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- EMDB-48186: Antibody fragments from mAb21 and mAb824 bound to the adhesin pro... -

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Entry
Database: EMDB / ID: EMD-48186
TitleAntibody fragments from mAb21 and mAb824 bound to the adhesin protein FimH containing alpha-methyl mannose
Map dataAntibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
Sample
  • Complex: Antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
    • Complex: Fimbrial tip
      • Protein or peptide: Type 1 fimbrin D-mannose specific adhesin
    • Complex: Antibody Fragments
      • Protein or peptide: mAb824 Heavy Chain Fragment
      • Protein or peptide: mAb21 Heavy Chain Fragment
      • Protein or peptide: mAb824 Light Chain Fragment
      • Protein or peptide: mAb21 Light Chain Fragment
  • Ligand: methyl alpha-D-mannopyranoside
KeywordsFimbrial tip / Lectin domain / Antibody fragments / Antibody-target complex / CELL ADHESION / CELL ADHESION-IMMUNE SYSTEM complex
Function / homology
Function and homology information


pilus tip / mechanosensory behavior / cell adhesion involved in single-species biofilm formation / pilus / cell-substrate adhesion / D-mannose binding / host cell membrane / cell adhesion
Similarity search - Function
FimH, mannose-binding domain / FimH, mannose binding / Fimbrial-type adhesion domain / Fimbrial protein / : / Fimbrial-type adhesion domain superfamily / Adhesion domain superfamily
Similarity search - Domain/homology
Type 1 fimbrin D-mannose specific adhesin
Similarity search - Component
Biological speciesEscherichia coli (E. coli) / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsHvorecny KL / Magala P / Klevit RE / Kollman JM
Funding support United States, 4 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35 GM149542 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)F32 AI145111 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)1R01 AI171570 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)K99 GM141364 United States
CitationJournal: Nat Commun / Year: 2025
Title: Antibodies disrupt bacterial adhesion by ligand mimicry and allosteric interference.
Authors: Kelli L Hvorecny / Gianluca Interlandi / Tim S Veth / Pavel Aprikian / Anna Manchenko / Veronika L Tchesnokova / Miles S Dickinson / Joel D Quispe / Nicholas M Riley / Rachel E Klevit / ...Authors: Kelli L Hvorecny / Gianluca Interlandi / Tim S Veth / Pavel Aprikian / Anna Manchenko / Veronika L Tchesnokova / Miles S Dickinson / Joel D Quispe / Nicholas M Riley / Rachel E Klevit / Pearl Magala / Evgeni V Sokurenko / Justin M Kollman /
Abstract: A critical step in infections is the attachment of microorganisms to host cells using lectins that bind glycans, making lectins promising antimicrobial targets. Upon binding mannosylated glycans, ...A critical step in infections is the attachment of microorganisms to host cells using lectins that bind glycans, making lectins promising antimicrobial targets. Upon binding mannosylated glycans, FimH, an adhesin in E. coli, undergoes an allosteric transition from an inactive to an active conformation that can act as a catch-bond. Distinct monoclonal antibodies that alter FimH glycan binding are available, but the mechanisms of action remain unclear. Here, we use cryo-electron microscopy, mass spectrometry, adhesion assays, and molecular dynamics simulations to determine the structure-function relationships underlying antibody-FimH binding. Our study demonstrates four mechanisms of action: ligand mimicry by an N-linked, high-mannose glycan; stabilization of the ligand pocket in the inactive state; conformational trapping of the active and inactive states; and locking of the ligand pocket through long-range allosteric effects. These structures reveal multiple mechanisms of antibody responses to an allosteric protein and provide blueprints for antimicrobials that target adhesins.
History
DepositionDec 6, 2024-
Header (metadata) releaseNov 26, 2025-
Map releaseNov 26, 2025-
UpdateDec 10, 2025-
Current statusDec 10, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_48186.png

Downloads & links

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Map

FileDownload / File: emd_48186.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationAntibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.12 Å/pix.
x 480 pix.
= 539.52 Å		1.12 Å/pix.
x 480 pix.
= 539.52 Å		1.12 Å/pix.
x 480 pix.
= 539.52 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.124 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 4.5
Minimum - Maximum-22.797409999999999 - 29.597303
Average (Standard dev.)0.00000062915825 (±0.17567925)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 539.51996 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Antibody fragments from mAb21 and mAb824 bound to...

