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- EMDB-60022: Drosophila mojavensis gustatory receptor 43a(Gr43a) in Fructose-b... -

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Basic information

Entry
Database: EMDB / ID: EMD-60022
TitleDrosophila mojavensis gustatory receptor 43a(Gr43a) in Fructose-bound state
Map data
Sample
  • Complex: Tetrameric fructose-specific receptor 43a of Drosophila mojavensis (DmGr43a)
    • Protein or peptide: Gustatory receptor
  • Ligand: beta-D-fructofuranose
Keywordsgustatory receptors / ion channel / fructose / PROTEIN TRANSPORT
Function / homology7TM chemoreceptor / 7tm Chemosensory receptor / sensory perception of taste / signal transduction / plasma membrane / Gustatory receptor
Function and homology information
Biological speciesDrosophila mojavensis (fry)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.17 Å
AuthorsChen QF / Chen RZ / Zhang R
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32071202 China
National Natural Science Foundation of China (NSFC)32371261 China
CitationJournal: Cell Discov / Year: 2024
Title: Structure basis for sugar specificity of gustatory receptors in insects.
Authors: Ruizhu Chen / Ran Zhang / Lu Li / Bozhan Wang / Zhiwei Gao / Fenglian Liu / Yan Chen / Yutao Tian / Baobin Li / Qingfeng Chen /
History
DepositionMay 4, 2024-
Header (metadata) releaseOct 30, 2024-
Map releaseOct 30, 2024-
UpdateNov 6, 2024-
Current statusNov 6, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_60022.png

Downloads & links

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Map

FileDownload / File: emd_60022.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 220 pix.
= 205.04 Å		0.93 Å/pix.
x 220 pix.
= 205.04 Å		0.93 Å/pix.
x 220 pix.
= 205.04 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.932 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.35
Minimum - Maximum-2.0635686 - 2.9608736
Average (Standard dev.)0.017273637 (±0.11920953)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions220220220
Spacing220220220
CellA=B=C: 205.04 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_60022_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_60022_half_map_2.map
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Sample components

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Entire : Tetrameric fructose-specific receptor 43a of Drosophila mojavensi...

EntireName: Tetrameric fructose-specific receptor 43a of Drosophila mojavensis (DmGr43a)
Components
  • Complex: Tetrameric fructose-specific receptor 43a of Drosophila mojavensis (DmGr43a)
    • Protein or peptide: Gustatory receptor
  • Ligand: beta-D-fructofuranose

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Supramolecule #1: Tetrameric fructose-specific receptor 43a of Drosophila mojavensi...

SupramoleculeName: Tetrameric fructose-specific receptor 43a of Drosophila mojavensis (DmGr43a)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Drosophila mojavensis (fry)
Molecular weightTheoretical: 47.573 KDa

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Macromolecule #1: Gustatory receptor

MacromoleculeName: Gustatory receptor / type: protein_or_peptide / ID: 1
Details: residues 436-443 correspond to the expression tag ,and residues 428-435 correspond to flexible linker.
Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Drosophila mojavensis (fry)
Molecular weightTheoretical: 50.13257 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MEISETNIGI FYVSKLLALA PYSAKKNSKG QLEITRSWLF SIYSVCLCLI MVFLTYRGLL FDANSNIPVR MKSATSKVVT ASDVSVVVL AIVTGVYCGM FGLRATQELN TRLDKIDSTL SPYNNVKKDR WRAYAMATVS LIVIGILLGL DVGTWVRMAQ E MNISDEDT ...String:
MEISETNIGI FYVSKLLALA PYSAKKNSKG QLEITRSWLF SIYSVCLCLI MVFLTYRGLL FDANSNIPVR MKSATSKVVT ASDVSVVVL AIVTGVYCGM FGLRATQELN TRLDKIDSTL SPYNNVKKDR WRAYAMATVS LIVIGILLGL DVGTWVRMAQ E MNISDEDT ELNVQWYIPF YSLYFILTGL HINFANTAYG LGRRYKRLNQ MLRTSYLSAD KSYGNATNLV KVSTVKSISF KP MMPMALH ASLTKLNTET LPNESATKNK SLLIRAMADN HESLGKCVRL LSRFYGIAVL FILVSCLLHL VATAYFLFLE MLN KRDNGY VWVQMLWIIF HFLRLLMVVE PCHLAAREAR KTIQIVCEIE RKVFEPILVE ETKKFWQQLL VDDAEFSASG LCRV NRTIL TSFASAIATY LVILIQFQKT NGLEGGSSGG WSHPQFEK

UniProtKB: Gustatory receptor

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Macromolecule #2: beta-D-fructofuranose

MacromoleculeName: beta-D-fructofuranose / type: ligand / ID: 2 / Number of copies: 4 / Formula: FRU
Molecular weightTheoretical: 180.156 Da
Chemical component information

ChemComp-FRU:
beta-D-fructofuranose

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration4.00 mg/mL
BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 48.66 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.17 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 82442
Initial angle assignmentType: OTHER
Final angle assignmentType: PROJECTION MATCHING

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Atomic model buiding 1

RefinementProtocol: AB INITIO MODEL
Output model

PDB-8ze3:
Drosophila mojavensis gustatory receptor 43a(Gr43a) in Fructose-bound state

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