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データを開く
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基本情報
登録情報 | ![]() | ||||||||||||||||||
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タイトル | Mammalian quaternary complex of a translating 80S ribosome, NAC, MetAP1 and NatA/E | ||||||||||||||||||
![]() | homogeneously refined cryo-EM map of the quaternary RNC-NAC-NatA/E-MetAP1 complex | ||||||||||||||||||
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![]() | translation / ribosome / NAC / N-terminal acetyltransferase / NatA / NatE / MetAP1 | ||||||||||||||||||
機能・相同性 | ![]() negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / regulation of skeletal muscle fiber development / : / negative regulation of striated muscle cell apoptotic process / mitotic sister chromatid cohesion, centromeric / peptide-glutamate-alpha-N-acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / N-terminal amino-acid Nalpha-acetyltransferase NatA ...negative regulation of maintenance of mitotic sister chromatid cohesion, centromeric / negative regulation of protein localization to endoplasmic reticulum / nascent polypeptide-associated complex / regulation of skeletal muscle fiber development / : / negative regulation of striated muscle cell apoptotic process / mitotic sister chromatid cohesion, centromeric / peptide-glutamate-alpha-N-acetyltransferase activity / N-terminal methionine Nalpha-acetyltransferase NatE / N-terminal amino-acid Nalpha-acetyltransferase NatA / positive regulation of cell proliferation involved in heart morphogenesis / peptide-serine-alpha-N-acetyltransferase activity / NatA complex / N-terminal protein amino acid acetylation / positive regulation of skeletal muscle tissue growth / cardiac ventricle development / peptide alpha-N-acetyltransferase activity / N-terminal protein amino acid modification / peptidyl-methionine modification / skeletal muscle tissue regeneration / initiator methionyl aminopeptidase activity / histone H4 acetyltransferase activity / heart trabecula morphogenesis / establishment of mitotic sister chromatid cohesion / methionyl aminopeptidase / negative regulation of formation of translation preinitiation complex / metalloexopeptidase activity / N-acetyltransferase activity / GAIT complex / regulation of G1 to G0 transition / laminin receptor activity / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / mitotic sister chromatid cohesion / optic nerve development / retinal ganglion cell axon guidance / mammalian oogenesis stage / G1 to G0 transition / homeostatic process / internal protein amino acid acetylation / protein acetylation / activation-induced cell death of T cells / macrophage chemotaxis / lung morphogenesis / metalloaminopeptidase activity / positive regulation of signal transduction by p53 class mediator / glutathione transferase / phagocytic cup / ubiquitin ligase inhibitor activity / protein maturation / glutathione transferase activity / TOR signaling / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / chromosome organization / 90S preribosome / T cell proliferation involved in immune response / protein-RNA complex assembly / erythrocyte development / negative regulation of ubiquitin-dependent protein catabolic process / translation regulator activity / cellular response to actinomycin D / ribosomal small subunit export from nucleus / aminopeptidase activity / cytosolic ribosome / laminin binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rough endoplasmic reticulum / gastrulation / MDM2/MDM4 family protein binding / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / glutathione metabolic process / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / rescue of stalled ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / cellular response to leukemia inhibitory factor / maturation of SSU-rRNA / placenta development / small-subunit processome / positive regulation of translation / protein kinase C binding / wound healing / positive regulation of protein-containing complex assembly / G1/S transition of mitotic cell cycle / cellular response to gamma radiation / cellular response to type II interferon / platelet aggregation / transcription coactivator binding / mRNA 5'-UTR binding / modification-dependent protein catabolic process / spindle / cytoplasmic ribonucleoprotein granule / positive regulation of canonical Wnt signaling pathway 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() ![]() | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.78 Å | ||||||||||||||||||
![]() | Yudin D / Scaiola A / Ban N | ||||||||||||||||||
資金援助 | ![]() ![]() ![]()
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![]() | ![]() タイトル: NAC guides a ribosomal multienzyme complex for nascent protein processing 著者: Lentzsch AM / Yudin D / Gamerdinger M / Chandrasekar S / Rabl L / Scaiola A / Deuerling E / Ban N / Shan S | ||||||||||||||||||
履歴 |
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構造の表示
添付画像 |
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 336.2 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 136.7 KB 136.7 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 21 KB | 表示 | ![]() |
画像 | ![]() | 138.9 KB | ||
マスクデータ | ![]() ![]() | 669.9 MB 669.9 MB | ![]() | |
Filedesc metadata | ![]() | 25.1 KB | ||
その他 | ![]() ![]() ![]() ![]() ![]() | 626.8 MB 625.5 MB 34 MB 621.6 MB 621.6 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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注釈 | homogeneously refined cryo-EM map of the quaternary RNC-NAC-NatA/E-MetAP1 complex | ||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.065 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-マスク #1
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投影像・断面図 |
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密度ヒストグラム |
-マスク #2
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投影像・断面図 |
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密度ヒストグラム |
-追加マップ: main map lowpass filtered to 6 A resolution
ファイル | emd_50125_additional_1.map | ||||||||||||
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注釈 | main map lowpass filtered to 6 A resolution | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: main map lowpass filtered to 8 A resolution
ファイル | emd_50125_additional_2.map | ||||||||||||
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注釈 | main map lowpass filtered to 8 A resolution | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-追加マップ: main map lowpass filtered to estimated local resolution
ファイル | emd_50125_additional_3.map | ||||||||||||
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注釈 | main map lowpass filtered to estimated local resolution | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: halfmap A
ファイル | emd_50125_half_map_1.map | ||||||||||||
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注釈 | halfmap A | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: halfmap B
ファイル | emd_50125_half_map_2.map | ||||||||||||
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注釈 | halfmap B | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Quaternary complex of a translating 80S ribosome, NAC, MetAP1 and...
