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- PDB-8ze3: Drosophila mojavensis gustatory receptor 43a(Gr43a) in Fructose-b... -

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Basic information

Entry
Database: PDB / ID: 8ze3
TitleDrosophila mojavensis gustatory receptor 43a(Gr43a) in Fructose-bound state
ComponentsGustatory receptor
KeywordsPROTEIN TRANSPORT / gustatory receptors / ion channel / fructose
Function / homology
Function and homology information


male courtship behavior / chemosensory behavior / sensory perception of taste / axon / neuronal cell body / dendrite / signal transduction / plasma membrane
Similarity search - Function
7TM chemoreceptor / 7tm Chemosensory receptor
Similarity search - Domain/homology
beta-D-fructofuranose / Gustatory receptor
Similarity search - Component
Biological speciesDrosophila mojavensis (fry)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.17 Å
AuthorsChen, Q.F. / Chen, R.Z. / Zhang, R.
Funding support China, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32071202 China
National Natural Science Foundation of China (NSFC)32371261 China
CitationJournal: Cell Discov / Year: 2024
Title: Structure basis for sugar specificity of gustatory receptors in insects.
Authors: Ruizhu Chen / Ran Zhang / Lu Li / Bozhan Wang / Zhiwei Gao / Fenglian Liu / Yan Chen / Yutao Tian / Baobin Li / Qingfeng Chen /
History
DepositionMay 4, 2024Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Oct 30, 2024Provider: repository / Type: Initial release
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Revision 1.1Jul 23, 2025Data content type: EM metadata / Data content type: EM metadata / EM metadata / Group: Data processing / Experimental summary / Data content type: EM metadata / EM metadata / Category: em_admin / em_software / Data content type: EM metadata / EM metadata / Item: _em_admin.last_update / _em_software.name

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Gustatory receptor
B: Gustatory receptor
C: Gustatory receptor
D: Gustatory receptor
hetero molecules


Theoretical massNumber of molelcules
Total (without water)201,2518
Polymers200,5304
Non-polymers7214
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein
Gustatory receptor


Mass: 50132.570 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Details: residues 436-443 correspond to the expression tag ,and residues 428-435 correspond to flexible linker.
Source: (gene. exp.) Drosophila mojavensis (fry) / Gene: Dmoj\GI20824, Dmoj_GI20824 / Production host: Homo sapiens (human) / References: UniProt: B4KNE2
#2: Sugar
ChemComp-FRU / beta-D-fructofuranose / beta-D-fructose / D-fructose / fructose


Type: D-saccharide, beta linking / Mass: 180.156 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C6H12O6 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DFrufbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
b-D-fructofuranoseCOMMON NAMEGMML 1.0
b-D-FrufIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
FruSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Tetrameric fructose-specific receptor 43a of Drosophila mojavensis (DmGr43a)
Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT
Molecular weightValue: 0.047573 MDa / Experimental value: NO
Source (natural)Organism: Drosophila mojavensis (fry)
Source (recombinant)Organism: Homo sapiens (human) / Cell: HEK293F / Plasmid: PEZT-BM
Buffer solutionpH: 8
SpecimenConc.: 4 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 800 nm
Image recordingElectron dose: 48.66 e/Å2 / Film or detector model: TFS FALCON 4i (4k x 4k)

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Processing

EM softwareName: PHENIX / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.17 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 82442 / Symmetry type: POINT
Atomic model buildingProtocol: AB INITIO MODEL
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00312256
ELECTRON MICROSCOPYf_angle_d0.60216588
ELECTRON MICROSCOPYf_dihedral_angle_d3.8851640
ELECTRON MICROSCOPYf_chiral_restr0.0371968
ELECTRON MICROSCOPYf_plane_restr0.0042008

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