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- EMDB-8937: Cryo-EM Structure of Mycobacterium smegmatis C(minus) 50S ribosom... -

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Basic information

Entry
Database: EMDB / ID: EMD-8937
TitleCryo-EM Structure of Mycobacterium smegmatis C(minus) 50S ribosomal subunit
Map dataCryo-EM structure of Mycobacterium smegmatis C(minus) 50S ribosomal subunit
Sample
  • Complex: Mycobacterium smegmatis C(minus) 70S ribosome with MPY
    • RNA: x 2 types
    • Protein or peptide: x 32 types
KeywordsHibernation factor complex / RIBOSOME
Function / homology
Function and homology information


large ribosomal subunit rRNA binding / large ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / cytoplasm
Similarity search - Function
Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L10 / : / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 signature. ...Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein L10 / : / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L10-like domain superfamily / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L11, N-terminal / Ribosomal protein L17 signature. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11, RNA binding domain / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / L28p-like / Ribosomal protein L20 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal proteins 50S L24/mitochondrial 39S L24 / Ribosomal protein L17 / Ribosomal protein L17 superfamily / Ribosomal protein L17
Similarity search - Domain/homology
Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 ...Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL36 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL13 / Uncharacterized protein / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33B / 50S ribosomal protein L28 / 50S ribosomal protein L31 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein uL18
Similarity search - Component
Biological speciesMycobacterium smegmatis str. MC2 155 (bacteria) / Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.42 Å
AuthorsSharma MR / Li Y / Korripella R / Yang Y / Kaushal PS / Lin Q / Wade JT / Gray AG / Derbyshire KM / Agrawal RK / Ojha A
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)GM061576 United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)AI107595 United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)AI097191 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2018
Title: Zinc depletion induces ribosome hibernation in mycobacteria.
Authors: Yunlong Li / Manjuli R Sharma / Ravi K Koripella / Yong Yang / Prem S Kaushal / Qishan Lin / Joseph T Wade / Todd A Gray / Keith M Derbyshire / Rajendra K Agrawal / Anil K Ojha /
Abstract: Bacteria respond to zinc starvation by replacing ribosomal proteins that have the zinc-binding CXXC motif (C+) with their zinc-free (C-) paralogues. Consequences of this process beyond zinc ...Bacteria respond to zinc starvation by replacing ribosomal proteins that have the zinc-binding CXXC motif (C+) with their zinc-free (C-) paralogues. Consequences of this process beyond zinc homeostasis are unknown. Here, we show that the C- ribosome in is the exclusive target of a bacterial protein Y homolog, referred to as mycobacterial-specific protein Y (MPY), which binds to the decoding region of the 30S subunit, thereby inactivating the ribosome. MPY binding is dependent on another mycobacterial protein, MPY recruitment factor (MRF), which is induced on zinc depletion, and interacts with C- ribosomes. MPY binding confers structural stability to C- ribosomes, promoting survival of growth-arrested cells under zinc-limiting conditions. Binding of MPY also has direct influence on the dynamics of aminoglycoside-binding pockets of the C- ribosome to inhibit binding of these antibiotics. Together, our data suggest that zinc limitation leads to ribosome hibernation and aminoglycoside resistance in mycobacteria. Furthermore, our observation of the expression of the proteins of C- ribosomes in in a mouse model of infection suggests that ribosome hibernation could be relevant in our understanding of persistence and drug tolerance of the pathogen encountered during chemotherapy of TB.
History
DepositionJul 5, 2018-
Header (metadata) releaseOct 3, 2018-
Map releaseOct 3, 2018-
UpdateMar 13, 2024-
Current statusMar 13, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.033
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.033
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6dzp
  • Surface level: 0.033
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8937.png

Downloads & links

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Map

FileDownload / File: emd_8937.map.gz / Format: CCP4 / Size: 357 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM structure of Mycobacterium smegmatis C(minus) 50S ribosomal subunit
Voxel sizeX=Y=Z: 1.07 Å
Density
Contour LevelBy AUTHOR: 0.033 / Movie #1: 0.033
Minimum - Maximum-0.19380207 - 0.29954392
Average (Standard dev.)0.00042198613 (±0.0072495956)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions454454454
Spacing454454454
CellA=B=C: 485.78003 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.071.071.07
M x/y/z454454454
origin x/y/z0.0000.0000.000
length x/y/z485.780485.780485.780
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS454454454
D min/max/mean-0.1940.3000.000

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Supplemental data

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Sample components

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Entire : Mycobacterium smegmatis C(minus) 70S ribosome with MPY

EntireName: Mycobacterium smegmatis C(minus) 70S ribosome with MPY
Components
  • Complex: Mycobacterium smegmatis C(minus) 70S ribosome with MPY
    • RNA: 23S rRNA23S ribosomal RNA
    • RNA: 5S rRNA5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L10
    • Protein or peptide: 50S ribosomal protein L11
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33 2Ribosome
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: Uncharacterized protein

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Supramolecule #1: Mycobacterium smegmatis C(minus) 70S ribosome with MPY

SupramoleculeName: Mycobacterium smegmatis C(minus) 70S ribosome with MPY
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Details: Cryo-EM structure of Mycobacterium smegmatis C (minus) 50S ribosomal subunit
Source (natural)Organism: Mycobacterium smegmatis str. MC2 155 (bacteria)

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Macromolecule #1: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Mycobacterium smegmatis str. MC2 155 (bacteria) / Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 1.01183475 MDa
SequenceString: AAGUGUUUAA GGGCGCAUGG UGGAUGCCUU GGCACUGGGA GCCGAUGAAG GACGUAGGAG GCUGCGAUAA GCCUCGGGGA GCUGUCAAC CGAGCGUUGA UCCGAGGAUG UCCGAAUGGG GAAACCCGGC ACGAGUGAUG UCGUGUCACC AGGCGCUGAA U AUAUAGGC ...String:
AAGUGUUUAA GGGCGCAUGG UGGAUGCCUU GGCACUGGGA GCCGAUGAAG GACGUAGGAG GCUGCGAUAA GCCUCGGGGA GCUGUCAAC CGAGCGUUGA UCCGAGGAUG UCCGAAUGGG GAAACCCGGC ACGAGUGAUG UCGUGUCACC AGGCGCUGAA U AUAUAGGC GUCUGGGGGG AACGCGGGGA AGUGAAACAU CUCAGUACCC GUAGGAAGAG AAAACAAAAU GUGAUUCCGU GA GUAGUGG CGAGCGAAAG CGGAGGAUGG CUAAACCGUA UGCAUGUGAU ACCGGGUAGG GGUUGUGUGU GCGGGGUUGU GGG ACCUAU CUUUCCGGCU CUACCUGGCU GGAGGGCAGU GAGAAAAUGU UGUGGUUAGC GGAAAUGGCU UGGGAUGGCC UGCC GUAGA CGGUGAGAGC CCGGUACGUG AAAACCCGAC GUCUGUCUUG AUGGUGUUCC CGAGUAGCAG CGGGCCCGUG GAAUC UGCU GUGAAUCUGC CGGGACCACC CGGUAAGCCU GAAUACUUCC CAGUGACCGA UAGCGGAUUA GUACCGUGAG GGAAUG GUG AAAAGUACCC CGGGAGGGGA GUGAAAGAGU ACCUGAAACC GUGCGCUUAC AAUCCGUCAG AGCCCUCGAC GUGUCGU GG GGUGAUGGCG UGCCUUUUGA AGAAUGAGCC UGCGAGUCAG GGACAUGUCG CGAGGUUAAC CCGGGUGGGG UAGCCGCA G CGAAAGCGAG UCUGAAUAGG GCGUAUCCAC ACAAGAGUGU GUGGUGUAGU GGUGUGUUCU GGACCCGAAG CGGAGUGAU CUACCCAUGG CCAGGGUGAA GCGCGGGUAA GACCGCGUGG AGGCCCGAAC