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- EMDB-9306: Human TFIID bound to promoter DNA and TFIIA -

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基本情報

登録情報
データベース: EMDB / ID: EMD-9306
タイトルHuman TFIID bound to promoter DNA and TFIIA
マップデータPrimary map
試料
  • 複合体: General transcription factor IID
    • タンパク質・ペプチド: x 15種
    • DNA: x 2種
キーワードTranscription / DNA / Nuclear / transcription-dna complex
機能・相同性
機能・相同性情報


negative regulation of MHC class I biosynthetic process / spermine transport / SAGA complex assembly / lateral mesodermal cell differentiation / DNA-templated transcription open complex formation / allantois development / pre-snoRNP complex / positive regulation of androgen receptor signaling pathway / TFIIH-class transcription factor complex binding / negative regulation of protein autoubiquitination ...negative regulation of MHC class I biosynthetic process / spermine transport / SAGA complex assembly / lateral mesodermal cell differentiation / DNA-templated transcription open complex formation / allantois development / pre-snoRNP complex / positive regulation of androgen receptor signaling pathway / TFIIH-class transcription factor complex binding / negative regulation of protein autoubiquitination / transcription factor TFTC complex / RNA polymerase I general transcription initiation factor activity / regulation of cell cycle G1/S phase transition / RNA polymerase transcription factor SL1 complex / SLIK (SAGA-like) complex / histone H4K16ac reader activity / negative regulation of MHC class II biosynthetic process / RNA polymerase III general transcription initiation factor activity / RNA polymerase I core promoter sequence-specific DNA binding / hepatocyte differentiation / positive regulation of response to cytokine stimulus / maintenance of protein location in nucleus / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / transcription factor TFIIA complex / C2H2 zinc finger domain binding / RNA Polymerase III Abortive And Retractive Initiation / female germ cell nucleus / male pronucleus / female pronucleus / negative regulation of signal transduction by p53 class mediator / RNA polymerase II general transcription initiation factor binding / nuclear vitamin D receptor binding / RNA polymerase binding / nuclear thyroid hormone receptor binding / regulation of fat cell differentiation / limb development / box C/D snoRNP assembly / SAGA complex / cellular response to ATP / RNA Polymerase I Transcription Termination / transcription preinitiation complex / inner cell mass cell proliferation / response to L-glutamate / RNA polymerase II general transcription initiation factor activity / transcription factor TFIID complex / negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / histone acetyltransferase binding / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / midbrain development / regulation of RNA splicing / ubiquitin conjugating enzyme activity / transcription initiation at RNA polymerase I promoter / histone acetyltransferase activity / aryl hydrocarbon receptor binding / negative regulation of cell cycle / TFIIB-class transcription factor binding / RNA Polymerase I Transcription Initiation / P-TEFb complex binding / MLL1 complex / RNA polymerase II transcribes snRNA genes / negative regulation of ubiquitin-dependent protein catabolic process / : / transcription by RNA polymerase III / positive regulation of transcription initiation by RNA polymerase II / embryonic placenta development / somitogenesis / RNA polymerase II core promoter sequence-specific DNA binding / regulation of DNA repair / core promoter sequence-specific DNA binding / transcription regulator inhibitor activity / RNA polymerase II preinitiation complex assembly / histone acetyltransferase / ovarian follicle development / positive regulation of intrinsic apoptotic signaling pathway / negative regulation of protein kinase activity / estrogen receptor signaling pathway / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / RNA Polymerase II Pre-transcription Events / response to interleukin-1 / TBP-class protein binding / male germ cell nucleus / SIRT1 negatively regulates rRNA expression / regulation of signal transduction by p53 class mediator / nuclear estrogen receptor binding / nuclear receptor binding / transcription initiation at RNA polymerase II promoter / RNA Polymerase I Promoter Escape / DNA-templated transcription initiation / mRNA transcription by RNA polymerase II / promoter-specific chromatin binding / G1/S transition of mitotic cell cycle / euchromatin
類似検索 - 分子機能
TAFII55 protein, conserved region / Transcription initiation factor TFIID subunit 7 / TAFII55 protein conserved region / TAFII55 protein conserved region / Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 8 / Transcription factor TFIID complex subunit 8 C-term / Transcription factor TFIID, subunit 8, C-terminal / Transcription initiation factor TFIID component TAF4 / Transcription initiation factor TFIID component TAF4 family ...TAFII55 protein, conserved region / Transcription initiation factor TFIID subunit 7 / TAFII55 protein conserved region / TAFII55 protein conserved region / Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 8 / Transcription factor TFIID complex subunit 8 C-term / Transcription factor TFIID, subunit 8, C-terminal / Transcription initiation factor TFIID component TAF4 / Transcription initiation factor TFIID component TAF4 family / Transcription initiation factor TFIID component TAF4, C-terminal / TAFH/NHR1 domain superfamily / NHR1 homology to TAF / TAFH/NHR1 domain profile. / TAF homology / TAFH/NHR1 / : / Transcription initiation factor IID, 31kD subunit / Bromodomain associated / Bromodomain transcription factors and PHD domain containing proteins / Bromodomain associated domain / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit A / TFIID subunit TAF5, NTD2 domain superfamily / WD40 associated region in TFIID subunit, NTD2 domain / Transcription initiation factor TAFII31 / TATA box binding protein associated factor (TAF) / Transcription initiation factor TFIID subunit 12 domain / TFIID subunit TAF5, NTD2 domain / Transcription initiation factor TFIID subunit 6 / TAF6, C-terminal HEAT repeat domain superfamily / TAF6 C-terminal HEAT repeat domain / TAF6, C-terminal HEAT repeat domain / Transcription initiation factor TFIID 23-30kDa subunit / Transcription initiation factor TFIID, 23-30kDa subunit / TAFII-230 TBP-binding / Transcription initiation factor TFIID subunit 1, animal / TAFII-230 TBP-binding domain superfamily / TATA box-binding protein binding / Zinc knuckle / Zinc knuckle / Transcription initiation factor TFIID subunit 1, histone acetyltransferase domain / Transcription initiation factor TFIID subunit 1 / Protein of unknown function (DUF3591) / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription factor IIA, alpha/beta subunit / Transcription initiation factor IIA, gamma subunit / Transcription factor IIA, alpha-helical domain / Transcription factor IIA, beta-barrel / Transcription initiation factor IIA, gamma subunit, C-terminal / Transcription initiation factor IIA, gamma subunit, N-terminal / Transcription initiation factor IIA, gamma subunit, helical domain / Transcription initiation factor IIA, gamma subunit / LIS1 homology (LisH) motif profile. / LIS1 homology motif / TATA-box binding protein, eukaryotic / TATA-box binding protein / TATA-box binding protein, conserved site / Transcription factor TFIID (or TATA-binding protein, TBP) / Transcription factor TFIID repeat signature. / Aminopeptidase N-like , N-terminal domain superfamliy / TBP domain superfamily / Peptidase M4/M1, CTD superfamily / TATA box binding protein associated factor / TATA box binding protein associated factor (TAF), histone-like fold domain / Bromodomain, conserved site / Bromodomain signature. / Histone-fold / Bromodomain / bromo domain / Bromodomain (BrD) profile. / Bromodomain / Bromodomain-like superfamily / WD domain, G-beta repeat / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
類似検索 - ドメイン・相同性
Transcription initiation factor TFIID subunit 4 / TATA-box-binding protein / Transcription initiation factor TFIID subunit 1 / Transcription initiation factor TFIID subunit 6 / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / Transcription initiation factor TFIID subunit 10 / Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID subunit 7 / Transcription initiation factor TFIID subunit 12 ...Transcription initiation factor TFIID subunit 4 / TATA-box-binding protein / Transcription initiation factor TFIID subunit 1 / Transcription initiation factor TFIID subunit 6 / Transcription initiation factor IIA subunit 1 / Transcription initiation factor IIA subunit 2 / Transcription initiation factor TFIID subunit 10 / Transcription initiation factor TFIID subunit 5 / Transcription initiation factor TFIID subunit 7 / Transcription initiation factor TFIID subunit 12 / Transcription initiation factor TFIID subunit 9 / Transcription initiation factor TFIID subunit 2 / Transcription initiation factor TFIID subunit 8
類似検索 - 構成要素
生物種Homo sapiens (ヒト)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.5 Å
データ登録者Patel AB / Louder RK
資金援助 米国, 2件
OrganizationGrant number国
Howard Hughes Medical Institute (HHMI) 米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM63072 米国
引用ジャーナル: Science / 年: 2018
タイトル: Structure of human TFIID and mechanism of TBP loading onto promoter DNA.
著者: Avinash B Patel / Robert K Louder / Basil J Greber / Sebastian Grünberg / Jie Luo / Jie Fang / Yutong Liu / Jeff Ranish / Steve Hahn / Eva Nogales /
要旨: The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and ...The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy, chemical cross-linking mass spectrometry, and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA box-binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter.
履歴
登録2018年11月5日-
ヘッダ(付随情報) 公開2018年11月28日-
マップ公開2018年11月28日-
更新2025年5月21日-
現状2025年5月21日処理サイト: RCSB / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 0.03
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 0.03
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-6mzm
  • 表面レベル: 0.03
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_9306.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_9306.map.gz / 形式: CCP4 / 大きさ: 91.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈Primary map
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸Z (Sec.)Y (Row.)X (Col.)
1.32 Å/pix.
x 288 pix.
= 380.16 Å		1.32 Å/pix.
x 288 pix.
= 380.16 Å		1.32 Å/pix.
x 288 pix.
= 380.16 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

