ジャーナル: J Virol / 年: 2013 タイトル: Immature and mature dengue serotype 1 virus structures provide insight into the maturation process. 著者: Victor A Kostyuchenko / Qian Zhang / Joanne L Tan / Thiam-Seng Ng / Shee-Mei Lok / 要旨: Dengue virus is a major human pathogen that has four serotypes (DENV1 to -4). Here we report the cryoelectron microscopy (cryo-EM) structures of immature and mature DENV1 at 6- and 4.5-Å resolution, ...Dengue virus is a major human pathogen that has four serotypes (DENV1 to -4). Here we report the cryoelectron microscopy (cryo-EM) structures of immature and mature DENV1 at 6- and 4.5-Å resolution, respectively. The subnanometer-resolution maps allow accurate placement of all of the surface proteins. Although the immature and mature viruses showed vastly different surface protein organizations, the envelope protein transmembrane (E-TM) regions remain in similar positions. The pivotal role of the E-TM regions leads to the identification of the start and end positions of all surface proteins during maturation.
履歴
登録
2012年6月28日
登録サイト: PDBE / 処理サイト: PDBE
改定 1.0
2013年6月5日
Provider: repository / タイプ: Initial release
改定 1.1
2013年6月26日
Group: Database references
改定 1.2
2017年4月19日
Group: Other
改定 1.3
2017年8月30日
Group: Data collection / カテゴリ: em_software Item: _em_software.fitting_id / _em_software.image_processing_id
A: DENGUE VIRUS 1 E PROTEIN B: DENGUE VIRUS 1 E PROTEIN C: DENGUE VIRUS 1 E PROTEIN D: DENGUE VIRUS 1 PRM PROTEIN E: DENGUE VIRUS 1 PRM PROTEIN F: DENGUE VIRUS 1 PRM PROTEIN
A: DENGUE VIRUS 1 E PROTEIN B: DENGUE VIRUS 1 E PROTEIN C: DENGUE VIRUS 1 E PROTEIN D: DENGUE VIRUS 1 PRM PROTEIN E: DENGUE VIRUS 1 PRM PROTEIN F: DENGUE VIRUS 1 PRM PROTEIN
A: DENGUE VIRUS 1 E PROTEIN B: DENGUE VIRUS 1 E PROTEIN C: DENGUE VIRUS 1 E PROTEIN D: DENGUE VIRUS 1 PRM PROTEIN E: DENGUE VIRUS 1 PRM PROTEIN F: DENGUE VIRUS 1 PRM PROTEIN
x 5
icosahedral pentamer
1.08 MDa, 30 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
1,084,801
30
ポリマ-
1,084,801
30
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: DENGUE VIRUS 1 E PROTEIN B: DENGUE VIRUS 1 E PROTEIN C: DENGUE VIRUS 1 E PROTEIN D: DENGUE VIRUS 1 PRM PROTEIN E: DENGUE VIRUS 1 PRM PROTEIN F: DENGUE VIRUS 1 PRM PROTEIN