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- EMDB-48183: Antibody fragments from mAb475 and mAb824 bound to the adhesin pr... -

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Entry
Database: EMDB / ID: EMD-48183
TitleAntibody fragments from mAb475 and mAb824 bound to the adhesin protein FimH
Map dataAntibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
Sample
  • Complex: Antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
    • Complex: Fimbrial tip
      • Protein or peptide: Type 1 fimbrin D-mannose specific adhesin
      • Protein or peptide: Protein FimG
    • Complex: Antibody Fragments
      • Protein or peptide: mAb475 Heavy Chain Fragment
      • Protein or peptide: mAb824 Heavy Chain Fragment
      • Protein or peptide: mAb824 Light Chain Fragment
  • Protein or peptide: mAb475 Light Chain Fragment
KeywordsFimbrial tip / Lectin domain / Antibody fragments / Antibody-target complex / CELL ADHESION / CELL ADHESION-IMMUNE SYSTEM complex
Function / homology
Function and homology information


pilus tip / mechanosensory behavior / cell adhesion involved in single-species biofilm formation / pilus / cell-substrate adhesion / D-mannose binding / host cell membrane / cell adhesion
Similarity search - Function
FimH, mannose-binding domain / FimH, mannose binding / Fimbrial-type adhesion domain / Fimbrial protein / : / Fimbrial-type adhesion domain superfamily / Adhesion domain superfamily
Similarity search - Domain/homology
Protein FimG / Type 1 fimbrin D-mannose specific adhesin
Similarity search - Component
Biological speciesEscherichia coli (E. coli) / Mus musculus (house mouse) / Escherichia coli K-12 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsHvorecny KL / Magala P / Klevit RE / Kollman JM
Funding support United States, 4 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35 GM149542 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)F32 AI145111 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI171570 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)K99 GM141364 United States
CitationJournal: Nat Commun / Year: 2025
Title: Antibodies disrupt bacterial adhesion by ligand mimicry and allosteric interference.
Authors: Kelli L Hvorecny / Gianluca Interlandi / Tim S Veth / Pavel Aprikian / Anna Manchenko / Veronika L Tchesnokova / Miles S Dickinson / Joel D Quispe / Nicholas M Riley / Rachel E Klevit / ...Authors: Kelli L Hvorecny / Gianluca Interlandi / Tim S Veth / Pavel Aprikian / Anna Manchenko / Veronika L Tchesnokova / Miles S Dickinson / Joel D Quispe / Nicholas M Riley / Rachel E Klevit / Pearl Magala / Evgeni V Sokurenko / Justin M Kollman /
Abstract: A critical step in infections is the attachment of microorganisms to host cells using lectins that bind glycans, making lectins promising antimicrobial targets. Upon binding mannosylated glycans, ...A critical step in infections is the attachment of microorganisms to host cells using lectins that bind glycans, making lectins promising antimicrobial targets. Upon binding mannosylated glycans, FimH, an adhesin in E. coli, undergoes an allosteric transition from an inactive to an active conformation that can act as a catch-bond. Distinct monoclonal antibodies that alter FimH glycan binding are available, but the mechanisms of action remain unclear. Here, we use cryo-electron microscopy, mass spectrometry, adhesion assays, and molecular dynamics simulations to determine the structure-function relationships underlying antibody-FimH binding. Our study demonstrates four mechanisms of action: ligand mimicry by an N-linked, high-mannose glycan; stabilization of the ligand pocket in the inactive state; conformational trapping of the active and inactive states; and locking of the ligand pocket through long-range allosteric effects. These structures reveal multiple mechanisms of antibody responses to an allosteric protein and provide blueprints for antimicrobials that target adhesins.
History
DepositionDec 6, 2024-
Header (metadata) releaseNov 26, 2025-
Map releaseNov 26, 2025-
UpdateDec 10, 2025-
Current statusDec 10, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48183.png

Downloads & links

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Map

FileDownload / File: emd_48183.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationAntibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
1.12 Å/pix.
x 480 pix.
= 539.52 Å		1.12 Å/pix.
x 480 pix.
= 539.52 Å		1.12 Å/pix.
x 480 pix.
= 539.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.124 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 4.5
Minimum - Maximum-21.593575999999999 - 32.187800000000003
Average (Standard dev.)0.000001074025 (±0.1800143)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 539.51996 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Antibody fragments from mAb475 and mAb824 bound to...

Fileemd_48183_additional_1.map
AnnotationAntibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH masked around variable regions and FimH lectin domain
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half map of antibody fragments from mAb475 and...

Fileemd_48183_additional_2.map
AnnotationHalf map of antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH masked around variable regions and FimH lectin domain
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half map of antibody fragments from mAb475 and...

Fileemd_48183_additional_3.map
AnnotationHalf map of antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH masked around variable regions and FimH lectin domain
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of antibody fragments from mAb475 and...

Fileemd_48183_half_map_1.map
AnnotationHalf map of antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of antibody fragments from mAb475 and...

Fileemd_48183_half_map_2.map
AnnotationHalf map of antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Antibody fragments from mAb475 and mAb824 bound to the E. coli ad...

EntireName: Antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
Components
  • Complex: Antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
    • Complex: Fimbrial tip
      • Protein or peptide: Type 1 fimbrin D-mannose specific adhesin
      • Protein or peptide: Protein FimG
    • Complex: Antibody Fragments
      • Protein or peptide: mAb475 Heavy Chain Fragment
      • Protein or peptide: mAb824 Heavy Chain Fragment
      • Protein or peptide: mAb824 Light Chain Fragment
  • Protein or peptide: mAb475 Light Chain Fragment

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Supramolecule #1: Antibody fragments from mAb475 and mAb824 bound to the E. coli ad...

SupramoleculeName: Antibody fragments from mAb475 and mAb824 bound to the E. coli adhesin protein FimH
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4, #6

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Supramolecule #2: Fimbrial tip

SupramoleculeName: Fimbrial tip / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#2
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #3: Antibody Fragments

SupramoleculeName: Antibody Fragments / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #3-#4, #6
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma

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Macromolecule #1: Type 1 fimbrin D-mannose specific adhesin

MacromoleculeName: Type 1 fimbrin D-mannose specific adhesin / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 31.48826 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKRVITLFAV LLMGWSVNAW SFACKTANGT AIPIGGGSAN VYVNLAPVVN VGQNLVVDLS TQIFCHNDYP ETITDYVTLQ RGSAYGGVL SNFSGTVKYS GSSYPFPTTS ETPRVVYNSR TDKPWPVALY LTPVSSAGGV AIKAGSLIAV LILRQTNNYN S DDFQFVWN ...String:
MKRVITLFAV LLMGWSVNAW SFACKTANGT AIPIGGGSAN VYVNLAPVVN VGQNLVVDLS TQIFCHNDYP ETITDYVTLQ RGSAYGGVL SNFSGTVKYS GSSYPFPTTS ETPRVVYNSR TDKPWPVALY LTPVSSAGGV AIKAGSLIAV LILRQTNNYN S DDFQFVWN IYANNDVVVP TGGCDVSARD VTVTLPDYPG SVPIPLTVYC AKSQNLGYYL SGTTADAGNS IFTNTASFSP AQ GVGVQLT RNGTIIPANN TVSLGAVGTS AVSLGLTANY ARTGGQVTAG NVQSIIGVTF VYQ

UniProtKB: Type 1 fimbrin D-mannose specific adhesin

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Macromolecule #2: Protein FimG

MacromoleculeName: Protein FimG / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli K-12 (bacteria) / Strain: K-12
Molecular weightTheoretical: 17.328258 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MKWCKRGYVL AAILALASAT IQAADVTITV NGKVVAKPCT VSTTNATVDL GDLYSFSLMS AGAASAWHDV ALELTNCPVG TSRVTASFS GAADSTGYYK NQGTAQNIQL ELQDDSGNTL NTGATKTVQV DDSSQSAHFP LQVRALTVNG GATQGTIQAV I SITYTYS

UniProtKB: Protein FimG

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Macromolecule #3: mAb475 Heavy Chain Fragment

MacromoleculeName: mAb475 Heavy Chain Fragment / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 23.105812 KDa
SequenceString: QVQLQQSGAE LVRPGSSVKI SCKASGYAFS SYWMNWVKQR PGQGLEWIGQ IYPRDGDTNY NGKFMDKVTL TADKSSNTAY MQLSSLTSE DSAVYFCEVG RGFYGMDYWG QGTSVTVSSA KTTPPSVYPL APGSAAQTNS MVTLGCLVKG YFPEPVTVTW N SGSLSSGV ...String:
QVQLQQSGAE LVRPGSSVKI SCKASGYAFS SYWMNWVKQR PGQGLEWIGQ IYPRDGDTNY NGKFMDKVTL TADKSSNTAY MQLSSLTSE DSAVYFCEVG RGFYGMDYWG QGTSVTVSSA KTTPPSVYPL APGSAAQTNS MVTLGCLVKG YFPEPVTVTW N SGSLSSGV HTFPAVLQSD LYTLSSSVTV PSSPRPSETV TCNVAHPASS TKVDKKI

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Macromolecule #4: mAb824 Heavy Chain Fragment

MacromoleculeName: mAb824 Heavy Chain Fragment / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 22.526971 KDa
SequenceString: EIQLQQSGPE RMKPGASVKI SCKASGYSFT TYYIHWVKQS HGRSLEWIGY IDPFNDDTNY NQKFKGKATL TVDKSSSTAY MHLSSLTSE DSAVYYCARS YYGSLDYWGQ GTTLTVSSAK TTPPSVYPLA PGSAAQTNSM VTLGCLVKGY FPEPVTVTWN S GSLSSGVH ...String:
EIQLQQSGPE RMKPGASVKI SCKASGYSFT TYYIHWVKQS HGRSLEWIGY IDPFNDDTNY NQKFKGKATL TVDKSSSTAY MHLSSLTSE DSAVYYCARS YYGSLDYWGQ GTTLTVSSAK TTPPSVYPLA PGSAAQTNSM VTLGCLVKGY FPEPVTVTWN S GSLSSGVH TFPAVLQSDL YTLSSSVTVP SSTWPSETVT CNVAHPASST

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Macromolecule #5: mAb475 Light Chain Fragment

MacromoleculeName: mAb475 Light Chain Fragment / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 23.496877 KDa
SequenceString: ELQMTQSPKF MSTSVGDRVS VTCKASQNVS NVAWYQQKPG QSPKAMIYSA SYRYSGVPGR FTGSGSGTDF TLTINNVQSE DLATYFCQQ NSSFPFTFGG GTKLEIKRAD AAPTVSIFPP SSEQLTSGGA SVVCFLNNFY PKDINVKWKI DGSERQNGVL N SWTDQDSK ...String:
ELQMTQSPKF MSTSVGDRVS VTCKASQNVS NVAWYQQKPG QSPKAMIYSA SYRYSGVPGR FTGSGSGTDF TLTINNVQSE DLATYFCQQ NSSFPFTFGG GTKLEIKRAD AAPTVSIFPP SSEQLTSGGA SVVCFLNNFY PKDINVKWKI DGSERQNGVL N SWTDQDSK DSTYSMSSTL TLTKDEYERH NSYTCEATHK TSTSPIVKSF NRNEC

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Macromolecule #6: mAb824 Light Chain Fragment

MacromoleculeName: mAb824 Light Chain Fragment / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 21.757852 KDa
SequenceString: DIQMTQTTSS LSASLGDRVT ISCRASQGVN NYLNWYQQKP DGSVKLLIYY TSNLHSGAPS RFSGSGSGTD YSLTISNLEQ EDIATYFCQ QANMVPWTFG GGTKLEIKRA DAAPTVSIFP PSSEQLTSGG ASVVCFLNNF YPKDINVKWK IDGSERQNGV L NSWTDQDS ...String:
DIQMTQTTSS LSASLGDRVT ISCRASQGVN NYLNWYQQKP DGSVKLLIYY TSNLHSGAPS RFSGSGSGTD YSLTISNLEQ EDIATYFCQ QANMVPWTFG GGTKLEIKRA DAAPTVSIFP PSSEQLTSGG ASVVCFLNNF YPKDINVKWK IDGSERQNGV L NSWTDQDS KDSTYSMSST LTLTKDEYER HNSYTCEAR

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: C-flat-2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 7832 / Average electron dose: 42.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.75 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. v4.4.1) / Number images used: 129751
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 5

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Atomic model buiding 1

Initial model
PDB IDChain

3jwn

source_name: PDB, initial_model_type: experimental model

15c8

source_name: PDB, initial_model_type: experimental model
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-9me4:
Antibody fragments from mAb475 and mAb824 bound to the adhesin protein FimH

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