+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 4c2i | |||||||||
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タイトル | Cryo-EM structure of Dengue virus serotype 1 complexed with Fab fragments of human antibody 1F4 | |||||||||
要素 |
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キーワード | VIRUS / E PROTEINS / NEUTRALIZATION | |||||||||
機能・相同性 | 機能・相同性情報 symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / host cell mitochondrion / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / ribonucleoside triphosphate phosphatase activity / channel activity / double-stranded RNA binding / viral capsid / monoatomic ion transmembrane transport / clathrin-dependent endocytosis of virus by host cell ...symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / host cell mitochondrion / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / ribonucleoside triphosphate phosphatase activity / channel activity / double-stranded RNA binding / viral capsid / monoatomic ion transmembrane transport / clathrin-dependent endocytosis of virus by host cell / mRNA (nucleoside-2'-O-)-methyltransferase activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / RNA helicase activity / protein dimerization activity / host cell endoplasmic reticulum membrane / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / induction by virus of host autophagy / viral RNA genome replication / serine-type endopeptidase activity / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / viral envelope / host cell nucleus / virion attachment to host cell / structural molecule activity / virion membrane / proteolysis / extracellular region / ATP binding / membrane / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | DENGUE VIRUS 1 (デング熱ウイルス) HOMO SAPIENS (ヒト) | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 6 Å | |||||||||
Model type details | CA ATOMS ONLY, CHAIN A, C, E, B, D, F, H, M, L, N | |||||||||
データ登録者 | Fibriansah, G. / Tan, J.L. / de Alwis, R. / Smith, S.A. / Ng, T.-S. / Kostyuchenko, V.A. / Ibarra, K.D. / Harris, E. / de Silva, A. / Crowe Junior, J.E. / Lok, S.-M. | |||||||||
引用 | ジャーナル: EMBO Mol Med / 年: 2014 タイトル: A potent anti-dengue human antibody preferentially recognizes the conformation of E protein monomers assembled on the virus surface. 著者: Guntur Fibriansah / Joanne L Tan / Scott A Smith / Adamberage R de Alwis / Thiam-Seng Ng / Victor A Kostyuchenko / Kristie D Ibarra / Jiaqi Wang / Eva Harris / Aravinda de Silva / James E Crowe / Shee-Mei Lok / 要旨: Dengue virus (DENV), which consists of four serotypes (DENV1-4), infects over 400 million people annually. Previous studies have indicated most human monoclonal antibodies (HMAbs) from dengue ...Dengue virus (DENV), which consists of four serotypes (DENV1-4), infects over 400 million people annually. Previous studies have indicated most human monoclonal antibodies (HMAbs) from dengue patients are cross-reactive and poorly neutralizing. Rare neutralizing HMAbs are usually serotype-specific and bind to quaternary structure-dependent epitopes. We determined the structure of DENV1 complexed with Fab fragments of a highly potent HMAb 1F4 to 6 Å resolution by cryo-EM. Although HMAb 1F4 appeared to bind to virus and not E proteins in ELISAs in the previous study, our structure showed that the epitope is located within an envelope (E) protein monomer, and not across neighboring E proteins. The Fab molecules bind to domain I (DI), and DI-DII hinge of the E protein. We also showed that HMAb 1F4 can neutralize DENV at different stages of viral entry in a cell type and receptor dependent manner. The structure reveals the mechanism by which this potent and specific antibody blocks viral infection. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 4c2i.cif.gz | 96.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb4c2i.ent.gz | 59.1 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 4c2i.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 4c2i_validation.pdf.gz | 1.5 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 4c2i_full_validation.pdf.gz | 1.5 MB | 表示 | |
XML形式データ | 4c2i_validation.xml.gz | 38.5 KB | 表示 | |
CIF形式データ | 4c2i_validation.cif.gz | 58.1 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/c2/4c2i ftp://data.pdbj.org/pub/pdb/validation_reports/c2/4c2i | HTTPS FTP |
-関連構造データ
-リンク
-集合体
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対称性 | 点対称性: (シェーンフリース記号: I (正20面体型対称)) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
非結晶学的対称性 (NCS) | NCS oper:
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