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- PDB-9d2f: 3D structure and atomic model of RS3 of mouse respiratory cilia -

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Entry
Database: PDB / ID: 9d2f
Title3D structure and atomic model of RS3 of mouse respiratory cilia
Components
  • (Adenylate kinase ...) x 2
  • (Cilia- and flagella-associated protein ...) x 6
  • A kinase (PRKA) anchor protein 14
  • Actin, aortic smooth muscle
  • Axonemal dynein light intermediate polypeptide 1
  • Centrin-2
  • Ciliogenesis-associated TTC17-interacting protein
  • Dynein axonemal heavy chain 1
  • Dynein, axonemal, heavy chain 6
  • Leucine-rich repeat-containing protein 23
  • MORN repeat-containing protein 5
  • Malate dehydrogenase, cytoplasmic
  • Putative malate dehydrogenase 1B
  • Serine/threonine/tyrosine interacting-like 1
  • Tetratricopeptide repeat protein 29
  • UPF0728 protein C10orf53 homolog
  • Zinc finger, MYND domain containing 12
  • cAMP-dependent protein kinase type II-alpha regulatory subunit
KeywordsSTRUCTURAL PROTEIN / axoneme / radial spoke
Function / homology
Function and homology information


radial spoke stalk / negative regulation of stress granule assembly / pseudophosphatase activity / Malate-aspartate shuttle / positive regulation of hepatic stellate cell migration / positive regulation of hepatic stellate cell contraction / phosphatase inhibitor activity / diiodophenylpyruvate reductase (NAD+) activity / nucleoside-phosphate kinase / (2R)-hydroxyphenylpyruvate reductase [NAD(P)H] activity ...radial spoke stalk / negative regulation of stress granule assembly / pseudophosphatase activity / Malate-aspartate shuttle / positive regulation of hepatic stellate cell migration / positive regulation of hepatic stellate cell contraction / phosphatase inhibitor activity / diiodophenylpyruvate reductase (NAD+) activity / nucleoside-phosphate kinase / (2R)-hydroxyphenylpyruvate reductase [NAD(P)H] activity / CDP biosynthetic process / radial spoke assembly / nucleoside monophosphate kinase activity / regulation of cilium beat frequency / malate dehydrogenase activity / spermatid nucleus elongation / mucociliary clearance / SUMOylation of DNA damage response and repair proteins / axonemal central apparatus assembly / radial spoke / sperm flagellum assembly / Interconversion of nucleotide di- and triphosphates / axonemal dynein complex assembly / vascular associated smooth muscle contraction / dCMP kinase activity / inner dynein arm / malate-aspartate shuttle / sperm axoneme assembly / UMP kinase activity / regulation of intrinsic apoptotic signaling pathway / inner dynein arm assembly / XPC complex / cerebrospinal fluid circulation / DNA Damage Recognition in GG-NER / epithelial cilium movement involved in extracellular fluid movement / Formation of Incision Complex in GG-NER / sperm fibrous sheath / 9+2 motile cilium / cilium movement involved in cell motility / Formation of the dystrophin-glycoprotein complex (DGC) / smooth muscle contractile fiber / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / positive regulation of hepatic stellate cell activation / DARPP-32 events / cilium movement / juxtaglomerular apparatus development / glomerular mesangial cell development / dynein heavy chain binding / photoreceptor connecting cilium / PKA activation / Regulation of CDH1 Function / motile cilium assembly / Vasopressin regulates renal water homeostasis via Aquaporins / GPER1 signaling / adenylate kinase / transcription export complex 2 / Hedgehog 'off' state / (S)-malate dehydrogenase (NAD+, oxaloacetate-forming) / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / heterotrimeric G-protein binding / Anchoring of the basal body to the plasma membrane / L-malate dehydrogenase (NAD+) activity / cilium organization / Recruitment of NuMA to mitotic centrosomes / AURKA Activation by TPX2 / AMP kinase activity / Smooth Muscle Contraction / Factors involved in megakaryocyte development and platelet production / cAMP-dependent protein kinase regulator activity / dynein complex / High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells / dynein axonemal particle / malate metabolic process / NADP+ metabolic process / Regulation of PLK1 Activity at G2/M Transition / nuclear pore nuclear basket / oxaloacetate metabolic process / nucleoside-diphosphate kinase / nucleotide-activated protein kinase complex / UDP biosynthetic process / flagellated sperm motility / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / glyceraldehyde-3-phosphate biosynthetic process / motile cilium / cAMP-dependent protein kinase inhibitor activity / cell projection organization / beta-2 adrenergic receptor binding / minus-end-directed microtubule motor activity / nucleoside diphosphate kinase activity / dynein light intermediate chain binding / microtubule motor activity / cAMP-dependent protein kinase complex / NAD+ metabolic process / nucleus organization / ciliary base / protein kinase A regulatory subunit binding / protein kinase A catalytic subunit binding
Similarity search - Function
A-kinase anchor protein 28kDa / Uncharacterised protein family UPF0728 / : / 28 kDa A-kinase anchor / Uncharacterised protein family UPF0728 / MORN repeat-containing protein 5 / : / : / : / Ciliogenesis-associated TTC17-interacting protein, N-terminal domain ...A-kinase anchor protein 28kDa / Uncharacterised protein family UPF0728 / : / 28 kDa A-kinase anchor / Uncharacterised protein family UPF0728 / MORN repeat-containing protein 5 / : / : / : / Ciliogenesis-associated TTC17-interacting protein, N-terminal domain / : / CFAP61 dimerisation domain / : / Echinoderm microtubule-associated protein second beta-propeller / Cilia- and flagella-associated protein 61, N-terminal domain / Cilia- and flagella-associated protein 61 / Cilia- and flagella-associated protein 61, N-terminal domain / Malate dehydrogenase, NAD-dependent, cytosolic / : / : / Cfap43 coiled coil / Axonemal dynein light chain / Axonemal dynein light chain / Cilia- and flagella-associated protein 91 / CFAP91 domain / : / Cilia- and flagella-associated protein 91 / : / Echinoderm microtubule-associated protein first beta-propeller / Malate dehydrogenase, type 2 / Dpy-30 motif / Dpy-30 motif / Dynein heavy chain 3, AAA+ lid domain / Possible plasma membrane-binding motif in junctophilins, PIP-5-kinases and protein kinases. / : / AAA+ lid domain / Malate dehydrogenase, active site / Malate dehydrogenase active site signature. / : / cAMP-dependent protein kinase regulatory subunit / : / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / MORN motif / : / MORN repeat / Adenylate kinase/UMP-CMP kinase / Quinoprotein amine dehydrogenase, beta chain-like / Adenylate kinase / Dual specificity phosphatase, catalytic domain / MYND finger / Dual specificity phosphatase, catalytic domain / Dual specificity phosphatase, catalytic domain / Zinc finger MYND-type signature. / Zinc finger, MYND-type / Zinc finger MYND-type profile. / Rhodanese Homology Domain / Dual specificity protein phosphatase domain profile. / Dual specificity protein phosphatase domain / Leucine-rich repeat / Tetratricopeptide repeat / Rhodanese-like domain superfamily / Rhodanese-like domain / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / L-lactate/malate dehydrogenase / Leucine Rich repeat / Lactate/malate dehydrogenase, N-terminal / Lactate/malate dehydrogenase, C-terminal / lactate/malate dehydrogenase, NAD binding domain / lactate/malate dehydrogenase, alpha/beta C-terminal domain / Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal / Cyclic nucleotide-binding domain signature 2. / P-loop containing dynein motor region / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / ATP-dependent RNA helicase DEAD-box, conserved site / Cyclic nucleotide-binding domain signature 1. / Cyclic nucleotide-binding, conserved site
Similarity search - Domain/homology
Zinc finger, MYND domain containing 12 / Ciliogenesis-associated TTC17-interacting protein / Dynein, axonemal, heavy chain 6 / Cilia- and flagella-associated protein 44 / Cilia- and flagella-associated protein 251 / Cilia- and flagella-associated protein 43 / Dynein axonemal heavy chain 1 / Tetratricopeptide repeat protein 29 / Adenylate kinase 7 / Adenylate kinase 9 ...Zinc finger, MYND domain containing 12 / Ciliogenesis-associated TTC17-interacting protein / Dynein, axonemal, heavy chain 6 / Cilia- and flagella-associated protein 44 / Cilia- and flagella-associated protein 251 / Cilia- and flagella-associated protein 43 / Dynein