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- PDB-28nt: Cryo-EM structure of the 70S ribosome from Francisella tularensis -

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Basic information

Entry
Database: PDB / ID: 28nt
TitleCryo-EM structure of the 70S ribosome from Francisella tularensis
Components
  • (Large ribosomal subunit protein ...) x 29
  • (Small ribosomal subunit protein ...) x 17
  • 16S ribosomal RNA
  • 23S ribosomal RNA
  • 5S ribosomal RNA
KeywordsRIBOSOME / cryo-EM / 70S / Francisella / F. tularensis
Function / homology
Function and homology information


assembly of large subunit precursor of preribosome / large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly ...assembly of large subunit precursor of preribosome / large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / metal ion binding / cytoplasm / cytosol
Similarity search - Function
Ribosomal protein L25, long-form / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily ...Ribosomal protein L25, long-form / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein S21 / Ribosomal protein L9 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L21 signature. / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / : / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L28/L24 superfamily / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / : / Ribosomal protein S13, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S4, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S5, bacterial-type / 30S ribosomal protein S17 / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal protein S18, conserved site / Ribosomal L28 family / Ribosomal protein S18 signature. / Ribosomal protein L33 superfamily / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein L16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein L20 / Ribosomal protein S12, bacterial-type
Similarity search - Domain/homology
: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 ...: / : / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein bS21B / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL29
Similarity search - Component
Biological speciesFrancisella tularensis (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.95 Å
AuthorsSilhan, J. / Klima, M. / Boura, E.
Funding support1items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Cryo-EM structure of the 70S ribosome from Francisella tularensis
Authors: Klima, M. / Silhan, J. / Boura, E.
History
DepositionFeb 10, 2026Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 6, 2026Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0May 6, 2026Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 23S ribosomal RNA
B: 5S ribosomal RNA
C: Large ribosomal subunit protein uL2
D: Large ribosomal subunit protein uL3
E: Large ribosomal subunit protein uL4
F: Large ribosomal subunit protein uL5
G: Large ribosomal subunit protein uL6
H: Large ribosomal subunit protein bL9
I: Large ribosomal subunit protein uL13
J: Large ribosomal subunit protein uL14
K: Large ribosomal subunit protein uL15
L: Large ribosomal subunit protein uL16
M: Large ribosomal subunit protein bL17
N: Large ribosomal subunit protein uL18
O: Large ribosomal subunit protein bL19
P: Large ribosomal subunit protein bL20
Q: Large ribosomal subunit protein bL21
R: Large ribosomal subunit protein uL22
S: Large ribosomal subunit protein uL23
T: Large ribosomal subunit protein uL24
U: Large ribosomal subunit protein bL25
V: Large ribosomal subunit protein bL27
W: Large ribosomal subunit protein bL28
X: Large ribosomal subunit protein uL29
Y: Large ribosomal subunit protein uL30
Z: Large ribosomal subunit protein bL31
0: Large ribosomal subunit protein bL32
1: Large ribosomal subunit protein bL33
2: Large ribosomal subunit protein bL34
3: Large ribosomal subunit protein bL35
4: Large ribosomal subunit protein bL36
a: 16S ribosomal RNA
b: Small ribosomal subunit protein uS2
d: Small ribosomal subunit protein uS4
e: Small ribosomal subunit protein uS5
f: Small ribosomal subunit protein bS6
g: Small ribosomal subunit protein uS7
h: Small ribosomal subunit protein uS8
i: Small ribosomal subunit protein uS9
k: Small ribosomal subunit protein uS11
l: Small ribosomal subunit protein uS12
m: Small ribosomal subunit protein uS13
o: Small ribosomal subunit protein uS15
p: Small ribosomal subunit protein bS16
q: Small ribosomal subunit protein uS17
r: Small ribosomal subunit protein bS18
s: Small ribosomal subunit protein uS19
t: Small ribosomal subunit protein bS20
u: Small ribosomal subunit protein bS21B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,079,982203
Polymers2,076,19849
Non-polymers3,784154
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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RNA chain , 3 types, 3 molecules ABa

#1: RNA chain 23S ribosomal RNA


Mass: 935337.812 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: GenBank: 1953470990
#2: RNA chain 5S ribosomal RNA


Mass: 36996.062 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: GenBank: 1919226889
#32: RNA chain 16S ribosomal RNA


Mass: 495164.062 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: GenBank: 1919226889

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Large ribosomal subunit protein ... , 29 types, 29 molecules CDEFGHIJKLMNOPQRSTUVWXYZ01234

#3: Protein Large ribosomal subunit protein uL2 / 50S ribosomal protein L2


Mass: 30469.639 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9S9
#4: Protein Large ribosomal subunit protein uL3 / 50S ribosomal protein L3


Mass: 22339.348 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JC0
#5: Protein Large ribosomal subunit protein uL4 / 50S ribosomal protein L4


Mass: 22587.189 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JB9
#6: Protein Large ribosomal subunit protein uL5 / 50S ribosomal protein L5


Mass: 20028.385 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9T7
#7: Protein Large ribosomal subunit protein uL6 / 50S ribosomal protein L6


Mass: 19106.051 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9U0
#8: Protein Large ribosomal subunit protein bL9 / 50S ribosomal protein L9


Mass: 16107.442 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NC56
#9: Protein Large ribosomal subunit protein uL13 / 50S ribosomal protein L13


Mass: 15969.677 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q2A332
#10: Protein Large ribosomal subunit protein uL14 / 50S ribosomal protein L14


Mass: 13259.521 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JB0
#11: Protein Large ribosomal subunit protein uL15 / 50S ribosomal protein L15


Mass: 15126.674 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9U4
#12: Protein Large ribosomal subunit protein uL16


Mass: 15742.542 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A0A0B3VDL9
#13: Protein Large ribosomal subunit protein bL17 / 50S ribosomal protein L17


Mass: 16818.420 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9V0
#14: Protein Large ribosomal subunit protein uL18 / 50S ribosomal protein L18


Mass: 13064.138 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JA4
#15: Protein Large ribosomal subunit protein bL19 / 50S ribosomal protein L19


Mass: 13334.611 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NEB0
#16: Protein Large ribosomal subunit protein bL20 / 50S ribosomal protein L20


Mass: 13378.632 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NDB3
#17: Protein Large ribosomal subunit protein bL21 / 50S ribosomal protein L21


Mass: 11585.680 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NDG2
#18: Protein Large ribosomal subunit protein uL22 / 50S ribosomal protein L22


Mass: 12223.330 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JB5
#19: Protein Large ribosomal subunit protein uL23 / 50S ribosomal protein L23


Mass: 11156.856 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9S8
#20: Protein Large ribosomal subunit protein uL24 / 50S ribosomal protein L24


Mass: 11498.368 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9T6
#21: Protein Large ribosomal subunit protein bL25 / 50S ribosomal protein L25


Mass: 10925.688 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NBX3
#22: Protein Large ribosomal subunit protein bL27 / 50S ribosomal protein L27


Mass: 9055.426 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NDG1
#23: Protein Large ribosomal subunit protein bL28 / 50S ribosomal protein L28


Mass: 8960.500 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14G26
#24: Protein Large ribosomal subunit protein uL29 / 50S ribosomal protein L29


Mass: 7819.249 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q2A5G2
#25: Protein Large ribosomal subunit protein uL30 / 50S ribosomal protein L30


Mass: 6884.125 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JA2
#26: Protein Large ribosomal subunit protein bL31 / 50S ribosomal protein L31


Mass: 8284.602 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q2A2S8
#27: Protein Large ribosomal subunit protein bL32 / 50S ribosomal protein L32


Mass: 6895.780 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NCH9
#28: Protein Large ribosomal subunit protein bL33 / 50S ribosomal protein L33


Mass: 6158.435 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14G25
#29: Protein/peptide Large ribosomal subunit protein bL34 / 50S ribosomal protein L34


Mass: 5198.318 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JK5
#30: Protein Large ribosomal subunit protein bL35 / 50S ribosomal protein L35


Mass: 7390.867 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14I18
#31: Protein/peptide Large ribosomal subunit protein bL36 / 50S ribosomal protein L36


Mass: 4437.495 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14J99

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Small ribosomal subunit protein ... , 17 types, 17 molecules bdefghiklmopqrstu

#33: Protein Small ribosomal subunit protein uS2 / 30S ribosomal protein S2


Mass: 24729.816 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9R4
#34: Protein Small ribosomal subunit protein uS4 / 30S ribosomal protein S4


Mass: 23148.707 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14J96
#35: Protein Small ribosomal subunit protein uS5 / 30S ribosomal protein S5


Mass: 17554.285 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q2A5F3
#36: Protein Small ribosomal subunit protein bS6 / 30S ribosomal protein S6


Mass: 13075.109 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14HF1
#37: Protein Small ribosomal subunit protein uS7 / 30S ribosomal protein S7


Mass: 17844.732 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JC3
#38: Protein Small ribosomal subunit protein uS8 / 30S ribosomal protein S8


Mass: 14431.798 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9T9
#39: Protein Small ribosomal subunit protein uS9


Mass: 14764.072 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A0A0E2ZLL8
#40: Protein Small ribosomal subunit protein uS11 / 30S ribosomal protein S11


Mass: 13786.841 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9U7
#41: Protein Small ribosomal subunit protein uS12 / 30S ribosomal protein S12


Mass: 13846.171 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JC4
#42: Protein Small ribosomal subunit protein uS13 / 30S ribosomal protein S13


Mass: 13406.733 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14J98
#43: Protein Small ribosomal subunit protein uS15 / 30S ribosomal protein S15


Mass: 10383.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NDP4
#44: Protein Small ribosomal subunit protein bS16 / 30S ribosomal protein S16


Mass: 9091.747 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NEB3
#45: Protein Small ribosomal subunit protein uS17 / 30S ribosomal protein S17


Mass: 9874.555 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9T4
#46: Protein Small ribosomal subunit protein bS18 / 30S ribosomal protein S18


Mass: 8395.854 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7NC55
#47: Protein Small ribosomal subunit protein uS19 / 30S ribosomal protein S19


Mass: 10523.450 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q14JB6
#48: Protein Small ribosomal subunit protein bS20 / 30S ribosomal protein S20


Mass: 10173.040 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: A7N9A3
#49: Protein Small ribosomal subunit protein bS21B / 30S ribosomal protein S21 2


Mass: 7863.384 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Francisella tularensis (bacteria) / References: UniProt: Q2A3F5

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Non-polymers , 2 types, 154 molecules

#50: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 153 / Source method: obtained synthetically / Formula: Mg
#51: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn

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Details

Has ligand of interestN
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 70S ribosome from Francisella tularensis / Type: RIBOSOME / Entity ID: #1-#49 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Francisella tularensis (bacteria) / Strain: FSC200
Buffer solutionpH: 7.5
Details: HEPES 7.5 20 mM magnesium acetate 10 mM potassium chloride 7 mM mercaptoethanol
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R2/1
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 800 nm
Image recordingElectron dose: 40 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

EM software
IDNameVersionCategory
1cryoSPARCv4.6.2particle selection
13cryoSPARCv4.6.23D reconstruction
CTF correctionType: PHASE FLIPPING ONLY
3D reconstructionResolution: 2.95 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 33316 / Symmetry type: POINT
RefinementCross valid method: NONE

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