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- EMDB-74149: Mitochondrial Hsp60 from Acanthamoeba castellanii -

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Basic information

Entry
Database: EMDB / ID: EMD-74149
TitleMitochondrial Hsp60 from Acanthamoeba castellanii
Map data
Sample
  • Complex: Acanthamoeba castellanii mitochondrial Hsp60
    • Protein or peptide: Chaperonin GroL, putative
Keywordsmitochondrial Hsp60 / cryoEM / CHAPERONE
Function / homology
Function and homology information


ATP-dependent protein folding chaperone / protein refolding / ATP binding
Similarity search - Function
Chaperonin Cpn60, conserved site / Chaperonins cpn60 signature. / Chaperonin Cpn60/GroEL / GroEL-like equatorial domain superfamily / TCP-1-like chaperonin intermediate domain superfamily / GroEL-like apical domain superfamily / TCP-1/cpn60 chaperonin family / Chaperonin Cpn60/GroEL/TCP-1 family
Similarity search - Domain/homology
Chaperonin GroL, putative
Similarity search - Component
Biological speciesAcanthamoeba castellanii (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.76 Å
AuthorsFry MY / Luce BE / Stefely JA / Samuelson J / Mootha VK / Chao LH
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Cell / Year: 2026
Title: Acanthamoeba ATP synthase structure reveals the TCA cycle is tethered to OXPHOS.
Authors: Michelle Y Fry / Bridget E Luce / Jonathan A Stefely / Michael Z Chen / Felicia G Deng / Tiana M Issa / / Sarah E Calvo / John Samuelson / Vamsi K Mootha / Luke H Chao /
Abstract: Oxidative phosphorylation (OXPHOS) is a key metabolic process that couples redox energy to ATP production. While some core OXPHOS complex subunits are found across all domains of life, many have ...Oxidative phosphorylation (OXPHOS) is a key metabolic process that couples redox energy to ATP production. While some core OXPHOS complex subunits are found across all domains of life, many have diverged or expanded across evolution-as seen in the protozoan pathogen Acanthamoeba castellanii. By integrating cryo-electron microscopy of unenriched mitochondrial lysate with mass spectrometry proteomics, we resolved the structures of endogenous mitochondrial ATP synthase (complex V), Hsp60, and respiratory complex III from Acanthamoeba. We capture Acanthamoeba ATP synthase in an IF1-inhibited state and reveal how Acanthamoeba-specific subunits and extensions stabilize the molecular machine, which includes a β subunit extension that interfaces with the peripheral stalk. Additionally, we characterize an active malate dehydrogenase (MDH) dimer structurally integrated within the ATP synthase peripheral stalk, thus revealing a direct protein tether between OXPHOS and the tricarboxylic acid cycle. Together, these findings provide structural insight into lineage-specific adaptations in Acanthamoeba that may tune protozoan metabolism.
History
DepositionDec 2, 2025-
Header (metadata) releaseOct 14, 2026-
Map releaseOct 14, 2026-
UpdateOct 14, 2026-
Current statusOct 14, 2026Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_74149.png

Downloads & links

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Map

FileDownload / File: emd_74149.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 400 pix.
= 328.8 Å		0.82 Å/pix.
x 400 pix.
= 328.8 Å		0.82 Å/pix.
x 400 pix.
= 328.8 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.822 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.1788686 - 0.3581552
Average (Standard dev.)0.0019284165 (±0.02016597)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 328.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_74149_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_74149_half_map_2.map
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Sample components

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Entire : Acanthamoeba castellanii mitochondrial Hsp60

EntireName: Acanthamoeba castellanii mitochondrial Hsp60
Components
  • Complex: Acanthamoeba castellanii mitochondrial Hsp60
    • Protein or peptide: Chaperonin GroL, putative

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Supramolecule #1: Acanthamoeba castellanii mitochondrial Hsp60

SupramoleculeName: Acanthamoeba castellanii mitochondrial Hsp60 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Acanthamoeba castellanii (eukaryote) / Strain: Neff
Molecular weightTheoretical: 812 KDa

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Macromolecule #1: Chaperonin GroL, putative

MacromoleculeName: Chaperonin GroL, putative / type: protein_or_peptide / ID: 1 / Details: ACANB13112 / Number of copies: 14 / Enantiomer: LEVO
Source (natural)Organism: Acanthamoeba castellanii (eukaryote) / Strain: Neff
Molecular weightTheoretical: 60.910426 KDa
SequenceString: MLRTAAPHAR VAVRGSSRSA SSLRLYASVA GKEIKFGSEA RSLMLRGVDS LTDAVQTTLG PKGRNVVLEQ SFGAPKITKD GVTVAKHIE FKDKYMNMGA QLVRGVASKA NDVAGDGTTT ATVLARAIFS EGTKVVAAGM NPMDVKRGVD QAVSIVVGEL K KLAKKVTT ...String:
MLRTAAPHAR VAVRGSSRSA SSLRLYASVA GKEIKFGSEA RSLMLRGVDS LTDAVQTTLG PKGRNVVLEQ SFGAPKITKD GVTVAKHIE FKDKYMNMGA QLVRGVASKA NDVAGDGTTT ATVLARAIFS EGTKVVAAGM NPMDVKRGVD QAVSIVVGEL K KLAKKVTT TEEIRQVATL SANSDDSIGS LIATAMEKVG SQGVITVTDG KTLENEVEVI EGMKFDQGYI SRYFVTDPKT QK CEYEDAL FLLTDGKISS IHSLLPCLEE VSREHKKLVI ISDNVEGEAL ATLIINKMRG LPVVAVKAPG FGDNRKNNLQ DIA VLTGAT VVSEEMGLKL ENFERSWLGS SKKVIISSDD TIIMDGGGNS ESIKERCEQI TEALQRTTSS YEQEKLRERL AKLS SGVAV LKVGGATEVE VSERKDRITD ALSATRAAVE EGIVPGGGVA LLHASKALEG VTGANADQTS GIQLVARAIR IPART IATN AGKEGGVIVE KILQSTDAHW GYNAQTDEYG DMFKAGIIDP LKVVRTAIDA AASVSSLMTT TEAIVVELPK ESSMPA APG MGGMGGMGGD MF

UniProtKB: Chaperonin GroL, putative

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2.25 mg/mL
BufferpH: 7.9
Component:
ConcentrationName
50.0 mMHEPES
300.0 mMsodium chloride
4.0 mMmagnesium chloride
20.0 uMCalcium Chloride
1.0 mMDithiothreitol
0.5 % (w/v)DDM
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 277.14 K / Instrument: FEI VITROBOT MARK IV
DetailsSample was not purified. Instead it was solved from imaging mitochondrial lysate.

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Electron microscopy

MicroscopeTFS KRIOS
Specialist opticsEnergy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 0.99 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: DARK FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC (ver. v.4.7.0) / Software - details: Patch CTF estimation / Type: NONE
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.76 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 1154
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: cryoSPARC / Software - details: ab initio reconstruction
Final angle assignmentType: PROJECTION MATCHING

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