- EMDB-44464: 80S ribosome bound with angiogenin and complex of eEF1A and Ala-t... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-44464
タイトル
80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla
マップデータ
80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla, main map used for model refinement
試料
複合体: 80S ribosome bound with angiogenin and complex of eEF1A and Ala-tRNAAla
RNA: x 7種
タンパク質・ペプチド: x 78種
キーワード
Angiogenin / RNase / RIBOSOME
機能・相同性
機能・相同性情報
Eukaryotic Translation Elongation / eukaryotic translation elongation factor 1 complex / angiogenin-PRI complex / tRNA-specific ribonuclease activity / negative regulation of translation in response to stress / tRNA-derived small RNA (tsRNA or tRNA-related fragment, tRF) biogenesis / melatonin binding / HSF1 activation / tRNA decay / signaling ...Eukaryotic Translation Elongation / eukaryotic translation elongation factor 1 complex / angiogenin-PRI complex / tRNA-specific ribonuclease activity / negative regulation of translation in response to stress / tRNA-derived small RNA (tsRNA or tRNA-related fragment, tRF) biogenesis / melatonin binding / HSF1 activation / tRNA decay / signaling / cell communication / tRNA export from nucleus / 加水分解酵素; エステル加水分解酵素; 3'-リン酸モノエステル産生エンドリボヌクレアーゼ / Protein methylation / fungal-type vacuole membrane / Adherens junctions interactions / oocyte maturation / hematopoietic stem cell proliferation / negative regulation of protein phosphorylation / homeostatic process / rRNA transcription / basement membrane / actin filament bundle assembly / positive regulation of phosphorylation / translational elongation / RNA nuclease activity / endocytic vesicle / 90S preribosome / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / translation elongation factor activity / negative regulation of protein kinase activity / ovarian follicle development / phagocytic cup / protein-RNA complex assembly / translation regulator activity / rough endoplasmic reticulum / actin filament polymerization / peptide binding / ribosomal small subunit export from nucleus / RNA endonuclease activity / Neutrophil degranulation / positive regulation of endothelial cell proliferation / gastrulation / response to hormone / MDM2/MDM4 family protein binding / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / placenta development / cellular response to amino acid starvation / stress granule assembly / DNA-(apurinic or apyrimidinic site) lyase / ribosomal large subunit biogenesis / positive regulation of protein secretion / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / negative regulation of smooth muscle cell proliferation / small-subunit processome / spindle / cytoplasmic stress granule / cytoplasmic ribonucleoprotein granule / rRNA processing / actin filament binding / GDP binding / positive regulation of canonical Wnt signaling pathway / rhythmic process / regulation of translation / antimicrobial humoral immune response mediated by antimicrobial peptide / heparin binding / cell migration / antibacterial humoral response / large ribosomal subunit / chromosome / actin cytoskeleton / ribosomal small subunit assembly / growth cone / ribosome binding / ribosomal small subunit biogenesis / actin binding / 5S rRNA binding / small ribosomal subunit / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / endonuclease activity / angiogenesis / large ribosomal subunit rRNA binding / killing of cells of another organism / cytosolic large ribosomal subunit / defense response to Gram-negative bacterium / cytoskeleton / perikaryon / response to hypoxia / cytoplasmic translation / cell differentiation / tRNA binding / mitochondrial inner membrane / negative regulation of translation 類似検索 - 分子機能
Translation elongation factor EF1A, eukaryotic/archaeal / : / GTP-eEF1A C-terminal domain-like / : / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Pancreatic ribonuclease / Ribonuclease A, active site ...Translation elongation factor EF1A, eukaryotic/archaeal / : / GTP-eEF1A C-terminal domain-like / : / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / : / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / Pancreatic ribonuclease / Ribonuclease A, active site / Ribonuclease A-domain / Ribonuclease A-like domain superfamily / Pancreatic ribonuclease / Pancreatic ribonuclease family signature. / Pancreatic ribonuclease / Ribosomal protein L6, N-terminal / 40S ribosomal protein SA, C-terminal domain / Ribosomal protein L6, N-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal protein L30e / Ribosomal L15/L27a, N-terminal / Ribosomal protein L28e / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / : / Tr-type G domain, conserved site / Translational (tr)-type guanine nucleotide-binding (G) domain signature. / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / : / Ribosomal protein S12e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S12e / Ribosomal protein L29e / Translation elongation factor EFTu-like, domain 2 / Ribosomal L29e protein family / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S2, eukaryotic / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e, conserved site / : / Ribosomal protein L10e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L6e signature. / Ribosomal protein L44e signature. / 40S Ribosomal protein S10 / 60S ribosomal protein L18a/ L20, eukaryotes / Elongation factor Tu domain 2 / Ribosomal protein L10e / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein S10, eukaryotic/archaeal / : / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / : / Ribosomal protein S7e signature. / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal L40e family / Ribosomal protein L30e signature 1. / Ribosomal protein L36e signature. 類似検索 - ドメイン・相同性
Small ribosomal subunit protein eS32 / Ribosomal protein L36a / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 ...Small ribosomal subunit protein eS32 / Ribosomal protein L36a / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein eL14 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein uS11 / 40S ribosomal protein S24 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / 40S ribosomal protein S4 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL18 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS28 / 40S ribosomal protein SA / Small ribosomal subunit protein eS8 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS21 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein eL22 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Large ribosomal subunit protein eL20 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL19 / Small ribosomal subunit protein eS27 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL28 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS5 / Elongation factor 1-alpha / Angiogenin / Ubiquitin-ribosomal protein eL40 fusion protein / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL37 類似検索 - 構成要素
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM127094
米国
引用
ジャーナル: Nature / 年: 2024 タイトル: Structural mechanism of angiogenin activation by the ribosome. 著者: Anna B Loveland / Cha San Koh / Robin Ganesan / Allan Jacobson / Andrei A Korostelev / 要旨: Angiogenin, an RNase-A-family protein, promotes angiogenesis and has been implicated in cancer, neurodegenerative diseases and epigenetic inheritance. After activation during cellular stress, ...Angiogenin, an RNase-A-family protein, promotes angiogenesis and has been implicated in cancer, neurodegenerative diseases and epigenetic inheritance. After activation during cellular stress, angiogenin cleaves tRNAs at the anticodon loop, resulting in translation repression. However, the catalytic activity of isolated angiogenin is very low, and the mechanisms of the enzyme activation and tRNA specificity have remained a puzzle. Here we identify these mechanisms using biochemical assays and cryogenic electron microscopy (cryo-EM). Our study reveals that the cytosolic ribosome is the activator of angiogenin. A cryo-EM structure features angiogenin bound in the A site of the 80S ribosome. The C-terminal tail of angiogenin is rearranged by interactions with the ribosome to activate the RNase catalytic centre, making the enzyme several orders of magnitude more efficient in tRNA cleavage. Additional 80S-angiogenin structures capture how tRNA substrate is directed by the ribosome into angiogenin's active site, demonstrating that the ribosome acts as the specificity factor. Our findings therefore suggest that angiogenin is activated by ribosomes with a vacant A site, the abundance of which increases during cellular stress. These results may facilitate the development of therapeutics to treat cancer and neurodegenerative diseases.