CCACUUAGGU UGAAGACUGA GGGGAUGAGC UGUGGGUAG GGGUGAAAGG CCAAUCAAAC UCCGUGAUAG CUGGUUCUCC CCGAAAUGCA UUUAGGUGCA GCGUCGCAUG U UUCUUGCC GGAGGUAGAG CUACUGGAUG GCCGAUGGGC CCCACAGGGU UACUGACGUC AGCCAAACUC CGAAUGCCGG UA AGUCCAA GAGUGCGGCA GUGAGACGGC GGGGGAUAAG CUCCGUGCGU CGAGAGGGAA ACAGCCCAGA UCGCCGGCUA AGG CCCCUA AGCGUGUGCU AAGUGGAAAA GGAUGUGCAG UCGCGAAGAC AACCAGGAGG UUGGCUUAGA AGCAGCCACC CUUG AAAGA GUGCGUAAUA GCUCACUGGU CAAGUGAUUG UGCGCCGAUA AUGUAGCGGG GCUCAAGCAC ACCGCCGAAG CCGCG GCAG CCAACGUGUU GGCUGGGUAG GGGAGCGUCC UGCAUCCGGU GAAGCCGCCG AGUGAUCGAG UGGUGGAGGG UGUGGG AGU GAGAAUGCAG GCAUGAGUAG CGAUUAGGCA AGUGAGAACC UUGCCCGCCG AAAGACCAAG GGUUCCUGGG CCAGGCC AG UCCGCCCAGG GUGAGUCGGG ACCUAAGGCG AGGCCGACAG GCGUAGUCGA UGGACAACGG GUUGAUAUUC CCGUACCC G UGUAUGUGCG UCCAUGAUGA AUCAGCGGUA CUAACCAUCC AAAACCACCG UGACCGCACC UUUCGGGGUG UGGCGUUGG UGGGGCUGCA UGGGACCUUC GUUGGUAGUA GUCAAGCGAU GGGGUGACGC AGGAAGGUAG CCGUACCGGU CAGUGGUAAU ACCGGGGUA AGCCUGUAGG GAGUCAGAUA GGUAAAUCCG UCUGGCAUAU AUCCUGAGAG GUGAUGCAUA GCCGAGUGAG G CGAAUUCG GUGAUCCUAU GCUGCCGAGA AAAGCCUCUA GCGAGGACAU ACACGGCCCG UACCCCAAAC CAACACAGGU GG UCAGGUA GAGAAUACUA AGGCGUACGA GUGAACUAUG GUUAAGGAAC UCGGCAAAAU GCCCCCGUAA CUUCGGGAGA AGG GGGACC CACAUGGCGU GUAAGCCUUU ACGGCCCAAG CGUGAGUGGG UGGCACAAAC CAGUGAGAAG CGACUGUUUA CUAA AAACA CAGGUCCGUG CGAAGUCGCA AGACGAUGUA UACGGACUGA CGCCUGCCCG GUGCUGGAAG GUUAAGAGGA CCCGU UAAC UCCCUUUGGG GGUGAAGCGG AGAAUUUAAG CCCCAGUAAA CGGCGGUGGU AACUAUAACC AUCCUAAGGU AGCGAA AUU CCUUGUCGGG UAAGUUCCGA CCUGCACGAA UGGCGUAACG ACUUCUCAAC UGUCUCAACC AUAGACUCGG CGAAAUU GC ACUACGAGUA AAGAUGCUCG UUACGCGCGG CAGGACGAAA AGACCCCGGG ACCUUCACUA CAACUUGGUA UUGGUGCU C GAUACGGUUU GUGUAGGAUA GGUGGGAGAC UGUGAAGCUC ACACGCCAGU GUGGGUGGAG UCGUUGUUGA AAUACCACU CUGAUCGUAU UGGGCCUCUA ACCUCGGACC GUAUAUCCGG UUCAGGGACA GUGCCUGGUG GGUAGUUUAA CUGGGGCGGU UGCCUCCUA AAAUGUAACG GAGGCGCCCA AAGGUUCCCU CAACCUGGAC GGCAAUCAGG UGUUGAGUGU AAGUGCACAA G GGAGCUUG ACUGCGAGAC GGACAUGUCG AGCAGGGACG AAAGUCGGGA CUAGUGAUCC GGCACCUCUG AGUGGAAGGG GU GUCGCUC AACGGAUAAA AGGUACCCCG GGGAUAACAG GCUGAUCUUC CCCAAGAGUC CAUAUCGACG GGAUGGUUUG GCA CCUCGA UGUCGGCUCG UCGCAUCCUG GGGCUGGAGC AGGUCCCAAG GGUUGGGCUG UUCGCCCAUU AAAGCGGCAC GCGA GCUGG GUUUAGAACG UCGUGAGACA GUUCGGUCUC UAUCCGCCGC GCGCGUCAGA AGCUUGAGGA AACCUGUCCC UAGUA CGAG AGGACCGGGA CGGACGAACC UCUGGUAUAC CAGUUGUCCC ACCAGGGGCA CGGCUGGAUA GCCACGUUCG GACAGG AUA ACCGCUGAAA GCAUCUAAGC GGGAAACCUC UUCCAAGACC AGGCUUCUCA CCCUCUAGGA GGGAUAAGGC CCCCCGC AG ACCACGGGAU UGAUAGACCA GACCUGGAAG CCUAGUAAUA GGUGCAGGGA ACUGGCACUA ACCGGCCGAA AACUUAC