Fileemd_48186_additional_1.map
AnnotationAntibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose, masked around variable regions and FimH lectin domain
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half maps of antibody fragments from mAb21 and...

Fileemd_48186_additional_2.map
AnnotationHalf maps of antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose, masked around variable regions and FimH lectin domain
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half maps of antibody fragments from mAb21 and...

Fileemd_48186_additional_3.map
AnnotationHalf maps of antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose, masked around variable regions and FimH lectin domain
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of antibody fragments from mAb21 and...

Fileemd_48186_half_map_1.map
AnnotationHalf map of antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of antibody fragments from mAb21 and...

Fileemd_48186_half_map_2.map
AnnotationHalf map of antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Antibody fragments from mAb21 and mAb824 bound to the E. coli adh...

EntireName: Antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
Components
  • Complex: Antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
    • Complex: Fimbrial tip
      • Protein or peptide: Type 1 fimbrin D-mannose specific adhesin
    • Complex: Antibody Fragments
      • Protein or peptide: mAb824 Heavy Chain Fragment
      • Protein or peptide: mAb21 Heavy Chain Fragment
      • Protein or peptide: mAb824 Light Chain Fragment
      • Protein or peptide: mAb21 Light Chain Fragment
  • Ligand: methyl alpha-D-mannopyranoside

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Supramolecule #1: Antibody fragments from mAb21 and mAb824 bound to the E. coli adh...

SupramoleculeName: Antibody fragments from mAb21 and mAb824 bound to the E. coli adhesin protein FimH containing alpha-methyl mannose
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5

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Supramolecule #2: Fimbrial tip

SupramoleculeName: Fimbrial tip / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #3: Antibody Fragments

SupramoleculeName: Antibody Fragments / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#5
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma

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Macromolecule #1: Type 1 fimbrin D-mannose specific adhesin

MacromoleculeName: Type 1 fimbrin D-mannose specific adhesin / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 31.48826 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKRVITLFAV LLMGWSVNAW SFACKTANGT AIPIGGGSAN VYVNLAPVVN VGQNLVVDLS TQIFCHNDYP ETITDYVTLQ RGSAYGGVL SNFSGTVKYS GSSYPFPTTS ETPRVVYNSR TDKPWPVALY LTPVSSAGGV AIKAGSLIAV LILRQTNNYN S DDFQFVWN ...String:
MKRVITLFAV LLMGWSVNAW SFACKTANGT AIPIGGGSAN VYVNLAPVVN VGQNLVVDLS TQIFCHNDYP ETITDYVTLQ RGSAYGGVL SNFSGTVKYS GSSYPFPTTS ETPRVVYNSR TDKPWPVALY LTPVSSAGGV AIKAGSLIAV LILRQTNNYN S DDFQFVWN IYANNDVVVP TGGCDVSARD VTVTLPDYPG SVPIPLTVYC AKSQNLGYYL SGTTADAGNS IFTNTASFSP AQ GVGVQLT RNGTIIPANN TVSLGAVGTS AVSLGLTANY ARTGGQVTAG NVQSIIGVTF VYQ

UniProtKB: Type 1 fimbrin D-mannose specific adhesin

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Macromolecule #2: mAb824 Heavy Chain Fragment

MacromoleculeName: mAb824 Heavy Chain Fragment / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 22.526971 KDa
SequenceString: EIQLQQSGPE RMKPGASVKI SCKASGYSFT TYYIHWVKQS HGRSLEWIGY IDPFNDDTNY NQKFKGKATL TVDKSSSTAY MHLSSLTSE DSAVYYCARS YYGSLDYWGQ GTTLTVSSAK TTPPSVYPLA PGSAAQTNSM VTLGCLVKGY FPEPVTVTWN S GSLSSGVH ...String:
EIQLQQSGPE RMKPGASVKI SCKASGYSFT TYYIHWVKQS HGRSLEWIGY IDPFNDDTNY NQKFKGKATL TVDKSSSTAY MHLSSLTSE DSAVYYCARS YYGSLDYWGQ GTTLTVSSAK TTPPSVYPLA PGSAAQTNSM VTLGCLVKGY FPEPVTVTWN S GSLSSGVH TFPAVLQSDL YTLSSSVTVP SSTWPSETVT CNVAHPASST

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Macromolecule #3: mAb21 Heavy Chain Fragment

MacromoleculeName: mAb21 Heavy Chain Fragment / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 21.143547 KDa
SequenceString: EVLLKQSGPE KVKPGASVKI PCKASGYTFT DYNIDWVKQS HGTSLEWIGH LDPNSGGTVY NQKFRGKATL TVDKSSSTAY LELRSLTSE DTAVYYCARS TMGVYRSDGY YAMDYWGQGT SVTVSSAKTT PPSVYPLAPG CGDTTGSSVT LGCLVKGYFP E SVTVTWNS ...String:
EVLLKQSGPE KVKPGASVKI PCKASGYTFT DYNIDWVKQS HGTSLEWIGH LDPNSGGTVY NQKFRGKATL TVDKSSSTAY LELRSLTSE DTAVYYCARS TMGVYRSDGY YAMDYWGQGT SVTVSSAKTT PPSVYPLAPG CGDTTGSSVT LGCLVKGYFP E SVTVTWNS GSLSSSVHTF PALLQSGLYT MSSSVTVPSS

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Macromolecule #4: mAb824 Light Chain Fragment

MacromoleculeName: mAb824 Light Chain Fragment / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 21.757852 KDa
SequenceString: DIQMTQTTSS LSASLGDRVT ISCRASQGVN NYLNWYQQKP DGSVKLLIYY TSNLHSGAPS RFSGSGSGTD YSLTISNLEQ EDIATYFCQ QANMVPWTFG GGTKLEIKRA DAAPTVSIFP PSSEQLTSGG ASVVCFLNNF YPKDINVKWK IDGSERQNGV L NSWTDQDS ...String:
DIQMTQTTSS LSASLGDRVT ISCRASQGVN NYLNWYQQKP DGSVKLLIYY TSNLHSGAPS RFSGSGSGTD YSLTISNLEQ EDIATYFCQ QANMVPWTFG GGTKLEIKRA DAAPTVSIFP PSSEQLTSGG ASVVCFLNNF YPKDINVKWK IDGSERQNGV L NSWTDQDS KDSTYSMSST LTLTKDEYER HNSYTCEAR

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Macromolecule #5: mAb21 Light Chain Fragment

MacromoleculeName: mAb21 Light Chain Fragment / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mus musculus (house mouse) / Tissue: B cell hybridoma
Molecular weightTheoretical: 19.737736 KDa
SequenceString:
QIVLTQSPAI MSASLGEEIT LTCSASSSIS YMHWYQQKSG TSPKILIYST SNQASGVPSR FSGSGSGTFY SLTISSVEAE DAADYYCHQ WSSYPWTFGG GTKLEIKRAD AAPTVSIFPP SSEQLTSGGA SVVCFLNNFY PKDINVKWKI DGSERQNGVL N SWTDQDSK DSTYSMSSTL TLTK

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Macromolecule #6: methyl alpha-D-mannopyranoside

MacromoleculeName: methyl alpha-D-mannopyranoside / type: ligand / ID: 6 / Number of copies: 1 / Formula: MMA
Molecular weightTheoretical: 194.182 Da
Chemical component information

ChemComp-MMA:
methyl alpha-D-mannopyranoside

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: Quantifoil R2/1 / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 7832 / Average electron dose: 42.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.75 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 163363
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 5

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Atomic model buiding 1

Initial model
PDB IDChain

3jwn

source_name: PDB, initial_model_type: experimental model

15c8

source_name: PDB, initial_model_type: experimental model
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-9me7:
Antibody fragments from mAb21 and mAb824 bound to the adhesin protein FimH containing alpha-methyl mannose

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