ボクセルのサイズX=Y=Z: 1.32 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベル登録者による: 0.03 / ムービー #1: 0.03
最小 - 最大-0.039078873 - 0.118663885
平均 (標準偏差)0.00023230643 (±0.0047335494)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ288288288
Spacing288288288
セルA=B=C: 380.16 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z1.321.321.32
M x/y/z288288288
origin x/y/z0.0000.0000.000
length x/y/z380.160380.160380.160
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS288288288
D min/max/mean-0.0390.1190.000

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添付データ

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ハーフマップ: em-half-volume P1

ファイルemd_9306_half_map_1.map
注釈em-half-volume_P1
投影像・断面図
軸ZYX

投影像

断面 (1/2)
密度ヒストグラム

ヒストグラム

ヒストグラム (対数)

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ハーフマップ: em-half-volume P2

ファイルemd_9306_half_map_2.map
注釈em-half-volume_P2
投影像・断面図
軸ZYX

投影像

断面 (1/2)
密度ヒストグラム

ヒストグラム

ヒストグラム (対数)

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試料の構成要素

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全体 : General transcription factor IID

全体名称: General transcription factor IID
要素
  • 複合体: General transcription factor IID
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 1, TAF1
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 2, TAF2
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 4, TAF4
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 5,TAF5
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 6
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 6
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 7
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 8, TAF8
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 9, TAF9
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 10
    • タンパク質・ペプチド: Transcription initiation factor TFIID subunit 12
    • タンパク質・ペプチド: TATA-box-binding protein
    • DNA: SCP DNA (80-MER)
    • DNA: SCP DNA (80-MER)
    • タンパク質・ペプチド: Transcription initiation factor IIA subunit 1
    • タンパク質・ペプチド: Transcription initiation factor IIA subunit 2
    • タンパク質・ペプチド: Unk

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超分子 #1: General transcription factor IID

超分子名称: General transcription factor IID / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#17
由来(天然)生物種: Homo sapiens (ヒト) / 株: HeLa

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分子 #1: Transcription initiation factor TFIID subunit 1, TAF1

分子名称: Transcription initiation factor TFIID subunit 1, TAF1
タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 57.193742 KDa
配列文字列: IIQHSIPAVE LRQPFFPTHM GPIKLRQFHR PPLKKYSFGA LSQPGPHSVQ PLLKHIKKKA KMREQERQAS GGGEMFFMRT PQDLTGKDG DLILAEYSEE NGPLMMQVGM ATKIKNYYKR KPGKDPGAPD CKYGETVYCH TSPFLGSLHP GQLLQAFENN L FRAPIYLH ...文字列:
IIQHSIPAVE LRQPFFPTHM GPIKLRQFHR PPLKKYSFGA LSQPGPHSVQ PLLKHIKKKA KMREQERQAS GGGEMFFMRT PQDLTGKDG DLILAEYSEE NGPLMMQVGM ATKIKNYYKR KPGKDPGAPD CKYGETVYCH TSPFLGSLHP GQLLQAFENN L FRAPIYLH KMPETDFLII RTRQGYYIRE LVDIFVVGQQ CPLFEVPGPN SKRANTHIRD FLQVFIYRLF WKSKDRPRRI RM EDIKKAF PSHSESSIRK RLKLCADFKR TGMDSNWWVL KSDFRLPTEE EIRAMVSPEQ CCAYYSMIAA EQRLKDAGYG EKS FFAPEE ENEEDFQMKI DDEVRTAPWN TTRAFIAAMK GKCLLEVTGV ADPTGCGEGF SYVKIPNKPT QQKDDKEPQP VKKT VTGTD ADLRRLSLKN AKQLLRKFGV PEEEIKKLSR WEVIDVVRTM STEQARSGEG PMSKFARGSR FSVAEHQERY KEECQ RIFD LQNKVLSSTE VL