GENBANK: GENBANK: CP000480.1

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Macromolecule #2: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Mycobacterium smegmatis str. MC2 155 (bacteria) / Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 38.061816 KDa
SequenceString:
GUUACGGCGG UCCAUAGCGG CAGGGAAACG CCCGGUCCCA UCCCGAACCC GGAAGCUAAG CCUGCCAGCG CCGAUGAUAC UACCCAUCC GGGUGGAAAA GUAGGACACC GCCGAACAC

GENBANK: GENBANK: CP000480.1

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 30.425945 KDa
SequenceString: MGIRKYKPTT PGRRGASVSD FAEITRSTPE KSLVRPLHGK GGRNAHGRIT TRHKGGGHKR AYRVIDFRRH DKDGVNAKVA HIEYDPNRT ANIALLHYLD GEKRYIIAPQ GLKQGDVIES GANADIKPGN NLPLRNIPAG TVIHAVELRP GGGAKLARSA G VSIQLLGK ...String:
MGIRKYKPTT PGRRGASVSD FAEITRSTPE KSLVRPLHGK GGRNAHGRIT TRHKGGGHKR AYRVIDFRRH DKDGVNAKVA HIEYDPNRT ANIALLHYLD GEKRYIIAPQ GLKQGDVIES GANADIKPGN NLPLRNIPAG TVIHAVELRP GGGAKLARSA G VSIQLLGK EGTYAALRMP SGEIRRVDVR CRATVGEVGN AEQSNINWGK AGRMRWKGKR PTVRGVVMNP VDHPHGGGEG KT SGGRHPV SPWGKPEGRT RKPNKPSDKL IVRRRRTGKK R

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 23.011393 KDa
SequenceString: MARKGILGTK LGMTQVFDEN NKVVPVTVVK AGPNVVTRIR TTERDGYSAV QLAYGEISPR KVIKPVAGQF AAAGVNPRRH VAELRLDDE AAVAEYEVGQ ELTAEIFSDG AYVDVTGTSK GKGFAGTMKR HGFRGQGAAH GAQAVHRRPG SIGGCATPGR V FKGTRMSG ...String:
MARKGILGTK LGMTQVFDEN NKVVPVTVVK AGPNVVTRIR TTERDGYSAV QLAYGEISPR KVIKPVAGQF AAAGVNPRRH VAELRLDDE AAVAEYEVGQ ELTAEIFSDG AYVDVTGTSK GKGFAGTMKR HGFRGQGAAH GAQAVHRRPG SIGGCATPGR V FKGTRMSG RMGNDRVTTQ NLKVHKVDAE NGVLLIKGAI PGRNGGLVVV RSAIKRGEK

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 22.789754 KDa
SequenceString: TLKVDVKTPA GKTDGSVELP AELFDVEPNI ALMHQVVTAQ LAAKRQGTHS TKTRGEVSGG GKKPYRQKGT GRARQGSTRA PQFTGGGTV HGPKPRDYSQ RTPKKMIAAA LRGALSDRAR NDRIHAVTEL VEGQTPSTKS AKTFLGTLTE NKKVLVVIGR T DEVGAKSV ...String:
TLKVDVKTPA GKTDGSVELP AELFDVEPNI ALMHQVVTAQ LAAKRQGTHS TKTRGEVSGG GKKPYRQKGT GRARQGSTRA PQFTGGGTV HGPKPRDYSQ RTPKKMIAAA LRGALSDRAR NDRIHAVTEL VEGQTPSTKS AKTFLGTLTE NKKVLVVIGR T DEVGAKSV RNLPGVHVIS PDQLNTYDVL NADDVVFSVE ALNAYISANS KEGASV

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 21.021129 KDa
SequenceString:
TTTEKALPRL KQRYREEIRE ALQQEFNYAN VMQIPGVVKV VVNMGVGDAA RDAKLINGAI NDLALITGQK PEVRRARKSI AQFKLREGM PIGARVTLRG DRMWEFLDRL ISIALPRIRD FRGLSPKQFD GTGNYTFGLN EQSMFHEIDV DSIDRPRGMD I TVVTTATN DAEGRALLRA LGFPFKEN

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 19.483361 KDa
SequenceString:
MSRIGKQPVP VPSGVDVTIN GQNLSVKGPK GTLTLDVAEP ISVSRAEDGA IVVTRPDDER RSRSLHGLSR TLIANLVTGV TEGYTQKME IFGVGYRVQL KGQNLEFALG YSHPVLIEAP EGITFAVESP TKFSVSGIDK QKVGQISAVI RRLRRPDPYK G KGVRYEGE QIRRKVGKTG K

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #8: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 15.949253 KDa
SequenceString:
MKLILTAEVE HLGAAGDTVE VKDGYGRNYL LPRGLAIVAS RGAERQAEEI RRARESKVIR DIEHANELKT ALEGLGDVTL SVNAAGDTG KLFGSVTAAD VVNAIKKAGG PNLDKRTVQL AKAHIKSVGT HPVTVKLHTG VEAKVSLNVV AQ

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #9: 50S ribosomal protein L10

MacromoleculeName: 50S ribosomal protein L10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 18.035736 KDa
SequenceString:
MAKADKATAV ADIAEQFKAS TATVVTEYRG LTVANLAELR RALGDSATYT VAKNTLVKRA ASEAGIEGLD ELFAGPTAIA FVKGEAVDA AKAIKKFAKD NKALVIKGGY MDGKALSVAD VEKIADLESR EVLLAKLAGA MKGNLSKAAG LFNAPASQVA R LAAALQEK KAGEEAA

UniProtKB: Large ribosomal subunit protein uL10

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Macromolecule #10: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 15.023374 KDa
SequenceString:
MAPKKKVAGL IKLQIQAGQA NPAPPVGPAL GQHGVNIMEF CKAYNAATES QRGNVIPVEI TVYEDRSFTF ALKTPPAAKL LLKAAGVQK GSGEPHKTKV AKVTWDQVRE IAETKKADLN ANDIDAAAKI IAGTARSMGI TVE

UniProtKB: Large ribosomal subunit protein uL11

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Macromolecule #11: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 16.015493 KDa
SequenceString:
PTYTPKAGDT TRSWYVIDAS DVVLGRLASA AATLLRGKHK PTFTPNVDGG DFVIVINADK IAVSGDKLTK KFAYRHSGYP GGLRKRTIG ELLEKHPTRV VENAIIGMLP HNKLGRQIQK KLKVYAGPDH PHAAQQPIPF EIKQVAQ

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #12: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 13.400625 KDa
SequenceString:
MIQQESRLKV ADNTGAKEIL CIRVLGGSSR RYAGIGDVIV ATVKDAIPGG NVKRGDVVKA VVVRTVKERR RADGSYIKFD ENAAVIIKN DNDPRGTRIF GPVGRELREK KFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

+
Macromolecule #13: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 15.602244 KDa
SequenceString:
MSVIKLHDLK PAPGEKKAKT RVGRGEGSKG KTAGRGTKGT KARKNVPVMF EGGQMPIHMR LPKLKGFKNR FRTEYQVVNV GDINKAFPQ GGTVGVDELV AKGLVRKNSL VKVLGDGKLT VKVDVTANKF SGSAREAITA AGGSATEL

UniProtKB: Large ribosomal subunit protein uL15

+
Macromolecule #14: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 15.77424 KDa
SequenceString:
MLIPRKVKHR KQHHPEQRGI ASGGTSVSFG DYGIQALEHA YITNRQIESA RIAINRHIKR GGKVWINIFP DRPLTKKPAE TRMGSGKGS PEWWVANVKP GRVLFELSYP DEKTARDALT RAIHKLPIKA RIVTREEQF

UniProtKB: Large ribosomal subunit protein uL16

+
Macromolecule #15: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 21.892246 KDa
SequenceString: MPKPTKGPRL GGSSSHQSAL LANLATSLFE HGRIKTTEPK ARALRPYAEK LITHAKKGAL HNRREVMKKI RDKDVVHTLF AEIGPFYAD RNGGYTRIIK VENRKGDNAP MAVIELVREK TVTDEANRAR RAAASQAKAD ERADEKADEK AEETVEETTE A PAEESTEA ...String:
MPKPTKGPRL GGSSSHQSAL LANLATSLFE HGRIKTTEPK ARALRPYAEK LITHAKKGAL HNRREVMKKI RDKDVVHTLF AEIGPFYAD RNGGYTRIIK VENRKGDNAP MAVIELVREK TVTDEANRAR RAAASQAKAD ERADEKADEK AEETVEETTE A PAEESTEA AAEETVEETT EAPAEESTEA AEESEAKDDT K

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #16: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 13.580531 KDa
SequenceString:
AHKPVGQNIS EVRRNARLRR HARLRKKVAG TAEVPRLVVN RSARHIHVQL VNDLNGTTLA AASSIEADVR AIDGDKKAHS VRVGQLIAE RAKAAGVETV VFDRGGYTYG GRIAALADAA REAGLKF

UniProtKB: Large ribosomal subunit protein uL18

+
Macromolecule #17: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 12.892806 KDa
SequenceString:
MNTLDFVDQA SLRDDIPTFS PGDTVNVHVK VIEGSKERIQ VFKGVVIRRQ GGGISETFTV RKESYGVGVE RTFPVHSPNI DHIDVLTRG DVRRAKLYYL RELRGKKAKI KEKR

UniProtKB: Large ribosomal subunit protein bL19

+
Macromolecule #18: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 14.494693 KDa
SequenceString:
MARVKRALNA QKKRRTVLKA SKGYRGQRSR LYRKAKEQQL HSLTYAYRDR RARKGEFRKL WISRINAAAR ANDITYNRLI QGLKAAGVE VDRKNLAELA VSDPAAFTAL VDVARAALPE DVNAPSGEAA

UniProtKB: Large ribosomal subunit protein bL20

+
Macromolecule #19: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 10.924698 KDa
SequenceString:
ATYAIVKTGG KQYKVAAGDV VKVEKLDSEP GASVSLPVAL VVDGANVTSK ADDLAKVAVT AEVLEHTKGP KIRIHKFKNK TGYHKRQGH RQQLTVLKVT GIK

UniProtKB: Large ribosomal subunit protein bL21

+
Macromolecule #20: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 16.222404 KDa
SequenceString:
STVTEFPSAT AKARYVRVSA TKARRVIDLV RGKSVEEALD ILRWAPQAAS EPVAKVIASA AANAQNNEGL DPSTLVVATV YADEGPTAK RIRPRAQGRA FRIRKRTSHI TVIVESRPPK QKGASAASAR SRRAQGSKAA ATKKSAETKE GSE

UniProtKB: Large ribosomal subunit protein uL22

+
Macromolecule #21: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 10.916641 KDa
SequenceString:
ATITDPRDII LAPVISEKSY GLIEDNVYTF VVHPDSNKTQ IKIAIEKIFD VKVDSVNTAN RQGKRKRTRT GFGKRKSTKR AIVKLAAGS KPIDLFGAPA

UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #22: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 11.228946 KDa
SequenceString:
MKVHKGDTVL VISGKDKGAK GKVLVAYPDR NKVLVEGVNR IKKHTAVSAN ERGASSGGIV TQEAPIHVSN VMVVDSDGKP TRVGYRIDD ETGKKVRIAK TNGKDI

UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #23: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 22.571078 KDa
SequenceString: MAKTANIPNK LTANVRTRTG KGASRQARRD GKVPAVLYGH GTDPQHLELN ARDFAAVLRS HGTNAILTLD IEGTEQLALT KALDVHPIR RNIQHADLLV VQRGEKVTVE VTVLVEGDAT PGTLVTQDAN TIEIEAEALS IPEQLTVSVE GVEAGTQITA G QISLPEGV ...String:
MAKTANIPNK LTANVRTRTG KGASRQARRD GKVPAVLYGH GTDPQHLELN ARDFAAVLRS HGTNAILTLD IEGTEQLALT KALDVHPIR RNIQHADLLV VQRGEKVTVE VTVLVEGDAT PGTLVTQDAN TIEIEAEALS IPEQLTVSVE GVEAGTQITA G QISLPEGV NLISDPELLV VNVVEAPSAE ALEEEGAGET GAAEAPAEEA PAEEPAE

UniProtKB: Large ribosomal subunit protein bL25

+
Macromolecule #24: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 9.249582 KDa
SequenceString:
MAHKKGASSS RNGRDSAAQR LGVKRFGGQV VKAGEILVRQ RGTHFHPGVN VGRGGDDTLF ALAPGAVEFG AKRGRKTVNI VPVARPEA

UniProtKB: Large ribosomal subunit protein bL27

+
Macromolecule #25: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 8.790895 KDa
SequenceString:
MAVGTTPGEL RELTDDELKD KLRESKEELF NLRFQMATGQ LSNNRRLRTV RQEIARVYTV LRERELGLAS GPAGEES

UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #26: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 7.022147 KDa
SequenceString:
MAELKITQVR STIGARWKQR ESLRTLGLKK IRQSVVREDN AQTRGLINTV HHLVEVEEVG K

UniProtKB: Large ribosomal subunit protein uL30

+
Macromolecule #27: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 6.467719 KDa
SequenceString:
MAVPKRRMSR ANTRSRRAQW KAEAPGLVTV SVAGQQRKVP RRLLKAARLG LVDLDKR

UniProtKB: Large ribosomal subunit protein bL32

+
Macromolecule #28: 50S ribosomal protein L33 2

MacromoleculeName: 50S ribosomal protein L33 2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 6.61564 KDa
SequenceString:
MARNEIRPIV KLRSTAGTGY TYVTRKNRRN DPDRIVLRKY DPVLRRHVEF REER

UniProtKB: Large ribosomal subunit protein bL33B

+
Macromolecule #29: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 5.52258 KDa
SequenceString:
MAKGKRTFQP NNRRRARVHG FRLRMRTRAG RAIVANRRSK GRRALTA

UniProtKB: Large ribosomal subunit protein bL34

+
Macromolecule #30: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 7.298505 KDa
SequenceString:
MPKAKTHSGA SKRFRRTGTG KIVRQKANRR HLLEHKPTKR TRRLDGRTTV SAADNSRINK LLNG

UniProtKB: Large ribosomal subunit protein bL35

+
Macromolecule #31: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 4.341277 KDa
SequenceString:
MKVNPSVKPI CDKCRVIRRH GRVMVICSDP RHKQRQG

UniProtKB: Large ribosomal subunit protein bL36

+
Macromolecule #32: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 9.141198 KDa
SequenceString:
MKPGIHPDYH PVVFQDAATG AQFLTRSTAT STRTIEWPTP SGPKTYPLIV VDVTSDSHPF WTGSARHIDS AGQVEKFRRR YG

UniProtKB: 50S ribosomal protein L31

+
Macromolecule #33: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis str. MC2 155 (bacteria) / Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 8.925234 KDa
SequenceString:
MSAHCQVTGR GPGFGNSVSH SHRRTPRRWD PNIQNKTYYL PSEGRRIRLR VSAKGIKVID RDGIEAVVAR MRARGEKY

+
Macromolecule #34: Uncharacterized protein

MacromoleculeName: Uncharacterized protein / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria)
Strain: ATCC 700084 / mc(2)155
Molecular weightTheoretical: 2.710179 KDa
SequenceString:
AKRGRKKRDR KHSKANHGKR PNA

UniProtKB: Uncharacterized protein

-
Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.5
GridSupport film - Material: CARBON / Support film - topology: CONTINUOUS / Details: unspecified
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK I

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 67.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 205510
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5o61

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.01)
Final 3D classificationNumber classes: 10 / Avg.num./class: 66840 / Software - Name: RELION (ver. 2.01)
Software - details: Relion used for image processing and Refinement
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.01)
Final reconstructionApplied symmetry - Point group: C2 (2 fold cyclic) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.42 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.0) / Number images used: 66840
FSC plot (resolution estimation)

-
Atomic model buiding 1

RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-6dzp:
Cryo-EM Structure of Mycobacterium smegmatis C(minus) 50S ribosomal subunit

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