensitive ion channel MscS, archaea/bacteria type / Conserved TM helix / Mechanosensitive ion channel, conserved TM helix / : / : / Mechanosensitive ion channel MscS, C-terminal / Mechanosensitive ion channel, transmembrane helices 2/3 / Mechanosensitive ion channel MscS, conserved site / Uncharacterized protein family UPF0003 signature. / Mechanosensitive ion channel MscS, C-terminal ...Mechanosensitive ion channel MscS, archaea/bacteria type / Conserved TM helix / Mechanosensitive ion channel, conserved TM helix / : / : / Mechanosensitive ion channel MscS, C-terminal / Mechanosensitive ion channel, transmembrane helices 2/3 / Mechanosensitive ion channel MscS, conserved site / Uncharacterized protein family UPF0003 signature. / Mechanosensitive ion channel MscS, C-terminal / Mechanosensitive ion channel MscS, transmembrane-2 / Mechanosensitive ion channel MscS / Mechanosensitive ion channel, beta-domain / Mechanosensitive ion channel MscS, beta-domain superfamily / LSM domain superfamily 類似検索 - ドメイン・相同性
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
R01 GM133191
米国
引用
ジャーナル: Elife / 年: 2019 タイトル: Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS. 著者: Bharat Reddy / Navid Bavi / Allen Lu / Yeonwoo Park / Eduardo Perozo / 要旨: Prokaryotic mechanosensitive (MS) channels open by sensing the physical state of the membrane. As such, lipid-protein interactions represent the defining molecular process underlying ...Prokaryotic mechanosensitive (MS) channels open by sensing the physical state of the membrane. As such, lipid-protein interactions represent the defining molecular process underlying mechanotransduction. Here, we describe cryo-electron microscopy (cryo-EM) structures of the small-conductance mechanosensitive channel (MscS) in nanodiscs (ND). They reveal a novel membrane-anchoring fold that plays a significant role in channel activation and establish a new location for the lipid bilayer, shifted ~14 Å from previous consensus placements. Two types of lipid densities are explicitly observed. A phospholipid that 'hooks' the top of each TM2-TM3 hairpin and likely plays a role in force sensing, and a bundle of acyl chains occluding the permeation path above the L105 cuff. These observations reshape our understanding of force-from-lipids gating in MscS and highlight the key role of allosteric interactions between TM segments and phospholipids bound to key dynamic components of the channel.
名称: Mechanosensitive Channel of Small Conductance / タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT
分子量
単位: KILODALTONS/NANOMETER / 実験値: NO
由来(天然)
生物種: Escherichia coli (大腸菌)
由来(組換発現)
生物種: Escherichia coli (大腸菌)
緩衝液
pH: 7.4
試料
濃度: 2 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES
急速凍結
装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 295 K 詳細: 22C. Blot Force 3 for 3 seconds. Double application with a blotting between applications.