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分子 #2: Transcription initiation factor TFIID subunit 2, TAF2

分子名称: Transcription initiation factor TFIID subunit 2, TAF2
タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 137.159984 KDa
配列文字列: MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDP TLEVCHSESK QRNLNYFSNA YAAAVSAVDP DAGNGELCIK VPSELWKHVD ELKVLKIHIN FSLDQPKGGL H FVVPSVEG ...文字列:
MPLTGVEPAR MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDP TLEVCHSESK QRNLNYFSNA YAAAVSAVDP DAGNGELCIK VPSELWKHVD ELKVLKIHIN FSLDQPKGGL H FVVPSVEG SMAERGAHVF SCGYQNSTRF WFPCVDSYSE LCTWKLEFTV DAAMVAVSNG DLVETVYTHD MRKKTFHYML TI PTAASNI SLAIGPFEIL VDPYMHEVTH FCLPQLLPLL KHTTSYLHEV FEFYEEILTC RYPYSCFKTV FIDEAYVEVA AYA SMSIFS TNLLHSAMII DETPLTRRCL AQSLAQQFFG CFISRMSWSD EWVLKGISGY IYGLWMKKTF GVNEYRHWIK EELD KIVAY ELKTGGVLLH PIFGGGKEKD NPASHLHFSI KHPHTLSWEY YSMFQCKAHL VMRLIENRIS MEFMLQVFNK LLSLA STAS SQKFQSHMWS QMLVSTSGFL KSISNVSGKD IQPLIKQWVD QSGVVKFYGS FAFNRKRNVL ELEIKQDYTS PGTQKY VGP LKVTVQELDG SFNHTLQIEE NSLKHDIPCH SKSRRNKKKK IPLMNGEEVD MDLSAMDADS PLLWIRIDPD MSVLRKV EF EQADFMWQYQ LRYERDVVAQ QESILALEKF PTPASRLALT DILEQEQCFY RVRMSACFCL AKIANSMVST WTGPPAMK S LFTRMFCCKS CPNIVKTNNF MSFQSYFLQK TMPVAMALLR DVHNLCPKEV LTFILDLIKY NDNRKNKFSD NYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD PALFKSYAEY GHFVDIRIA ALEAVVDYTK VDRSYEELQW LLNMIQNDPV PYVRHKILNM LTKNPPFTKN MESPLCNEAL VDQLWKLMNS G TSHDWRLR CGAVDLYFTL FGLSRPSCLP LPELGLVLNL KEKKAVLNPT IIPESVAGNQ EAANNPSSHP QLVGFQNPFS SS QDEEEID MDTVHDSQAF ISHHLNMLER PSTPGLSKYR PASSRSALIP QHSAGCDSTP TTKPQWSLEL ARKGTGKEQA PLE MSMHPA ASAPLSVFTK ESTASKHSDH HHHHHHEHKK KKKKHKHKHK HKHKHDSKEK DKEPFTFSSP ASGRSIRSPS LSD

UniProtKB: Transcription initiation factor TFIID subunit 2

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分子 #3: Transcription initiation factor TFIID subunit 4, TAF4

