National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM079238
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
T32 GM115327-Tan
米国
引用
ジャーナル: Cell Rep / 年: 2018 タイトル: tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis. 著者: Julia Flis / Mikael Holm / Emily J Rundlet / Justus Loerke / Tarek Hilal / Marylena Dabrowski / Jörg Bürger / Thorsten Mielke / Scott C Blanchard / Christian M T Spahn / Tatyana V Budkevich / 要旨: Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes ...Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes within both the ribosome and tRNA substrates, the orchestrated events that ensure the speed and fidelity of this critical aspect of the protein synthesis mechanism have not been fully elucidated. Here, we present three high-resolution structures of intermediates of translocation on the mammalian ribosome where, in contrast to bacteria, ribosomal complexes containing the translocase eEF2 and the complete tRNA⋅mRNA module are trapped by the non-hydrolyzable GTP analog GMPPNP. Consistent with the observed structures, single-molecule imaging revealed that GTP hydrolysis principally facilitates rate-limiting, final steps of translocation, which are required for factor dissociation and which are differentially regulated in bacterial and mammalian systems by the rates of deacyl-tRNA dissociation from the E site.
A2: 28S ribosomal RNA Bv: P/P-site-tRNA Bx: mRNA Bw: E/E-site-tRNA B1: 18S ribosomal RNA BD: ribosomal protein uS3 BF: ribosomal protein uS7 BK: ribosomal protein eS10 BM: ribosomal protein eS12 BP: ribosomal protein uS19 BQ: ribosomal protein uS9 BR: ribosomal protein eS17 BS: ribosomal protein uS13 BT: ribosomal protein eS19 BU: ribosomal protein uS10 BZ: ribosomal protein eS25 Bc: ribosomal protein eS28 Bd: ribosomal protein uS14 Bf: ribosomal protein eS31 Bg: ribosomal protein RACK 1 BA: ribosomal protein uS2 BB: ribosomal protein eS1 BC: ribosomal protein uS5 BE: ribosomal protein eS4 BG: ribosomal protein eS6 BH: ribosomal protein eS7 BI: ribosomal protein eS8 BJ: ribosomal protein uS4 BL: ribosomal protein uS17 BN: ribosomal protein uS15 BO: ribosomal protein uS11 BV: ribosomal protein eS21 BW: ribosomal protein uS8 BX: ribosomal protein uS12 BY: ribosomal protein eS24 Ba: ribosomal protein eS26 Bb: ribosomal protein eS27 Be: ribosomal protein eS30 A3: 5.8S ribosomal RNA A4: 5S ribosomal RNA AA: ribosomal protein uL2 AB: ribosomal protein uL3 AC: ribosomal protein uL4 AD: ribosomal protein uL18 AE: ribosomal protein eL6 AF: ribosomal protein uL30 AG: ribosomal protein eL8 AH: ribosomal protein uL6 AI: ribosomal protein uL16 AJ: ribosomal protein uL5 AL: ribosomal protein eL13 AM: ribosomal protein eL14 AN: ribosomal protein eL15 AO: ribosomal protein uL13 AP: ribosomal protein uL22 AQ: ribosomal protein eL18 AR: ribosomal protein eL19 AS: ribosomal protein eL20 AT: ribosomal protein eL21 AU: ribosomal protein eL22 AV: ribosomal protein uL14 AW: ribosomal protein eL24 AX: ribosomal protein uL23 AY: ribosomal protein uL24 AZ: ribosomal protein eL27 Aa: ribosomal protein uL15 Ab: ribosomal protein eL29 Ac: ribosomal protein eL30 Ad: ribosomal protein eL31 Ae: ribosomal protein eL32 Af: ribosomal protein eL33 Ag: ribosomal protein eL34 Ah: ribosomal protein uL29 Ai: ribosomal protein eL36 Aj: ribosomal protein eL37 Ak: ribosomal protein eL38 Al: ribosomal protein eL39 Am: ribosomal protein eL40 An: ribosomal protein eL41 Ao: ribosomal protein eL42 Ap: ribosomal protein eL43 At: ribosomal protein eL28 Au: ribosomal protein uL1 Aq: ribosomal protein uL11 AK: ribosomal protein uL10 Ct: eukaryotic elongation factor 2 (eEF2) ヘテロ分子
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基本情報
要素
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機能・相同性情報
Enterobacteria phage SP6 (ファージ)
Oryctolagus cuniculus (ウサギ)
Saccharomyces cerevisiae (パン酵母)
データ登録者
ドイツ, 
米国, 3件 
引用
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PDBj
集合体
分子量: 24.305 Da / 分子数: 327 / 由来タイプ: 合成 / 式: Mg
分子量: 65.409 Da / 分子数: 5 / 由来タイプ: 合成 / 式: Zn
分子量: 522.196 Da / 分子数: 1 / 由来タイプ: 合成 / 式: C10H17N6O13P3
試料調製
電子顕微鏡撮影
FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM
解析