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-Structure paper
| タイトル | Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii. |
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| ジャーナル・号・ページ | Nat Plants, Vol. 11, Issue 7, Page 1455-1469, Year 2025 |
| 掲載日 | 2025年6月27日 |
著者 | Yidi Wang / Chenxi Wang / Anjie Li / Zhenfeng Liu / ![]() |
| PubMed 要旨 | During oxygenic photosynthesis, photosystem II (PSII) uses light energy for oxidizing water and reducing plastoquinone. It is susceptible to photodamage, and the damaged PSII is repaired through a ...During oxygenic photosynthesis, photosystem II (PSII) uses light energy for oxidizing water and reducing plastoquinone. It is susceptible to photodamage, and the damaged PSII is repaired through a sophisticated biological process assisted by numerous auxiliary proteins. Here we report the cryogenic electron microscopy structures of four PSII-repair complexes from Chlamydomonas reinhardtii associated with the Thylakoid Enriched Fraction 30 (TEF30, an orthologue of plant MET1) protein-namely, a TEF30-PSII core monomer (TEF30-C), two types of TEF30-PSII core dimers (types I and II, TEF30-C-I and TEF30-C-II) and a TEF30-CS-type PSII-LHCII supercomplex (TEF30-CS; S, strongly associated light-harvesting complex II trimer). TEF30 mediates the assembly of CP43 with the RC47 module by clamping on the stromal surfaces and prevents the premature association of peripheral antennae with PSII-C. In the transition from TEF30-C-I to TEF30-C-II, TEF30-CS and mature CS, one PSII core slides along the dimerization interface against the adjacent one by 22-35 Å, generating a zigzagged surface for accommodating the peripheral antennae. These results suggest that the PSII repair process undergoes multiple TEF30-mediated intermediate states to form intact PSII-LHCII supercomplexes. |
リンク | Nat Plants / PubMed:40579438 |
| 手法 | EM (単粒子) |
| 解像度 | 2.9 - 3.3 Å |
| 構造データ | EMDB-60605, PDB-9iiu: EMDB-60694, PDB-9imn: EMDB-60748, PDB-9iox: ![]() EMDB-60827: The consensus map of TEF30-associated C2S-type PSII supercomplex ![]() EMDB-60828: The local map of the TEF30-associated PSII core in TEF30-C2S ![]() EMDB-60829: The local map of the CS-PsbO part in TEF30-C2S EMDB-60830, PDB-9is4: |
| 化合物 | ![]() ChemComp-FE2: ![]() ChemComp-CLA: ![]() ChemComp-PHO: ![]() ChemComp-BCR: ![]() ChemComp-SQD: ![]() ChemComp-LMG: ![]() ChemComp-LHG: ![]() ChemComp-DGD: ![]() ChemComp-LMU: ![]() ChemComp-BCT: ![]() ChemComp-PL9: ![]() ChemComp-HEM: ![]() ChemComp-HOH: ![]() ChemComp-CHL: ![]() ChemComp-LUT: ![]() ChemComp-NEX: ![]() ChemComp-XAT: |
| 由来 |
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キーワード | PHOTOSYNTHESIS / photosystem II / PSII repair / PSII assembly / PSII intermediate / TEF30 / C2S / supercomplex |
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