+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-45295 | ||||||||||||
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Title | Structure of the human BOS:human EMC complex in GDN | ||||||||||||
Map data | Sharpened map of human BOS:human EMC complex | ||||||||||||
Sample |
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Keywords | membrane protein biogenesis / membrane protein complex / MEMBRANE PROTEIN | ||||||||||||
Function / homology | Function and homology information multi-pass transmembrane protein insertion into ER membrane / multi-pass translocon complex / extrinsic component of endoplasmic reticulum membrane / inorganic cation transmembrane transporter activity / EMC complex / omegasome membrane / protein insertion into ER membrane by stop-transfer membrane-anchor sequence / magnesium ion transport / cobalt ion transmembrane transporter activity / tail-anchored membrane protein insertion into ER membrane ...multi-pass transmembrane protein insertion into ER membrane / multi-pass translocon complex / extrinsic component of endoplasmic reticulum membrane / inorganic cation transmembrane transporter activity / EMC complex / omegasome membrane / protein insertion into ER membrane by stop-transfer membrane-anchor sequence / magnesium ion transport / cobalt ion transmembrane transporter activity / tail-anchored membrane protein insertion into ER membrane / Miscellaneous transport and binding events / ferrous iron transmembrane transporter activity / magnesium ion transmembrane transporter activity / copper ion transport / regulation of protein complex stability / autophagosome assembly / RHOA GTPase cycle / regulation of protein-containing complex assembly / regulation of signal transduction / positive regulation of endothelial cell proliferation / positive regulation of endothelial cell migration / positive regulation of angiogenesis / ribosome binding / early endosome membrane / carbohydrate binding / angiogenesis / membrane => GO:0016020 / early endosome / protein stabilization / Golgi membrane / endoplasmic reticulum membrane / apoptotic process / Golgi apparatus / endoplasmic reticulum / protein-containing complex / extracellular region / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.6 Å | ||||||||||||
Authors | Nguyen VN / Tomaleri GP / Voorhees RM | ||||||||||||
Funding support | United States, 3 items
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Citation | Journal: To Be Published Title: Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins Authors: Page KR / Nguyen VN / Pleiner T / Tomaleri GP / Wang ML / Guna A / Hazu M / Wang T / Chou T / Voorhees RM | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_45295.map.gz | 257.5 MB | EMDB map data format | |
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Header (meta data) | emd-45295-v30.xml emd-45295.xml | 34.5 KB 34.5 KB | Display Display | EMDB header |
Images | emd_45295.png | 53.7 KB | ||
Filedesc metadata | emd-45295.cif.gz | 10 KB | ||
Others | emd_45295_additional_1.map.gz emd_45295_half_map_1.map.gz emd_45295_half_map_2.map.gz | 248.2 MB 475.3 MB 475.3 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-45295 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-45295 | HTTPS FTP |
-Related structure data
Related structure data | 9c7vMC 9c7uC C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_45295.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Sharpened map of human BOS:human EMC complex | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.832 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: Unsharpened map of human BOS:human EMC complex
File | emd_45295_additional_1.map | ||||||||||||
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Annotation | Unsharpened map of human BOS:human EMC complex | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_45295_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_45295_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Human BOS complex
+Supramolecule #1: Human BOS complex
+Macromolecule #1: ER membrane protein complex subunit 10
+Macromolecule #2: ER membrane protein complex subunit 1
+Macromolecule #3: ER membrane protein complex subunit 2
+Macromolecule #4: ER membrane protein complex subunit 3
+Macromolecule #5: ER membrane protein complex subunit 4
+Macromolecule #6: Membrane magnesium transporter 1
+Macromolecule #7: ER membrane protein complex subunit 6
+Macromolecule #8: ER membrane protein complex subunit 7
+Macromolecule #9: ER membrane protein complex subunit 8
+Macromolecule #10: Nicalin
+Macromolecule #11: BOS complex subunit NOMO2
+Macromolecule #12: Transmembrane protein 147
+Macromolecule #14: 2-acetamido-2-deoxy-beta-D-glucopyranose
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 2.53 mg/mL | ||||||||||||||||||
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Buffer | pH: 7.5 Component:
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 279 K / Instrument: FEI VITROBOT MARK IV | ||||||||||||||||||
Details | Sample solubilized and purified in GDN. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 17978 / Average electron dose: 60.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: DARK FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 105000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |