+Open data
-Basic information
Entry | Database: PDB / ID: 6lba | ||||||
---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of the AtMLKL2 tetramer | ||||||
Components | Protein kinase family protein | ||||||
Keywords | CYTOSOLIC PROTEIN / Plant MLKLs / Cryo-EM / Tetramer | ||||||
Function / homology | Function and homology information protein serine/threonine/tyrosine kinase activity / protein kinase activity / signal transduction / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.1 Å | ||||||
Authors | Lisa, M. / Huang, M. / Zhang, X. / Ryohei, T.N. / Leila, B.K. / Isabel, M.L.S. / Florence, J. / Viera, K. / Dmitry, L. / Jane, E.P. ...Lisa, M. / Huang, M. / Zhang, X. / Ryohei, T.N. / Leila, B.K. / Isabel, M.L.S. / Florence, J. / Viera, K. / Dmitry, L. / Jane, E.P. / James, M.M. / Kay, H. / Paul, S.L. / Chai, J. / Takaki, M. | ||||||
Citation | Journal: To Be Published Title: Cryo-EM structure of the AtMLKL3 tetramer Authors: Lisa, M. / Huang, M. / Zhang, X. / Ryohei, T.N. / Leila, B.K. / Isabel, M.L.S. / Florence, J. / Viera, K. / Dmitry, L. / Jane, E.P. / James, M.M. / Kay, H. / Paul, S.L. / Chai, J. / Takaki, M. | ||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6lba.cif.gz | 336.1 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6lba.ent.gz | 272.6 KB | Display | PDB format |
PDBx/mmJSON format | 6lba.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6lba_validation.pdf.gz | 824.8 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 6lba_full_validation.pdf.gz | 979.4 KB | Display | |
Data in XML | 6lba_validation.xml.gz | 78 KB | Display | |
Data in CIF | 6lba_validation.cif.gz | 109.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lb/6lba ftp://data.pdbj.org/pub/pdb/validation_reports/lb/6lba | HTTPS FTP |
-Related structure data
Related structure data | 0868MC 9954C 6ka4C C: citing same article (ref.) M: map data used to model this data |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
#1: Protein | Mass: 66682.531 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: MUF8.1, MUF8_1, At5g41730 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9FGG5 |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: 3D ARRAY / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Mixed lineage kinase domain-like (MLKL) protein 2 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 1.5625 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.15.1_3469: / Classification: refinement | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 1527009 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
|