EMDB-46494: 3D structure and atomic structure of RS3 of mouse respiratory cilia

Basic information

Entry

Database: EMDB / ID: EMD-46494

• Title: 3D structure and atomic structure of RS3 of mouse respiratory cilia
• Map data: 3D structure and atomic model of RS3 of mouse respiratory cilia

Sample

• Organelle or cellular component: RS3 from mouse respiratory cilia
  • Protein or peptide: x 24 types
• Ligand: x 1 types

• Keywords: axoneme / radial spoke / STRUCTURAL PROTEIN

Function / homology

Function:

radial spoke stalk / negative regulation of stress granule assembly / diiodophenylpyruvate reductase / pseudophosphatase activity / Malate-aspartate shuttle / positive regulation of hepatic stellate cell migration / positive regulation of hepatic stellate cell contraction / radial spoke assembly / nucleoside-phosphate kinase / (2R)-hydroxyphenylpyruvate reductase [NAD(P)H] activity / nucleoside monophosphate kinase activity / regulation of cilium beat frequency / malate dehydrogenase activity / spermatid nucleus elongation / mucociliary clearance / SUMOylation of DNA damage response and repair proteins / sperm flagellum assembly / axonemal central apparatus assembly / radial spoke / Interconversion of nucleotide di- and triphosphates / axonemal dynein complex assembly / vascular associated smooth muscle contraction / inner dynein arm / sperm axoneme assembly / malate-aspartate shuttle / DNA Damage Recognition in GG-NER / Formation of Incision Complex in GG-NER / XPC complex / inner dynein arm assembly / cerebrospinal fluid circulation / epithelial cilium movement involved in extracellular fluid movement / 9+2 motile cilium / cAMP-dependent protein kinase regulator activity / cilium movement involved in cell motility / Formation of the dystrophin-glycoprotein complex (DGC) / smooth muscle contractile fiber / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / positive regulation of hepatic stellate cell activation / DARPP-32 events / cilium movement / photoreceptor connecting cilium / glomerular mesangial cell development / juxtaglomerular apparatus development / dynein heavy chain binding / PKA activation / motile cilium assembly / Vasopressin regulates renal water homeostasis via Aquaporins / GPER1 signaling / heterotrimeric G-protein binding / transcription export complex 2 / Hedgehog 'off' state / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / adenylate kinase / cilium organization / Smooth Muscle Contraction / Anchoring of the basal body to the plasma membrane / Recruitment of NuMA to mitotic centrosomes / (S)-malate dehydrogenase (NAD+, oxaloacetate-forming) / AURKA Activation by TPX2 / L-malate dehydrogenase (NAD+) activity / Factors involved in megakaryocyte development and platelet production / AMP kinase activity / High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells / Regulation of PLK1 Activity at G2/M Transition / dynein axonemal particle / malate metabolic process / NADP+ metabolic process / nuclear pore nuclear basket / nucleoside-diphosphate kinase / flagellated sperm motility / cell projection organization / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / glyceraldehyde-3-phosphate biosynthetic process / dynein complex / oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor / microtubule motor activity / minus-end-directed microtubule motor activity / nucleoside diphosphate kinase activity / dynein light intermediate chain binding / motile cilium / cAMP-dependent protein kinase complex / nucleus organization / ciliary base / protein kinase A regulatory subunit binding / mesenchyme migration / protein serine/threonine phosphatase activity / centriole replication / dynein intermediate chain binding / basement membrane / axoneme / mRNA transport / glial cell projection / single fertilization / cellular response to transforming growth factor beta stimulus / sperm flagellum / cAMP binding / intercellular bridge

Domain/homology:

A-kinase anchor protein 28kDa / Uncharacterised protein family UPF0728 / : / 28 kDa A-kinase anchor / Uncharacterised protein family UPF0728 / MORN repeat-containing protein 5 / : / : / : / Ciliogenesis-associated TTC17-interacting protein, N-terminal domain / : / CFAP61 dimerisation domain / : / Echinoderm microtubule-associated protein second beta-propeller / Cilia- and flagella-associated protein 61, N-terminal domain / Cilia- and flagella-associated protein 61 / Cilia- and flagella-associated protein 61, N-terminal domain / Malate dehydrogenase, NAD-dependent, cytosolic / : / : / Axonemal dynein light chain / Axonemal dynein light chain / : / Echinoderm microtubule-associated protein first beta-propeller / Cilia- and flagella-associated protein 91 / CFAP91 domain / : / Cilia- and flagella-associated protein 91 / Malate dehydrogenase, type 2 / Dpy-30 motif / Dpy-30 motif / Dynein heavy chain 3, AAA+ lid domain / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / : / AAA+ lid domain / Malate dehydrogenase, active site / Malate dehydrogenase active site signature. / : / cAMP-dependent protein kinase regulatory subunit / : / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / MORN motif / : / MORN repeat / Adenylate kinase/UMP-CMP kinase / Quinoprotein amine dehydrogenase, beta chain-like / Adenylate kinase / Dual specificity phosphatase, catalytic domain / MYND finger / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Zinc finger MYND-type signature. / Zinc finger, MYND-type / Zinc finger MYND-type profile. / Rhodanese Homology Domain / Dual specificity protein phosphatase domain profile. / Dual specificity protein phosphatase domain / Leucine-rich repeat / Rhodanese-like domain superfamily / Tetratricopeptide repeat / Rhodanese-like domain / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / Leucine Rich repeat / L-lactate/malate dehydrogenase / Lactate/malate dehydrogenase, N-terminal / Lactate/malate dehydrogenase, C-terminal / lactate/malate dehydrogenase, NAD binding domain / lactate/malate dehydrogenase, alpha/beta C-terminal domain / Cyclic nucleotide-binding domain signature 2. / Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal / P-loop containing dynein motor region / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / ATP-dependent RNA helicase DEAD-box, conserved site / Cyclic nucleotide-binding domain signature 1. / Cyclic nucleotide-binding, conserved site / Leucine-rich repeat, SDS22-like subfamily

Component:

Zinc finger, MYND domain containing 12 / Ciliogenesis-associated TTC17-interacting protein / Dynein, axonemal, heavy chain 6 / Cilia- and flagella-associated protein 44 / Cilia- and flagella-associated protein 251 / Cilia- and flagella-associated protein 43 / Dynein axonemal heavy chain 1 / Tetratricopeptide repeat protein 29 / Adenylate kinase 7 / Adenylate kinase 9 / Leucine-rich repeat-containing protein 23 / cAMP-dependent protein kinase type II-alpha regulatory subunit / Malate dehydrogenase, cytoplasmic / Actin, aortic smooth muscle / UPF0728 protein C10orf53 homolog / A kinase (PRKA) anchor protein 14 / Putative malate dehydrogenase 1B / Cilia- and flagella-associated protein 91 / Axonemal dynein light intermediate polypeptide 1 / Cilia- and flagella-associated protein 61 / Cilia- and flagella-associated protein 57 / MORN repeat-containing protein 5 / Serine/threonine/tyrosine interacting-like 1 / Centrin-2

• Biological species: Mus musculus (house mouse)
• Method: single particle reconstruction / cryo EM / Resolution: 4.7 Å
• Authors: Yanhe Z / Xuewu Z / Daniela N

Funding support

United States, 3 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01GM083122	United States
Cancer Prevention and Research Institute of Texas (CPRIT)	RR140082	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R35GM130289	United States

• Citation: Journal: Nat Struct Mol Biol / Year: 2025; Title: Mouse radial spoke 3 is a metabolic and regulatory hub in cilia.; Authors: Yanhe Zhao / Kangkang Song / Amirrasoul Tavakoli / Long Gui / Angeles Fernandez-Gonzalez / Song Zhang / Petras P Dzeja / S Alex Mitsialis / Xuewu Zhang / Daniela Nicastro / ; Abstract: Cilia are microtubule-based organelles that have important roles in cell sensing, signaling and motility. Recent studies have revealed the atomic structures of many multicomponent ciliary complexes, elucidating their mechanisms of action. However, little is known about the structure, proteome and function of full-length radial spoke 3 (RS3), a conserved complex that transmits mechanochemical signals to coordinate ciliary motility. Here, we combined single-particle cryo-electron microscopy, cryo-electron tomography, proteomic analysis and computational modeling to determine the three-dimensional structure and atomic model of RS3 from mouse respiratory cilia. We reveal all RS3 components, including regulatory and metabolic enzymes such as a protein kinase A subunit, adenylate kinases (AKs) and malate dehydrogenases. Furthermore, we confirm RS3 loss in AK7-deficient mice, which exhibit motility defects. Our findings identify RS3 as an important regulatory and metabolic hub that maintains sufficient adenosine triphosphate for sustained ciliary beating, providing insights into the etiology of ciliopathies.

History

Deposition	Aug 8, 2024	-
Header (metadata) release	Aug 13, 2025	-
Map release	Aug 13, 2025	-
Update	Feb 25, 2026	-
Current status	Feb 25, 2026	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_46494.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: 3D structure and atomic model of RS3 of mouse respiratory cilia
• Voxel size: X=Y=Z: 2.158 Å

Density

Contour Level	By AUTHOR: 0.0037
Minimum - Maximum	-0.0024741674 - 0.031612538
Average (Standard dev.)	0.000035848905 (±0.0005232985)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 776.88 Å α=β=γ: 90.0 °

Supplemental data

Sample components

Entire : RS3 from mouse respiratory cilia

• Entire: Name: RS3 from mouse respiratory cilia

Components

• Organelle or cellular component: RS3 from mouse respiratory cilia
  • Protein or peptide: Adenylate kinase 9
  • Protein or peptide: Leucine-rich repeat-containing protein 23
  • Protein or peptide: Serine/threonine/tyrosine interacting-like 1
  • Protein or peptide: Cilia- and flagella-associated protein 91
  • Protein or peptide: Actin, aortic smooth muscle
  • Protein or peptide: Axonemal dynein light intermediate polypeptide 1
  • Protein or peptide: Centrin-2
  • Protein or peptide: Dynein axonemal heavy chain 1
  • Protein or peptide: Cilia- and flagella-associated protein 57
  • Protein or peptide: Cilia- and flagella-associated protein 43
  • Protein or peptide: Cilia- and flagella-associated protein 44
  • Protein or peptide: Dynein, axonemal, heavy chain 6
  • Protein or peptide: MORN repeat-containing protein 5
  • Protein or peptide: Cilia- and flagella-associated protein 61
  • Protein or peptide: Cilia- and flagella-associated protein 251
  • Protein or peptide: Tetratricopeptide repeat protein 29
  • Protein or peptide: Zinc finger, MYND domain containing 12
  • Protein or peptide: Malate dehydrogenase, cytoplasmic
  • Protein or peptide: Putative malate dehydrogenase 1B
  • Protein or peptide: UPF0728 protein C10orf53 homolog
  • Protein or peptide: A kinase (PRKA) anchor protein 14
  • Protein or peptide: cAMP-dependent protein kinase type II-alpha regulatory subunit
  • Protein or peptide: Ciliogenesis-associated TTC17-interacting protein
  • Protein or peptide: Adenylate kinase 7
• Ligand: ZINC ION

Supramolecule #1: RS3 from mouse respiratory cilia

• Supramolecule: Name: RS3 from mouse respiratory cilia / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#24
• Source (natural): Organism: Mus musculus (house mouse)

Macromolecule #1: Adenylate kinase 9

• Macromolecule: Name: Adenylate kinase 9 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: adenylate kinase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 219.835375 KDa

Sequence

String:

MASEEKTEEL HPFTDIFNED ETDRNCLLSK PTCFIIFGKP GAGKTTLARN IAQAWKCIRV EALSVLEEHI AAEKETGAML QSLLVSGHS IPDELVTKLI LEKIKSPEVA HFGYILTEMP SLAQDNMTSL KQIELVKNLE LQPDIIINIK CSDYDLCQRT C GQRQHSTT GYVYTREQWD PEIIESRRRK KRDFPKEGKS EEEEEEEEQE EEEAFIAEMQ MVAEILQHLV QRPEDYLENV EI TVKLYKE LLLSALEEVM AEHNPQYLIE LDGNKSPEEL FMTVIERLKY LNVRRAAVIT KLQGTEEEMT DIIDTEELFR TVS SYKLIA PRYRWYRSKW ARSCPVSLKD GNIYSGAADY TVSFLGKMYC LSSEETLKLF SLNPRPFLLP PMPLPPCKVF IFGP EHIGK TTLANLIAEH FKAKVIDYAK LVQPRFDSAR EKLIKDTITE ATNTAIKVVQ QRLLNEKQAK QQEERTLKEL QVQYS KREY NDFLSKSSEE LPSLENTGSK LSSLEIGQED KSKSETTITG DQVKDVSTEE SIEEVTENHP EVFAILEDTL RHAKEL NFE QPHDKQAEIL EEVIKETEDN KNRFPGAPKH GGWIVENFPL VRELWLSLIE KALLPDLVVY LSDAESNGKH VLNRLYL KN KAEIDGKILE RLQEEAQAKK REEEEIRKVK EEELRLEEEK QRLMELATKK SKGRHRLTTL VAHHLALPYP DYPDNEAE E EVEDSEIHEE SEAQEIPEYT RGSSLPEASE TSETPEGDHE PEAEFKPPGD TAVEAETEKD PKEGLGSEDL LKVMLPEFP EDGYPNVPEM EPLKEVLNNH ILSWKQLEQT IMDNVVSTLT VDVSNKTPQE LLQRVVETME RPFKYSAWEL NAEDYEEEAE DYQAETEID EEQEEEEEEE EEGEEKIKEK RRHMGDTKHF CPVVLKENFV LQPGNPDEAV KYREKIYYFS STEAKEKFLE H PEDYVSQN EPLKAPPLRI CLLGPHGSGK TVCARKLAEN FGIFHIQFDE FLQEKMLLKA ERKFGPEFED DSEEEQLVKQ EL EELAAQA NVKIEEDTTK KQLPDVQLTE EEEAIKLSLT DNEPLPSEIL DSILSEWWLK EPIRSTGFIL DGFPRHPEEA QFL GERGFF PDAAVIIQVD DQDIFDRLLP AQVQKWKTKQ HKKLERKKLI KDLKTKIKED MVAKRRAELI LEREKKRRED GTFR DEDEF SDEDADYEDD IENILEEEFP KDEEEMSEED EEQEADATER LRNELGEKFE TETNNIQSIQ DEFDKVLIPI IMVNG ARKM HIVQYVMNMK LKPLVENRES IFEKCYPVSS HLAHKMLSYT YKHMSSFGYW DPVKLSEGET IKPVENAENP LNPVIH RHY IYFLSNKQTK EKFMMNPIKY IRQPKPKATM PVRIMIVGPP KSGKTTVAKK LASDYGLRCL SVGDALRGML NNHPDSE LS LMLNWHLHKG KTVPDELAIQ ALDISLMESV CNTIGVVIDG YPVTPHQMDL LEARSIIPMV IFELHVPSKE IFKRLLLE K KTEQSMSYPL HNSSQIIAYK NAKYHKNINE IRQFYQKQHQ NWHVIDGFHS KWWVWNEVLK DIQQMNKYIQ TYMKKIKEG KAACIDKLCI TPEELIARLG EFKQFCPVSL AESNELIDCS VTSSLEFAAE FRGHYYKMSS QEKLNKFLKD PELYVPPLAP HPLPPDDLL PKRLTLSELK SRFPKCAELQ GYCPVTYQDG KQRYEALVPG NIRYALEYRN CIYICESEEK LQKFMRSPMQ Y WNQKLPRK LPPLKESIHL TSLPLPGYLE QGTATSLIKA MNAAGNLKPK LPFLSVRRSV LLYIAFHLKA FNPKGSEYSR KK YKKKLEQ FVERCELITY LSSKMTRKYK EPQFRAIDFD HKLQTFLSLK NIDPVTG

UniProtKB: Adenylate kinase 9

Macromolecule #2: Leucine-rich repeat-containing protein 23

• Macromolecule: Name: Leucine-rich repeat-containing protein 23 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 39.337973 KDa

Sequence

String:

MSDEDDVDDV DAEQDEVESD KEIEEWEDYR KETEEASEEW LPTPITEAMM KEGLSLLCKI GSGLAHAYIK LEAKDRDLTD ISLLRSYIH LRYVDISENH ITDISPLNSL THLLWLKADG NQLRSARMNE LPYLQIASFS YNQIIDTEGI FHPRLGSLDL K GNRIHQVT GLDPERLSSL HTLELRGNQL ESTKGIYLPK LKNLYLAQNL LKKVEGLENL SNLTTLHLRD NQIETLNGFS QE MKSLQYL NLRSNMISDL AELAKLRDLP KLRALVLLDN PCADETDYRQ EALVQMAHLE RLDKEFYEDD DRAEAEEIRQ RLK EEQDQD LDPDQDMEPY LPPV

UniProtKB: Leucine-rich repeat-containing protein 23

Macromolecule #3: Serine/threonine/tyrosine interacting-like 1

• Macromolecule: Name: Serine/threonine/tyrosine interacting-like 1 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 37.712379 KDa

Sequence

String:

MADLLFCEPT ELYNILNQVS KLSRLAEPNY LCLLDVRSKR QYDESHVITA LRVKKRDHQY LIPESVDLEC VRYCIVYDSN TSSLELCIR RQLEEEEEEE EVEREDKEED TELLPGPAVE FGQILIHFTR QPVYVLRGGY ECFSGMYHFF RTQKIIWMPQ E LDAFQPYP VEILPGRVYL GKISQACNAK MHKDLKIKAH VNISMETTPY FIGNADKLLH IKLEDSPDTL LFDYFRHICH FI ELHLRLH SVVLVFSTRG ISRSVAAVVA LLMHYNEETL KRSWAQVKKC KTNMRPNRAF VAQLLEWEKV LHGEYLTDIS EPI Y

UniProtKB: Serine/threonine/tyrosine interacting-like 1

Macromolecule #4: Cilia- and flagella-associated protein 91

• Macromolecule: Name: Cilia- and flagella-associated protein 91 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 91.764516 KDa

Sequence

String:

MSQTVTIQEP RPNDQRIPYQ CREVRRAKGG FANRTYDYLY DPLFIVSSER DHAQANIQAT LIRSRLKKVP NFRSMFSNLF HHPRYSMYW SKTDPVPLHV TREWRGQEAK HKEVLRLQAA MDTSFQMPKE KDEDPDVSGK NRYKFFDRPF LPFLQQMPLN V VLSPVKTQ PLLLTPESSK YAIIPTKSTV ATQTDYRDAD VQTDPYSPEY VVCQDTIPEL LTLANLTWGR GLPAGQAEVE II ERAREKR AWEATLPPLN DSVQAEKRRK MMNAMERKEW AFREGEIEKL QELRLEVLKQ LLKRREEDQN ELNMRHLNDQ WYK LQEAKE AKVAQIRHKH VSDIRKLMGK GKNIEGKLQR RDIISDYSNF ASQVYGPLSR LGRFPDNNSE DFVVRNHYLN TYEG LVELE SSLPDFVTQP RIKPPKPQII TTKAGFLKRT ARMDYELAEV HKALVDKKNK GLEGTKSLRF LQKNPISQAR LPTPS LEMT SYEEGEIEMA VIYLQKLLRG RVVQNMMFEG KEKRLELILE LRTSHALQED DKLVKKAEKQ VTLALQRQRN LHEDKL SVI ENHLGDLEGR VLADMFDFLS KELVRLQEER RIHAFAMLAE RQRRMREAEE SGRRQVEQKR LQQEDMIFKE VIKVHQS TV TSYLEDIILN TEERTAEEQA RKEIEKIAEE INNIAYEMEN RRTYLQSEEI VAELVYSFLI PEVQKDFVKE KVRNAQRK H ILAAHEIIHS NTETMLEEQV YKELQSEDFE LEEEAESLDS EVPTVSVSKT STIKPTQDEG EG

UniProtKB: Cilia- and flagella-associated protein 91

Macromolecule #5: Actin, aortic smooth muscle

• Macromolecule: Name: Actin, aortic smooth muscle / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO; EC number: Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 42.054875 KDa

Sequence

String:

MCEEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWH HSFYNELRVA PEEHPTLLTE APLNPKANRE KMTQIMFETF NVPAMYVAIQ AVLSLYASGR TTGIVLDSGD G VTHNVPIY EGYALPHAIM RLDLAGRDLT DYLMKILTER GYSFVTTAER EIVRDIKEKL CYVALDFENE MATAASSSSL EK SYELPDG QVITIGNERF RCPETLFQPS FIGMESAGIH ETTYNSIMKC DIDIRKDLYA NNVLSGGTTM YPGIADRMQK EIT ALAPST MKIKIIAPPE RKYSVWIGGS ILASLSTFQQ MWISKQEYDE AGPSIVHRKC F

UniProtKB: Actin, aortic smooth muscle

Macromolecule #6: Axonemal dynein light intermediate polypeptide 1

• Macromolecule: Name: Axonemal dynein light intermediate polypeptide 1 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 29.723953 KDa

Sequence

String:

MIPPADSLLK YDTPVLVSRN TEKRSPTARP LKVTIQQPGP SSTSPQPPKA KLPSTSCVPD PTKQAEEILN AILPPREWVE DTQLWIQQV SSTPSTRMDV VHLQEQLDLK LQQRQARETG ICPVRRELYS QCFDELIREV TINCAERGLL LLRVRDEIHM T IAAYQTLY ESSVAFGMRK ALQAEQGKSD MERKITELET EKRDLERQVN EQKAKCEATE KRESERRQVE EKKHNEEIQF LK RTNQQLK AQLEGIIAPK K

UniProtKB: Axonemal dynein light intermediate polypeptide 1

Macromolecule #7: Centrin-2

• Macromolecule: Name: Centrin-2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 19.827562 KDa

Sequence

String:

MASNFKKTTM ASSAQRKRMS PKPELTEDQK QEIREAFDLF DADGTGTIDI KELKVAMRAL GFEPKKEEIK KMISEIDKEG TGKMNFSDF LTVMTQKMSE KDTKEEILKA FKLFDDDETG KISFKNLKRV AKELGENLTD EELQEMIDEA DRDGDGEVNE Q EFLRIMKK TSLY

UniProtKB: Centrin-2

Macromolecule #8: Dynein axonemal heavy chain 1

• Macromolecule: Name: Dynein axonemal heavy chain 1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 487.618844 KDa

Sequence

String:

MEECNKEGPS SSSQGPGYCP VKVPESHDLE KILQESNYHP ERNPLNPDPK TPPLPLTDLR QPRKSPLTGT DKKYPLMKQR GFYSDILSP GTLDKLGNVC CGPYMSQNLI RQADLDKFTP KVDSFVIPED FQERVEQQII GATTRLLTQT DFPLQSYEPK V QVPFQVLP GQCPRKIEIE RRKQQYLRLD IEQLLTSEGI DSNKLMPRHP DLQHPQTIEQ GRDPLFPIYL PLKVFDNEEF DC RTPTEWL NMGLEPGSQN RKPVPGKALL PTDDDLGHED PKNQELDYRW CEVGVLDYDE EKKLYLVQKT DKRGLVRDEM GMP ILNGGI TPAGRPPLLA TQYWVPRIQL LFCAEDPRVF TQRVVQANAL RKYTEALLMY NLYVDCMPTE GRRVINEQSL SKIK QWALS TPRMRKGQSV LEHLSCLARE VNLDYERSMN KINFDQIVSS NPETFSYVTL PEKEEEKVPN QGLVSVPEYP FREQK EDFT FVSLLTRPEV ITALSKVRAE CNKVTSMSLF HSSLSKYSRL EEFEQIQSQT FSQVQMFLKD SWISTLKVAM RGSLRD MSK GWYNLYETNW EVYLMSKLRK LMELIKYMLQ DTLRFLVQDS LGSFTQFIGD ACCSVLECVD DMDWGEDLVN SPYKPRK NP LFIVDLVLDN SGVHYSTPLE HFEMILLNLF DKGILATHAV PQLEKLVMED IFISGDPLLE SVGLHEPLVE ELRANITN A MHKAMMPLQA YAKEYRKYLE LNNNDISTFL KTYQTQCPSA EEVREVVITH LKEKEILDNS LPSSIIIGPF YINVDNVKQ SLSKKRKALA TSMLDILAKN LHKEVDSICE EFRSISRKIY EKPNSIEELA ELRDWMKGIP EKLVFLEERI VKVMSDYEVM DEFFYNLTT DDFNDKWAAN NWPSKILGQI DMVRQQHVED EEKFRKIQLM DQNNFQEKLE GLQLVVAGFS IHVEIARAHE I ANEVRRVK KQLKDCQQLA MLYNNRERIF GLPITNYDKL SRMVKEFQPY LDLWTTASDW LRWSESWMND PLSAIDAEQL EK NVIESFK TMHKCVKQFK DIPACQEVAL DIRTRIEEFK PYIPLIQGLR NPGMRNRHWE VLSNEININV RPKANLTFAR CLE MNLQDH IESISKVAEV AGKEYAIEQA LDKMEKEWSS ILFNVLPYKE TDTYILKSPD EASQLLDDHI VMTQSMSFSP YKKP FEQRI NSWETKLKLT QEVLEEWLNC QRAWLYLEPI FSSEDITRQL PVESKRYQTM ERIWRKIMKN AYENREVINV CSDQR LLDS LRDCNKLLDL VQKGLSEYLE TKRTAFPRFY FLSDDELLEI LSQTKDPTAV QPHLRKCFEN IARLLFQEDL EITHMY SAE GEEVKLSFSI YPSSNVEDWL LEVERSMKAS VHDIIEMAIK AYPTMLRTEW VLNWPGQVTI AGCQTYWTME VAEALEA GN ISSKLFPQLS KQLSDLVALV RGKLSRMQRM VLSALIVIEV HAKDVVSKLI DENVVSVHDF EWISQLRYYW TKDDLYIR A VNAEFIYGYE YLGNSGRLVI TPLTDRCYLT LTGALHLKFG GAPAGPAGTG KTETTKDLGK ALAIQTVVFN CSDQLDFMA MGKFFKGLAS AGAWACFDEF NRIDIEVLSV VAQQITTIQK AQQQRVERFM FEGVEIPLVP SCAVFITMNP GYAGRTELPD NLKALFRPV AMMVPDYAMI AEISLYSFGF NEANVLAKKI TTTFKLSSEQ LSSQDHYDFG MRAVKTVISA AGNLKRENPT M NEELICLR AIRDVNVPKF LQEDLKLFSG IVSDLFPTIK EEETDYGILD QAIRRSCEKN NLKDVEGFLI KCIQLYETTV VR HGLMLVG PTGSGKSNCY RVLAAAMTLL KGKPSISGGV YEAVNYYVLN PKSITMGQLY GEFDLLTHEW TDGIFSSLIR AGA IASDTN KKWYMFDGPV DAVWIENMNT VLDDNKKLCL SSGEIIKLTE AMTMMFEVQD LAVASPATVS RCGMVYLEPS ILGL MPFVE CWLKRLPAII KPYEEQFKSL FTKYLENSIN FVRNTVKEVI ASTNSNLTMS LLKLLDCFFR PFLPREGLKK IPSEK LSHI PELIEPWFIF SLVWSVGATG DHTSRISFSQ WLRLKMRLEQ VKLGFPEDGL VYDYRLDDAG ISSTEDDDEE EDENKQ VSW VKWMDYSAPF TMMPDTNYCN IIVPTMDTVQ MSYLLGMLLT NHKPVLCIGP TGTGKTLTVS NKLLKYLPLE YISHFLT FS ARTSANQTQD LIDSKLDKRR KGVFGPPLGR NFIFFIDDLN MPALETYGAQ PPIELLRQWM DHGGWYDRKV IGAFKNLV D INFVCAMGPP GGGRNAITPR LTRHFNYLSF IEMDEVSKKR IFSIILECWM DGLLGEKSYR EPVPGAPNID DLTEPLVDA TINVYGIITS QLLPTPAKSH YTFNLRDLSK VFQGMLMAEP SKVEDKVQLL RLWYHENCRV FRDRLVNEED RSWFDELLEA QMEEFGVAF NKVCPFQPIL YGDFMSPGSD VKSYELITSE SKMMQVIEEY MEDYNQINTA KLRLVLFVDA MSHICRISRT L RQALGNAL LLGVGGSGRS SLTRLASHMA EYECFQIELS KNYGMSEWRE DVKKVLLKAG LQNLPITFLF SDTQIKNESF LE DINNVLN SGDIPNIYSA DEQDQIINTM RPYIQEQGLQ PTKANLMAAY TGRVRSNIHM VLCMSPIGEV FRARLRQFPS LVN CCTIDW FNEWPAEALK SVATTFLSEI PELECSEEVI QGLIQVCVFI HQSVASKCVE YLAELARHNY VTPKSYLELL NIFS ILIGQ KKMELKTAKN RMKSGLDKLL RTSEDVAKMQ EELEIMRPLL EEAAKDTMLT MEQIKVDTAI AEETRKSVQA EEIKA NEKA NKAQAIADDA QKDLDEALPA LDAALASLRN LNKNDVTEVR AMQRPPPGVK LVIEAVCIMK GIKPKKVPGE KPGSKV DDY WEPGKGLLQD PGRFLESLFK FDKDNIGEAV IKAIQPYIDN EEFQPAAIAK VSKACTSICQ WVRAMHKYHF VAKAVEP KR QALREAQDDL EVTQRILEEA KHHLHEVEDG IATMQAKYRE CVAKKEELEM KCEQCEQRLG RADKLINGLA DEKVRWQE T VENLENMLDN IFGDVLVAAG FVAYLGPFTG QYRTTLYEYW VNQLTVHHVP HTSKPTLITT LGNPVKIRSW QIAGLPNDT LSVENGVINQ FSQRWTHFID PQGQANKWIK NMERESGLDV FKLSDRDFLR SMENAIRFGK PCLLENVGEE LDPALEPVLL KQTYKQQGN IVLKLGDTVI PYHEDFRMYI TTKLPNPHYS PEISTKLTLI NFTLSPSGLE DQLLGQVVAE ERPDLEEAKN Q LIVSNAKM RQELKDIEDQ ILYRLSSSEG NPVDDMELIK VLEASKMKAA EIQAKVRIAE QTEKDIDLTR MEYIPVAVRT QI LFFCVSD LANVDPMYQY SLEWFLNIFL SGIANSERAD NLKKRIVNIN RYLTFSLYSN VCRSLFEKHK LMFAFLLCVR IMM NEGKIN QAEWRYLLSG GSIQTMFENP APQWLSDRAW RDILALSNLP TFATFSNDFV MYLSEFQAIF DSAEPHRELL PGIW NAYLD EFQKLLILRC LRGDKVTNAM QDFVATHLEP RFIEPQTANL SAVFKESNST TPLIFVLSPG TDPAADLYKF AEEMK FSKK FSAISLGQGQ GPRAEAMMRN SIERGKWVFF QNCHLAPSWM PALERLIEHI NPDKVHRDFR LWLTSLPSNK FPVSIL QNG SKMTIEPPRG VKANLLKSYN SLSDDFLHSC QKVVEFKSLL LSLCLFHGNA LERRKFGPLG FNIPYEFTDG DLRICIS QL KMFLDEYEDI PYKVLKYTAG EINYGGRVTD DWDRRCVMNI LEDFYNPAVL SSEHSYSNSG IYHQIPPTYD LNGYLSYI K SLPLNDMPEI FGLHDNANIT FAQNETFALF NAILQLQPKS SSMGGQSREE LVEDVAENIL LQVPGPIELQ EVTKKFPVL YEESMNTVLV QEVIRYNKLL EVITQTLSDM LKAIKGLVVM SLELELMSIS LYNNTVPELW KSKAYPSLKP LASWIMDLLL RLDFMHSWI NDGIPPVFWI SGFFFPQAFL TGTLQNFARK FVISIDTITF DFKVLPEASS EIKERPQTGC YIHGLFLEGA R WDSMNFQL AESRPKELYT EMAVIWLLPE ANRKVQNQDF YLCPIYKTLT RAGTLSTTGH STNYVIAVEI PSNQPQRHWI KR GVALICA LDY

UniProtKB: Dynein axonemal heavy chain 1

Macromolecule #9: Cilia- and flagella-associated protein 57

• Macromolecule: Name: Cilia- and flagella-associated protein 57 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 145.133297 KDa

Sequence

String:

MSTVVAQALH VFGLRPHVTN NVFFFDEQII IFPSGNHCVK YNIDQKWQKF IAGSDKSQGM LALAISPNRR YLAISETVQE KPAVTIYEL SSIPCRKRKV LNNFDFQVQK FTSMAFSPDS KYLLTQTSPP DSNLVYWLWE KQKVMAIIKA DSQNNPIYQV S ISSQDNSQ VCITGSGVFK LLRFAEGTLK QINFQRGESS NYLAHAWVSE DRVIVGTDTG KLFLFESGDQ RWETSIMVKE ST SSRSLEV IQESESLIEF PPLSSPVSSV ERMDITTNTQ QQAMPQVFAI AAYSKGFACS AGPGRVLLFE KVEEKDFYRE SRE IRIPTD QQSNDPSQSD KQDVLCLCFS PSEETLIAST NKNQLYSITM SLTEISKGEA AHFEYLLYPL HSASITGLDT CIRK PLIAT CSLDRSVRIW NYESNTLELY KEYQEEAYTV SLHPSGHYIV VGFADKLRLM NLLIDDIRPF KEYSVRGCKE CSFSN GGHL FAAVNGNVIH IFTTTSLENI NILKGHTGKI RSLVWNLDDS KLVSAGTDGA VYEWNLSTGK RETECVLKSC SYNSVT TSP DAKVIFAVGS DQTLKEISDS LILREIPAFD VIYTSITISH SGRMIFVGTS VGTIRAMKYP LSLQKEYNEY QAHAGPV MK MLLTFDDQFL LTVGEDGCLF TWKVFDKDGR GIKREREVGF AEEVLVTKTD MEEKAQIMLE LKTRVEELKM ENEYQLRL K DMNYTEKIKE LTDKFIQEME SLKTKNQVLK TEKEKQDISH RERLEELVDK QTRELQDLEC CNNQKLLLEY EKYQELQLK SQRMQEEYEK QLRDNDETKS QALEELTEFY EAKLQEKTGL LEEAQEDVRQ QLREFEETKK QIEEDEDREI QDIKTKYERK LRDEKESNL RLKGETGIMR KKFSSLQKEI EERTNDIELL KSEQMKLQGI IRSLEKDIQG LKREIQERDE TIQDKEKRIY D LKKKNQEL EKFKFVLDYK IKELKKQIEP RENEIKVMKE QIQEMEAELE RFHKQNTQLE LNITELLQKL RATDQEMRKE QQ KERDLEA LVRRFKTDLH NCVAYIQEPG LLKEKIRGLF EKYVQRADMV EIAGLNSDLQ QEYARQREHL ERNLATLKKK VIK EGELHR TDYVRIMQEN VSLIKEINEL RRELKLTRSQ IYDLESALKV SKKTRSQEVP ESVISKDVVG STSTMRLNEQ EETG RIIEM QRLEIRRLRD QIQEQEQVPG FHTIAGVRLP SIVDSDVDFE VHTK

UniProtKB: Cilia- and flagella-associated protein 57

Macromolecule #10: Cilia- and flagella-associated protein 43

• Macromolecule: Name: Cilia- and flagella-associated protein 43 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 193.674672 KDa

Sequence

String:

MSQDPERDDV TASATASASA SAPASASAHY SGSSLSVRWV QGFPSQNVHF VNDQTICYPS GNFVIFINLE TKKKTVLQCI NGIVGVMAT NVPSEVVAFS DRRFKPVIYI YSFPSLTRKN KLKGDILLDY TLLCFSYCGT YLASYSSLPE FELALWNWEA S AILCKKSN PGMDVSQMSF NPMNWHQMCL SSSSAMSVWT IERSNQEHHF RIRSVKLPLE DATFLNEPDM LFPTTLPKDL IY GPVLPLS AIAGLVGEEA ETFRPKDDIY PLLHPTMHCW TPSSDLYVGC EEGHLLMINT ETLKVTVLQK AEEFPLPDGA PLI NPLTLV YQKDGILASG IDGVIYSFII KDSKYQVKTF LEFDGPVTHL VFSPSYKMLL IQTDKGSVYI YTFGAEMPLD KLLD ACDGK VQAVSFITPG TKYFLTLTSS GEVSTVSLED CNCTSRIFLK TQATALACSP SSPTAAVGTV DGYVYFLNIL DVESP QMIH QAFLSQSPVK IVTYDQRGIF LLVGTEEGNI FVIDARPSKS FQIFGFTETG KDILQISTVS VMESDVVEVL VLYPLP DMG RSRLEYFTLP VMLPEVVPEN FSDERGRLKD DLTHKYLYEV EHTLSSAVLG FTGSKIFGFC SQVPYICSYV MPVKEHT GV LVLKPHQKVQ SKQYGSGTIY LSSHGLWLMT IAKCGILCIR DMFSMETFVR CRSHSHQGRG IQNMKMSLDG QHILVNGK D DNTLVCLKWK RLGANIASEI FEHSRPLVLH LSQTVESESV YLALSRESTN EQQEETTESQ KHLNSDSSEE EAVIDHKMI PWIQQKMEEA IKKEVRIFSP RRKEIKRGIK ELAQVIAMMM EENEKVDIIA KLDEQEFCLD ADELERLHDE CEEEVAKIRK DVEMHNLAQ SYLTELIKEE CWNSMAVKGR ALKCFHIPYV VDNFPMKERT EEELQELSKV MQQKKTEIEC LKLRKEIVEV Q ATTTIAKK HHEEEEEEEE DEERTIKTTS LPNYLLGSLS TDFGADTSLL TSQLDLHSRE EKINQIILLK DIIYNIKRNF NS EFDAAYK QKEIEIARVK EKNVRIAEII SDLELEETVW QPVFEDSEKP ERALVVEDDE ISFKKYIAPW QRAKIKEVVS TYE MERLQQ ARISDERQRG LMDMMGGVLE VKKEDILRMV IPQPPFMAKA DALWSEDERK QFKEYEKKVK ELNEERDKYR KSLE AELKK LQNSIQESTQ NFDDHLKRLF ERRVKAEMVI NQEELKINNI IFSLLLDEEL SSREQFLNNY LLKKQEEKTK TAEAI QKAR EDLDVFKEHH DMLVAEDKIL DRSFKKEFSD ILGHQVDVLY KLFKRRPRVH KQKTQADVTS LVPYGERPGS AKLNKE NLA QLMKSMDELD NINNMPEGLD PSVWEHFCST RRAKVENEYK VKQKAACLLE MTTFLRKRME EDDVVHHEIE KVFHELI RL QDEKVRFQVN LTVQILLKQG QVELENFQLM LEYSDAILIN KNIIEDLNSV IRTQGQKKVA SMMESKEVHK GIYQIEWE H KKMEMEMEDL NQRAWDIEML FFSRDRQKYL NEPNYENVIA IQIGIMEQTI SVIDKTHKKN VENCKKLLKK LGKYSNQKD VANYTLSCNL REELVAVSER QDICNEIGSK LTCEKIARER YDNQLKQQKL LNISKQQAEQ ISILQAEVER LRMKTFPALI PM

UniProtKB: Cilia- and flagella-associated protein 43

Macromolecule #11: Cilia- and flagella-associated protein 44

• Macromolecule: Name: Cilia- and flagella-associated protein 44 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 211.839984 KDa

Sequence

String:

MKEPDDQDTS DGGRSGSRNE GKLAHKSILK SSQDTTADSY TDGEESYLGD DLDLDDMDES SHSSQEYVQA PAPSQEPPVE VKEEPEADV KKVLSETFYY DYPELVSIPY VSSEERIPLY FLTLNHSFGY DCRKRANLQL LDSNTLLYVA GNQMVLLDFK D KTQIYLQS SSGQGIGAIG VHPKKTYFAV AEKGSFPKII IYEYPSLKPY RILRDGAEKA YAYVDFNNEG NLLASVGCHP DY TITIWGW KEEQPILRTK AFSQDVFKVT FNPDNDEQLT TSGSGHIKFW EMAFTFTGLK LQGSLGRFGK TSTSDIEGYA ELP DGKVLS GSEWGNLLLW EGSLIKVELC RTGMKSCHSG SINQIMLDEG EVITAGSDGS VRIWDFETID TADVIDDTGL LEIE PINEL HIDKSVNLFS MIKMNEVGNN FWLAQDANGA IWKLDLSFSN ITQDPECLFS FHSGPIAALA VSPLTYLMAT TAMDC SVRV YDFSSKNPLV HMKFKQGGTS LIWAPRSVSV SASQIVVGFQ DGVVRVLELF DPKGLTVYAG RKKIPDAELH LKYVFK PHT DEVTALAYER DGDILATGSE DKTVFFFDVE KEYKPIGFFN TPGPICQLMW SPASHPEKTL LIICENGYIL ESLCPTI KD VDDQNVITFA IPNVFLRCFH FTSVKSKILR FLEVQRREQQ KMLKEKEKLE RRMKLAEERE AFGEEEIPEE ETSEEGEE E EPPLPEIFMP PTPSPILCGF YSEPGKFWVS LGNYDAGFLY HCQFPSYLHN SDFQKQENEP FDFRVLENTE DNPIRNITF SNDQTMMFCG MTNGAIRVYV LSENDPFLVS LQHYWHFNVH DNNYGSIKSI TSSFDDQYLL TAGEDGNIFV FDIFSEFIVP KGIKAKVPS PRFGIESEAA PEDIEDPKAY SIENARKKRE HDKLMKKVEE LKAHKREQIK ILRNEFWKLL ELNKELPAHM Q FQRTDFNI DAKIHAEIHK KTSLKIEQVE KELAWEKQKH ELGLKKLQDR FREPLESDTI VVYATQSDHQ IASYRLVKPS KY SKLKRPS QSERRQSKME RLEKEGPGKK ESQRDTGGSI SLQEESVLEK GKKFRPRTLS EIMVENQIEK TKKLIQQAER AQF KILQRK KEWEELYKSK PDDDYEDPKD VQAIKEAQTY MGDFNLKTAP DYKIPEHMRI NAAKKEEELG YLDTMAHGKK RYMN KCILS LRDLKLAVIE EIQCLVQELK NIQSSIPASK HMPIPQVPQI YPEEVPERRF QYDEETLLRF QRKQKKRQDK SSSKQ SGTG SGGSAGGGLV GFLKLSSGKE GDLTTRDSLS RSSKASALLE LPKPVEFEKA EPSDAELEIM KRDEVKHLYM QQFLCN RIN ELTVTFDAEL HLLRHQKLKL DTKMKLSDLH HLTLFQEMLL LKNFEKQENI LQERVNSLDK EEQDMQWKIN ETLKEME EK KNEITKLQDQ EKALYAGFQA ALGENNKFAN FLMKVLKKKI KRAKKKEVEG DADEDEESEE SSEEESSLES DEDASGSE D DVFDDSICPT NCDVSLFELA LQLREKRLDI EEALVEEKKI VDNLKKEYDT ISKKVKVVAT NLNAAEEALE AYQREKQQR LNELLVVIPL KLHQIEYMEF GEVPEDLSGT LVFSNHSLDR LQERIVQLQE ENAKQQKLNK ECRERRKLLI REKREMAKTI SKMEETVRE LMISKFGRVI DLEALQTLSV NTTLEELKIK KLRKELSNAK ELRMWEEKIA QVRWDLMMKT KEHTKKLHQM N DLCLEKKK LDSRLNTLQN QQGNAFQGLR KADIVAKQKV TELVQTQLEK ITALKEEIEL LRKKGGLLLP PITPKPKNEM KP MDT

UniProtKB: Cilia- and flagella-associated protein 44

Macromolecule #12: Dynein, axonemal, heavy chain 6

• Macromolecule: Name: Dynein, axonemal, heavy chain 6 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 475.368906 KDa

Sequence

String:

MAFQGTEDEI DLKSIEHQAE ENPILARLNK IKAKGILNYV SLKENEPEPP TLVFRHTTKA QERTRKRQQP VKLEPLPVLK VYQDHKQPE YIYEQNRQKL MSSGILKPSP STADRASIIS LEAQEAVKRK QVHRPSADVF SPSPTKLPRT SIGRRGLFGM R SSTYPTYT FHDREEVIKA NIRDPLQIIK IVHENEHLGF LYMISAVPKS SIEYDTYNLK VVSYENINKN DYYTISKDAV TH VYNDDIE YIEIERWEQE YLYHRELTKI PIFALFRKWK AFSVWRKNVR SKKITGCRKA LRKNLFIVNP YLRPALLKIN EMC YLLSFM GLCYIEKFHT YTLQEFKAAQ IVRLQEVTER LEVFRCAAKD VVRKSCRFAL RAAGFIPDDC EVETYEDYFK ANIA SLVDG PFDLPTYGES EKMTYTEQAS KRHHCMRLTC FIRLNDYLIQ NTLHVLTVNT ASSLLNFFSD KLKRTPSADV IQKWI TEEK PEVTEKKGAP LMEKQEEDES LVPMFLTELI LTVQSLLFEP SLEDFLDGIS GAINHFENTV LSVPNLVPDS YFDAFT SPF INKKVEEKTC GPGPNLSAVF EDDRNFLTII MQIKETIHAA FDSARLYAAT FEKFQLFFKE NESLDLEALK QEEPDVQ FF SSQLEKYHKQ HKDSVALRPT RNVGLLLIDT KQLKEKLIPS PLRCLEVLNS MLPCLSKKKV DAIISEAQDA EYKLEFMP T TTIEYVNSLV FLDEIQERIE TLEEEANVVV QMYKLIEQYQ VPTPPEDFAV FATMKPSITA VRNAIDKSVG DRETSIKQF CLHLGRDLED LNNEVNEVKL LAQDPQILDI SADQEKIKTM LSDLQSVLDD LQKRAFQYKS YQKNFKVEVS KFDALEEVSA ELRLKQLLW DSLSEWDNLQ QEWLKSKFDC LDPEVLNSQV TKYAKFVTQL EKGLPPNSVV PQLKQKVEKM KEKLPVIIDL R NPTLKARH WAAIEQTVDA LLVDTEIPLT LERLSELHVF NFAQEIQDIS GQASGEAALE IILKKVEDSW KTTEFVILPH RD SKDVFIL GGTDDIQVLL DDSTINIATI ASSRYVGPLK SRVDDWQKQL SLFNQTLEEW LNCQRNWLYL ESIFNAPDIQ RQL PAEAKM FLQVDKSWKE IMRKVNRLPN ALRAATQPGL LETFQNNNAL LDQIQKCLEA YLESKRVIFP RFYFLSNDEL LEIL AQTRN PQAVQPHLRK CFDSISKLEF ALMPPTEGKI PGMDTEPEKV FTNDILAMLS PEGERVGLGK GLKARGNVEE WLGKV EEAM FTSLRRLCKA AIADYQGKPR TEWVIAGHPS QVILTISQIM WCRDLTESLE CEGDNVDALE DFEQVNFERL NALAAI VRG NLPKLHRNII TALITIDVHA RDIVSELVQA KVDSADSFDW QRQLRYYWDM DLDNCVARMA LSQYTYAYEY LGACPRL VI TPLTDRCYLC LMGALQLDLG GAPAGPAGTG KTETTKDLAK ALAIQCVVFN CSDGLDYKMM GRFFSGLAQS GAWCCFDE F NRIDIEVLSV IAQQLITIRN AKAAKLSRFM FEGREIKLVM TCAAFITMNP GYAGRTELPD NLKALFRPMA MMVPNYALI AEVILYSEGF ESSKILARKM TQMYKLCSEQ LSQQDHYDFG MRAVKSVLVM AGSLKRENPD LSEDVVLIRA LRDSNLPKFL TDDALLFSG IISDLFPGVQ IPEHDYGILQ STIVEVMINQ NLQPELCMVK KVIQLYETML VRHGVMLVGP TGGGKTTVYQ V LAETLGNL EKLNTDNPFY QPVKTYVLNP KSITMGELYG EVNNVTLEWK DGLMALSVRA AVNDTSEDHK WIISDGPVDA LW IENMNTV LDDNKMLCLA NSERIKLTPQ IHMLFEVQDL RVASPATVSR CGMVFVDPEE LKWMPYVKTW MRTMSKKLND EVQ EYLLNL FNRYVDDGLH FVNKKCTQAI PQVDISKVTT LCCLLESLLL SKDGNILSME QMKLNTVLCQ TFVFCYLWSL GGNL TENYW DSFDTFIRTQ FDDNPDARLP SSGDLWSIHI DFDTKRLDPW ERIIPTFKYS RDVPFFEMLV PTTDTVRYGY LMEKL LAVR HSVLFTGTTG VGKSVIAKGL LNRIQESAGY VPVYLNFSAQ TSSSRTQEII ESKLERKRKN ILGAPGNKQV VIFVDD LNM PRLDRYGSQP PIELLRQYQD FGGFYDRNKL FWKEIQDVTI VSACAPPGGG RNPVTPRFIR HFSMLCLPMP SEHSLKQ IF QAILNGFLAD FPEAVKQTSS NIVEAAVEIY NRMSVDLLPT PAKSHYVFNL RDLSKCVQGI LQCDPGTIRE ELQIFRLF C HECQRVFHDR LINNEDKHYF HLILTEMANK HFGIAIDLEY FLSKPIIFGD FIKFGAEKSD RIYDDLPDME KIENVLQDY LDDYNLTNPK EVKLVFFQDA IEHVSRIARM IRQERGNALL VGVGGTGKQS LTRLAAHICG YKCMQIELSR GYNYDNFHED LRKLYKMAG VEDKDMVFLF TDTQIVVEEF LEDINNILNS GEVPNLFEKD ELEHVLAATR PKAKEVGISE GNRDEVFQYF I SRVRQKLH IVLCMSPVGE AFRSRCRMFP SLVNCCTIDW FVQWPREALL SVSKSFFSSV DTGNDDLREK LSLMCVNVHL SV SHMAERY YNELRRRYYT TPTSYLELIN LYLTMLTEKR KQLVSARDRV KNGLTKLLET NVLVDKMKLD LSALEPVLLQ KSQ DVEALM EKLVVDQESA DQVRNVVQED EAIAKVKAEE TQAIADDAQR DLEEALPALE AANKALDSLD KADISEIRVF TKPP DLVMT VMEAISILLN AKPDWPTAKQ LLGDSNFLRR LLEYDKENIK PQILLKLQKY INNPDFVPEK VEKVSKACKS MCMWV RAMD LYSRVVKEVE PKRQKLRAAQ AELDATMATL KEKQALLKQV EDQIKTLQDK YEKGVNEKES LAKNMALTKA RLIRAG KLT AALGDEQVRW EESIEKFQEE LANIVGNVFI AAACVAYYGA FTAQYRQLLI EWWIESCLAL EIPIDPSFSL INILGDP YE IRQWNTDGLP RDLISTENGI LVTQGRRWPL MIDPQDQANR WIRNKESKSG LKVIKLTDTN FLRILENSIR LGLPVLLE E LREVLDPALE PILLKQTFMS GGRLLIHLGD SDIDYDKSFR FYMTSKMPNP HYLPEVCIKV TIINFTVTKS GLEDQLLSD VVRLEKPELE EQRIQLIVRI NSDKNQLKSI EDKILKLLFT SEGNILDNEE LIDTLQDSKI TSGAIKTRLK EAESTELMIN IARERYRPV ATQGSVMYFV IASLSEIDPM YQYSLKYFKQ LFNTTIETSE KSDNLHERLK ILLQQTLLTA YTNVSRGLFE Q HKLIYSFM LCVDIMREHE QLTEAEWNFF LRGSAGMEKE RPPKPEAPWL PIQMWFSCCD LEESFPIFEG LTKHILLRPI SI KLGSFET YINPPVWEGY PKQRHEEEKD HVWGLDFSSF HKLILVKCCK EEKVVFALTD FVIENLGKQF IETPPVDLAT LYQ DMSNST PLVFILSTGS DPMGAFQRFA RESGYAERVQ SISLGQGQGP IAEKMIKDAM KTGNWVFLQN CHLAVSWMLA MEEL IKTFT DPNQTIKDTF RLFLSSMPCS TFPVTVLQNS VKVTNEPPKG LRANIRRAFT EMTPSFFEEN ILGMKWRKLI FGICF FHAI IQERKKFGPL GWNICYEFND SDRECALLNL NLYCHEGKIP WDALIYITGE ITYGGRVTDT WDQRCLRTVL KRFFSP ETL DDDYTYSDSG IYFAPLADSL QDFKEYIEDL PLIDDPEIFG MHENANLVFQ YKETNTLITT ILEVQPRSSS GGQGKSN DE IVQELVASIQ TRVPESLQME GASESLFVKD PQGRLNSLTT VLGQEVDRFN NLLKLIHISL ETLNKAIAGL VVMSEEME K VYQSFLNNQV PSLWSNTAYP SLKPLGSWVK DLILRTEFVD LWLKRGQPKS FWISGFFFPQ GFLTGTLQNH ARKYNLPID ELSFKYNMIP VYRDQAAVIE SAKDIQFGTE LPMDKELPSP EDGVLVHGMF MDASRWDDKD MVIEDALPGQ MNPMLPVVHF EPKQNYEPV HTLYHSPLYK TGARAGTLST TGHSTNFVVT VLLPSKRISD YWISKGSALL CQLSE

UniProtKB: Dynein, axonemal, heavy chain 6

Macromolecule #13: MORN repeat-containing protein 5

• Macromolecule: Name: MORN repeat-containing protein 5 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 20.105605 KDa

Sequence

String:

MQYTGSQYFG EYINGRMEGS AEYILPTDTR YIGEMKDGMF HGEGTLFFPS GSRFDAIWKK GLVVKGKYTF NDGLQYEDKH WHYCDSYDR RFYTEICYGL KPSGISQLTN MDPPRRIPLG YYDCGDGFYN PTTRVIKDYR NRFLRNADDD EHEWIVRTCR K GWLPMPKQ KS

UniProtKB: MORN repeat-containing protein 5

Macromolecule #14: Cilia- and flagella-associated protein 61

• Macromolecule: Name: Cilia- and flagella-associated protein 61 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 143.458938 KDa

Sequence

String:

MSILTSPRGK VEVVHCRRTE SQDIFCIKNL VRKFTQKLFG RLNIIYLLEK ANLAVTVCND KEEIMAHSIF LDYPNWNVAK QDNWIPLFR ELDKEIPCTP LNTLFMHFFV AVDEYATGCL KEIIRTVFKA VPELYFIFLI VPTYLSLGST LITVFDQVGN I PCLNYNED FAVHICHRHN HYPQLHIRKA RVEDHDDLMP IFMHYDNTLK EIYGEYFLAE LIEAQDKDHH AVVCEVEGKA VG FMSVCTS VNLPLLHECF DLGPFHGFCT PHPDDILKPS REPSLEEGEE KHLKKCSTSL IIREMQIKTT LRFHLTPVRM AKI KNSETE TSASCEEALP TVLGLVSEER LKQDMEKNLS ILSLVDEEDV ASSLSSSLLL SVESSHFRPL YLGDPIAFCI QLFC IDERY EARQVLISNV SLSLDFMSFV FSLFPGKNFC LISVPHLTPE FVLIQNFVKV VPFNNCTLDH DLYVFHRAGL LRSIT IRLA KFLDTAGVEK LVSTLLLSTK ILDDLAQYNE AGRDPDGTAL QVFVAEVADQ IVGIAVIRKE MDIEYIRSHY NIEDFI YFS HHQQEEHGRL NHFALNPIFR HYTKFFLKEI LRLGYKSCLY YPVYPQTREG KLQSSYSHSL TSALHYLVPV RPRRQIV YP LEKLGINAPS KEVSKDQVSF ALNHTNRKLT LEPKVTVNAR IVVVGASSVG ISFLETLVFC SHLKFNNLTL ISTHGLPG K RLLHNEQRKF LASDHCFNDK DYALMSLCSW VNVVVGRMTA IDRAAKQVVV SKSEIVFYDH LILCTGLQYQ VPCPTGADT EQHLTNREVL ELSKQRYTGT VPSNLFILND EEDCVRMLCW LRNSSILTEG KVIVYGNTLD AYTTVETLLN IGMKGSGIYF VHPPTESNI TCINNNDVES AVEDALSAAG VTVFHDALLA QWNHGQHPDP IYNACFTTST KPIRLECSAF FSFYKKNVDY E TFKAFNDA CLVYDGRLVI DTTFHTNDIA IRAAGSLTKF SNRYYSNEWT HSNFSSKEIG FQLAAAMLSL FDPTLEPVTE PP ADLDRLI PMYKGAKIQG GILPGSYHYL HISKPAIPTP LNLQRSQPDF GSDVVTGNAK KGTYFRLYIN NYKLVEAITC FSK EPFPSS NYIRLFGQHE QVLNNLCTRF DDKLIPDLYS YFTEPWCMAL FHDRFIDLKK ELRQILISKQ EEDKPTMEEI AYRL EEEEI NLNEKPQKYL QRVFEDSIYK SLLEKSILDY LHYNQYHLPM YAWPGII

UniProtKB: Cilia- and flagella-associated protein 61

Macromolecule #15: Cilia- and flagella-associated protein 251

• Macromolecule: Name: Cilia- and flagella-associated protein 251 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 148.784344 KDa

Sequence

String:

MSDTEENPLE ESRESGEMEE EKEEKKEEEE EEEEGEKEKE EEEEEEKGKE EEKEEEKEEG KEEEKKEEEE EKGKKEEEEE EEEEGKGKE EEKEEEKEEE EKEEEKEKEE EEEEEGKGKE EEEEGKEEEE EEKEEEEKEE EKEEEEEEEE EKEEYNPSFE E DALQQSLT EGSLSWEESQ EDGEGLEEWV EKEEQREGEE VRGEEESEVR GREEEEGWEE EKSGEEEKSE ESERSKERGG EE EGQEKEE AEHEGEREEG REEEEKEKSV EREEEEEEED TETTETKAGR AKEEKKEKQN KTALVDNIVI SHEPPRSSSS LAD SPEVSQ IYKDNSMKVD DTEEASQKPE DILAQGKDEA RLSLEERRKL FQSKGLSAEE SLVSVSTEDT LFQKEEDSKV YPLS MTWSF GWNSSLPVYY MREDRRVILY TCAHTAIMYD VVRNTQYHLQ GHPNIISCLC VSEDRRWIAT ADEGPDCLII IWDSF TGIP VHTIFDSCPE GNGMRSIAIT RDSKFLATIS DSATQKVCIW KWTLAVETPA CTLELPKEYG FQDNLVFNPA NNKELV SNS KTQAIYYCWF EDKGILAHSA PVLTEKTFNK LVGKFSQSVF HLKLPQVLSA TKEGKLVVWD IHYPSSTSSS AISAFPF IK PRKLVHLQKE AITVLMTIDS YIVTGDIKGN IKFYDHTLSV VNWYSNFKLG AIRTLSFSKT IPSLPTEKSN LPTDCTLR G DLFVVRNFII GTFDATVYHM TVDGTKLEKL FVEPRDAVYA VSCHPYQPLI AVGSVCGMIK VWDFEKKVYL FSRTFEKGL GVQCLTYNPE GALLGAGFTE GTVYILDAMS LENESPEPFK YSKSSVSHCC FSHDSNYMAT ADVNFTVAVY MVVVKNGQRV WEYLARLRS HQNSIQSLLF GVHLDSNEPR LLSLGKDRFL IEYNLVKSCK DHLDVLDVHR TDQGNYPTCM IWYPPLTKEL F LLICNSGY KVKLFNATTK MCRKTLLGPA YGSPIEHAQV LPVKSTLELQ KRYLVFINKD KVGLQILPVD GNPHKTCAIV CH PNGVAGM ALSYDGRFAF TAGGQDRSVV QWKINLGALE AAVSLGGEDL TPFYGLVSGG REGKFYRELE DYFYYSQIRS QGI DTMETR QVSEHICLSE LPFVMRAIGF YPSEEKIEDM FNEIKFSEYV ETGKLIDKIN LPDFLKVYLN HRPPFGNTMD GIQN SFNVL GYTNSEGKKA IRREDFLNLL LTKGEHMTEE EMIDCFSTLF GLTPEGWKSE PAATCINGPE ICLQKELPEE ITAEI FTTE ILGLTLSDSS EQLIQ

UniProtKB: Cilia- and flagella-associated protein 251

Macromolecule #16: Tetratricopeptide repeat protein 29

• Macromolecule: Name: Tetratricopeptide repeat protein 29 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 54.413211 KDa

Sequence

String:

MATFPPLPMT HTRLAILARQ KLPCSSKKIP RAQLIKEKED IDYYLEQNFK GLSKEEVAAH RNSYKKSICV DMLRDGFHKS FTELFALME QWDKLREAAK AQSLFWQQRP LEDQPDKLDN FYHYLTRAEA AERKGYYEEV YNNLYALACY FDNSEDKWVR N HFYERCFN IAQLIKADGG KKEAEAESHM GLLFEEEGEL LKAAEHYEAF HELTHGRLWK DGTGQLLNLV ACESLVRTYR LL SDRMLEN KDYKQAIKIL IKASEIAREG NDRSMEGEAS YYLGLAHLAS GEYETALTVL NRYSEISTSL DDDHGLGRAY EAI AKALQS QGETTEAINY LEKFVTIARN NLQSLDMIRA CTMLGDIYNE KGQYSKASEY FQQAFSTAME LMKTALMDET KVHY GIARA HQMMLAMKGY IESADSNGLN CLLSWKETRT QIEYDPILGE SRRATEDNIY QLPDAEEETR RSPENQ

UniProtKB: Tetratricopeptide repeat protein 29

Macromolecule #17: Zinc finger, MYND domain containing 12

• Macromolecule: Name: Zinc finger, MYND domain containing 12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 41.394336 KDa

Sequence

String:

MNVIYPLAVP KGRRLSCEVC EAPAERMCTA CTVTYYCGPV HQKADWDSIH AKICQLLIPL RTTMPFYNSE EERQHGLQQL QRRQKHLIE FCYTVAQKYL FEGRHEDAVP AALHSLRFRM NVHGLSSVEL VPAYLLLAEA SLGLGRIVQA EEYLSQAQWT V LKSTDCSN ATHSLLHRNL GLLYIAKKNL DEARYHLAND IYFASCAYGT EDIRTSGGYF HLANIFYGLK KMELADTLYT KV SEIWYTY LNSHYQALLE ARSQQTDLLG KQFVNDTGLD EAQEAEAIRI LTSTLIIQES TSTKAPQKSI LILKTLSILY YLM LECSKA KEYAMKALSL AETYVSEEEQ RFIQELLSVI SAEEKQP

UniProtKB: Zinc finger, MYND domain containing 12

Macromolecule #18: Malate dehydrogenase, cytoplasmic

• Macromolecule: Name: Malate dehydrogenase, cytoplasmic / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO; EC number: (S)-malate dehydrogenase (NAD+, oxaloacetate-forming)
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 36.55509 KDa

Sequence

String:

MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLQDVIA TDKEEIAFKD LDVAVLVGS MPRREGMERK DLLKANVKIF KSQGTALEKY AKKSVKVIVV GNPANTNCLT ASKSAPSIPK ENFSCLTRLD H NRAKSQIA LKLGVTADDV KNVIIWGNHS STQYPDVNHA KVKLQGKEVG VYEALKDDSW LKGEFITTVQ QRGAAVIKAR KL SSAMSAA KAIADHIRDI WFGTPEGEFV SMGVISDGNS YGVPDDLLYS FPVVIKNKTW KFVEGLPIND FSREKMDLTA KEL TEEKET AFEFLSSA

UniProtKB: Malate dehydrogenase, cytoplasmic

Macromolecule #19: Putative malate dehydrogenase 1B

• Macromolecule: Name: Putative malate dehydrogenase 1B / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO; EC number: Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 56.356164 KDa

Sequence

String:

MAKFVIAGKA NCPYYAKAEL LADYLQKNLP DFRIFKITQH PDKWEDWVED VCERNMWDHR TSPIIWRELL DRGGRGLLLG GYNEFLEHA QLYYGVTSNM TTELMMVIAK ENMQTHTEQQ LDKETMKDLI SPLQVWIASA GTYVCCHLIP LLLSGEVFGM H TEISLTLF DQEQREDCLR SIVMETQDLA SPVLRTVSFC TTVKEAFLQA QVIIILDDST EEEVYSLESC LRSRVPLCRL YG YLIEKNA HKSVKVIVGG KNFVNLKTTL LMQYAPNIAS NIIAVALGVE GQAKAVLARK MKTTSANIKD VIIWGNITGN NYV DLRKAK VYNYESAVKG PPGHYHSVLS LIFDREWITK EFVQTLKILS STGKQFGGIL AAHSIATTLK YWYHGSPPGE IVSL GVMSE GQFDIPEGIV FSMPVKFENG TWVVLTDLED ISLSEKTLSR LTGDLIQEKL VACGDLLTFQ PIEEDPKDNE PSNTG MNEE KEQPGSDDSD EKNEDQ

UniProtKB: Putative malate dehydrogenase 1B

Macromolecule #20: UPF0728 protein C10orf53 homolog

• Macromolecule: Name: UPF0728 protein C10orf53 homolog / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 10.393749 KDa

Sequence

String:

MPAQAVVTLR YGPYSAVGLS VEHRTYRLQG LQAVLAKDGH QIILEQIEDW NLVELVVNEE TVFQCDIQEL EFGGDGKLDP LCEEARIAV LNAF

UniProtKB: UPF0728 protein C10orf53 homolog

Macromolecule #21: A kinase (PRKA) anchor protein 14

• Macromolecule: Name: A kinase (PRKA) anchor protein 14 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 58.332453 KDa

Sequence

String:

MSNKKKFPNQ KAKIVENTST AKTKKPEAPE PKHVTEPKDS KSVEDPKIPV TDQKEKKTVT TIAEPKDKKS ETEPKEKKTV TISDPKAKK PAIEKDKRTV TFVTEVKDKR IVTDTKENRI ASIDTKEKVP VVVPEVKEKR VQLLDPKEKV AVLPTTPIGS V AHVPHAPP VAHTEHAAPA KPVAPAKPVA PVAPAKPVAP VAPAKPVVPV APAKPVAPAA PVTSVVRAVP AAPVTPVAPV AP AVPVVPA APVAPAAPVV PAAPVAPVAP ALPVAPVAPV APALPVAPAV PVAPAVPVAP VVPEVKQKIP VSEVKGKKEV PLR VPELKE IKASSFAPEI KVKKVGSEIK QKAHVKDVKE KKIVPEVKEK KPVVVKKKDG ASEVARTVVD GVMAAAVEMV EEAR NPIKN IKWLTHGEFT PEKGRKQIEK FVSTWEFQNR WVYYADFIEK KDLIHSYHYI YRVRWSAPTA VRPMARVSAN ALFTI KFNK SKPADMPVDV SYIFENSELL QRPGEIRFRE QWLRDITETK HILLESIPFK VV

UniProtKB: A kinase (PRKA) anchor protein 14

Macromolecule #22: cAMP-dependent protein kinase type II-alpha regulatory subunit

• Macromolecule: Name: cAMP-dependent protein kinase type II-alpha regulatory subunit; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 45.442977 KDa

Sequence

String:

MSHIQIPPGL TELLQGYTVE VGQQPPDLVD FAVEYFTRLR EARRQESDTF IVSPTTFHTQ ESSAVPVIEE DGESDSDSED ADLEVPVPS KFTRRVSVCA ETFNPDEEEE DNDPRVVHPK TDEQRCRLQE ACKDILLFKN LDQEQLSQVL DAMFEKIVKT D EHVIDQGD DGDNFYVIER GTYDILVTKD NQTRSVGQYD NRGSFGELAL MYNTPRAATI IATSEGSLWG LDRVTFRRII VK NNAKKRK MFESFIESVP LFKSLEMSER MKIVDVIGEK IYKDGERIIA QGEKADSFYI IESGEVSILI RSKTKSNKNG GNQ EVEIAH CHKGQYFGEL ALVTNKPRAA SAYGVGDVKC LVMDVQAFER LLGPCMDIMK RNISHYEEQL VKMFGSNLDL MDPG Q

UniProtKB: cAMP-dependent protein kinase type II-alpha regulatory subunit

Macromolecule #23: Ciliogenesis-associated TTC17-interacting protein

• Macromolecule: Name: Ciliogenesis-associated TTC17-interacting protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 59.355293 KDa

Sequence

String:

MGDVTRGEWV VWGLEAGPGE TASLHHPGLE DMLLFFPETL AILSDTGEPQ GELTIEVQRG KYKDDIGILT HCLLVHASSR GFLDKSLCG SSLLGYLNNN LELMEQHSQE FIKFPVLPME RKMSVLKQDG QFVVTRSVKE GEETKTGVSV FPYKTFKGFV S SAANVVLL RVMAWQQSVP SGARFLALDS EGKLCHCTYK SLGFQTIQVG NQQAKMFIVE QTIHSEEGIP FSCQYYLLPD GH LAKRVQV GSPGCCIITK MPLIREEDVI ESPPTFDKKP LVWGEDLELY SKFLDRKEQL RLSHASYLRH HPEAQALVSD FLL FLLLRR PEDVVTFAAE HFRPFAALRS PIPALRSSHQ PSPFRTLENE EEEEAKEEEE KEEEEEEEEE IAGEIEGEEE EEEI EEGDD YLYMDEDEDI EDYTYYENYY YNYDEEVDSY DDVNNYDDDD IANVDDNDDI DNYDDDNDDD DDDDDNDDED DVDDN DDDD VDKVRAKVDN LSVDNDDNDV VNSDNVDVDN DNNNQG

UniProtKB: Ciliogenesis-associated TTC17-interacting protein

Macromolecule #24: Adenylate kinase 7

• Macromolecule: Name: Adenylate kinase 7 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO / EC number: adenylate kinase
• Source (natural): Organism: Mus musculus (house mouse)
• Molecular weight: Theoretical: 82.643609 KDa

Sequence

String:

MAEEEVPATT EKVLKIQRVF INHLDTYSSG NIGKFLANCV AGASLEEIGE EEEEEDETKS AVAPEAPTTK VKEGTFQIVG TLVKPESVK PDFAVEAYKG ISREDLLSHL MECDAVIYNI TENVQQVEEA LWAVSALNEE ISHFEKRKVF ILLSTVMTWA R SKPLDPDD NEVPFTEEDY RRRKHHPNFL DHINAEKIVL KFGKNAKKFA TYVVASGLQY GAEGGILHTF FKMAWLGEVP AL PVFGDGT NCIPAIHVVD LAGVIQNIID HVPKLHYLVA VDEAVHTLED LVKCISKNTG PGKIQKVPKE NAFLTKDLTQ EYL DHLLVN LRMEALFVKE NFNIRWVAQT GFVENINSIL KEYKQSRGLL PIKICILGPP AVGKSSISEE LAKYYKLHHI KMKD VIAEA IAKLEAIVAP KDSVEGEEEG EEEEEEENVD DAQELLDGIK ESMEQNAGRL EDQYIIRFVK EKLKSMPCRN QGFIL DGFP KTYDQAKDLF NQEEEEEEEE IRGKIFPYDK LITPEFVCGL DASDEFLKER VMNLPESVVA GTHYSQDRFL RSLSHY RDI NTDDETVFNY FDELEIHPIH IDVGKLEDAQ NRLAIKQLIK EIGKPRNYGL TDEEKAEEEK KAAEERLAKE AAQTAEL EH KEAMEMAEKI ARWEEWNKRL EEVKREEREL LEVQSVPLRN YLMTYVMPTL MQGLNECCKV RPEDPVDFLA EYLFKNNP E MQ

UniProtKB: Adenylate kinase 7

Macromolecule #25: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 25 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.4 ; Details: 30 mM HEPES pH 7.4, 25 mM KCl, 5 mM MgSO4, 1 mM EGTA, 0.1 mM EDTA
• Grid: Model: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
• Vitrification: Cryogen name: ETHANE; Details: 4 microliters of splayed axoneme sample were applied to glow-discharged Quantifoil R2/2 Cu grids. After removing excess liquid by blotting the grid from the backside with Whatman filter paper for 2-3 s, the grid was immediately plunge-frozen in liquid ethane using a home-made plunge freezer..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number real images: 50000 / Average exposure time: 4.2 sec. / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 81000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 280000 ; Details: particles containing RS3 were manually picked (using the conserved spacing of RSs as guide) centering on the A-tubule of the DMT
• CTF correction: Software - Name: cryoSPARC (ver. 3.3.1) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: INSILICO MODEL; In silico model: For the RS3 head, neck, and stalk, the AlphaFold2 models of mouse were used.; Details: For the RS3 base, the PDB entry 8J07 was used as starting.
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 5); Details: The particles used for the head-neck is 37453, the resolution is 7.1. The particle used for the base-stalk is 63152, the resolution is 4.7.; Number images used: 63152
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 5)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 5)
• Final 3D classification: Software - Name: RELION (ver. 5)