分子名称: Transcription initiation factor TFIID subunit 4, TAF4
タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 104.052086 KDa
配列文字列: HVVSGSPAGA AGAGPAAPAE GAPGAAPEPP PAGRARPGGG GPQRPGPPSP RRPLVPAGPA PPAAKLRPPP EGSAGSCAPV PAAAAVAAG PEPAPAGPAK PAGPAALAAR AGPGPGPGPG PGPGPGPGKP AGPGAAQTLN GSAALLNSHH AAAPAVSLVN N GPAALLPL ...文字列:
HVVSGSPAGA AGAGPAAPAE GAPGAAPEPP PAGRARPGGG GPQRPGPPSP RRPLVPAGPA PPAAKLRPPP EGSAGSCAPV PAAAAVAAG PEPAPAGPAK PAGPAALAAR AGPGPGPGPG PGPGPGPGKP AGPGAAQTLN GSAALLNSHH AAAPAVSLVN N GPAALLPL PKPAAPGTVI QTPPFVGAAA PPAPAAPSPP AAPAPAAPAA APPPPPPAPA TLARPPGHPA GPPTAAPAVP PP AAAQNGG SAGAAPAPAP AAGGPAGVSG QPGPGAAAAA PAPGVKAESP KRVVQAAPPA AQTLAASGPA STAASMVIGP TMQ GALPSP AAVPPPAPGT PTGLPKGAAG AVTQSLSRTP TATTSGIRAT LTPTVLAPRL PQPPQNPTNI QNFQLPPGMV LVRS ENGQL LMIPQQALAQ MQAQAHAQPQ TTMAPRPATP TSAPPVQIST VQAPGTPIIA RQVTPTTIIK QVSQAQTTVQ PSATL QRSP GVQPQLVLGG AAQTASLGTA TAVQTGTPQR TVPGATTTSS AATETMENVK KCKNFLSTLI KLASSGKQST ETAANV KEL VQNLLDGKIE AEDFTSRLYR ELNSSPQPYL VPFLKRSLPA LRQLTPDSAA FIQQSQQQPP PPTSQATTAL TAVVLSS SV QRTAGKTAAT VTSALQPPVL SLTQPTQVGV GKQGQPTPLV IQQPPKPGAL IRPPQVTLTQ TPMVALRQPH NRIMLTTP Q QIQLNPLQPV PVVKPAVLPG TKALSAVSAQ AAAAQKNKLK EPGGGSFRDD DDINDVASMA GVNLSEESAR ILATNSELV GTLTRSCKDE TFLLQAPLQR RILEIGKKHG ITELHPDVVS YVSHATQQRL QNLVEKISET AQQKNFSYKD DDRYEQASDV RAQLKFFEQ LDQIEKQRKD EQEREILMRA AKSRSRQEDP EQLRLKQKAK EMQQQELAQM RQRDANLTAL AAIGPRKKRK V DCPGPGSG AEGSGPGSVV PGSSGVGTPR QFTRQRITRV NLRDLIFCLE NERETSHSLL LYKAFLK

UniProtKB: Transcription initiation factor TFIID subunit 4

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分子 #4: Transcription initiation factor TFIID subunit 5,TAF5

分子名称: Transcription initiation factor TFIID subunit 5,TAF5
タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 85.785164 KDa
配列文字列: MAALAEEQTE VAVKLEPEGP PTLLPPQAGD GAGEGSGGTT NNGPNGGGGN VAASSSTGGD GGTPKPTVAV SAAAPAGAAP VPAAAPDAG APHDRQTLLA VLQFLRQSKL REAEEALRRE AGLLEEAVAG SGAPGEVDSA GAEVTSALLS RVTASAPGPA A PDPPGTGA ...文字列:
MAALAEEQTE VAVKLEPEGP PTLLPPQAGD GAGEGSGGTT NNGPNGGGGN VAASSSTGGD GGTPKPTVAV SAAAPAGAAP VPAAAPDAG APHDRQTLLA VLQFLRQSKL REAEEALRRE AGLLEEAVAG SGAPGEVDSA GAEVTSALLS RVTASAPGPA A PDPPGTGA SGATVVSGSA SGPAAPGKVG SVAVEDQPDV SAVLSAYNQQ GDPTMYEEYY SGLKHFIECS LDCHRAELSQ LF YPLFVHM YLELVYNQHE NEAKSFFEKF HGDQECYYQD DLRVLSSLTK KEHMKGNETM LDFRTSKFVL RISRDSYQLL KRH LQEKQN NQIWNIVQEH LYIDIFDGMP RSKQQIDAMV GSLAGEAKRE ANKSKVFFGL LKEPEIEVPL DDEDEEGENE EGKP KKKKP KKDSIGSKSK KQDPNAPPQN RIPLPELKDS DKLDKIMNMK ETTKRVRLGP DCLPSICFYT FLNAYQGLTA VDVTD DSSL IAGGFADSTV RV(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)KILYGH SGPVYGASFS PDRN YLLSS SEDGTVRLWS LQTFTCLVGY KGHNYPVWDT QFSPYGYYFV SGGHDRVARL WATDHYQPLR IFAGHLADVN CTRFH PNSN YVATGSADRT VRLWDVLNGN CVRIFTGHKG PIHSLTFSPN GRFLATGATD GRVLLWDIGH GLMVGELKGH TDTVCS LRF SRDGEILASG SMDNTVRLWD AIKAFEDLET DDFTTATGHI NLPENSQELL LGTYMTKSTP VVHLHFTRRN LVLAAGA YS PQ

UniProtKB: Transcription initiation factor TFIID subunit 5, Transcription initiation factor TFIID subunit 5

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分子 #5: Transcription initiation factor TFIID subunit 6

分子名称: Transcription initiation factor TFIID subunit 6 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 72.749297 KDa
配列文字列: MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA ...文字列:
MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA KPGQEEDGPL KGKGQGATTA DGKGKEKKAP PLLEGAPLRL KPRSIHELSV EQQLYYKEIT EACVGSCEAK RA EALQSIA TDPGLYQMLP RFSTFISEGV RVNVVQNNLA LLIYLMRMVK ALMDNPTLYL EKYVHELIPA VMTCIVSRQL CLR PDVDNH WALRDFAARL VAQICKHFST TTNNIQSRIT KTFTKSWVDE KTPWTTRYGS IAGLAELGHD VIKTLILPRL QQEG ERIRS VLDGPVLSNI DRIGADHVQS LLLKHCAPVL AKLRPPPDNQ DAYRAEFGSL GPLLCSQVVK ARAQAALQAQ QVNRT TLTI TQPRPTLTLS QAPQPGPRTP GLLKVPGSIA LPVQTLVSAR AAAPPQPSPP PTKFIVMSSS SSAPSTQQVL SLSTSA PGS GSTTTSPVTT TVPSVQPIVK LVSTATTAPP STAPSGPGSV QKYIVVSLPP TGEGKGGPTS HPSPVPPPAS SPSPLSG SA LCGGKQEAGD SPPPAPGTPK ANGSQPNSGS PQPAP

UniProtKB: Transcription initiation factor TFIID subunit 6

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分子 #6: Transcription initiation factor TFIID subunit 6

分子名称: Transcription initiation factor TFIID subunit 6 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 72.365836 KDa
配列文字列: MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA ...文字列:
MAEEKKLKLS NTVLPSESMK VVAESMGIAQ IQEETCQLLT DEVSYRIKEI AQDALKFMHM GKRQKLTTSD IDYALKLKNV EPLYGFHAQ EFIPFRFASG GGRELYFYEE KEVDLSDIIN TPLPRVPLDV CLKAHWLSIE GCQPAIPENP PPAPKEQQKA E ATEPLKSA KPGQEEDGPL KGKGQGATTA DGKGKEKKAP PLLEGAPL(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)S VEQQLYYKEI TEACVGSCEA KRAEALQSIA TDPGLYQMLP RFSTFISEGV RVNVVQNNL ALLIYLMRMV KALMDNPTLY LEKYVHELIP AVMTCIVSRQ LCLRPDVDNH WALRDFAARL VAQICKHFST TTNNIQSRIT KTFTKSWVD EKTPWTTRYG SIAGLAELGH DVIKTLILPR LQQEGERIRS VLDGPVLSNI DRIGADHVQS LLLKHCAPVL A KLRPPPDN QDAYRAEFGS LGPLLCSQVV KARAQAALQA QQVNRTTLTI TQPRPTLTLS QAPQPGPRTP GLLKVPGSIA LP VQTLVSA RAAAPPQPSP PPTKFIVMSS SSSAPSTQQV LSLSTSAPGS GSTTTSPVTT TVPSVQPIVK LVSTATTAPP STA PSGPGS VQKYIVVSLP PTGEGKGGPT SHPSPVPPPA SSPSPLSGSA LCGGKQEAGD SPPPAPGTPK ANGSQPNSGS PQPA P

UniProtKB: Transcription initiation factor TFIID subunit 6, Transcription initiation factor TFIID subunit 6

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分子 #7: Transcription initiation factor TFIID subunit 7

分子名称: Transcription initiation factor TFIID subunit 7 / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 40.325117 KDa
配列文字列: MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTA DICQMLVSTV DGDLYPPVEE PVASTDPKAS KKKDKDKEKK FIWNHGITLP LKNVRKRRFR KTAKKKYIES P DVEKEVKR ...文字列:
MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTA DICQMLVSTV DGDLYPPVEE PVASTDPKAS KKKDKDKEKK FIWNHGITLP LKNVRKRRFR KTAKKKYIES P DVEKEVKR LLSTDAEAVS TRWEIIAEDE TKEAENQGLD ISSPGMSGHR QGHDSLEHDE LREIFNDLSS SSEDEDETQH QD EEDINII DTEEDLERQL QDKLNESDEQ HQENEGTNQL VMGIQKQIDN MKGKLQETQD RAKRQEDLIM KVENLALKNR FQA VLDELK QKEDREKEQL SSLQEELESL LEK

UniProtKB: Transcription initiation factor TFIID subunit 7

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分子 #8: Transcription initiation factor TFIID subunit 8, TAF8

分子名称: Transcription initiation factor TFIID subunit 8, TAF8
タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 32.975344 KDa
配列文字列: MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPT LSDIVVTLVE MGFNVDTLPA YAKRSQRMVI T(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) ...文字列:
MADAAATAGA GGSGTRSGSK QSTNPADNYH LARRRTLQVV VSSLLTEAGF ESAEKASVET LTEMLQSYIS EIGRSAKSYC EHTARTQPT LSDIVVTLVE MGFNVDTLPA YAKRSQRMVI T(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)YQVLR EKAASQRRDV ERALTRFMAK TGETQSLFK DDVSTFPLIA ARPFTIPYLT ALLPSELEMQ QMEETDSSEQ DEQTDTENLA LHISMEDSGA EKENTSVLQQ N PSLSGSRN GEENIIDNPY LRPVKKPKIR RKKSLS

UniProtKB: Transcription initiation factor TFIID subunit 8

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分子 #9: Transcription initiation factor TFIID subunit 9, TAF9

分子名称: Transcription initiation factor TFIID subunit 9, TAF9
タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 28.830689 KDa
配列文字列: MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPR DFLLDIARQR NQTPLPLIKP (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PDR YCLTAPNYRL KSL QKKAST ...文字列:
MESGKTASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPR DFLLDIARQR NQTPLPLIKP (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PDR YCLTAPNYRL KSL QKKAST SAGRITVPRL SVGSVTSRPS TPTLGTPTPQ TMSVSTKVGT PMSLTGQRFT VQMPTSQSPA VKASIPATSA VQNV LINPS LIGSKNILIT TNMMSSQNTA NESSNALKRK REDDDDDDDD DDDYDNL

UniProtKB: Transcription initiation factor TFIID subunit 9

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分子 #10: Transcription initiation factor TFIID subunit 10

分子名称: Transcription initiation factor TFIID subunit 10 / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 21.731248 KDa
配列文字列: MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPE GAISNGVYVL PSAANGDVKP VVSSTPLVDF LMQLEDYTPT IPDAVTGYYL NRAGFEASDP RIIRLISLAA Q KFISDIAN ...文字列:
MSCSGSGADP EAAPASAASA PGPAPPVSAP AALPSSTAAE NKASPAGTAG GPGAGAAAGG TGPLAARAGE PAERRGAAPV SAGGAAPPE GAISNGVYVL PSAANGDVKP VVSSTPLVDF LMQLEDYTPT IPDAVTGYYL NRAGFEASDP RIIRLISLAA Q KFISDIAN DALQHCKMKG TASGSSRSKS KDRKYTLTME DLTPALSEYG INVKKPHYFT

UniProtKB: Transcription initiation factor TFIID subunit 10

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分子 #11: Transcription initiation factor TFIID subunit 12

分子名称: Transcription initiation factor TFIID subunit 12 / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 17.948467 KDa
配列文字列:
MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL SPENNQVLTK KKLQDLVREV DPNEQLDEDV EEMLLQIAD DFIESVVTAA CQLARHRKSS TLEVKDVQLH LERQWNMWIP GFGSEEIRPY KKACTTEAHK QRMALIRKTT K K

UniProtKB: Transcription initiation factor TFIID subunit 12

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分子 #12: TATA-box-binding protein

分子名称: TATA-box-binding protein / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 37.729938 KDa
配列文字列: MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQ QQQQQQAVAA AAVQQSTSQQ ATQGTSGQAP QLFHSQTLTT APLPGTTPLY PSPMTPMTPI TPATPASESS G IVPQLQNI ...文字列:
MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQ QQQQQQAVAA AAVQQSTSQQ ATQGTSGQAP QLFHSQTLTT APLPGTTPLY PSPMTPMTPI TPATPASESS G IVPQLQNI VSTVNLGCKL DLKTIALRAR NAEYNPKRFA AVIMRIREPR TTALIFSSGK MVCTGAKSEE QSRLAARKYA RV VQKLGFP AKFLDFKIQN MVGSCDVKFP IRLEGLVLTH QQFSSYEPEL FPGLIYRMIK PRIVLLIFVS GKVVLTGAKV RAE IYEAFE NIYPILKGFR KTT

UniProtKB: TATA-box-binding protein

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分子 #15: Transcription initiation factor IIA subunit 1

分子名称: Transcription initiation factor IIA subunit 1 / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 10.675194 KDa
組換発現生物種: Escherichia coli (大腸菌)
配列文字列:
TVPKLYRSVI EDVINDVRDI FLDDGVDEQV LMELKTLWEN KLMDTENVVV CQYDKIHRSK NKWKFHLKDG IMNLNGRDYI FSKAIGDAE W

