National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
R01 GM047795
米国
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)
S10 OD019995
米国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2019 タイトル: Capsid expansion of bacteriophage T5 revealed by high resolution cryoelectron microscopy. 著者: Alexis Huet / Robert L Duda / Pascale Boulanger / James F Conway / 要旨: The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a ...The large (90-nm) icosahedral capsid of bacteriophage T5 is composed of 775 copies of the major capsid protein (mcp) together with portal, protease, and decoration proteins. Its assembly is a regulated process that involves several intermediates, including a thick-walled round precursor prohead that expands as the viral DNA is packaged to yield a thin-walled and angular mature capsid. We investigated capsid maturation by comparing cryoelectron microscopy (cryo-EM) structures of the prohead, the empty expanded capsid both with and without decoration protein, and the virion capsid at a resolution of 3.8 Å for the latter. We detail the molecular structure of the mcp, its complex pattern of interactions, and their evolution during maturation. The bacteriophage T5 mcp is a variant of the canonical HK97-fold with a high level of plasticity that allows for the precise assembly of a giant macromolecule and the adaptability needed to interact with other proteins and the packaged DNA.
#200 - 2016年8月 正二十面体型ウイルスの準対称性 (Quasisymmetry in Icosahedral Viruses) 類似性 (3)
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集合体
登録構造単位
A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein E: Major capsid protein F: Major capsid protein G: Major capsid protein H: Major capsid protein I: Major capsid protein J: Major capsid protein K: Major capsid protein L: Major capsid protein M: Major capsid protein
A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein E: Major capsid protein F: Major capsid protein G: Major capsid protein H: Major capsid protein I: Major capsid protein J: Major capsid protein K: Major capsid protein L: Major capsid protein M: Major capsid protein
A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein E: Major capsid protein F: Major capsid protein G: Major capsid protein H: Major capsid protein I: Major capsid protein J: Major capsid protein K: Major capsid protein L: Major capsid protein M: Major capsid protein
x 5
icosahedral pentamer
2.14 MDa, 65 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
2,140,538
65
ポリマ-
2,140,538
65
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: Major capsid protein B: Major capsid protein C: Major capsid protein D: Major capsid protein E: Major capsid protein F: Major capsid protein G: Major capsid protein H: Major capsid protein I: Major capsid protein J: Major capsid protein K: Major capsid protein L: Major capsid protein M: Major capsid protein
x 6
icosahedral 23 hexamer
2.57 MDa, 78 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
2,568,646
78
ポリマ-
2,568,646
78
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
5
5
登録構造と同一(異なる座標系)
icosahedral asymmetric unit, std point frame
タイプ
名称
対称操作
数
transform to point frame
1
対称性
点対称性: (シェーンフリース記号: I (正20面体型対称))
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要素
#1: タンパク質
Majorcapsidprotein / Capsid protein pb8 / Major head protein