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- EMDB-7341: Conformation of methylated GGQ in the peptidyl transferase center... -

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Basic information

Entry
Database: EMDB / ID: EMD-7341
TitleConformation of methylated GGQ in the peptidyl transferase center during translation termination
Map datanon-stop termination complex
Sample
  • Complex: nonstop ribosomal complex with ArfA/RF2
    • Complex: 50S subunit
      • Complex: 50S ribosomal proteins
        • Organelle or cellular component: 50S ribosomal protein L2
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L3
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L4
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L5
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L6
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L9
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L10
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L11
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L13
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L14
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L15
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L16
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L17
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L18
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L19
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L20
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L21
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L22
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L23
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L24
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L25
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L27
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L28
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L29
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L30
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L31
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L32
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L33
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L34
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L35
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 50S ribosomal protein L36
          • Protein or peptide: x 1 types
      • Organelle or cellular component: 23S rRNA
        • RNA: x 1 types
      • Organelle or cellular component: 5S rRNA
        • RNA: x 1 types
    • Complex: 30S subunit
      • Complex: 30S ribosomal proteins
        • Organelle or cellular component: 30S ribosomal protein S2
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S3
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S4
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S5
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S6
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S7
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S8
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S9
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S10
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S11
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S12
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S13
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S14
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S15
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S16
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S17
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S18
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S19
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S20
          • Protein or peptide: x 1 types
        • Organelle or cellular component: 30S ribosomal protein S21
          • Protein or peptide: x 1 types
      • Organelle or cellular component: 16S rRNA
        • RNA: x 1 types
    • Organelle or cellular component: Peptide chain release factor 2
      • Protein or peptide: x 1 types
    • Organelle or cellular component: Alternative ribosome-rescue factor A
      • Protein or peptide: x 1 types
    • Organelle or cellular component: E-site or P-site tRNA fMet
      • RNA: x 1 types
    • Organelle or cellular component: nonstop mRNA
      • RNA: x 1 types
  • Ligand: x 3 types
Function / homology
Function and homology information


translation release factor activity, codon specific / negative regulation of cytoplasmic translational initiation / stringent response / ribosomal large subunit binding / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation ...translation release factor activity, codon specific / negative regulation of cytoplasmic translational initiation / stringent response / ribosomal large subunit binding / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / four-way junction DNA binding / translational termination / translation repressor activity / negative regulation of translational initiation / regulation of mRNA stability / mRNA regulatory element binding translation repressor activity / rescue of stalled ribosome / ribosome assembly / assembly of large subunit precursor of preribosome / positive regulation of RNA splicing / transcription elongation factor complex / cytosolic ribosome assembly / regulation of DNA-templated transcription elongation / DNA endonuclease activity / response to reactive oxygen species / transcription antitermination / regulation of cell growth / translational initiation / DNA-templated transcription termination / maintenance of translational fidelity / response to radiation / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / large ribosomal subunit / ribosome biogenesis / ribosome binding / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / transferase activity / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / molecular adaptor activity / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / viral translational frameshifting / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm
Similarity search - Function
Alternative ribosome-rescue factor A / Alternative ribosome-rescue factor A / Peptide chain release factor 2 / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain ...Alternative ribosome-rescue factor A / Alternative ribosome-rescue factor A / Peptide chain release factor 2 / Peptide chain release factor / PCRF domain / PCRF / Peptide chain release factor class I superfamily / Prokaryotic-type class I peptide chain release factors signature. / Peptide chain release factor class I / RF-1 domain / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / Ribosomal protein L10 / : / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / : / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L10-like domain superfamily / Ribosomal protein L16 signature 1. / Ribosomal protein L10P / Ribosomal protein L10 / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11, RNA binding domain / Ribosomal protein L11/L12 / Ribosomal protein L36 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / : / : / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L20 signature. / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S4, bacterial-type
Similarity search - Domain/homology
Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Peptide chain release factor RF2 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 ...Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Peptide chain release factor RF2 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL18 / Alternative ribosome-rescue factor A / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL25
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.24 Å
AuthorsZeng F / Jin H
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM120552 United States
CitationJournal: Sci Rep / Year: 2018
Title: Conformation of methylated GGQ in the Peptidyl Transferase Center during Translation Termination.
Authors: Fuxing Zeng / Hong Jin /
Abstract: The universally conserved Gly-Gly-Gln (GGQ) tripeptide in release factors or release factor-like surveillance proteins is required to catalyze the release of nascent peptide in the ribosome. The ...The universally conserved Gly-Gly-Gln (GGQ) tripeptide in release factors or release factor-like surveillance proteins is required to catalyze the release of nascent peptide in the ribosome. The glutamine of the GGQ is methylated post-translationally at the N position in vivo; this covalent modification is essential for optimal cell growth and efficient translation termination. However, the precise conformation of the methylated-GGQ tripeptide in the ribosome remains unknown. Using cryoEM and X-ray crystallography, we report the conformation of methylated-GGQ in the peptidyl transferase center of the ribosome during canonical translational termination and co-translation quality control. It has been suggested that the GGQ motif arose independently through convergent evolution among otherwise unrelated proteins that catalyze peptide release. The requirement for this tripeptide in the highly conserved peptidyl transferase center suggests that the conformation reported here is likely shared during termination of protein synthesis in all domains of life.
History
DepositionJan 12, 2018-
Header (metadata) releaseFeb 21, 2018-
Map releaseFeb 21, 2018-
UpdateJan 8, 2020-
Current statusJan 8, 2020Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.06
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6c4i
  • Surface level: 0.06
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_7341.png

Downloads & links

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Map

FileDownload / File: emd_7341.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationnon-stop termination complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.2 Å/pix.
x 384 pix.
= 460.8 Å		1.2 Å/pix.
x 384 pix.
= 460.8 Å		1.2 Å/pix.
x 384 pix.
= 460.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.2 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.06 / Movie #1: 0.06
Minimum - Maximum-0.28294364 - 0.44829115
Average (Standard dev.)-0.00000127641 (±0.01619885)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 460.80002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.21.21.2
M x/y/z384384384
origin x/y/z0.0000.0000.000
length x/y/z460.800460.800460.800
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ281156
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS384384384
D min/max/mean-0.2830.448-0.000

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Supplemental data

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Half map: non-stop termination complex, half map #1

Fileemd_7341_half_map_1.map
Annotationnon-stop termination complex, half map #1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: non-stop termination complex, half map #2

Fileemd_7341_half_map_2.map
Annotationnon-stop termination complex, half map #2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : nonstop ribosomal complex with ArfA/RF2

EntireName: nonstop ribosomal complex with ArfA/RF2
Components
  • Complex: nonstop ribosomal complex with ArfA/RF2
    • Complex: 50S subunit
      • Complex: 50S ribosomal proteins
        • Organelle or cellular component: 50S ribosomal protein L2
          • Protein or peptide: 50S ribosomal protein L2
        • Organelle or cellular component: 50S ribosomal protein L3
          • Protein or peptide: 50S ribosomal protein L3
        • Organelle or cellular component: 50S ribosomal protein L4
          • Protein or peptide: 50S ribosomal protein L4
        • Organelle or cellular component: 50S ribosomal protein L5
          • Protein or peptide: 50S ribosomal protein L5
        • Organelle or cellular component: 50S ribosomal protein L6
          • Protein or peptide: 50S ribosomal protein L6
        • Organelle or cellular component: 50S ribosomal protein L9
          • Protein or peptide: 50S ribosomal protein L9
        • Organelle or cellular component: 50S ribosomal protein L10
          • Protein or peptide: 50S ribosomal protein L10
        • Organelle or cellular component: 50S ribosomal protein L11
          • Protein or peptide: 50S ribosomal protein L11
        • Organelle or cellular component: 50S ribosomal protein L13
          • Protein or peptide: 50S ribosomal protein L13
        • Organelle or cellular component: 50S ribosomal protein L14
          • Protein or peptide: 50S ribosomal protein L14
        • Organelle or cellular component: 50S ribosomal protein L15
          • Protein or peptide: 50S ribosomal protein L15
        • Organelle or cellular component: 50S ribosomal protein L16
          • Protein or peptide: 50S ribosomal protein L16
        • Organelle or cellular component: 50S ribosomal protein L17
          • Protein or peptide: 50S ribosomal protein L17
        • Organelle or cellular component: 50S ribosomal protein L18
          • Protein or peptide: 50S ribosomal protein L18
        • Organelle or cellular component: 50S ribosomal protein L19
          • Protein or peptide: 50S ribosomal protein L19
        • Organelle or cellular component: 50S ribosomal protein L20
          • Protein or peptide: 50S ribosomal protein L20
        • Organelle or cellular component: 50S ribosomal protein L21
          • Protein or peptide: 50S ribosomal protein L21
        • Organelle or cellular component: 50S ribosomal protein L22
          • Protein or peptide: 50S ribosomal protein L22
        • Organelle or cellular component: 50S ribosomal protein L23
          • Protein or peptide: 50S ribosomal protein L23
        • Organelle or cellular component: 50S ribosomal protein L24
          • Protein or peptide: 50S ribosomal protein L24
        • Organelle or cellular component: 50S ribosomal protein L25
          • Protein or peptide: 50S ribosomal protein L25
        • Organelle or cellular component: 50S ribosomal protein L27
          • Protein or peptide: 50S ribosomal protein L27
        • Organelle or cellular component: 50S ribosomal protein L28
          • Protein or peptide: 50S ribosomal protein L28
        • Organelle or cellular component: 50S ribosomal protein L29
          • Protein or peptide: 50S ribosomal protein L29
        • Organelle or cellular component: 50S ribosomal protein L30
          • Protein or peptide: 50S ribosomal protein L30
        • Organelle or cellular component: 50S ribosomal protein L31
          • Protein or peptide: 50S ribosomal protein L31
        • Organelle or cellular component: 50S ribosomal protein L32
          • Protein or peptide: 50S ribosomal protein L32
        • Organelle or cellular component: 50S ribosomal protein L33
          • Protein or peptide: 50S ribosomal protein L33
        • Organelle or cellular component: 50S ribosomal protein L34
          • Protein or peptide: 50S ribosomal protein L34
        • Organelle or cellular component: 50S ribosomal protein L35
          • Protein or peptide: 50S ribosomal protein L35
        • Organelle or cellular component: 50S ribosomal protein L36
          • Protein or peptide: 50S ribosomal protein L36
      • Organelle or cellular component: 23S rRNA
        • RNA: 23S rRNA
      • Organelle or cellular component: 5S rRNA
        • RNA: 5S rRNA
    • Complex: 30S subunit
      • Complex: 30S ribosomal proteins
        • Organelle or cellular component: 30S ribosomal protein S2
          • Protein or peptide: 30S ribosomal protein S2
        • Organelle or cellular component: 30S ribosomal protein S3
          • Protein or peptide: 30S ribosomal protein S3
        • Organelle or cellular component: 30S ribosomal protein S4
          • Protein or peptide: 30S ribosomal protein S4
        • Organelle or cellular component: 30S ribosomal protein S5
          • Protein or peptide: 30S ribosomal protein S5
        • Organelle or cellular component: 30S ribosomal protein S6
          • Protein or peptide: 30S ribosomal protein S6
        • Organelle or cellular component: 30S ribosomal protein S7
          • Protein or peptide: 30S ribosomal protein S7
        • Organelle or cellular component: 30S ribosomal protein S8
          • Protein or peptide: 30S ribosomal protein S8
        • Organelle or cellular component: 30S ribosomal protein S9
          • Protein or peptide: 30S ribosomal protein S9
        • Organelle or cellular component: 30S ribosomal protein S10
          • Protein or peptide: 30S ribosomal protein S10
        • Organelle or cellular component: 30S ribosomal protein S11
          • Protein or peptide: 30S ribosomal protein S11
        • Organelle or cellular component: 30S ribosomal protein S12
          • Protein or peptide: 30S ribosomal protein S12
        • Organelle or cellular component: 30S ribosomal protein S13
          • Protein or peptide: 30S ribosomal protein S13
        • Organelle or cellular component: 30S ribosomal protein S14
          • Protein or peptide: 30S ribosomal protein S14
        • Organelle or cellular component: 30S ribosomal protein S15
          • Protein or peptide: 30S ribosomal protein S15
        • Organelle or cellular component: 30S ribosomal protein S16
          • Protein or peptide: 30S ribosomal protein S16
        • Organelle or cellular component: 30S ribosomal protein S17
          • Protein or peptide: 30S ribosomal protein S17
        • Organelle or cellular component: 30S ribosomal protein S18
          • Protein or peptide: 30S ribosomal protein S18
        • Organelle or cellular component: 30S ribosomal protein S19
          • Protein or peptide: 30S ribosomal protein S19
        • Organelle or cellular component: 30S ribosomal protein S20
          • Protein or peptide: 30S ribosomal protein S20
        • Organelle or cellular component: 30S ribosomal protein S21
          • Protein or peptide: 30S ribosomal protein S21
      • Organelle or cellular component: 16S rRNA
        • RNA: 16S rRNA
    • Organelle or cellular component: Peptide chain release factor 2
      • Protein or peptide: Peptide chain release factor 2
    • Organelle or cellular component: Alternative ribosome-rescue factor A
      • Protein or peptide: Alternative ribosome-rescue factor A
    • Organelle or cellular component: E-site or P-site tRNA fMet
      • RNA: E-site or P-site tRNA fMet
    • Organelle or cellular component: nonstop mRNA
      • RNA: mRNA
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: water

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Supramolecule #1: nonstop ribosomal complex with ArfA/RF2

SupramoleculeName: nonstop ribosomal complex with ArfA/RF2 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#58

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Supramolecule #2: 50S subunit

SupramoleculeName: 50S subunit / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#33
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #3: 30S subunit

SupramoleculeName: 30S subunit / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #34-#54
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #11: 30S ribosomal proteins

SupramoleculeName: 30S ribosomal proteins / type: complex / ID: 11 / Parent: 3 / Macromolecule list: #35-#54
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #12: 50S ribosomal proteins

SupramoleculeName: 50S ribosomal proteins / type: complex / ID: 12 / Parent: 2 / Macromolecule list: #3-#33
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #4: Peptide chain release factor 2

SupramoleculeName: Peptide chain release factor 2 / type: organelle_or_cellular_component / ID: 4 / Parent: 1 / Macromolecule list: #55
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #5: Alternative ribosome-rescue factor A

SupramoleculeName: Alternative ribosome-rescue factor A / type: organelle_or_cellular_component / ID: 5 / Parent: 1 / Macromolecule list: #56
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #6: E-site or P-site tRNA fMet

SupramoleculeName: E-site or P-site tRNA fMet / type: organelle_or_cellular_component / ID: 6 / Parent: 1 / Macromolecule list: #57
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #7: nonstop mRNA

SupramoleculeName: nonstop mRNA / type: organelle_or_cellular_component / ID: 7 / Parent: 1 / Macromolecule list: #58
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #8: 23S rRNA

SupramoleculeName: 23S rRNA / type: organelle_or_cellular_component / ID: 8 / Parent: 2 / Macromolecule list: #1
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #9: 5S rRNA

SupramoleculeName: 5S rRNA / type: organelle_or_cellular_component / ID: 9 / Parent: 2 / Macromolecule list: #2
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #10: 16S rRNA

SupramoleculeName: 16S rRNA / type: organelle_or_cellular_component / ID: 10 / Parent: 3 / Macromolecule list: #34
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #13: 50S ribosomal protein L2

SupramoleculeName: 50S ribosomal protein L2 / type: organelle_or_cellular_component / ID: 13 / Parent: 12 / Macromolecule list: #3
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #14: 50S ribosomal protein L3

SupramoleculeName: 50S ribosomal protein L3 / type: organelle_or_cellular_component / ID: 14 / Parent: 12 / Macromolecule list: #4
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #15: 50S ribosomal protein L4

SupramoleculeName: 50S ribosomal protein L4 / type: organelle_or_cellular_component / ID: 15 / Parent: 12 / Macromolecule list: #5
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #16: 50S ribosomal protein L5

SupramoleculeName: 50S ribosomal protein L5 / type: organelle_or_cellular_component / ID: 16 / Parent: 12 / Macromolecule list: #6
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #17: 50S ribosomal protein L6

SupramoleculeName: 50S ribosomal protein L6 / type: organelle_or_cellular_component / ID: 17 / Parent: 12 / Macromolecule list: #7
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #18: 50S ribosomal protein L9

SupramoleculeName: 50S ribosomal protein L9 / type: organelle_or_cellular_component / ID: 18 / Parent: 12 / Macromolecule list: #8
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #19: 50S ribosomal protein L10

SupramoleculeName: 50S ribosomal protein L10 / type: organelle_or_cellular_component / ID: 19 / Parent: 12 / Macromolecule list: #9
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #20: 50S ribosomal protein L11

SupramoleculeName: 50S ribosomal protein L11 / type: organelle_or_cellular_component / ID: 20 / Parent: 12 / Macromolecule list: #10
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #21: 50S ribosomal protein L13

SupramoleculeName: 50S ribosomal protein L13 / type: organelle_or_cellular_component / ID: 21 / Parent: 12 / Macromolecule list: #11
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #22: 50S ribosomal protein L14

SupramoleculeName: 50S ribosomal protein L14 / type: organelle_or_cellular_component / ID: 22 / Parent: 12 / Macromolecule list: #12
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #23: 50S ribosomal protein L15

SupramoleculeName: 50S ribosomal protein L15 / type: organelle_or_cellular_component / ID: 23 / Parent: 12 / Macromolecule list: #13
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #24: 50S ribosomal protein L16

SupramoleculeName: 50S ribosomal protein L16 / type: organelle_or_cellular_component / ID: 24 / Parent: 12 / Macromolecule list: #14
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #25: 50S ribosomal protein L17

SupramoleculeName: 50S ribosomal protein L17 / type: organelle_or_cellular_component / ID: 25 / Parent: 12 / Macromolecule list: #15
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #26: 50S ribosomal protein L18

SupramoleculeName: 50S ribosomal protein L18 / type: organelle_or_cellular_component / ID: 26 / Parent: 12 / Macromolecule list: #16
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #27: 50S ribosomal protein L19

SupramoleculeName: 50S ribosomal protein L19 / type: organelle_or_cellular_component / ID: 27 / Parent: 12 / Macromolecule list: #17
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #28: 50S ribosomal protein L20

SupramoleculeName: 50S ribosomal protein L20 / type: organelle_or_cellular_component / ID: 28 / Parent: 12 / Macromolecule list: #18
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #29: 50S ribosomal protein L21

SupramoleculeName: 50S ribosomal protein L21 / type: organelle_or_cellular_component / ID: 29 / Parent: 12 / Macromolecule list: #19
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #30: 50S ribosomal protein L22

SupramoleculeName: 50S ribosomal protein L22 / type: organelle_or_cellular_component / ID: 30 / Parent: 12 / Macromolecule list: #20
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #31: 50S ribosomal protein L23

SupramoleculeName: 50S ribosomal protein L23 / type: organelle_or_cellular_component / ID: 31 / Parent: 12 / Macromolecule list: #21
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #32: 50S ribosomal protein L24

SupramoleculeName: 50S ribosomal protein L24 / type: organelle_or_cellular_component / ID: 32 / Parent: 12 / Macromolecule list: #22
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #33: 50S ribosomal protein L25

SupramoleculeName: 50S ribosomal protein L25 / type: organelle_or_cellular_component / ID: 33 / Parent: 12 / Macromolecule list: #23
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #34: 50S ribosomal protein L27

SupramoleculeName: 50S ribosomal protein L27 / type: organelle_or_cellular_component / ID: 34 / Parent: 12 / Macromolecule list: #24
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #35: 50S ribosomal protein L28

SupramoleculeName: 50S ribosomal protein L28 / type: organelle_or_cellular_component / ID: 35 / Parent: 12 / Macromolecule list: #25
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #36: 50S ribosomal protein L29

SupramoleculeName: 50S ribosomal protein L29 / type: organelle_or_cellular_component / ID: 36 / Parent: 12 / Macromolecule list: #26
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #37: 50S ribosomal protein L30

SupramoleculeName: 50S ribosomal protein L30 / type: organelle_or_cellular_component / ID: 37 / Parent: 12 / Macromolecule list: #27
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #38: 50S ribosomal protein L31

SupramoleculeName: 50S ribosomal protein L31 / type: organelle_or_cellular_component / ID: 38 / Parent: 12 / Macromolecule list: #28
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #39: 50S ribosomal protein L32

SupramoleculeName: 50S ribosomal protein L32 / type: organelle_or_cellular_component / ID: 39 / Parent: 12 / Macromolecule list: #29
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #40: 50S ribosomal protein L33

SupramoleculeName: 50S ribosomal protein L33 / type: organelle_or_cellular_component / ID: 40 / Parent: 12 / Macromolecule list: #30
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #41: 50S ribosomal protein L34

SupramoleculeName: 50S ribosomal protein L34 / type: organelle_or_cellular_component / ID: 41 / Parent: 12 / Macromolecule list: #31
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #42: 50S ribosomal protein L35

SupramoleculeName: 50S ribosomal protein L35 / type: organelle_or_cellular_component / ID: 42 / Parent: 12 / Macromolecule list: #32
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #43: 50S ribosomal protein L36

SupramoleculeName: 50S ribosomal protein L36 / type: organelle_or_cellular_component / ID: 43 / Parent: 12 / Macromolecule list: #33
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #44: 30S ribosomal protein S2

SupramoleculeName: 30S ribosomal protein S2 / type: organelle_or_cellular_component / ID: 44 / Parent: 11 / Macromolecule list: #35
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #45: 30S ribosomal protein S3

SupramoleculeName: 30S ribosomal protein S3 / type: organelle_or_cellular_component / ID: 45 / Parent: 11 / Macromolecule list: #36
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #46: 30S ribosomal protein S4

SupramoleculeName: 30S ribosomal protein S4 / type: organelle_or_cellular_component / ID: 46 / Parent: 11 / Macromolecule list: #37
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #47: 30S ribosomal protein S5

SupramoleculeName: 30S ribosomal protein S5 / type: organelle_or_cellular_component / ID: 47 / Parent: 11 / Macromolecule list: #38
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #48: 30S ribosomal protein S6

SupramoleculeName: 30S ribosomal protein S6 / type: organelle_or_cellular_component / ID: 48 / Parent: 11 / Macromolecule list: #39
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #49: 30S ribosomal protein S7

SupramoleculeName: 30S ribosomal protein S7 / type: organelle_or_cellular_component / ID: 49 / Parent: 11 / Macromolecule list: #40
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #50: 30S ribosomal protein S8

SupramoleculeName: 30S ribosomal protein S8 / type: organelle_or_cellular_component / ID: 50 / Parent: 11 / Macromolecule list: #41
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #51: 30S ribosomal protein S9

SupramoleculeName: 30S ribosomal protein S9 / type: organelle_or_cellular_component / ID: 51 / Parent: 11 / Macromolecule list: #42
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #52: 30S ribosomal protein S10

SupramoleculeName: 30S ribosomal protein S10 / type: organelle_or_cellular_component / ID: 52 / Parent: 11 / Macromolecule list: #43
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #53: 30S ribosomal protein S11

SupramoleculeName: 30S ribosomal protein S11 / type: organelle_or_cellular_component / ID: 53 / Parent: 11 / Macromolecule list: #44
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #54: 30S ribosomal protein S12

SupramoleculeName: 30S ribosomal protein S12 / type: organelle_or_cellular_component / ID: 54 / Parent: 11 / Macromolecule list: #45
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #55: 30S ribosomal protein S13

SupramoleculeName: 30S ribosomal protein S13 / type: organelle_or_cellular_component / ID: 55 / Parent: 11 / Macromolecule list: #46
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #56: 30S ribosomal protein S14

SupramoleculeName: 30S ribosomal protein S14 / type: organelle_or_cellular_component / ID: 56 / Parent: 11 / Macromolecule list: #47
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #57: 30S ribosomal protein S15

SupramoleculeName: 30S ribosomal protein S15 / type: organelle_or_cellular_component / ID: 57 / Parent: 11 / Macromolecule list: #48
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #58: 30S ribosomal protein S16

SupramoleculeName: 30S ribosomal protein S16 / type: organelle_or_cellular_component / ID: 58 / Parent: 11 / Macromolecule list: #49
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #59: 30S ribosomal protein S17

SupramoleculeName: 30S ribosomal protein S17 / type: organelle_or_cellular_component / ID: 59 / Parent: 11 / Macromolecule list: #50
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #60: 30S ribosomal protein S18

SupramoleculeName: 30S ribosomal protein S18 / type: organelle_or_cellular_component / ID: 60 / Parent: 11 / Macromolecule list: #51
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #61: 30S ribosomal protein S19

SupramoleculeName: 30S ribosomal protein S19 / type: organelle_or_cellular_component / ID: 61 / Parent: 11 / Macromolecule list: #52
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #62: 30S ribosomal protein S20

SupramoleculeName: 30S ribosomal protein S20 / type: organelle_or_cellular_component / ID: 62 / Parent: 11 / Macromolecule list: #53
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #63: 30S ribosomal protein S21

SupramoleculeName: 30S ribosomal protein S21 / type: organelle_or_cellular_component / ID: 63 / Parent: 11 / Macromolecule list: #54
Source (natural)Organism: Escherichia coli (E. coli)

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Macromolecule #1: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 941.832625 KDa
SequenceString: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG ...String:
GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG GUUAAUGAGG CGAACCGGGG GAACUGAAAC AUCUAAGUAC CCCGAGGAAA AGAAAUCAAC CGAGAUUCCC CC AGUAGCG GCGAGCGAAC GGGGAGCAGC CCAGAGCCUG AAUCAGUGUG UGUGUUAGUG GAAGCGUCUG GAAAGGCGCG CGA UACAGG GUGACAGCCC CGUACACAAA AAUGCACAUG CUGUGAGCUC GAUGAGUAGG GCGGGACACG UGGUAUCCUG UCUG AAUAU GGGGGGACCA UCCUCCAAGG CUAAAUACUC CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAA GAAC CCCGGCGAGG GGAGUGAAAA AGAACCUGAA ACCGUGUACG UACAAGCAGU GGGAGCACGC UUAGGCGUGU GACUGC GUA CCUUUUGUAU AAUGGGUCAG CGACUUAUAU UCUGUAGCAA GGUUAACCGA AUAGGGGAGC CGAAGGGAAA CCGAGUC UU AACUGGGCGU UAAGUUGCAG GGUAUAGACC CGAAACCCGG UGAUCUAGCC AUGGGCAGGU UGAAGGUUGG GUAACACU A ACUGGAGGAC CGAACCGACU AAU(1MG)(PSU)(5MU)GAAA AAUUAGCGGA UGACUUGUGG CUGGGGGUGA AAGGCCA AU CAAACCGGGA GAUAGCUGGU UCUCCCCGAA AGCUAUUUAG GUAGCGCCUC GUGAAUUCAU CUCCGGGGGU AGAGCACU G UUUCGGCAAG GGGGUCAACC CGACUUACCA ACCCGAUGCA AACUGCGAAU ACCGGAGAAU GUUAUCACGG GAGACACAC GGCGGG(PSU)GCU AACGUCCGUC GUGAAGAGGG AAACAACCCA GACCGCCAGC UAAGGUCCCA AAGUCAUGGU UAAGUG GGA AACGAUGUGG GAAGGCCCAG ACAGCCAGGA UGUUGGCUUA GAAGCAGCCA UCAUUUAAAG AAAGCGUAAU AGCUCAC UG GUCGAGUCGG CCUGCGCGGA AGAUGUAACG GGGCUAAACC AUGCACCGAA GCUGCGGCAG CGACGCUUAU GCGUUGUU G GGUAGGGGAG CGUUCUGUAA GCCUGCGAAG GUGUGCUGUG AGGCAUGCUG GAGGUAUCAG AAGUGCGAAU GCUGACAUA AGUAACGAUA AAGCGGGUGA AAAGCCCGCU CGCCGGAAGA CCAAGGGUUC CUGUCCAACG UUAAUCGGGG CAGGGUGAGU CGACCCCUA AGGCGAGGCC GAAAGGCGUA GUCGAUGGGA AACAGGUUAA UAUUCCUGUA CUUGGUGUUA CUGCGAAGGG G GGACGGAG AAGGCUAUGU UGGCCGGGCG ACGGUUGUCC CGGUUUAAGC GUGUAGGCUG GUUUUCCAGG CAAAUCCGGA AA AUCAAGG CUGAGGCGUG AUGACGAGGC ACUACGGUGC UGAAGCAACA AAUGCCCUGC UUCCAGGAAA AGCCUCUAAG CAU CAGGUA ACAUCAAAUC GUACCCCAAA CCGACAC(6MZ)GG UGGUCAGGUA GAGAAUACCA AGGCGCUUGA GAGAACUCGG GUGAAGGAA CUAGGCAAAA UGGUGCCGUA ACUUCGGGAG AAGGCACGCU GAUAUGUAGG UGAGGUCCCU CGCGGAUGGA G CUGAAAUC AGUCGAAGAU ACCAGCUGGC UGCAACUGUU UAUUAAAAAC ACAGCACUGU GCAAACACGA AAGUGGACGU AU ACGGUGU GACGCCU(2MG)CC CGGUGCCGGA AGGUUAAUUG AUGGGGUUAG CGCAAGCGAA GCUCUUGAUC GAAGCCCCG GUAAACGGCG GCCG(PSU)AAC(3TD)A (PSU)AACGGUCCU AAGGUAGCGA AA(5MU)UCCUUGU CGGGUAAGUU CCGA C(5MC)UGC ACGAAUGGCG UAAUGAUGGC CAGGCUGUCU CCACCCGAGA CUCAGUGAAA UUGAACUCGC UGUG(6MZ)AG AU GCAGUGUACC CGCGGCAAGA CGGAAAGACC CCGU(G7M)AACCU UUACUAUAGC UUGACACUGA ACAUUGAGCC UUGA UGUGU AGGAUAGGUG GGAGGCUUUG AAGUGUGGAC GCCAGUCUGC AUGGAGCCGA CCUUGAAAUA CCACCCUUUA AUGUU UGAU GUUCUAACGU UGACCCGUAA UCCGGGUUGC GGACAGUGUC UGGUGGGUAG UUUGACUG(OMG)G GCGGUCUCCU CC UAAAGAG UAACGGAGGA GCACGAAGGU UGGCUAAUCC UGGUCGGACA UCAGGAGGUU AGUGCAAUGG CAUAAGCCAG CUU GACUGC GAGCGUGACG GCGCGAGCAG GUGCGAAAGC AGGUCAUAGU GAUCCGGUGG UUCUGAAUGG AAGGGCCAUC GCUC AACGG AUAAAAGGUA CUCCG(2MG)GGAU AACAGGC(PSU)GA UACCGCCCAA GAGUUCAUAU CGACGGCGGU GUUUGGC A(OMC) CUCG(2MA)(PSU)GUCG GCUCAUCACA UCCUGGGGCU GAAGUAGGUC CCAAGGGUAU GGC(OMU)GUUCGC CA UUUAAAG UGGUACGCGA GC(PSU)GGGUUUA GAACGUCGUG AGACAGU(PSU)CG GUCCCUAUCU GCCGUGGGCG CUGGA GAAC UGAGGGGGGC UGCUCCUAGU ACGAGAGGAC CGGAGUGGAC GCAUCACUGG UGUUCGGGUU GUCAUGCCAA UGGCAC UGC CCGGUAGCUA AAUGCGGAAG AGAUAAGUGC UGAAAGCAUC UAAGCACGAA ACUUGCCCCG AGAUGAGUUC UCCCUGA CC CUUUAAGGGU CCUGAAGGAA CGUUGAAGAC GACGACGUUG AUAGGCCGGG UGUGUAAGCG CAGCGAUGCG UUGAGCUA A CCGGUACUAA UGAACCGUGA GGCUUAACCU U

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Macromolecule #2: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 38.79009 KDa
SequenceString:
UGCCUGGCGG CCGUAGCGCG GUGGUCCCAC CUGACCCCAU GCCGAACUCA GAAGUGAAAC GCCGUAGCGC CGAUGGUAGU GUGGGGUCU CCCCAUGCGA GAGUAGGGAA CUGCCAGGCA U