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分子 #16: Transcription initiation factor IIA subunit 2

分子名称: Transcription initiation factor IIA subunit 2 / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 11.275824 KDa
組換発現生物種: Escherichia coli (大腸菌)
配列文字列:
YQLYRNTTLG NSLQESLDEL IQSQQITPQL ALQVLLQFDK AINAALAQRV RNRVNFRGSL NTYRFCDNVW TFVLNDVEFR EVTELIKVD KVKIVACD

UniProtKB: Transcription initiation factor IIA subunit 2

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分子 #17: Unk

分子名称: Unk / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 20.272906 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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分子 #13: SCP DNA (80-MER)

分子名称: SCP DNA (80-MER) / タイプ: dna / ID: 13 / コピー数: 1 / 分類: DNA
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 24.498586 KDa
配列文字列: (DC)(DC)(DG)(DT)(DA)(DG)(DG)(DC)(DA)(DC) (DG)(DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG) (DC)(DT)(DC)(DG)(DA)(DG)(DT)(DG)(DT) (DT)(DC)(DG)(DA)(DT)(DC)(DG)(DC)(DG)(DA) (DC) (DT)(DG)(DA)(DG)(DG) ...文字列:
(DC)(DC)(DG)(DT)(DA)(DG)(DG)(DC)(DA)(DC) (DG)(DT)(DC)(DT)(DG)(DC)(DT)(DC)(DG)(DG) (DC)(DT)(DC)(DG)(DA)(DG)(DT)(DG)(DT) (DT)(DC)(DG)(DA)(DT)(DC)(DG)(DC)(DG)(DA) (DC) (DT)(DG)(DA)(DG)(DG)(DA)(DC)(DG) (DA)(DA)(DC)(DG)(DC)(DG)(DC)(DC)(DC)(DC) (DC)(DA) (DC)(DC)(DC)(DC)(DC)(DT)(DT) (DT)(DT)(DA)(DT)(DA)(DG)(DG)(DC)(DG)(DC) (DC)(DC)(DT)

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分子 #14: SCP DNA (80-MER)

分子名称: SCP DNA (80-MER) / タイプ: dna / ID: 14 / コピー数: 1 / 分類: DNA
由来(天然)生物種: Homo sapiens (ヒト)
分子量理論値: 24.854842 KDa
配列文字列: (DA)(DG)(DG)(DG)(DC)(DG)(DC)(DC)(DT)(DA) (DT)(DA)(DA)(DA)(DA)(DG)(DG)(DG)(DG)(DG) (DT)(DG)(DG)(DG)(DG)(DG)(DC)(DG)(DC) (DG)(DT)(DT)(DC)(DG)(DT)(DC)(DC)(DT)(DC) (DA) (DG)(DT)(DC)(DG)(DC) ...文字列:
(DA)(DG)(DG)(DG)(DC)(DG)(DC)(DC)(DT)(DA) (DT)(DA)(DA)(DA)(DA)(DG)(DG)(DG)(DG)(DG) (DT)(DG)(DG)(DG)(DG)(DG)(DC)(DG)(DC) (DG)(DT)(DT)(DC)(DG)(DT)(DC)(DC)(DT)(DC) (DA) (DG)(DT)(DC)(DG)(DC)(DG)(DA)(DT) (DC)(DG)(DA)(DA)(DC)(DA)(DC)(DT)(DC)(DG) (DA)(DG) (DC)(DC)(DG)(DA)(DG)(DC)(DA) (DG)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DA) (DC)(DG)(DG)

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

濃度.2 mg/mL
緩衝液pH: 7.9
構成要素:
濃度式
10.0 mMHEPES
10.0 mMMgCl
1.0 mMDTT
3.0 %Trehalose
50.0 mMKCl
0.0125 %NP-40
凍結凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277.15 K / 装置: FEI VITROBOT MARK IV / 詳細: BT 4s; BF 15N.

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電子顕微鏡法

顕微鏡FEI TITAN
撮影フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k)
平均電子線量: 40.0 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系C2レンズ絞り径: 50.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm
試料ステージ試料ホルダーモデル: GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
ホルダー冷却材: NITROGEN

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画像解析

CTF補正ソフトウェア - 名称: Gctf / タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION
初期モデルモデルのタイプ: OTHER
最終 再構成想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 7.5 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 使用した粒子像数: 25180
初期 角度割当タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION
最終 角度割当タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る