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- EMDB-60021: Drosophila melanogaster gustatory receptor 64a(Gr64a) in Sucrose-... -

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Basic information

Entry
Database: EMDB / ID: EMD-60021
TitleDrosophila melanogaster gustatory receptor 64a(Gr64a) in Sucrose-bound state
Map data
Sample
  • Complex: Tetrameric sugar receptor Gr64a of Drosophila melanogaster (DmGr64a)
    • Protein or peptide: Gustatory receptor for sugar taste 64a
Keywordsgustatory receptors / ion channel / Sucrose / PROTEIN TRANSPORT
Function / homology
Function and homology information


proboscis extension reflex / detection of chemical stimulus involved in sensory perception of taste / ionotropic sweet taste receptor activity / sweet taste receptor activity / taste receptor activity / sensory perception of sweet taste / ligand-gated monoatomic ion channel activity / sensory perception of taste / monoatomic ion transmembrane transport / signal transduction ...proboscis extension reflex / detection of chemical stimulus involved in sensory perception of taste / ionotropic sweet taste receptor activity / sweet taste receptor activity / taste receptor activity / sensory perception of sweet taste / ligand-gated monoatomic ion channel activity / sensory perception of taste / monoatomic ion transmembrane transport / signal transduction / membrane / plasma membrane
Similarity search - Function
Gustatory receptor / Trehalose receptor
Similarity search - Domain/homology
Gustatory receptor for sugar taste 64a
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.57 Å
AuthorsChen QF / Chen RZ / Zhang R
Funding support China, 2 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32071202 China
National Natural Science Foundation of China (NSFC)32371261 China
CitationJournal: Cell Discov / Year: 2024
Title: Structure basis for sugar specificity of gustatory receptors in insects.
Authors: Ruizhu Chen / Ran Zhang / Lu Li / Bozhan Wang / Zhiwei Gao / Fenglian Liu / Yan Chen / Yutao Tian / Baobin Li / Qingfeng Chen /
History
DepositionMay 4, 2024-
Header (metadata) releaseOct 30, 2024-
Map releaseOct 30, 2024-
UpdateNov 6, 2024-
Current statusNov 6, 2024Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_60021.png

Downloads & links

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Map

FileDownload / File: emd_60021.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 220 pix.
= 205.04 Å		0.93 Å/pix.
x 220 pix.
= 205.04 Å		0.93 Å/pix.
x 220 pix.
= 205.04 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.932 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.118
Minimum - Maximum-1.544396 - 2.3898342
Average (Standard dev.)0.005236252 (±0.08270966)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions220220220
Spacing220220220
CellA=B=C: 205.04 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_60021_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_60021_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Tetrameric sugar receptor Gr64a of Drosophila melanogaster (DmGr64a)

EntireName: Tetrameric sugar receptor Gr64a of Drosophila melanogaster (DmGr64a)
Components
  • Complex: Tetrameric sugar receptor Gr64a of Drosophila melanogaster (DmGr64a)
    • Protein or peptide: Gustatory receptor for sugar taste 64a

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Supramolecule #1: Tetrameric sugar receptor Gr64a of Drosophila melanogaster (DmGr64a)

SupramoleculeName: Tetrameric sugar receptor Gr64a of Drosophila melanogaster (DmGr64a)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Molecular weightTheoretical: 52.823 KDa

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Macromolecule #1: Gustatory receptor for sugar taste 64a

MacromoleculeName: Gustatory receptor for sugar taste 64a / type: protein_or_peptide / ID: 1
Details: residues 465-472 correspond to the expression tag ,and residues 457-464 correspond to flexible linker.
Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Molecular weightTheoretical: 54.565121 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MKGPNLNFRK TPSKDNGVKQ VESLARPETP PPKFVEDSNL EFNVLASEKL PNYTNLDLFH RAVFPFMFLA QCVAIMPLVG IRESNPRRV RFAYKSIPMF VTLIFMIATS ILFLSMFTHL LKIGITAKNF VGLVFFGCVL SAYVVFIRLA KKWPAVVRIW T RTEIPFTK ...String:
MKGPNLNFRK TPSKDNGVKQ VESLARPETP PPKFVEDSNL EFNVLASEKL PNYTNLDLFH RAVFPFMFLA QCVAIMPLVG IRESNPRRV RFAYKSIPMF VTLIFMIATS ILFLSMFTHL LKIGITAKNF VGLVFFGCVL SAYVVFIRLA KKWPAVVRIW T RTEIPFTK PPYEIPKRNL SRRVQLAALA IIGLSLGEHA LYQVSAILSY TRRIQMCANI TTVPSFNNYM QTNYDYVFQL LP YSPIIAV LILLINGACT FVWNYMDLFI MMISKGLSYR FEQITTRIRK LEHEEVCESV FIQIREHYVK MCELLEFVDS AMS SLILLS CVNNLYFVCY QLLNVFNKLR WPINYIYFWY SLLYLIGRTA FVFLTAADIN EESKRGLGVL RRVSSRSWCV EVER LIFQM TTQTVALSGK KFYFLTRRLL FGMAGTIVTY ELVLLQFDEP NRRKGLQPLC ALEGGSSGGW SHPQFEK

UniProtKB: Gustatory receptor for sugar taste 64a

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration4.00 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Average electron dose: 48.24 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.57 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 413565
Initial angle assignmentType: OTHER
Final angle assignmentType: PROJECTION MATCHING

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Atomic model buiding 1

RefinementProtocol: AB INITIO MODEL
Output model

PDB-8ze2:
Drosophila melanogaster gustatory receptor 64a(Gr64a) in Sucrose-bound state

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