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Macromolecule #34: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 497.404969 KDa
SequenceString: AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGA AGCUUGCUU CUUUGCUGAC GAGUGGCGGA CGGGUGAGUA AUGUCUGGGA AACUGCCUGA UGGAGGGGGA UAACUACUGG A AACGGUAG ...String:
AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGA AGCUUGCUU CUUUGCUGAC GAGUGGCGGA CGGGUGAGUA AUGUCUGGGA AACUGCCUGA UGGAGGGGGA UAACUACUGG A AACGGUAG CUAAUACCGC AUAACGUCGC AAGACCAAAG AGGGGGACCU UCGGGCCUCU UGCCAUCGGA UGUGCCCAGA UG GGAUUAG CUAGUAGGUG GGGUAACGGC UCACCUAGGC GACGAUCCCU AGCUGGUCUG AGAGGAUGAC CAGCCACACU GGA ACUGAG ACACGGUCCA GACUCCUACG GGAGGCAGCA GUGGGGAAUA UUGCACAAUG GGCGCAAGCC UGAUGCAGCC AUGC CGCGU GUAUGAAGAA GGCCUUCGGG UUGUAAAGUA CUUUCAGCGG GGAGGAAGGG AGUAAAGUUA AUACCUUUGC UCAUU GACG UUACCCGCAG AAGAAGCACC GGCUAACUCC G(PSU)GCCAGCAG CC(G7M)CGGUAAU ACGGAGGGUG CAAGCGUU A AUCGGAAUUA CUGGGCGUAA AGCGCACGCA GGCGGUUUGU UAAGUCAGAU GUGAAAUCCC CGGGCUCAAC CUGGGAACU GCAUCUGAUA CUGGCAAGCU UGAGUCUCGU AGAGGGGGGU AGAAUUCCAG GUGUAGCGGU GAAAUGCGUA GAGAUCUGGA GGAAUACCG GUGGCGAAGG CGGCCCCCUG GACGAAGACU GACGCUCAGG UGCGAAAGCG UGGGGAGCAA ACAGGAUUAG A UACCCUGG UAGUCCACGC CGUAAACGAU GUCGACUUGG AGGUUGUGCC CUUGAGGCGU GGCUUCCGGA GCUAACGCGU UA AGUCGAC CGCCUGGGGA GUACGGCCGC AAGGUUAAAA CUCAAAUGAA UUGACGGGGG CCCGCACAAG CGGUGGAGCA UGU GGUUUA AUUCGAU(2MG)(5MC)A ACGCGAAGAA CCUUACCUGG UCUUGACAUC CACGGAAGUU UUCAGAGAUG AGAAUG UGC CUUCGGGAAC CGUGAGACAG GUGCUGCAUG GCUGUCGUCA GCUCGUGUUG UGAAAUGUUG GGUUAAGUCC CGCAACG AG CGCAACCCUU AUCCUUUGUU GCCAGCGGUC CGGCCGGGAA CUCAAAGGAG ACUGCCAGUG AUAAACUGGA GGAAGGUG G GGAUGACGUC AAGUCAUCAU G(2MG)CCCUUACG ACCAGGGCUA CACACGUGCU ACAAUGGCGC AUACAAAGAG AAGCG ACCU CGCGAGAGCA AGCGGACCUC AUAAAGUGCG UCGUAGUCCG GAUUGGAGUC UGCAACUCGA CUCCAUGAAG UCGGAA UCG CUAGUAAUCG UGGAUCAGAA UGCCACGGUG AAUACGUUCC CGGGCCUUGU ACACACCG(4OC)C CGU(5MC)ACACC AUGGGAGUGG GUUGCAAAAG AAGUAGGUAG CUUAACCUUC GGGAGGGCGC UUACCACUUU GUGAUUCAUG ACUGGGGUGA AGUCG(UR3)AAC AAGGUAACCG UAGG(2MG)G(MA6)(MA6)CC UGCGGUUGGA UCA

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Macromolecule #57: E-site or P-site tRNA fMet

MacromoleculeName: E-site or P-site tRNA fMet / type: rna / ID: 57 / Number of copies: 2
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.802785 KDa
SequenceString:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

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Macromolecule #58: mRNA

MacromoleculeName: mRNA / type: rna / ID: 58 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 5.922644 KDa
SequenceString:
GGCAAGGAGG UAAAAAUG

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 29.923619 KDa
SequenceString: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD ...String:
MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD GAYVTLRLRS GEMRKVEADC RATLGEVGNA EHMLRVLGKA GAARWRGVRP TVRGTAMNPV DHPHGGGEGR NF GKHPVTP WGVQTKGKKT RSNKRTDKFI VRRRSK

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 22.277535 KDa
SequenceString: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE ...String:
MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE RVTVQSLDVV RVDAERNLLL VKGAVPGATG SDLIVKPAVK A

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 22.121566 KDa
SequenceString: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD ...String:
MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD VRDATGIDPV SLIAFDKVVM TADAVKQVEE MLA

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 20.073234 KDa
SequenceString:
AKLHDYYKDE VVKKLMTEFN YNSVMQVPRV EKITLNMGVG EAIADKKLLD NAAADLAAIS GQKPLITKAR KSVAGFKIRQ GYPIGCKVT LRGERMWEFF ERLITIAVPR IRDFRGLSAK SFDGRGNYSM GVREQIIFPE IDYDKVDRVR GLDITITTTA K SDEEGRAL LAAFDFPFR

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 18.932791 KDa
SequenceString:
MSRVAKAPVV VPAGVDVKIN GQVITIKGKN GELTRTLNDA VEVKHADNTL TFGPRDGYAD GWAQAGTARA LLNSMVIGVT EGFTKKLQL VGVGYRAAVK GNVINLSLGF SHPVDHQLPA GITAECPTQT EIVLKGADKQ VIGQVAADLR AYRRPEPYKG K GVRYADEV VRTKEAKKK

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Macromolecule #8: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 15.78902 KDa
SequenceString:
MQVILLDKVA NLGSLGDQVN VKAGYARNFL VPQGKAVPAT KKNIEFFEAR RAELEAKLAE VLAAANARAE KINALETVTI ASKAGDEGK LFGSIGTRDI ADAVTAAGVE VAKSEVRLPN GVLRTTGEHE VSFQVHSEVF AKVIVNVVAE

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Macromolecule #9: 50S ribosomal protein L10

