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- EMDB-55615: Cryo-EM structure of the 70S ribosome from Francisella tularensis... -

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Basic information

Entry
Database: EMDB / ID: EMD-55615
TitleCryo-EM structure of the 70S ribosome from Francisella tularensis bound to antibiotics chloramphenicol and gentamicin
Map dataCryoEM map of 70S Francisela tularensis ribosome with Cm and GEN bound.
Sample
  • Complex: 70S Ribosome from Francisella tularensis, combined with antibiotics
    • RNA: x 3 types
    • Protein or peptide: x 50 types
  • Ligand: x 4 types
KeywordsRibosome-associated inhibitor A RaiA Chloramphenicol Gentamicin / RIBOSOME
Function / homology
Function and homology information


negative regulation of translational elongation / ribosomal small subunit binding / assembly of large subunit precursor of preribosome / large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding ...negative regulation of translational elongation / ribosomal small subunit binding / assembly of large subunit precursor of preribosome / large ribosomal subunit / ribosomal small subunit assembly / transferase activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / metal ion binding / cytoplasm / cytosol
Similarity search - Function
: / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A ...: / Ribosome hibernation promoting factor/RaiA / Ribosome hibernation promotion factor-like / Sigma 54 modulation protein / S30EA ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein S21 / Ribosomal protein L9 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L21 signature. / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / : / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L28/L24 superfamily / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S3, bacterial-type / : / Ribosomal protein S13, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S4, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S5, bacterial-type / 30S ribosomal protein S17 / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal protein S18, conserved site / Ribosomal L28 family / Ribosomal protein S18 signature. / Ribosomal protein L33 superfamily / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein L16
Similarity search - Domain/homology
Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS9 / Ribosome hibernation promoting factor / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL24 ...Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS9 / Ribosome hibernation promoting factor / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein uL14 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein bS21B / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL29
Similarity search - Component
Biological speciesFrancisella (bacteria) / Francisella tularensis (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.4 Å
AuthorsSilhan J / Klima M / Boura E
Funding support Czech Republic, 1 items
OrganizationGrant numberCountry
Czech Science Foundation Czech Republic
CitationJournal: To Be Published
Title: Cryo-EM structure of the 70S ribosome from Francisella tularensis bound to antibiotics chloramphenicol and gentamicin
Authors: Silhan J / Klima M / Boura E
History
DepositionNov 7, 2025-
Header (metadata) releaseMay 6, 2026-
Map releaseMay 6, 2026-
UpdateMay 6, 2026-
Current statusMay 6, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_55615.png

Downloads & links

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Map

FileDownload / File: emd_55615.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryoEM map of 70S Francisela tularensis ribosome with Cm and GEN bound.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.76 Å/pix.
x 600 pix.
= 456. Å		0.76 Å/pix.
x 600 pix.
= 456. Å		0.76 Å/pix.
x 600 pix.
= 456. Å

Surface

Projections

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.76 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.05
Minimum - Maximum-0.13347152 - 0.37247363
Average (Standard dev.)0.00016157904 (±0.010017487)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions600600600
Spacing600600600
CellA=B=C: 456.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_55615_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_55615_half_map_2.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 70S Ribosome from Francisella tularensis, combined with antibiotics

EntireName: 70S Ribosome from Francisella tularensis, combined with antibiotics
Components
  • Complex: 70S Ribosome from Francisella tularensis, combined with antibiotics
    • RNA: 23S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein uL5
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: Large ribosomal subunit protein bL9
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: Large ribosomal subunit protein uL14
    • Protein or peptide: Large ribosomal subunit protein uL15
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein bL17
    • Protein or peptide: Large ribosomal subunit protein uL18
    • Protein or peptide: Large ribosomal subunit protein bL19
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: Large ribosomal subunit protein bL21
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein bL25
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • Protein or peptide: Large ribosomal subunit protein bL31
    • Protein or peptide: Large ribosomal subunit protein bL32
    • Protein or peptide: Large ribosomal subunit protein bL33
    • Protein or peptide: Large ribosomal subunit protein bL34
    • Protein or peptide: Large ribosomal subunit protein bL35
    • Protein or peptide: Large ribosomal subunit protein bL36
    • RNA: 16S ribosomal RNA
    • Protein or peptide: Small ribosomal subunit protein uS2
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: Small ribosomal subunit protein uS4
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Small ribosomal subunit protein bS6
    • Protein or peptide: Small ribosomal subunit protein uS7
    • Protein or peptide: Small ribosomal subunit protein uS8
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: Small ribosomal subunit protein uS10
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: Small ribosomal subunit protein uS14
    • Protein or peptide: Small ribosomal subunit protein uS15
    • Protein or peptide: Small ribosomal subunit protein bS16
    • Protein or peptide: Small ribosomal subunit protein uS17
    • Protein or peptide: Small ribosomal subunit protein bS18
    • Protein or peptide: Small ribosomal subunit protein uS19
    • Protein or peptide: Small ribosomal subunit protein bS20
    • Protein or peptide: Small ribosomal subunit protein bS21B
    • Protein or peptide: Ribosome hibernation promoting factor
  • Ligand: (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL
  • Ligand: MAGNESIUM ION
  • Ligand: CHLORAMPHENICOL
  • Ligand: ZINC ION

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Supramolecule #1: 70S Ribosome from Francisella tularensis, combined with antibiotics

SupramoleculeName: 70S Ribosome from Francisella tularensis, combined with antibiotics
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
Source (natural)Organism: Francisella (bacteria)

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Macromolecule #1: 23S ribosomal RNA

MacromoleculeName: 23S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 935.337812 KDa
SequenceString: GACUUAAGAA AAUAAGUGCA AGCGGUGGAU GCCUUGGCAU UCAGAGGCGA UGAAGGACGU GAUAAUCUGC GAUAAGCUUC GGUUAGCUG GUAAAUGAGC UAUGACCCGG AGAUUUCCGA AUGGGGGAAC CCACCUGACA CAAGUUGGGU ACUCACUCGA U AUAGAGUG ...String:
GACUUAAGAA AAUAAGUGCA AGCGGUGGAU GCCUUGGCAU UCAGAGGCGA UGAAGGACGU GAUAAUCUGC GAUAAGCUUC GGUUAGCUG GUAAAUGAGC UAUGACCCGG AGAUUUCCGA AUGGGGGAAC CCACCUGACA CAAGUUGGGU ACUCACUCGA U AUAGAGUG UAGAGCGAAC GAGGGGAACU GAAACAUCUA AGUACCCUUA GGAAGAGAAA UCAAUUGAGA UUCCCGUAGU AG UGGCGAG CGAAGUGGGA AGAGCCUGGU AUGAUUUAGC UGUAAUUAUA GUAGAACAAG UUGGGAAGCU UGACGAUAGA GGG UGAUAG UCCCGUAUAC GAAAUAAUCA CAGUGGAACU AAGCAUACGA ACAAGUAGGA CGGGGCACGU GGAACCUUGU CUGA AUAUG GGGGGACCAU CCUCCAAGGC UAAAUACUCC UGAAUGACCG AUAGUGGACU AGUACCGUGA GGGAAAGGUG AAAAG AACC CUUAUAAAGG GAGUGAAAUA GAAUCUGAAA CCGCUUGCAU ACAAGCAGUA GGAGCAUGAU UUAGUCAUGU GACUGC GUA CCUUUUGUAU AAUGGGUCAG CGAGUUAUUU UUAGUGGCGA GGAUAACUGA AUAAGGGAUC CGUAGCGAAA GCGAGUU UU AAUAGGGCGA CUAGUCGCUA GGAGUAGACC CGAAACCGGC GCGAUCUAUC CAUGGCCAGG UUGAAGAUUA GGUAGUAC U AAUUGGAGGA CCGAACCCAA UACUGUUGCA AAAGUAUGGG AUGAGCUGUG GAUCGGAGUG AAAGGCUAAU CAAGCACGG AGAUAGCUGG UUCUCCCCGA AAACUAUUUA GGUAGUGCCU CGUGUAUAAC UCAUUGGGGU AAAGCACUGU UUCGACAAUG GGGGUUUUA CGACCUUACU GACUCGAUGC AAACUCAGAA UACGAUGAAG UUCGAUCACG GGAGACACAC UGCGGGUGCU A AGGUCCGC AGUGGAAAGG GAAACAGCCC AGACCGCCAA CUAAGGUCCC AAAGUCAUAG CUAAGUGGGA AACGAAGUGG GA AGGCCCA GACAGCCAGG AGGUUGGCUU AGAAGCAGCC ACCCUUUAAA GAAAGCGUAA UAGCUCACUG GUCGAGUCGG CCU GCACGU AAGAUUUAAC GGGGCUAAGC UAUGCACUGA AGUUGCGGAA UAUAUUUAGU AUAUUGGUAG GGGAGCGUUC UGUA AGCCG AUGAAGGUGA AUUGAGAAGU UUGCUGGAGG UAUCAGAAGU GCGAAUGCUG ACAUGAGUAA CGUAAAAUAA GUGAG AUUC UUAUUGGCCG AAAACCCAAG GGUUCCUACG CAAUGUUAAU CAACGUAGGG UAAGCCGGCC CCUAAGGCGU AGCUGA AGA GUGAAGUCGA UGGGAAACAG GUUAAUAUUC CUGUGCUGCU UAUAUGAACG AAGGAGGGAC GGAGAAGGUU AGGUAGG CC UGGCGAAUGG UUGUCCAGGU GAAAGUAUGU AGGUAGGAAU GCUAGGCAAA UCCGGUGUUC UGUUGAUCUG AGAUACGA G ACGAAGUUAA ACUUGUUUGA CGAAGCUAUU GAUACCAUGC UUCCAGGAAA AGCUUCUAAG UAUAUUGUAU AAGUGACCG UACUGUAAAC CGACACUGGU GGGUAGGUAG AGAAUACUAA GGCUAUGAGA UAACUCUGGU GAAGGAACUA GGCAAAAUGA CACCGUAAC UUUGGAAGAA GGUGUGCCCU UGAUGGUGAU GAGACUUGCU CUUUGAGCUG UUGGGGGUUG CAGAUACCAG G UGGCUGCG ACUGUUUAUC AAAAACACAG CACUCUGCGA AAUCGUAAGA UGAAGUAUAG GGUGUGACGC CUGCCCGGUG CU GGAAGGU UAAUUGAAGG GGUUAGCGCA AGCGAAGCUC UGGAUCGAAG CCCCAGUAAA CGGCGGCCGU AACUAUAACG GUC CUAAGG UAGCGAAAUU CCUUGUCGGG UAAGUUCCGA CCUGCACGAA UGGCGUAACG AUGGCCACAC UGUCUCCACC AGAG ACUCA GUGAAAUUGA AAUCGCUGUG AAGAUGCAGU GUACCCGCGG UUAGACGGAC AGACCCCGUG AACCUUUACU AUAGC UUUG CACUGGACUU UGAAUAUUUA UGUGUAGGAU AGGUGGGAGA CCAUGAAGCA GCUACGCCAG UAGUUGUGGA GUCGUC CUU GAAAUACCAC CCUUGAAUAU UUGAAGUUCU AACUCAGGAG AGAUUCGAGG ACAGUGUAUG GUGGGUAGUU UGACUGG GG CGGUCUCCUC CUAAAGAGUA ACGGAGGAGU ACGAAGGUGC ACUCGGUACG GUCGGAAAUC GUGCCAAGAG UAUAAAGG C AAAAGUGCGC UUGACUGCGA GAGUGACGGC UCGAGCAGGU ACGAAAGUAG GUCUUAGUGA UCCGGUGGUC CCGAAUGGA AGGGUCAUCG CUCAACGGAU AAAAGGUACU CCG(2MG)GGAUAA CAGGCUAAUU CCUCCCAAGA GUUCAUAUCG ACGGAG GAG UUUGGCACCU CGAUGUCGGC UCAUCACAUC CUGGGGCUGA AGUAGGUCCC AAGGGUAUGG CUGUUCGCCA UUUAAAG UG GUACGCGAGC UGGGUUCAGA ACGUCGUGAG ACAGUUCGGU CCCUAUCUGC CGUGGGCGUU AGAGAUUUGA GAAGAGUU G CUCCUAGUAC GAGAGGACCG GAGUGAACGA ACCACUGGCG UUCCGGUUGU UUCGCCAGAA GCAUUGCCGG GUAGCUACG UUUGGACGGG AUAAACGCUG AAAGCAUCUA AGCGUGAAGC CUCCUUCAAG AUUAGAUCUC UCUGAUGAAA AUCAGUAAGG AACGUUGGA GACUACGACG UUGAUAGGCU GGGUGUGGAA GUACAGCAAU GUAUGAAGCU UACCAGUACU AAUGAUCCGA G AGACUUAA GU

GENBANK: GENBANK: CP066295.1

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Macromolecule #2: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 36.996062 KDa
SequenceString:
AUUGACGAUG AUAGCAGUCU GGAACCACCU GAUCCCAUUC CGAACUCAGA AGUGAAACGG UCUCACGCCA AUGAUAGUCU GGCACUGCC CAGGUGAAAG UAGGUAAUCG UCAAAC

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Macromolecule #32: 16S ribosomal RNA

MacromoleculeName: 16S ribosomal RNA / type: rna / ID: 32 / Number of copies: 1
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 495.164062 KDa
SequenceString: UAAACUGAAG AGUUUGAUCC UGGCUCAGAU UGAACGCUGG UGGCAUGCUU AACACAUGCA AGUCGAACGG UAACAGGUCU UAGGAUGCU GACGAGUGGC GGACGGGUGA GUAACGCGUA GGAAUCUGCC CAUUUGAGGG GGAUACCAGU UGGAAACGAC U GUUAAUAC ...String:
UAAACUGAAG AGUUUGAUCC UGGCUCAGAU UGAACGCUGG UGGCAUGCUU AACACAUGCA AGUCGAACGG UAACAGGUCU UAGGAUGCU GACGAGUGGC GGACGGGUGA GUAACGCGUA GGAAUCUGCC CAUUUGAGGG GGAUACCAGU UGGAAACGAC U GUUAAUAC CGCAUAAUAU CUGUGGAUUA AAGGUGGCUU UCGGGCUGUC GCAGAUGGAU GAGCCUGCGU UGGAUUAGCU AG UUGGUGG GGUAAGGGCC CACCAAGGCU ACGAUCCAUA GCUGAUUUGA GAGGAUGAUC AGCCACAUUG GGACUGAGAC ACG GCCCAA ACUCCUACGG GAGGCAGCAG UGGGGAAUAU UGGACAAUGG GGGCAACCCU GAUCCAGCAA UGCCAUGUGU GUGA AGAAG GCCCUAGGGU UGUAAAGCAC UUUAGUUGGG GAGGAAAGCC UCAAGGUUAA UAGCCUUGGG GGAGGACGUU ACCCA AAGA AUAAGCACCG GCUAACUCCG UGCCAGCAGC CGCGGUAAUA CGGGGGGUGC AAGCGUUAAU CGGAAUUACU GGGCGU AAA GGGUCUGUAG GUGGUUUGUU AAGUCAGAUG UGAAAGCCCA GGGCUCAACC UUGGAACUGC AUUUGAUACU GGCAAAC UA GAGUACGGUA GAGGAAUGGG GAAUUUCUGG UGUAGCGGUG AAAUGCGUAG AGAUCAGAAG GAACACCAAU GGCGAAGG C AACAUUCUGG ACCGAUACUG ACACUGAGGG ACGAAAGCGU GGGGAUCAAA CAGGAUUAGA UACCCUGGUA GUCCACGCU GUAAACGAUG AGUACUAGCU GUUGGAGUCG GUGUAAAGGC UCUAGUGGCG CAGCUAACGC GAUAAGUACU CCGCCUGGGG ACUACGGCC GCAAGGCUAA AACUCAAAGG AAUUGACGGG GACCCGCACA AGCGGUGGAG CAUGUGGUUU AAUUCGAUGC A ACGCGAAG AACCUUACCU GGUCUUGACA UCCUGCGAAC UUUCUAGAGA UAGAUUGGUG CCUUCGGGAA CGCAGUGACA GG UGCUGCA CGGCUGUCGU CAGCUCGUGU UGUGAAAUGU UGGGUUAAGU CCCGCAACGA GCGCAACCCC UAUUGAUAGU UAC CAUCAU UAAGUUGGGU ACUCUAUUAA GACUGCCGCU GACAAGGCGG AGGAAGGUGG GGACGACGUC AAGUCAUCAU GGCC CUUAC GACCAGGGCU ACACACGUGC UACAAUGGGU AUUACAGAGG GCUGCGAAGG UGCGAGCUGG AGCGAAACUC AAAAA GGUA CUCUUAGUCC GGAUUGCAGU CUGCAACUCG ACUGCAUGAA GUCGGAAUCG CUAGUAAUCG CAGGUCAGAA UACUGC GGU GAAUACGUUC CCGGGUCUUG UACACACCGC CCGUCACACC AUGGGAGUGG GUUGCUCCAG AAGUAGAUAG CUUAACG AA UGGGCGUUUA CCACGGAGUG AUUCAUGACU GGGGUGAAGU CGUAACAAGG UAGCCGUAGG GGAACCUGCG GCUGGAUC A CCUCCUU

GENBANK: GENBANK: CP063128.1

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Macromolecule #3: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 30.469639 KDa
SequenceString: MIEIKKAKPT SPGRRHVVSV KNTELHTGKP FKGLVEVKKS KAGRNNTGRI TVRHQGGGHK QHYRIVDFKR NKDDITAKVE RIEYDPNRS ANIALVLYAD GERRYIVAPK GLKKDMSVIS GEKVDIAVGN CMPLRNIPLG TVIHNIEMKP KKGAQMIRSA G TFAQLVGK ...String:
MIEIKKAKPT SPGRRHVVSV KNTELHTGKP FKGLVEVKKS KAGRNNTGRI TVRHQGGGHK QHYRIVDFKR NKDDITAKVE RIEYDPNRS ANIALVLYAD GERRYIVAPK GLKKDMSVIS GEKVDIAVGN CMPLRNIPLG TVIHNIEMKP KKGAQMIRSA G TFAQLVGK DNAYAIIRLR SGEMRRVLLD CRAVIGVVSN SEHNLKSLGK AGAKRWRGIR PTVRGVAMNP VDHPHGGGEG RT SGGRHPV TPWGIPTKGY KTRRNKRSNK LIVQKRK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #4: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 22.339348 KDa
SequenceString: MSLGLVGRKC GMTRIFTEDG VSIPVTVVQV EPNKVTQVKT VEKDGYNAIQ VTTGFKKRSN VNKPMAGHYA KASVEPGRGL WEFTVDAAA EYQVGSSFDA TMFEAGQKVD VRGVSKGKGF QGGVKRHNFA TQDATHGNSL SHRVHGSTGQ NQTPGRVFKN K KMAGHLGN ...String:
MSLGLVGRKC GMTRIFTEDG VSIPVTVVQV EPNKVTQVKT VEKDGYNAIQ VTTGFKKRSN VNKPMAGHYA KASVEPGRGL WEFTVDAAA EYQVGSSFDA TMFEAGQKVD VRGVSKGKGF QGGVKRHNFA TQDATHGNSL SHRVHGSTGQ NQTPGRVFKN K KMAGHLGN ENVTIQSLEV VRVDAENGLL LLKGGIPGSV GGDIIVTPAV KS

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #5: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 22.587189 KDa
SequenceString: MDLNIKSLAG QEAGSLGVAE GVFAADYNEA LIHQVVVAYM AGARQGTKAQ KTRSEVSGGG AKPWRQKGTG RARAGTIRSP IFRKGGVTF AAKPKSYKQK VNRKMYSGAV KSILSELLRS GRMTIVEELK LETPKTREFK SVIDSLGVKD VLFVVGVEEF S ENLYLSSR ...String:
MDLNIKSLAG QEAGSLGVAE GVFAADYNEA LIHQVVVAYM AGARQGTKAQ KTRSEVSGGG AKPWRQKGTG RARAGTIRSP IFRKGGVTF AAKPKSYKQK VNRKMYSGAV KSILSELLRS GRMTIVEELK LETPKTREFK SVIDSLGVKD VLFVVGVEEF S ENLYLSSR NLKNVAVCDS VEINPVSLVC FENVVLTKKA IKEIEEKLV

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #6: Large ribosomal subunit protein uL5

MacromoleculeName: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 20.028385 KDa
SequenceString:
MARLKDYYQK ELVAKLKTEL GLDNIMEVPA IKKITLNMGV GDAAKDKKIM TFALNDLTAI AGQKPVVTKS KKSIAGFKIR DGWPIGAKV TLRGDRMYEF LDRLITIAIP RIRDFRGLSA KSFDGRGNYS LGMREQISFP EIDYDKVDSI RGLDISITTT A KNDDQGRA LLKAFGFPFK S

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #7: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 19.106051 KDa
SequenceString:
MSRIGKKPVV IPSGVTINVA AGNKVEVKGA KATLSKTFST DVTFSVADNV ATITPNNNSK NAVAQSGTAR AILSNMVEGV SKGFERKLK IIGVGYRAKA QGNELNLTLG FSHPVVYKLP QGITAETPAP TEIILKGADK ELLGKVASEV REYRKPEPYK G KGVRYEDE YVAKKEAKKK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #8: Large ribosomal subunit protein bL9

MacromoleculeName: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 16.107442 KDa
SequenceString:
MQVILKEKVE NLGVLGDIVN VKPGYARNFL IPFGKAVQAT QANIKAFEAQ KAELEKAEKA RFEAAVAVAD AIKDKVYTIA AQAGEGGKL FGSVGIAEVA EAVSNQSGKK IEKSQVRMPE GVIRSIGEFE LTVHVYTDVD ADIKVNVVAA EA

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #9: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 15.969677 KDa
SequenceString:
MKTFTAKPSN IKREWLLIDA TDKTLGRLAT EVAMILRGKN KPEYTPHMDT GDYVVIVNAE KVAVTGNKRK AKTYYHHTGY IGGIKSVSF EKLIATHPER AIEKAVRGML PRTPLGRTMF KKLKVYAGEA HPHTAQQPKA HNI

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #10: Large ribosomal subunit protein uL14

MacromoleculeName: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.259521 KDa
SequenceString:
MIQMQTELQV ADNSGAKRVE CIKVLGGSHR RYASIGDVIK VTVKEASPRG KAKKGSVYNA VVVRTAKGVR RKDGSKVRFD GNAAVLLNA NGQPIGTRIF GPVTRELRTE KFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #11: Large ribosomal subunit protein uL15

MacromoleculeName: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 15.126674 KDa
SequenceString:
MKLNTLAPAA GSKSAPKRLG RGIGSGLGKT SGKGHKGQKA RSGGYHKVGF EGGQMPLQRR LPKFGFPSAS KRYVAEIRLH ELNNVVADE VTLDTLKDFG LIRKDIKTVK VIASGEIQKA VSLKGIACTK GAKEAIEKAG GKVE

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #12: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 15.742542 KDa
SequenceString:
MLQPKRTKFR KQQKLRNRGL AYRGNKVSFG EFGLQATSRG RITARQIEAG RRAISRHIKR GGKIWIRIFP DKPITQKPLE VRMGKGKGS VEYWVAQIQP GRVLYEITGV KEELAREAFA RAAAKMPVQT TFVEKQVM

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #13: Large ribosomal subunit protein bL17

MacromoleculeName: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 16.81842 KDa
SequenceString:
MRHRKQGRKF GRTSSHRKAM FKNMSASLIN HELIKTTLPK AKELRTIVEP LVTLAKREHK LRQELDTNSN EFKAQSVALR RQAFDFLRN KAAVTKLFEE FGARYAERAG GYTRILKCGY RFGDKAPMAF IELVDRPQVE EAADEE

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #14: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.064138 KDa
SequenceString:
MDKKTARLSR SKRTRIKLRE LGHTRLCVYR TPRHVYAQVI SGDGSTVLVA ASTVEKDVKA KCKYTGNVES AAIVGEIIAD RCKEKGISQ VAFDRSGYKY HGRVKALVEA AREHGLQF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #15: Large ribosomal subunit protein bL19

MacromoleculeName: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.334611 KDa
SequenceString:
MKNKFVELVE KSQLRNDLPE FNPGDSITVN LWIKEGDKQR IQAFKGFVLR KRNRGLHSAF TVRKMSSGMG VERTFQTHSP LIDSIIVEK RADVRRAKLY YMRGLTGKAA RIKEKV

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #16: Large ribosomal subunit protein bL20

MacromoleculeName: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.378632 KDa
SequenceString:
MSRVKRGVTA RARHKKVLNQ AKGYYGARSR VYRVAKQAVI KAGQYAYRDR KVKKRTFRSL WIVRINAAAR QHDISYSQLI NGLNKADVE LDRKALAELA VYNKDAFAAV VEKAKAALA

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #17: Large ribosomal subunit protein bL21

MacromoleculeName: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 11.58568 KDa
SequenceString:
MYAIIKNGGK QYKVKEGEVV KLEKFDLGIG EKVEFDTVLM GQTAAGEVKI GAPTVAGAKV VGEVVEQGRH KKVKIMKFRR RKHSMKQQG HRQYFTAVKV SSISL

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #18: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 12.22333 KDa
SequenceString:
MEVQAKLKFA RISAQKCRLV ADQIRGLPVE QAINLLTFSN KKAAVLIKGV LNSAVANAEH NDGMDVDSLV VSTIFVDEGP TMKRFEARA KGRGNRILKR TSHITVKVAE KK

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #19: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 11.156856 KDa
SequenceString:
MSSQEKLLKT VIRPHVSDKT YGLSDANSTI VFEVARFANK QDVKNAVEKL FEVKVESVNI LNVKGKARRF GRVEGRTKAW KKAYVKLAE GHDINFVGAE

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #20: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 11.498368 KDa
SequenceString:
MNRLKKGDDV IVIAGKDKGR RGVVKSFAKG GSLVLVEGIN IVKKHIKPNP NRGIEGGVVE KELPVDASNV AIFNPATEKA DRVGYKFVD EKKVRYFKSN GELVDL

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #21: Large ribosomal subunit protein bL25

MacromoleculeName: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 10.925688 KDa
SequenceString:
MANFVLKAEK REDLGTGASR RLRRAGKIPA VIYGGEKEAV SVLLDHDKVL HSTEDKEFFS SEITLDIDGK QEKVIIKALQ RHPYKVKLI HADFMRV

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #22: Large ribosomal subunit protein bL27

MacromoleculeName: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 9.055426 KDa
SequenceString:
MAHKKAGGST RNGRDSNPKY LGVKRYGGEL VKAGTIIIRQ RGTKTHPGVN VGCGKDHTLF ALKDGTVKFH TGGALNRKFV SIEE

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #23: Large ribosomal subunit protein bL28

MacromoleculeName: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 8.9605 KDa
SequenceString:
MSKVCIVTGK RPATGNNVSH AQNKTKRRFL PNLHAHRFWV ESENRYIKLR VSSKGMRIID KKGIDTVLSD LRAQGHKI

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #24: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 7.819249 KDa
SequenceString:
MKRKDTLKDY RGKSIDQLQE VKIELLQQLF SLRMQKGTGQ LKKNHLFKSA KRDIARINTI ISEKNK

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #25: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 6.884125 KDa
SequenceString:
MTQAKTFKVT LVKSLIGRKE NHIASARGLG LRKINHTVEV LDTPENRGMA NKIYYMVKIE G

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #26: Large ribosomal subunit protein bL31

MacromoleculeName: Large ribosomal subunit protein bL31 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 8.284602 KDa
SequenceString:
MRQEIHPKYT EVTVTCSCGN TFVTRSTAGK KEMNIDICSE CHPFYTGKQR IVDTAGRVDK FKKRFGGMKK YN

UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #27: Large ribosomal subunit protein bL32

MacromoleculeName: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 6.89578 KDa
SequenceString:
MAVQQVKKSR SKRDMRRSHD SLTNPTLSTD KSTGELHLRH HVSPNGFYKG RKVVDTKSED

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #28: Large ribosomal subunit protein bL33

MacromoleculeName: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 6.158435 KDa
SequenceString:
MREKIRLVSS AKTGHFYTTT KNKKEMPNKM EIKKYDPVVR KHVMYKEAKI K

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #29: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 5.198318 KDa
SequenceString:
MKRTFQPSNL KRKRTHGFRA RMKTLSGRKV IRNRRAKGRA KLAA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #30: Large ribosomal subunit protein bL35

MacromoleculeName: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 7.390867 KDa
SequenceString:
MPKLKTKSGA AKRFKKTGKG GFKHRCANRA HINTKMTTKR KRHLRGMNQV AKVDTTSLVQ QMPYA

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #31: Large ribosomal subunit protein bL36

MacromoleculeName: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 4.437495 KDa
SequenceString:
MKVRASVKKM CRNCKVIKRN RVVRVICTDP RHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #33: Small ribosomal subunit protein uS2

MacromoleculeName: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 24.729816 KDa
SequenceString: SLMKEMLSAG VHFGHKKAFW NPQMKEYIFG INHGVHIINL EKTVPLFQDA VNFVGKTVAN GGKILFVGTK RQAQDIVEAE AKRCGMPFV SHRWLGGMLT NYKTVRQSIK RLAQLEKMRE DGTLESLTKK EMLQNIRTIE KLEKVLGGIK EMGGLPDAIV V IDGNKEHI ...String:
SLMKEMLSAG VHFGHKKAFW NPQMKEYIFG INHGVHIINL EKTVPLFQDA VNFVGKTVAN GGKILFVGTK RQAQDIVEAE AKRCGMPFV SHRWLGGMLT NYKTVRQSIK RLAQLEKMRE DGTLESLTKK EMLQNIRTIE KLEKVLGGIK EMGGLPDAIV V IDGNKEHI AIQEAQKLGI KVVAIVDTNS NPEGIDYIIP GNDDAVKSIS FYMKKFADAV IDAQ

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #34: Small ribosomal subunit protein uS3

MacromoleculeName: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 23.33824 KDa
SequenceString: GQKVNPNGIR LGYIRDWRST WYADSSRYAT KLNEDIKVRE FLHKKLAAAA VSKIQIERPA QNAKITIHTA RSGIVIGKKG EDVEKLRAE VHKLMGIPVQ INIEEVRKPE IDAKLVAESV AQQLEKRVMF RRAMKKAMQA AMKSGAKGIK IMVSGRLGGA E IARSEWAR ...String:
GQKVNPNGIR LGYIRDWRST WYADSSRYAT KLNEDIKVRE FLHKKLAAAA VSKIQIERPA QNAKITIHTA RSGIVIGKKG EDVEKLRAE VHKLMGIPVQ INIEEVRKPE IDAKLVAESV AQQLEKRVMF RRAMKKAMQA AMKSGAKGIK IMVSGRLGGA E IARSEWAR DGRVPLQTFR ADVDYATAEA LTTYGVIGVK VWIYKGEILP G

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #35: Small ribosomal subunit protein uS4

MacromoleculeName: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 23.148707 KDa
SequenceString: ARYLGPKCKL SRREGTDLFL KSGVKANDEK CKMNTAPGQH GARRARLSDY GLQLREKQKV RRMYGILEGQ FKKYYVEASR RKGNTGATL LELLESRLDN VVYRMGFAAT RAEARQLVVH KGIMVNGHTC NVPSAQVKAG DVVAVREKAK KQLRIQNAVE L AKHRKELS ...String:
ARYLGPKCKL SRREGTDLFL KSGVKANDEK CKMNTAPGQH GARRARLSDY GLQLREKQKV RRMYGILEGQ FKKYYVEASR RKGNTGATL LELLESRLDN VVYRMGFAAT RAEARQLVVH KGIMVNGHTC NVPSAQVKAG DVVAVREKAK KQLRIQNAVE L AKHRKELS WIDVNTDSLE GTMKSSPDRS ELSADINEQL IIELYSK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #36: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 17.554285 KDa
SequenceString:
MSNEVKKNEE LIEKLVSVKR HSKTVKGGRI MSFAALTVVG DGKGRIGVGR GKSREVPAAI QKAMENAKKN MVSVNLNNDT LWYPVVSNH GASKVFMQPA SAGTGIIAGG AMRSVFEAVG VHNVLAKTYG STNPANVVRA TIAGLAKIKS PDEIAEKRGL S VEEIQG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #37: Small ribosomal subunit protein bS6

MacromoleculeName: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.075109 KDa
SequenceString:
MKHYEVVLMI HPDQSDQLDA MLGKYRGIIE EKGGKIHRFE DWGRRQLAYP IEKLHKAHYV LFNIECPTES LEKLQESLRY NDAILRRLV IATKEAITEP SVMMESNEKE VI

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #38: Small ribosomal subunit protein uS7

MacromoleculeName: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 17.844732 KDa
SequenceString:
MSRRNRAPKR DILPDPKYKS QVVAKFVNHI MLSGKKSIAE KIVYGAFDKI KAKDASANEV EVFEKALESV SPMVEVKSRR VGGATYQVP VEVRPERRQT LGMRWIIDAA RKRKENTMGD RVAAEILEAV EGRGAAVKKR EDTHKMAEAN KAFAHFRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #39: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 14.431798 KDa
SequenceString:
MSMQDPIADM FTRIRNGLSA EKEFVSVPFS KIKMEIANFL VNEGYIKSCS KGTTSMGHPS IEIELKYHAG APVIEMIKRV SRPSLRIYK SHADLPKVYG GYGVAIVSTS KGLVSDRKAR DLGVGGEIIG YVA

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #40: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 14.764072 KDa
SequenceString:
MSEYNYGTGR RKSSVARVFM KKGTGQFIVN GLPLEQYLCR ETDCMVVKQP LELTNNTDNF DFKVTVKGGG TTGQAGAIRL GVTRALIEY DEELKPALRE AGFVTRDPRK VERKKFGLRK ARRRRQFSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #41: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 11.917983 KDa
SequenceString:
MAINNQRIRI RLKAFDHKLI DISTQEIVDT AKKTGAQVKG PIPLPVRKEK FTILISPHVN KKARDQYEIR THKRLIDIVE PTDKTVDAL MKLDLASGVD VQISLS

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #42: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.786841 KDa
SequenceString:
MAKSVRSSKK KVKRVVPDAV AHIYSSFNNT IVTITDRQGN ALSWATSGGS GFRGSRKSTP FAAQVAAERA ADMALEYGVK NVDVLVKGP GSGRDSAIRA LNAKNLKVTS ITDVTPLPHN GCRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #43: Small ribosomal subunit protein uS12

MacromoleculeName: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.846171 KDa
SequenceString:
MATINQLVNN PRKRSVVKSK VPALKACPQR RGVCTRVYTT TPKKPNSALR KVARVRLTSR FEVTSYIGGE GHNLQEHSVV LIRGGRVKD LPGVRYHIVR GALDTSGVNN RKHGRSKYGT KRPKS

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #44: Small ribosomal subunit protein uS13

MacromoleculeName: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 13.406733 KDa
SequenceString:
MARIAGVNIP VHKHTVIGLT SIYGIGKTRA QQICQTCNVD PTVKIKDLSE EQVESLRTEV AKFTVEGDLR REVSMDIKRL MDLGCFRGR RHRRSLPVRG QRTKTNARTR KGPRKPIKA

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #45: Small ribosomal subunit protein uS14

MacromoleculeName: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 11.74291 KDa
SequenceString:
MAKKSMIQRE LKREKLVAKY AQKRAEFKAI ILDINSTEEQ IWEAQIKLQK LPVNSSASRV QRRCKVTGRP HAVYRKFGLC RNKLREYAM AGDVPGLKKA SW

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #46: Small ribosomal subunit protein uS15

MacromoleculeName: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 10.383016 KDa
SequenceString:
MLTAQDKQKI IKENQLAESD TGSPEVQVAL LTARINDLKG HFEAHKKDNH SRRGLLRLVS QRRKLLDYLH DKDVERYRSL IKKLNIRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #47: Small ribosomal subunit protein bS16

MacromoleculeName: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 9.091747 KDa
SequenceString:
MVVIRMARGG AKKRSFYRIV VADKRSPRDG RFIEKLGFFN PLAKGGEERL KLDVAKAEAW LAKGAQPSDR VASLIKEAKK AA

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #48: Small ribosomal subunit protein uS17

MacromoleculeName: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 9.874555 KDa
SequenceString:
MSDKTRLLEG KVSSVAMDKT VVVRAERYVK HPLYGKFVKK TTKYYVHDEN NECKEGDVIK FKETRPYSKT KKWCLVDIIH REK

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #49: Small ribosomal subunit protein bS18

MacromoleculeName: Small ribosomal subunit protein bS18 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 8.395854 KDa
SequenceString:
MSRRKVCRFT IEGVKEIDYK DVNKLKAYIN ETGKIVPSRV TGTSAKYQRQ LATAIKRARF LALLPYCDRH FN

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #50: Small ribosomal subunit protein uS19

MacromoleculeName: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 10.52345 KDa
SequenceString:
MPRSLKKGPF VDHHLLKKVF EAQESNSKKP IKTWSRRSMI VPDMIGLTIA VHNGQQHVPV LMTEEMVGHK LGEFVVTRNY RGHAADKKA KKK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #51: Small ribosomal subunit protein bS20

MacromoleculeName: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 10.17304 KDa
SequenceString:
MANSKQAKKR IIQAERNRQH NVARRSMMRT FLKKTAYAIE KGDVEAAKEN FTKVVPILDK YASKGLIHKN KAARHKSRLS AKIKALATA A

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #52: Small ribosomal subunit protein bS21B

MacromoleculeName: Small ribosomal subunit protein bS21B / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 7.863384 KDa
SequenceString:
MPSVRIKERE PFDVALRRFK RSCEKAGIVS ELRRREYFEK PTWARKRKKT AAVKRAHKSN IIVKR

UniProtKB: Small ribosomal subunit protein bS21B

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Macromolecule #53: Ribosome hibernation promoting factor

MacromoleculeName: Ribosome hibernation promoting factor / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Francisella tularensis (bacteria)
Molecular weightTheoretical: 11.129688 KDa
SequenceString:
MNIQITGRHV EVTDSIKNYV NEKVGKVGHY FDNITSTKVI LDVEKDHQVA EAIVTVPGSE FVAKAEDKDL YAAIDMLEDK LARQLKKHK DKMRCNHGE

UniProtKB: Ribosome hibernation promoting factor

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Macromolecule #54: (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMI...

MacromoleculeName: (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL
type: ligand / ID: 54 / Number of copies: 9 / Formula: LLL
Molecular weightTheoretical: 449.542 Da

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Macromolecule #55: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 55 / Number of copies: 136 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #56: CHLORAMPHENICOL

MacromoleculeName: CHLORAMPHENICOL / type: ligand / ID: 56 / Number of copies: 1 / Formula: CLM
Molecular weightTheoretical: 323.129 Da
Chemical component information

ChemComp-CLM:
CHLORAMPHENICOL / antibiotic*YM

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Macromolecule #57: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 57 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration8 mg/mL
BufferpH: 7.5 / Details: 20 mM HEPES 7.5
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.03 kPa
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 278 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
SoftwareName: EPU
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.6 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC / Type: NONE
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

9sdr
PDB Unreleased entry

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 270000
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: RANDOM ASSIGNMENT

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Atomic model buiding 1

Initial modelPDB ID:

9sdr
PDB Unreleased entry


Chain - Source name: PDB / Chain - Initial model type: experimental model
SoftwareName: UCSF ChimeraX
RefinementProtocol: AB INITIO MODEL
Output model

PDB-9t6h:
Cryo-EM structure of the 70S ribosome from Francisella tularensis bound to antibiotics chloramphenicol and gentamicin

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