axonemal heavy chain 1 / Tetratricopeptide repeat protein 29 / Adenylate kinase 7 / Adenylate kinase 9 / Leucine-rich repeat-containing protein 23 / cAMP-dependent protein kinase type II-alpha regulatory subunit / Malate dehydrogenase, cytoplasmic / Actin, aortic smooth muscle / UPF0728 protein C10orf53 homolog / A kinase (PRKA) anchor protein 14 / Putative malate dehydrogenase 1B / Cilia- and flagella-associated protein 91 / Axonemal dynein light intermediate polypeptide 1 / Cilia- and flagella-associated protein 61 / Cilia- and flagella-associated protein 57 / MORN repeat-containing protein 5 / Serine/threonine/tyrosine interacting-like 1 / Centrin-2
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.7 Å
AuthorsYanhe, Z. / Xuewu, Z. / Daniela, N.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM083122 United States
Cancer Prevention and Research Institute of Texas (CPRIT)RR140082 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM130289 United States
CitationJournal: Nat Struct Mol Biol / Year: 2025
Title: Mouse radial spoke 3 is a metabolic and regulatory hub in cilia.
Authors: Yanhe Zhao / Kangkang Song / Amirrasoul Tavakoli / Long Gui / Angeles Fernandez-Gonzalez / Song Zhang / Petras P Dzeja / S Alex Mitsialis / Xuewu Zhang / Daniela Nicastro /
Abstract: Cilia are microtubule-based organelles that have important roles in cell sensing, signaling and motility. Recent studies have revealed the atomic structures of many multicomponent ciliary complexes, ...Cilia are microtubule-based organelles that have important roles in cell sensing, signaling and motility. Recent studies have revealed the atomic structures of many multicomponent ciliary complexes, elucidating their mechanisms of action. However, little is known about the structure, proteome and function of full-length radial spoke 3 (RS3), a conserved complex that transmits mechanochemical signals to coordinate ciliary motility. Here, we combined single-particle cryo-electron microscopy, cryo-electron tomography, proteomic analysis and computational modeling to determine the three-dimensional structure and atomic model of RS3 from mouse respiratory cilia. We reveal all RS3 components, including regulatory and metabolic enzymes such as a protein kinase A subunit, adenylate kinases (AKs) and malate dehydrogenases. Furthermore, we confirm RS3 loss in AK7-deficient mice, which exhibit motility defects. Our findings identify RS3 as an important regulatory and metabolic hub that maintains sufficient adenosine triphosphate for sustained ciliary beating, providing insights into the etiology of ciliopathies.
History
DepositionAug 8, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 13, 2025Provider: repository / Type: Initial release
Revision 1.0Aug 13, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Aug 13, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Aug 13, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Adenylate kinase 9
B: Leucine-rich repeat-containing protein 23
C: Leucine-rich repeat-containing protein 23
H: Serine/threonine/tyrosine interacting-like 1
N: Cilia- and flagella-associated protein 91
O: Actin, aortic smooth muscle
P: Axonemal dynein light intermediate polypeptide 1
Q: Axonemal dynein light intermediate polypeptide 1
R: Actin, aortic smooth muscle
S: Centrin-2
U: Dynein axonemal heavy chain 1
W: Cilia- and flagella-associated protein 57
X: Cilia- and flagella-associated protein 57
Y: Cilia- and flagella-associated protein 43
Z: Cilia- and flagella-associated protein 44
T: Dynein, axonemal, heavy chain 6
I: MORN repeat-containing protein 5
J: Cilia- and flagella-associated protein 61
K: Cilia- and flagella-associated protein 251
L: Tetratricopeptide repeat protein 29
M: Zinc finger, MYND domain containing 12
E: Malate dehydrogenase, cytoplasmic
F: Putative malate dehydrogenase 1B
V: UPF0728 protein C10orf53 homolog
a: A kinase (PRKA) anchor protein 14
b: cAMP-dependent protein kinase type II-alpha regulatory subunit
c: Ciliogenesis-associated TTC17-interacting protein
D: Adenylate kinase 7
G: Adenylate kinase 7
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,090,15331
Polymers3,090,02229
Non-polymers1312
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Adenylate kinase ... , 2 types, 3 molecules ADG

#1: Protein Adenylate kinase 9


Mass: 219835.375 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: G3UYQ4, adenylate kinase
#24: Protein Adenylate kinase 7


Mass: 82643.609 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: F8WIC0, adenylate kinase

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Protein , 14 types, 17 molecules BCHORPQSILMEFVabc

#2: Protein Leucine-rich repeat-containing protein 23 / Leucine-rich protein B7


Mass: 39337.973 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: O35125
#3: Protein Serine/threonine/tyrosine interacting-like 1 / Styxl1 protein


Mass: 37712.379 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAR2
#5: Protein Actin, aortic smooth muscle / Alpha-actin-2


Mass: 42054.875 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: P62737, Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement
#6: Protein Axonemal dynein light intermediate polypeptide 1 / Inner dynein arm light chain / axonemal


Mass: 29723.953 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8BVN8
#7: Protein Centrin-2 / Caltractin isoform 1


Mass: 19827.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9R1K9
#13: Protein MORN repeat-containing protein 5


Mass: 20105.605 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9DAI9
#16: Protein Tetratricopeptide repeat protein 29


Mass: 54413.211 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: E9QLU4
#17: Protein Zinc finger, MYND domain containing 12


Mass: 41394.336 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: A2BGJ5
#18: Protein Malate dehydrogenase, cytoplasmic / Aromatic alpha-keto acid reductase / KAR / Cytosolic malate dehydrogenase


Mass: 36555.090 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: P14152, (S)-malate dehydrogenase (NAD+, oxaloacetate-forming), EC: 1.1.1.96
#19: Protein Putative malate dehydrogenase 1B


Mass: 56356.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse)
References: UniProt: Q5F204, Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor
#20: Protein UPF0728 protein C10orf53 homolog


Mass: 10393.749 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q3KNL4
#21: Protein A kinase (PRKA) anchor protein 14


Mass: 58332.453 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q3V0I7
#22: Protein cAMP-dependent protein kinase type II-alpha regulatory subunit


Mass: 45442.977 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: P12367
#23: Protein Ciliogenesis-associated TTC17-interacting protein


Mass: 59355.293 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: B9EKE5

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Cilia- and flagella-associated protein ... , 6 types, 7 molecules NWXYZJK

#4: Protein Cilia- and flagella-associated protein 91 / CFAP91 / AMY-1-associating protein expressed in testis 1 homolog / AAT-1 / MYCBP/AMY-1-associated ...CFAP91 / AMY-1-associating protein expressed in testis 1 homolog / AAT-1 / MYCBP/AMY-1-associated testis-expressed protein 1 / Protein MAATS1


Mass: 91764.516 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8BRC6
#9: Protein Cilia- and flagella-associated protein 57 / WD repeat-containing protein 65


Mass: 145133.297 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q9D180
#10: Protein Cilia- and flagella-associated protein 43


Mass: 193674.672 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: E9Q7R9
#11: Protein Cilia- and flagella-associated protein 44


Mass: 211839.984 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: E9Q5M6
#14: Protein Cilia- and flagella-associated protein 61


Mass: 143458.938 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: Q8CEL2
#15: Protein Cilia- and flagella-associated protein 251 / WD repeat-containing protein 66


Mass: 148784.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: E9Q743

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Antibody , 2 types, 2 molecules UT

#8: Antibody Dynein axonemal heavy chain 1 / Axonemal beta dynein heavy chain 1 / Ciliary dynein heavy chain 1 / mDHC7


Mass: 487618.844 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: E9Q8T7
#12: Antibody Dynein, axonemal, heavy chain 6


Mass: 475368.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: E9Q0B6

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Non-polymers , 1 types, 2 molecules

#25: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: RS3 from mouse respiratory cilia / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1-#24 / Source: NATURAL
Source (natural)Organism: Mus musculus (house mouse)
Buffer solutionpH: 7.4
Details: 30 mM HEPES pH 7.4, 25 mM KCl, 5 mM MgSO4, 1 mM EGTA, 0.1 mM EDTA
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R2/1
VitrificationCryogen name: ETHANE
Details: 4 microliters of splayed axoneme sample were applied to glow-discharged Quantifoil R2/2 Cu grids. After removing excess liquid by blotting the grid from the backside with Whatman filter ...Details: 4 microliters of splayed axoneme sample were applied to glow-discharged Quantifoil R2/2 Cu grids. After removing excess liquid by blotting the grid from the backside with Whatman filter paper for 2-3 s, the grid was immediately plunge-frozen in liquid ethane using a home-made plunge freezer.

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 81000 X / Nominal defocus max: 3000 nm / Nominal defocus min: 1000 nm / Cs: 2.7 mm / C2 aperture diameter: 70 µm
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 4.2 sec. / Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of real images: 50000
Image scansWidth: 5760 / Height: 4092

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Processing

EM software
IDNameVersionCategory
1cryoSPARC3.3.1particle selection
4cryoSPARC3.3.1CTF correction
10RELION5initial Euler assignment
11RELION5final Euler assignment
12RELION5classification
13RELION53D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 280000
Details: particles containing RS3 were manually picked (using the conserved spacing of RSs as guide) centering on the A-tubule of the DMT
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 63152
Details: The particles used for the head-neck is 37453, the resolution is 7.1. The particle used for the base-stalk is 63152, the resolution is 4.7.
Symmetry type: POINT

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