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Yorodumi- PDB-5o6v: The cryo-EM structure of Tick-borne encephalitis virus complexed ... -
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-Basic information
Entry | Database: PDB / ID: 5o6v | ||||||||||||
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Title | The cryo-EM structure of Tick-borne encephalitis virus complexed with Fab fragment of neutralizing antibody 19/1786 | ||||||||||||
Components |
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Keywords | VIRUS / tick-borne encephalitis virus / Fab 19/1786 / cryo-EM | ||||||||||||
Function / homology | Function and homology information flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / viral capsid / double-stranded RNA binding / nucleoside-triphosphate phosphatase / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / clathrin-dependent endocytosis of virus by host cell / mRNA (nucleoside-2'-O-)-methyltransferase activity ...flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / viral capsid / double-stranded RNA binding / nucleoside-triphosphate phosphatase / mRNA (guanine-N7)-methyltransferase / methyltransferase cap1 / clathrin-dependent endocytosis of virus by host cell / mRNA (nucleoside-2'-O-)-methyltransferase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / RNA helicase activity / host cell perinuclear region of cytoplasm / host cell endoplasmic reticulum membrane / protein dimerization activity / symbiont-mediated suppression of host innate immune response / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / RNA helicase / induction by virus of host autophagy / serine-type endopeptidase activity / RNA-directed RNA polymerase / viral RNA genome replication / virus-mediated perturbation of host defense response / RNA-dependent RNA polymerase activity / fusion of virus membrane with host endosome membrane / viral envelope / host cell nucleus / virion attachment to host cell / structural molecule activity / virion membrane / ATP hydrolysis activity / proteolysis / extracellular region / ATP binding / membrane / metal ion binding Similarity search - Function | ||||||||||||
Biological species | Tick-borne encephalitis virus Mus musculus (house mouse) | ||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.9 Å | ||||||||||||
Authors | Fuzik, T. / Plevka, P. | ||||||||||||
Funding support | Germany, Czech Republic, 3items
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Citation | Journal: Nat Commun / Year: 2018 Title: Structure of tick-borne encephalitis virus and its neutralization by a monoclonal antibody. Authors: Tibor Füzik / Petra Formanová / Daniel Růžek / Kentaro Yoshii / Matthias Niedrig / Pavel Plevka / Abstract: Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. ...Tick-borne encephalitis virus (TBEV) causes 13,000 cases of human meningitis and encephalitis annually. However, the structure of the TBEV virion and its interactions with antibodies are unknown. Here, we present cryo-EM structures of the native TBEV virion and its complex with Fab fragments of neutralizing antibody 19/1786. Flavivirus genome delivery depends on membrane fusion that is triggered at low pH. The virion structure indicates that the repulsive interactions of histidine side chains, which become protonated at low pH, may contribute to the disruption of heterotetramers of the TBEV envelope and membrane proteins and induce detachment of the envelope protein ectodomains from the virus membrane. The Fab fragments bind to 120 out of the 180 envelope glycoproteins of the TBEV virion. Unlike most of the previously studied flavivirus-neutralizing antibodies, the Fab fragments do not lock the E-proteins in the native-like arrangement, but interfere with the process of virus-induced membrane fusion. | ||||||||||||
History |
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-Structure visualization
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Structure viewer | Molecule: MolmilJmol/JSmol |
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PDBx/mmCIF format | 5o6v.cif.gz | 479.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5o6v.ent.gz | 402.1 KB | Display | PDB format |
PDBx/mmJSON format | 5o6v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5o6v_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 5o6v_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 5o6v_validation.xml.gz | 75.3 KB | Display | |
Data in CIF | 5o6v_validation.cif.gz | 115.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/o6/5o6v ftp://data.pdbj.org/pub/pdb/validation_reports/o6/5o6v | HTTPS FTP |
-Related structure data
Related structure data | 3754MC 3752C 3755C 5o6aC C: citing same article (ref.) M: map data used to model this data |
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Similar structure data |
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Noncrystallographic symmetry (NCS) | NCS oper:
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