MacromoleculeName: 50S ribosomal protein L10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 17.736596 KDa
SequenceString:
MALNLQDKQA IVAEVSEVAK GALSAVVADS RGVTVDKMTE LRKAGREAGV YMRVVRNTLL RRAVEGTPFE CLKDAFVGPT LIAYSMEHP GAAARLFKEF AKANAKFEVK AAAFEGELIP ASQIDRLATL PTYEEAIARL MATMKEASAG KLVRTLAAVR D AKEAA

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Macromolecule #10: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 14.894362 KDa
SequenceString:
MAKKVQAYVK LQVAAGMANP SPPVGPALGQ QGVNIMEFCK AFNAKTDSIE KGLPIPVVIT VYADRSFTFV TKTPPAAVLL KKAAGIKSG SGKPNKDKVG KISRAQLQEI AQTKAADMTG ADIEAMTRSI EGTARSMGLV VED

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Macromolecule #11: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 16.050606 KDa
SequenceString:
MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD KVAVTGNKRT DKVYYHHTGH IGGIKQATF EEMIARRPER VIEIAVKGML PKGPLGRAMF RKLKVYAGNE HNHAAQQPQV LDI

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Macromolecule #12: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 13.565067 KDa
SequenceString:
MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA VVVRTKKGVR RPDGSVIRFD GNACVLLNN NSEQPIGTRI FGPVTRELRS EKFMKIISLA PEVL

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Macromolecule #13: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 15.008471 KDa
SequenceString:
MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR LPKFGFTSRK AAITAEIRLS DLAKVEGGV VDLNTLKAAN IIGIQIEFAK VILAGEVTTP VTVRGLRVTK GARAAIEAAG GKIEE

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Macromolecule #14: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 15.312269 KDa
SequenceString:
MLQPKRTKFR KMHKGRNRGL AQGTDVSFGS FGLKAVGRGR LTARQIEAAR RAMTRAVKRQ GKIWIRVFPD KPITEKPLAV RMGKGKGNV EYWVALIQPG KVLYEMDGVP EELAREAFKL AAAKLPIKTT FVTKTVM

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Macromolecule #15: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 14.393657 KDa
SequenceString:
MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV ANRRLAFART RDNEIVAKLF NELGPRFAS RAGGYTRILK CGFRAGDNAP MAYIELVDRS EKAEAAAE

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Macromolecule #16: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 12.794668 KDa
SequenceString:
MDKKSARIRR ATRARRKLQE LGATRLVVHR TPRHIYAQVI APNGSEVLVA ASTVEKAIAE QLKYTGNKDA AAAVGKAVAE RALEKGIKD VSFDRSGFQY HGRVQALADA AREAGLQF

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Macromolecule #17: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 13.159278 KDa
SequenceString:
MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT VRKISNGEGV ERVFQTHSPV VDSISVKRR GAVRKAKLYY LRERTGKAAR IKERLN

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Macromolecule #18: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 13.528024 KDa
SequenceString:
MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVE IDRKILADIA VFDKVAFTAL VEKAKAALA

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Macromolecule #19: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 11.586374 KDa
SequenceString:
MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK AEVVAHGRGE KVKIVKFRRR KHYRKQQGH RQWFTDVKIT GISA

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Macromolecule #20: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 12.253359 KDa
SequenceString:
METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH NDGADIDDLK VTKIFVDEGP SMKRIMPRA KGRADRILKR TSHITVVVSD R

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Macromolecule #21: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 11.22216 KDa
SequenceString:
MIREERLLKV LRAPHVSEKA STAMEKSNTI VLKVAKDATK AEIKAAVQKL FEVEVEVVNT LVVKGKVKRH GQRIGRRSDW KKAYVTLKE GQNLDFVGGA E

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Macromolecule #22: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 11.33925 KDa
SequenceString:
MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE KEAAIQVSNV AIFNAATGKA DRVGFRFED GKKVRFFKSN SETIK

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Macromolecule #23: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 10.713465 KDa
SequenceString:
MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHI DFVRA

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Macromolecule #24: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 9.14654 KDa
SequenceString:
MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE

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Macromolecule #25: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 9.027551 KDa
SequenceString:
MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID KKGIDTVLAE LRARGEKY

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Macromolecule #26: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 7.286464 KDa
SequenceString:
MKAKELREKS VEELNTELLN LLREQFNLRM QAASGQLQQS HLLKQVRRDV ARVKTLLNEK AGA

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Macromolecule #27: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 6.55482 KDa
SequenceString:
MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE

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Macromolecule #28: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 7.887117 KDa
SequenceString:
MKKDIHPKYE EITASCSCGN VMKIRSTVGH DLNLDVCSKC HPFFTGKQRD VATGGRVDRF NKRFNIPGSK

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Macromolecule #29: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 6.463445 KDa
SequenceString:
MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR GRKVIAK

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Macromolecule #30: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 6.057179 KDa
SequenceString:
GIREKIKLVS SAGTGHFYTT TKNKRTKPEK LELKKFDPVV RQHVIYKEAK IK

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Macromolecule #31: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 5.397463 KDa
SequenceString:
MKRTFQPSVL KRNRSHGFRA RMATKNGRQV LARRRAKGRA RLTVSK

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Macromolecule #32: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 7.313032 KDa
SequenceString:
MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA CLPYA

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Macromolecule #33: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 4.37739 KDa
SequenceString:
MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG

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Macromolecule #35: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 26.78167 KDa
SequenceString: MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH ...String:
MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH EHIAIKEANN LGIPVFAIVD TNSDPDGVDF VIPGNDDAIR AVTLYLGAVA ATVREGRSQD LASQAEESFV EA E

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Macromolecule #36: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 26.031316 KDa
SequenceString: MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY ...String:
MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYNTSE AHTTYGVIGV KVWIFKGEIL GGMAAVEQPE KPAAQPKKQQ RKGRK

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Macromolecule #37: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 23.514199 KDa
SequenceString: MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP ...String:
MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP TWLEVDAGKM EGTFKRKPER SDLSADINEH LIVELYSK

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Macromolecule #38: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 17.629398 KDa
SequenceString:
MAHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI QKAMEKARRN MINVALNNGT LQHPVKGVH TGSRVFMQPA SEGTGIIAGG AMRAVLEVAG VHNVLAKAYG STNPINVVRA TIDGLENMNS PEMVAAKRGK S VEEILGK

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Macromolecule #39: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 15.727512 KDa
SequenceString:
MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP INKLHKAHYV LMNVEAPQEV IDELETTFRF NDAVIRSMV MRTKHAVTEA SPMVKAKDER RERRDDFANE TADDAEAGDS EEEEEE

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Macromolecule #40: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 20.055156 KDa
SequenceString:
MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE AFEVALENVR PTVEVKSRRV GGSTYQVPV EVRPVRRNAL AMRWIVEAAR KRGDKSMALR LANELSDAAE NKGTAVKKRE DVHRMAEANK AFAHYRWLSL R SFSHQAGA SSKQPALGYL N

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Macromolecule #41: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 14.146557 KDa
SequenceString:
MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK VEGDTKPELE LTLKYFQGKA VVESIQRVSR PGLRIYKRK DELPKVMAGL GIAVVSTSKG VMTDRAARQA GLGGEIICYV A

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Macromolecule #42: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 14.88627 KDa
SequenceString:
MAENQYYGTG RRKSSAARVF IKPGNGKIVI NQRSLEQYFG RETARMVVRQ PLELVDMVEK LDLYITVKGG GISGQAGAIR HGITRALME YDESLRSELR KAGFVTRDAR QVERKKVGLR KARRRPQFSK R

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Macromolecule #43: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 11.755597 KDa
SequenceString:
MQNQRIRIRL KAFDHRLIDQ ATAEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD ARDQYEIRTH LRLVDIVEPT EKTVDALMR LDLAAGVDVQ ISLG

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Macromolecule #44: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 13.870975 KDa
SequenceString:
MAKAPIRARK RVRKQVSDGV AHIHASFNNT IVTITDRQGN ALGWATAGGS GFRGSRKSTP FAAQVAAERC ADAVKEYGIK NLEVMVKGP GPGRESTIRA LNAAGFRITN ITDVTPIPHN GCRPPKKRRV

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Macromolecule #45: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 13.814249 KDa
SequenceString:
MATVNQLVRK PRARKVAKSN VPALEACPQK RGVCTRVYTT TPKKPNSALR KVCRVRLTNG FEVTSYIGGE GHNLQEHSVI LIRGGRVK(0TD) LPGVRYHTVR GALDCSGVKD RKQARSKYGV KRPKA

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Macromolecule #46: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 13.128467 KDa
SequenceString:
MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKPIKK

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Macromolecule #47: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 11.60656 KDa
SequenceString:
MAKQSMKARE VKRVALADKY FAKRAELKAI ISDVNASDED RWNAVLKLQT LPRDSSPSRQ RNRCRQTGRP HGFLRKFGLS RIKVREAAM RGEIPGLKKA SW

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Macromolecule #48: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 10.290816 KDa
SequenceString:
MSLSTEATAK IVSEFGRDAN DTGSTEVQVA LLTAQINHLQ GHFAEHKKDH HSRRGLLRMV SQRRKLLDYL KRKDVARYTQ LIERLGLRR

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Macromolecule #49: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 9.207572 KDa
SequenceString:
MVTIRLARHG AKKRPFYQVV VADSRNARNG RFIERVGFFN PIASEKEEGT RLDLDRIAHW VGQGATISDR VAALIKEVNK AA

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Macromolecule #50: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 9.724491 KDa
SequenceString:
MTDKIRTLQG RVVSDKMEKS IVVAIERFVK HPIYGKFIKR TTKLHVHDEN NECGIGDVVE IRECRPLSKT KSWTLVRVVE KAVL

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Macromolecule #51: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 7.734896 KDa
SequenceString:
FCRFTAEGVQ EIDYKDIATL KNYITESGKI VPSRITGTRA KYQRQLARAI KRARYLSLLP YTDRHQ

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Macromolecule #52: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 10.455355 KDa
SequenceString:
MPRSLKKGPF IDLHLLKKVE KAVESGDKKP LRTWSRRSTI FPNMIGLTIA VHNGRQHVPV FVTDEMVGHK LGEFAPTRTY RGHAADKKA KKK

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Macromolecule #53: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 9.708464 KDa
SequenceString:
MANIKSAKKR AIQSEKARKH NASRRSMMRT FIKKVYAAIE AGDKAAAQKA FNEMQPIVDR QAAKGLIHKN KAARHKANLT AQINKLA

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Macromolecule #54: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 8.524039 KDa
SequenceString:
MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL ARENARRTRL Y

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Macromolecule #55: Peptide chain release factor 2

MacromoleculeName: Peptide chain release factor 2 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 43.384945 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: AHHHHHHSAA LEVLFQGPGM FEINPVNNRI QDLTERSDVL RGYLDYDAKK ERLEEVNAEL EQPDVWNEPE RAQALGKERS SLEAVVDTL DQMKQGLEDV SGLLELAVEA DDEETFNEAV AELDALEEKL AQLEFRRMFS GEYDSADCYL DIQAGSGGTE A QDWASMLE ...String:
AHHHHHHSAA LEVLFQGPGM FEINPVNNRI QDLTERSDVL RGYLDYDAKK ERLEEVNAEL EQPDVWNEPE RAQALGKERS SLEAVVDTL DQMKQGLEDV SGLLELAVEA DDEETFNEAV AELDALEEKL AQLEFRRMFS GEYDSADCYL DIQAGSGGTE A QDWASMLE RMYLRWAESR GFKTEIIEES EGEVAGIKSV TIKISGDYAY GWLRTETGVH RLVRKSPFDS GGRRHTSFSS AF VYPEVDD DIDIEINPAD LRIDVYRTSG AGG(MEQ)HVNRTE SAVRITHIPT GIVTQCQNDR SQHKNKDQAM KQMKAKLYE LEMQKKNAEK QAMEDNKSDI GWGSQIRSYV LDDSRIKDLR TGVETRNTQA VLDGSLDQFI EASLKAGL

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Macromolecule #56: Alternative ribosome-rescue factor A

MacromoleculeName: Alternative ribosome-rescue factor A / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 6.513396 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
GSMSRYQHTK GQIKDNAIEA LLHDPLFRQR VEKNKKGKGS YMRKGKHGNR GNWEASG

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Macromolecule #59: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 59 / Number of copies: 317 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #60: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 60 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #61: water

MacromoleculeName: water / type: ligand / ID: 61 / Number of copies: 2 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4S4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
15.0 mMMg(CH3COO)2magnesium acetate
150.0 mMCH3CO2Kpotassium acetate
4.0 mMHOCH2CH2SHbeta-mercapthoethanol
2.0 mMC7H19N3spermidine
0.05 mMC10H26N4spermin
GridModel: C-flat-2/0.5 4C / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: Grids were blotted for 3.5 seconds..

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Electron microscopy

MicroscopeJEOL 3200FS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 20.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 83822 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Sample stageCooling holder cryogen: NITROGEN

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 4)
Startup modelType of model: EMDB MAP
EMDB ID:

8505

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.24 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2) / Number images used: 143372
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 2)
Final 3D classificationSoftware - Name: RELION (ver. 2)
FSC plot (resolution estimation)

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