+超分子 #1: Quaternary complex of a translating 80S ribosome, NAC, MetAP1 and...
+超分子 #2: Rabbit ribosome-nascent chain complex
+超分子 #3: NAC heterodimer
+超分子 #4: Human methionine aminopeptidase 1
+超分子 #5: Human NatA/E complex
+超分子 #6: Human NatA complex
+超分子 #7: Human NatE with a GST tag
+分子 #1: mRNA
+分子 #3: 5S rRNA
+分子 #4: P-site tRNA
+分子 #6: 5.8S rRNA
+分子 #20: 28S rRNA
+分子 #64: 18S rRNA
+分子 #2: 60S ribosomal protein L29
+分子 #5: 40S ribosomal protein S18
+分子 #7: 60S ribosomal protein L22
+分子 #8: 40S ribosomal protein S19
+分子 #9: Large ribosomal subunit protein uL2
+分子 #10: Ribosomal protein L23
+分子 #11: Small ribosomal subunit protein uS10
+分子 #12: Ribosomal protein L3
+分子 #13: Large ribosomal subunit protein uL23
+分子 #14: Small ribosomal subunit protein eS21
+分子 #15: 60S ribosomal protein L4
+分子 #16: Large ribosomal subunit protein uL22
+分子 #17: 60S ribosomal protein L27
+分子 #18: 40S ribosomal protein S23
+分子 #19: 60S ribosomal protein L6
+分子 #21: Large ribosomal subunit protein eL18
+分子 #22: 60S ribosomal protein L27a
+分子 #23: 40S ribosomal protein S24
+分子 #24: Ribosomal Protein uL30
+分子 #25: Ribosomal protein L26
+分子 #26: Ribosomal protein L24
+分子 #27: 40S ribosomal protein SA
+分子 #28: 40S ribosomal protein S25
+分子 #29: 60S ribosomal protein L7a
+分子 #30: Ribosomal protein S15a
+分子 #31: Ribosomal protein L19
+分子 #32: 40S ribosomal protein S3a
+分子 #33: 60S ribosomal protein L41
+分子 #34: 60S ribosomal protein L9
+分子 #35: Large ribosomal subunit protein eL20
+分子 #36: Small ribosomal subunit protein uS5
+分子 #37: 60S ribosomal protein L30
+分子 #38: 60S ribosomal protein L10
+分子 #39: Large ribosomal subunit protein uL18
+分子 #40: Methionine aminopeptidase 1
+分子 #41: 40S ribosomal protein S3
+分子 #42: 60S ribosomal protein L31
+分子 #43: 60S ribosomal protein L11
+分子 #44: Nascent polypeptide-associated complex subunit alpha
+分子 #45: 40S ribosomal protein S4
+分子 #46: Ribosomal protein L32
+分子 #47: Nascent chain
+分子 #48: Transcription factor BTF3
+分子 #49: Ribosomal protein S5
+分子 #50: 60S ribosomal protein L35a
+分子 #51: Large ribosomal subunit protein eL13
+分子 #52: Glutathione S-transferase class-mu 26 kDa isozyme,N-alpha-acetylt...
+分子 #53: 40S ribosomal protein S6
+分子 #54: 60S ribosomal protein L34
+分子 #55: 60S ribosomal protein L14
+分子 #56: N-alpha-acetyltransferase 15, NatA auxiliary subunit
+分子 #57: 40S ribosomal protein S7
+分子 #58: 60S ribosomal protein L35
+分子 #59: Ribosomal protein L15
+分子 #60: N-alpha-acetyltransferase 10
+分子 #61: 40S ribosomal protein S8
+分子 #62: 60S ribosomal protein L36
+分子 #63: Large ribosomal subunit protein uL13
+分子 #65: 40S ribosomal protein S9
+分子 #66: Ribosomal protein L37
+分子 #67: 40S ribosomal protein S27
+分子 #68: S10_plectin domain-containing protein
+分子 #69: 60S ribosomal protein L38
+分子 #70: 40S ribosomal protein S28
+分子 #71: 40S ribosomal protein S11
+分子 #72: 60S ribosomal protein L39-like
+分子 #73: Ribosomal protein S27a
+分子 #74: 40S ribosomal protein S12
+分子 #75: Large ribosomal subunit protein eL40
+分子 #76: 40S ribosomal protein S30
+分子 #77: 40S ribosomal protein S13
+分子 #78: Large ribosomal subunit protein eL42
+分子 #79: Small ribosomal subunit protein eS26
+分子 #80: Small ribosomal subunit protein uS11
+分子 #81: 60S ribosomal protein L37a
+分子 #82: Small ribosomal subunit protein RACK1
+分子 #83: 40S ribosomal protein uS19
+分子 #84: 60S ribosomal protein L28
+分子 #85: 40S ribosomal protein S29
+分子 #86: Small ribosomal subunit protein uS9
+分子 #87: Large ribosomal subunit protein uL10
+分子 #88: 60S ribosomal protein L21
+分子 #89: 40S ribosomal protein eS17
+分子 #90: 60S ribosomal protein L12
+分子 #91: UNKNOWN ATOM OR ION
+分子 #92: MAGNESIUM ION
+分子 #93: GUANOSINE-5'-TRIPHOSPHATE
+分子 #94: SPERMIDINE
+分子 #95: SPERMINE
+分子 #96: ZINC ION
+分子 #97: INOSITOL HEXAKISPHOSPHATE
+分子 #98: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE-PROPANE |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: OTHER / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: SPOT SCAN / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.4 µm / 最小 デフォーカス(公称値): 0.6 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |