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基本情報
登録情報 | ![]() | |||||||||
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タイトル | Cryo-EM structure of a TEF30-associated intermediate PSII core dimer complex, type I, from Chlamydomonas reinhardtii | |||||||||
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![]() | photosystem II / PSII repair / PSII assembly / PSII intermediate / TEF30 / PHOTOSYNTHESIS | |||||||||
機能・相同性 | ![]() oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / photosystem II / response to herbicide / chlorophyll binding / photosynthetic electron transport in photosystem II ...oxygen evolving activity / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / photosystem II / response to herbicide / chlorophyll binding / photosynthetic electron transport in photosystem II / photosynthesis, light reaction / chloroplast thylakoid membrane / phosphate ion binding / : / photosynthesis / electron transfer activity / protein stabilization / iron ion binding / heme binding / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.97 Å | |||||||||
![]() | Wang Y / Wang C / Li A / Liu Z | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii. 著者: Yidi Wang / Chenxi Wang / Anjie Li / Zhenfeng Liu / ![]() 要旨: During oxygenic photosynthesis, photosystem II (PSII) uses light energy for oxidizing water and reducing plastoquinone. It is susceptible to photodamage, and the damaged PSII is repaired through a ...During oxygenic photosynthesis, photosystem II (PSII) uses light energy for oxidizing water and reducing plastoquinone. It is susceptible to photodamage, and the damaged PSII is repaired through a sophisticated biological process assisted by numerous auxiliary proteins. Here we report the cryogenic electron microscopy structures of four PSII-repair complexes from Chlamydomonas reinhardtii associated with the Thylakoid Enriched Fraction 30 (TEF30, an orthologue of plant MET1) protein-namely, a TEF30-PSII core monomer (TEF30-C), two types of TEF30-PSII core dimers (types I and II, TEF30-C-I and TEF30-C-II) and a TEF30-CS-type PSII-LHCII supercomplex (TEF30-CS; S, strongly associated light-harvesting complex II trimer). TEF30 mediates the assembly of CP43 with the RC47 module by clamping on the stromal surfaces and prevents the premature association of peripheral antennae with PSII-C. In the transition from TEF30-C-I to TEF30-C-II, TEF30-CS and mature CS, one PSII core slides along the dimerization interface against the adjacent one by 22-35 Å, generating a zigzagged surface for accommodating the peripheral antennae. These results suggest that the PSII repair process undergoes multiple TEF30-mediated intermediate states to form intact PSII-LHCII supercomplexes. | |||||||||
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画像 | ![]() | 205.7 KB | ||
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文書・要旨 | ![]() | 1 MB | 表示 | ![]() |
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XML形式データ | ![]() | 18.3 KB | 表示 | |
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「今月の分子」の関連する項目 |
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ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.06 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : TEF30-associated PSII core dimer complex, type I
+超分子 #1: TEF30-associated PSII core dimer complex, type I
+分子 #1: Photosystem II protein D1
+分子 #2: Photosystem II CP47 reaction center protein
+分子 #3: Photosystem II CP43 reaction center protein
+分子 #4: Photosystem II D2 protein
+分子 #5: Cytochrome b559 subunit alpha
+分子 #6: Cytochrome b559 subunit beta
+分子 #7: PDZ domain-containing protein
+分子 #8: Photosystem II reaction center protein H
+分子 #9: Photosystem II reaction center protein I
+分子 #10: Photosystem II reaction center protein K
+分子 #11: Photosystem II reaction center protein L
+分子 #12: Photosystem II reaction center protein M
+分子 #13: Photosystem II reaction center protein T
+分子 #14: Photosystem II reaction center protein Psb30
+分子 #15: Photosystem II reaction center W protein, chloroplastic
+分子 #16: Chloroplast photosystem II subunit X
+分子 #17: Photosystem II reaction center protein Z
+分子 #18: FE (II) ION
+分子 #19: CHLOROPHYLL A
+分子 #20: PHEOPHYTIN A
+分子 #21: BETA-CAROTENE
+分子 #22: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+分子 #23: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+分子 #24: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+分子 #25: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+分子 #26: DODECYL-ALPHA-D-MALTOSIDE
+分子 #27: BICARBONATE ION
+分子 #28: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+分子 #29: PROTOPORPHYRIN IX CONTAINING FE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.2 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.8 µm / 最小 デフォーカス(公称値): 1.3 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |