EMDB-4368: Unique features of mammalian mitochondrial translation initiation...

Basic information

• Entry: Database: EMDB / ID: EMD-4368
• Title: Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome.

Sample

• Complex: mammalian mitochondrial translation initiation complex
  • Protein or peptide: x 84 types
  • RNA: x 5 types
• Ligand: x 9 types

Function / homology

Function:

Hormone ligand-binding receptors / gonadotropin-releasing hormone receptor binding / gonadotropin hormone-releasing hormone activity / G alpha (q) signalling events / Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / mitochondrial transcription / translation factor activity, RNA binding / mitochondrial translational termination / mitochondrial translational elongation / mitochondrial ribosome assembly / translation release factor activity, codon nonspecific / ribonuclease III activity / ribosome disassembly / microprocessor complex / Mitochondrial translation initiation / Mitochondrial protein degradation / mitochondrial large ribosomal subunit / peptidyl-tRNA hydrolase / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial translation / aminoacyl-tRNA hydrolase activity / regulation of translational initiation / organelle membrane / positive regulation of proteolysis / ribosomal small subunit binding / RNA processing / translation initiation factor activity / rescue of stalled ribosome / double-stranded RNA binding / small ribosomal subunit rRNA binding / large ribosomal subunit / cell junction / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / nuclear membrane / negative regulation of translation / nuclear body / rRNA binding / ribosome / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / protein domain specific binding / GTPase activity / mRNA binding / nucleotide binding / synapse / GTP binding / nucleolus / apoptotic process / positive regulation of DNA-templated transcription / mitochondrion / RNA binding / extracellular space / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm

Domain/homology:

Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / : / Elongation factor G domain 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / Cysteine alpha-hairpin motif superfamily / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / : / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S24, mitochondrial / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / Mitochondrial ribosome subunit S24 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / 28S ribosomal protein S25, mitochondrial / : / Pentatricopeptide repeat domain / Ribosomal protein L55, mitochondrial / Mitochondrial ribosomal protein L48 / 39S ribosomal protein L40, mitochondrial / Mitochondrial ribosomal protein L55 superfamily / Mitochondrial ribosomal protein L55 / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein L37, mitochondrial / Mitochondrial ribosomal protein L46 NUDIX / Mitochondrial ribosomal protein L37 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / 39S ribosomal protein L42, mitochondrial / Mitochondrial 28S ribosomal protein S32 / Ribosomal protein L28/L40, mitochondrial / Mitochondrial ribosomal protein L28 / Ribosomal protein 63, mitochondrial / Growth arrest/ DNA-damage-inducible protein-interacting protein 1 / Ribosomal protein L51, mitochondrial / Growth arrest and DNA-damage-inducible proteins-interacting protein 1 / Mitochondrial ribosomal subunit / Mitochondrial ribosome protein 63 / Ribosomal protein L37/S30 / Growth arrest and DNA damage-inducible proteins-interacting protein 1 domain superfamily / : / : / Mitochondrial 28S ribosomal protein S30 (PDCD9) / 39S ribosomal protein L52, mitochondrial / Mitoribosomal protein mL52 / : / Ribosomal protein L35, mitochondrial / MRPL44, double-stranded RNA binding domain / MRPL44 dsRNA-binding domain / Large ribosomal subunit protein mL44, endonuclease domain / : / Large ribosomal subunit protein bL9m C-terminal domain / Tim44-like domain / Tim44-like domain / Tim44 / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / : / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / : / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / Pentatricopeptide repeat / Ribosomal protein L49/IMG2 / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial large subunit ribosomal protein (Img2) / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Ribosomal protein L46, N-terminal / 39S mitochondrial ribosomal protein L46 / Ribosomal protein L50, mitochondria / Ribosomal protein L27/L41, mitochondrial / Mitochondrial ribosomal protein L27 / Ribosomal subunit 39S

Component:

Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / 28S ribosomal protein S18c, mitochondrial isoform 1 / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Large ribosomal subunit protein mL49 / Large ribosomal subunit protein uL3m / Small ribosomal subunit protein uS7m / Mitochondrial ribosomal protein S11 / Large ribosomal subunit protein mL62 / 28S ribosomal protein S21, mitochondrial / Mitochondrial ribosomal protein L18 / Large ribosomal subunit protein mL40 / Large ribosomal subunit protein bL28m / 39S ribosomal protein L20, mitochondrial isoform 1 / Large ribosomal subunit protein bL33m / Large ribosomal subunit protein mL64 / 39S ribosomal protein L23, mitochondrial / Large ribosomal subunit protein bL34m / Ribosomal protein / Mitochondrial ribosomal protein S23 / 28S ribosomal protein S24, mitochondrial / Mitochondrial ribosomal protein S22 / Mitochondrial ribosomal protein L12 / Mitochondrial ribosomal protein L41 / Progonadoliberin / Small ribosomal subunit protein mS38 / Large ribosomal subunit protein uL4m / Large ribosomal subunit protein mL51 / Large ribosomal subunit protein mL37 / Small ribosomal subunit protein uS15m / Large ribosomal subunit protein uL14m / Mitochondrial ribosomal protein L57 / Large ribosomal subunit protein mL42 / 39S ribosomal protein L48, mitochondrial / Uncharacterized protein / Large ribosomal subunit protein uL24m / Large ribosomal subunit protein uL16m / Mitochondrial ribosomal protein S17 / Large ribosomal subunit protein bL17m / Large ribosomal subunit protein uL22m / Large ribosomal subunit protein uL14m / Mitochondrial ribosomal protein S18A / Mitochondrial ribosomal protein L2 / 39S ribosomal protein L15, mitochondrial / 39S ribosomal protein L27, mitochondrial / Large ribosomal subunit protein uL11m / Small ribosomal subunit protein uS10m / Large ribosomal subunit protein mL38 / Large ribosomal subunit protein uL10m / Large ribosomal subunit protein bL21m / 28S ribosomal protein S2, mitochondrial / Mitochondrial ribosomal protein L4 / Small ribosomal subunit protein mS33 / Large ribosomal subunit protein mL43 / Large ribosomal subunit protein mL52 / 28S ribosomal protein S35, mitochondrial isoform 1 / Large ribosomal subunit protein uL29m / Mitochondrial ribosomal protein L39 / Large ribosomal subunit protein mL44 / Large ribosomal subunit protein mL46 / Large ribosomal subunit protein uL30m / Coiled-coil-helix-coiled-coil-helix domain containing 1 / Small ribosomal subunit protein uS5m / 39S ribosomal protein L48, mitochondrial / Large ribosomal subunit protein bL35m / Large ribosomal subunit protein mL50 / Mitochondrial ribosomal protein L55 / Mitochondrial ribosomal protein S6 / Large ribosomal subunit protein mL45 / Large ribosomal subunit protein mL54 / Large ribosomal subunit protein bL19m / Large ribosomal subunit protein bL9m / Small ribosomal subunit protein mS29 / Large ribosomal subunit protein bL32m / 28S ribosomal protein S9, mitochondrial / Small ribosomal subunit protein mS39 / 39S ribosomal protein L13, mitochondrial / Translation initiation factor IF-2, mitochondrial / Small ribosomal subunit protein mS40

• Biological species: sus sucorfa (pig) / Pig (pig) / Homo sapiens (human) / pig (pig) / Sus scrofa (pig)
• Method: single particle reconstruction / cryo EM / Resolution: 3.2 Å
• Authors: Kummer E / Leibundgut M / Boehringer D / Ban N

Funding support

Switzerland, 3 items

Organization	Grant number	Country
European Molecular Biology Organization	ALTF 1196-2014	Switzerland
Swiss National Science Foundation	138262	Switzerland
Swiss National Science Foundation	310030B_163478	Switzerland

• Citation: Journal: Nature / Year: 2018; Title: Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM.; Authors: Eva Kummer / Marc Leibundgut / Oliver Rackham / Richard G Lee / Daniel Boehringer / Aleksandra Filipovska / Nenad Ban / ; Abstract: Mitochondria maintain their own specialized protein synthesis machinery, which in mammals is used exclusively for the synthesis of the membrane proteins responsible for oxidative phosphorylation. The initiation of protein synthesis in mitochondria differs substantially from bacterial or cytosolic translation systems. Mitochondrial translation initiation lacks initiation factor 1, which is essential in all other translation systems from bacteria to mammals. Furthermore, only one type of methionyl transfer RNA (tRNA) is used for both initiation and elongation, necessitating that the initiation factor specifically recognizes the formylated version of tRNA (fMet-tRNA). Lastly, most mitochondrial mRNAs do not possess 5' leader sequences to promote mRNA binding to the ribosome. There is currently little mechanistic insight into mammalian mitochondrial translation initiation, and it is not clear how mRNA engagement, initiator-tRNA recruitment and start-codon selection occur. Here we determine the cryo-EM structure of the complete translation initiation complex from mammalian mitochondria at 3.2 Å. We describe the function of an additional domain insertion that is present in the mammalian mitochondrial initiation factor 2 (mtIF2). By closing the decoding centre, this insertion stabilizes the binding of leaderless mRNAs and induces conformational changes in the rRNA nucleotides involved in decoding. We identify unique features of mtIF2 that are required for specific recognition of fMet-tRNA and regulation of its GTPase activity. Finally, we observe that the ribosomal tunnel in the initiating ribosome is blocked by insertion of the N-terminal portion of mitochondrial protein mL45, which becomes exposed as the ribosome switches to elongation mode and may have an additional role in targeting of mitochondrial ribosomes to the protein-conducting pore in the inner mitochondrial membrane.

History

Deposition	Apr 13, 2018	-
Header (metadata) release	Aug 15, 2018	-
Map release	Aug 22, 2018	-
Update	Dec 11, 2019	-
Current status	Dec 11, 2019	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_4368.map.gz / Format: CCP4 / Size: 84.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.39 Å

Density

Contour Level	By AUTHOR: 0.13 / Movie #1: 0.13
Minimum - Maximum	-0.7428096 - 1.4530257
Average (Standard dev.)	0.001873142 (±0.04935889)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 281 281 281 Spacing 281 281 281
Cell	A=B=C: 390.59 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.39	1.39	1.39
M x/y/z	281	281	281
origin x/y/z	0.000	0.000	0.000
length x/y/z	390.590	390.590	390.590
α/β/γ	90.000	90.000	90.000
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	281	281	281
D min/max/mean	-0.743	1.453	0.002

Supplemental data

Mask #1

• File: emd_4368_msk_1.map

Half map: #1

• File: emd_4368_half_map_1.map

Half map: #2

• File: emd_4368_half_map_2.map

Sample components

Entire : mammalian mitochondrial translation initiation complex

• Entire: Name: mammalian mitochondrial translation initiation complex

Components

• Complex: mammalian mitochondrial translation initiation complex
  • Protein or peptide: Mitochondrial ribosomal protein L12
  • Protein or peptide: Mitochondrial ribosomal protein L27
  • Protein or peptide: Mitochondrial ribosomal protein L28
  • Protein or peptide: Mitochondrial ribosomal protein L47
  • Protein or peptide: uL30m
  • Protein or peptide: 39S ribosomal protein L55, mitochondrial
  • Protein or peptide: bL32m
  • Protein or peptide: bL33m
  • Protein or peptide: Mitochondrial ribosomal protein L34
  • Protein or peptide: Mitochondrial ribosomal protein L35
  • Protein or peptide: Ribosomal protein
  • RNA: 16S ribosomal RNA, mitochondrial
  • RNA: tRNA-Phe, mitochondrial
  • Protein or peptide: Translation initiation factor IF-2, mitochondrial
  • Protein or peptide: 39S ribosomal protein L2, mitochondrial
  • Protein or peptide: ICT1
  • Protein or peptide: Mitochondrial ribosomal protein L4
  • Protein or peptide: Mitochondrial ribosomal protein L9
  • Protein or peptide: Mitochondrial ribosomal protein L10
  • Protein or peptide: Mitochondrial ribosomal protein L11
  • Protein or peptide: 39S ribosomal protein L13, mitochondrial
  • Protein or peptide: uL14m
  • Protein or peptide: 39S ribosomal protein L15, mitochondrial
  • Protein or peptide: 39S ribosomal protein L16, mitochondrial
  • Protein or peptide: 39S ribosomal protein L17, mitochondrial
  • Protein or peptide: Mitochondrial ribosomal protein L18
  • Protein or peptide: Mitochondrial ribosomal protein L19
  • Protein or peptide: Mitochondrial ribosomal protein L20
  • Protein or peptide: Mitochondrial ribosomal protein L21
  • Protein or peptide: uL22m
  • Protein or peptide: uL23m
  • Protein or peptide: 39S ribosomal protein L24, mitochondrial
  • Protein or peptide: Mitochondrial ribosomal protein L37
  • Protein or peptide: Mitochondrial ribosomal protein L38
  • Protein or peptide: Mitochondrial ribosomal protein L39
  • Protein or peptide: 39S ribosomal protein L40, mitochondrial isoform 1
  • Protein or peptide: Mitochondrial ribosomal protein L41
  • Protein or peptide: mL42
  • Protein or peptide: mL43
  • Protein or peptide: 39S ribosomal protein L44, mitochondrial
  • Protein or peptide: mL45
  • Protein or peptide: Mitochondrial ribosomal protein L46
  • Protein or peptide: Mitochondrial ribosomal protein L48
  • Protein or peptide: 39S ribosomal protein L49, mitochondrial
  • Protein or peptide: mL50
  • Protein or peptide: Mitochondrial ribosomal protein L51
  • Protein or peptide: 39S ribosomal protein L52, mitochondrial
  • Protein or peptide: mL53
  • Protein or peptide: mL54
  • Protein or peptide: Mitochondrial ribosomal protein L57
  • Protein or peptide: Peptidyl-tRNA hydrolase ICT1, mitochondrial
  • Protein or peptide: mL64
  • Protein or peptide: mL65
  • Protein or peptide: Mitochondrial ribosomal protein S18A
  • Protein or peptide: unassigned secondary structure elements
  • RNA: 12S ribosomal RNA, mitochondrial
  • Protein or peptide: Mitochondrial ribosomal protein S2
  • Protein or peptide: Mitochondrial ribosomal protein S24
  • Protein or peptide: Mitochondrial ribosomal protein S5
  • Protein or peptide: Mitochondrial ribosomal protein S6
  • Protein or peptide: Mitochondrial ribosomal protein S7
  • Protein or peptide: 28S ribosomal protein S9, mitochondrial
  • Protein or peptide: Mitochondrial ribosomal protein S10
  • Protein or peptide: 28S ribosomal protein S11, mitochondrial
  • Protein or peptide: Mitochondrial ribosomal protein S12
  • Protein or peptide: Mitochondrial ribosomal protein S14
  • Protein or peptide: Uncharacterized protein
  • Protein or peptide: bS16m
  • Protein or peptide: uS17m
  • Protein or peptide: Mitochondrial ribosomal protein S18C
  • Protein or peptide: bS21m
  • RNA: P-site fMet-tRNAMet, mitochondrial
  • RNA: MT-CO3 mRNA, mitochondrial
  • Protein or peptide: unassigned secondary structure elements
  • Protein or peptide: Mitochondrial ribosomal protein S22
  • Protein or peptide: 28S ribosomal protein S23, mitochondrial
  • Protein or peptide: Mitochondrial ribosomal protein S25
  • Protein or peptide: Mitochondrial ribosomal protein S26
  • Protein or peptide: mS27
  • Protein or peptide: 28S ribosomal protein S28, mitochondrial
  • Protein or peptide: Death associated protein 3
  • Protein or peptide: mS31
  • Protein or peptide: mS33
  • Protein or peptide: 28S ribosomal protein S34, mitochondrial
  • Protein or peptide: 28S ribosomal protein S35, mitochondrial
  • Protein or peptide: Coiled-coil-helix-coiled-coil-helix domain-containing protein 1
  • Protein or peptide: Aurora kinase A interacting protein 1
  • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
  • Protein or peptide: 28S ribosomal protein S18b, mitochondrial
• Ligand: MAGNESIUM ION
• Ligand: ZINC ION
• Ligand: GUANOSINE-5'-MONOPHOSPHATE
• Ligand: SPERMINE
• Ligand: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
• Ligand: SODIUM ION
• Ligand: N-FORMYLMETHIONINE
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: water

Supramolecule #1: mammalian mitochondrial translation initiation complex

• Supramolecule: Name: mammalian mitochondrial translation initiation complex; type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#89
• Source (natural): Organism: sus sucorfa (pig)
• Molecular weight: Theoretical: 2.85 MDa

Macromolecule #1: Mitochondrial ribosomal protein L12

• Macromolecule: Name: Mitochondrial ribosomal protein L12 / type: protein_or_peptide / ID: 1 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 7 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 21.268975 KDa

Sequence

String:

MLPAAASSLW GPCFGLRAAA LRVARHQGPR LCGVRLMRCS SHRKGEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKT LKIQDVGFMP MGAVAPGAPP AAAAPEAAEE DLPKRKEQTH FTVRLTEAKP VDKVKLIKEI KSHIQGINLV Q AKKLVESL PQEIKANVPK AEAEKIKAAL EAVGGTVVLE

Macromolecule #2: Mitochondrial ribosomal protein L27

• Macromolecule: Name: Mitochondrial ribosomal protein L27 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.943688 KDa

Sequence

String:

MALAVLALRT RAAVTALLSP PQAAALAVRY ASKKTGGSSK NLGGKSPGKR FGIKKMEGHY VHAGNILATQ RHFRWHPGAH VGLGKNKCL YALEEGVVRY TKEVYVPNPS NSEAVDLVTR LPQGAVLYKT FVHVVPAKPE GTFKLVAML

Macromolecule #3: Mitochondrial ribosomal protein L28

• Macromolecule: Name: Mitochondrial ribosomal protein L28 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 30.148953 KDa

Sequence

String:

MPLHKVPVGL WKQLRLWEGI YSRLPRHYLR SLEEARTPTP VHYRPHGAKF KINPKNWQRE RVEDVPIPVH YPPESQLGLW GGEGWVLGH RYVNNDKLSK RVRKVWKPQL FQRELYSEIL DKRFTVTVTM RTLDLIDQAC GFDFYILKTP KEDLCSKFGM D LKRGMLLR LARQDPQLHP DDPARRAAIY DRYKAFVIPE AEAEWVGLTL DEAVEKQRLL EEKDPIPLFK IFVEELLGQL QQ QALSEPA VVQTRASRK

Macromolecule #4: Mitochondrial ribosomal protein L47

• Macromolecule: Name: Mitochondrial ribosomal protein L47 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 30.085088 KDa

Sequence

String:

MAAAGLAVFC RRVSAALKAC RLLIRPQAPP STSCRFSPSL LPKNTPNVTS FHQFRIFHTT FSRRGLEEFF DDPKNWGEEK VKSGASWTC QQLRNKSNED LHKLWYVLLK ERNMLLTLEQ EAKRQRLPMP SPERLEKVVD SMDALDKVVQ EREDALRLLQ T GQEKARPG AWRRDIFGRI IWHKFKQWPI PWYLNKKYNR KRFFAMPYVE RFVRMRIEKQ ARIKARKRSL ERKKEKFLQE KF PHLSETQ KSSHV

Macromolecule #5: uL30m

• Macromolecule: Name: uL30m / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 18.58584 KDa

Sequence

String:

MAGILRSVVQ RPPGRLQTVT KGMESLICTD WIRHKFTRSR IPDKVFQPSP EDHEKYGGDP QYPHKLHIVT RIKSTKRRPY WEKDIIKML GLEKAHTPQV HKNIPSVNAK LKVVKHLIRI KPLKLPQGLP TEEDMANTCL KSNGELVVRW LLNPANQEAR K S

Macromolecule #6: 39S ribosomal protein L55, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L55, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.156537 KDa

Sequence

String:

MAAKGSLLRL LWQRVVTGAA PESCRHLYTS SWRADCNRAL LTRLHRQTYA RLYPVLLVKQ DGSTIHIRYR EPRRMLTMPV DLDSLSPEE RRARFRKREA KFKEKKEEPE LSDDFDVEQY KRFWTKK

Macromolecule #7: bL32m

• Macromolecule: Name: bL32m / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 21.53793 KDa

Sequence

String:

MASAMLVLVV PPWPAARGLL RNWWEQLQRK LQHNRLGLPL HPWGPALAVQ GPAICTEPAN DTNGSKAISS LLDSVFWMAA PKNRRSIEV NRCRRRNPQK LIKVKNNIDV CPECGHLKQK HILCGYCYEK VRKETAEIRR QMGKQEGGPF RAPTTETVVL Y SGETPSEQ DQGKRIIERE RKRPSWFTQN

Macromolecule #8: bL33m

• Macromolecule: Name: bL33m / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 7.508097 KDa

Sequence

String:

MFLSAVTFAK SKSKTILVKM MSQAGTGFSF NTKRSRLREK LTLLHYDPVV KKKVLFVEQK KIRSL

Macromolecule #9: Mitochondrial ribosomal protein L34

• Macromolecule: Name: Mitochondrial ribosomal protein L34 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 10.871729 KDa

Sequence

String:

MAFLARSVGR LLDPVSRSAA LVGGRWLQPQ AWLGFPDTWG LPAMQQTRGK ARGNEYQPSN IKRKHKHGWV RRLRTPTGVQ VILRRMHKG RKSLSH

Macromolecule #10: Mitochondrial ribosomal protein L35

• Macromolecule: Name: Mitochondrial ribosomal protein L35 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 21.548543 KDa

Sequence

String:

MAASAFAGAV RAATGILRPL HILASSAYQN CAKNACLSSV LSSRHFSHIQ TSALLSAPRL ITSVRNLMCG PTAPVLNRVA PLLPHVLKP PVRTVTYFSS RKGKRKTVKA VIYRFLRLHS GLWLRRKAGY KKKLWKKTAA RKRRLREFVF CNKTQSKLLD K MTTSFWKR RNWYADDPYQ KYQDRTNLKV

Macromolecule #11: Ribosomal protein

• Macromolecule: Name: Ribosomal protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 10.841016 KDa

Sequence

String:

MATAFLRTVL SAVGPLLHLG GRPLSTFAAG PPRAALAVGA QPSPAAALLS ARPLLGPQPA LGFKTKGVLK KRCRDCYLVK RRGRWFIYC KTNPKHKQRQ M

Macromolecule #14: Translation initiation factor IF-2, mitochondrial

• Macromolecule: Name: Translation initiation factor IF-2, mitochondrial / type: protein_or_peptide / ID: 14 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 72.321781 KDa
• Recombinant expression: Organism: Escherichia coli (E. coli)

Sequence

String:

WKSQLSSTKS KKVVEVWIGM TIEELARAME KNTDYVYEAL LNTDIDIDSL EADSHLDEVW IKEVITKAGM KLKWSKLKQD KVRKNKDAV RRPQADPALL TPRSPVVTIM GHVDHGKTTL LDKFRKTQVA AVETGGITQH IGAFLVSLPS GEKITFLDTP G HAAFSAMR ARGAQVTDIV VLVVAADDGV MKQTVESIQH AKDAQVPIIL AVNKCDKAEA DPEKVKKELL AYDVVCEDYG GD VQAVPVS ALTGDNLMAL AEATVALAEM LELKADPNGP VEGTVIESFT DKGRGLVTTA IIQRGTLRKG SVLVAGKCWA KVR LMFDEN GKTIDEAYPS MPVGITGWRD LPSAGEEILE VESEPRAREV VDWRKYEQEQ EKGQEDLKII EEKRKEHKEA HQKA REKYG HLLWKKRSIL RFLERKEQIP LKPKEKRERD SNVLSVIIKG DVDGSVEAIL NIIDTYDASH ECELELVHFG VGDVS ANDV NLAETFDGVI YGFNVNAGNV IQQSAAKKGV KIKLHKIIYR LVEDLQEELS SRLPCAVEEH PVGEASILAT FSVTEG KKK VPVAGCRVQK GQLEKQKKFK LTRNGHVIWK GSLTSLKHHK DDISIVKTGM DCGLSLDEDN MEFQVGDRIV CYEEKQI QA KTSWDPGF

Macromolecule #15: 39S ribosomal protein L2, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L2, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 33.270934 KDa

Sequence

String:

MALRVLTRAL SSLSLTPRIA VAPGLNLLPA VQVTNNVLLT LPSGLMSLPC RPILTSVALS ATSVSWKSRT KYTVMPVKMR KSGGRNHTG QIQVHGIGGG HKQRYRMIDF LRFRPEHESK PGPFEEKVIA VRYDPCRSAD IALVAGGNRK RWIIATENMK A GDTVLNSD HIGRMAVAAR EGDAHPLGAL PVGTLINNVE SEPGRGAQYI RAAGTCGVLL RKVNGTAIIQ LPSKRQMQVL ET CIATVGR VSNVDHNKRV IGKAGRNRWL GKRPNSGLWQ RKGGWAGRKI RPLPPMKSYV KLPSAAAQS

Macromolecule #16: ICT1

• Macromolecule: Name: ICT1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 38.427199 KDa

Sequence

String:

MPGWGLLGRA GARVLGCGAD GLGASRGLGN RTDICLLVRS LHGKSVTWWD EHLSEENVPF VKQLVSDENK AQLASKLCPL KDEPWPIHP WEPGSSRVGL IALKLGMMPL WTKDGQKHVV TLLQVQDCHV LKYTPKENHN GRMAALTVGG KTVSHFHKSA S ILEFYQEL GLPPKQKVKI FNVTENAVIK PGTPLYAAHF RPGQYVDVTA KTIGKGFQGV MRRWGFKGQP ATHGQTKTHR RP GAISTGD VARVWPGTKM PGQLGNIDRT AFGLKVWRIN TKHNIIYVNG SVPGHKNCLV KIKDSKLPAY KDFCKNLPFP TYF PDGDEE ALPEDLYDEN VCQPGAPSIT FT

Macromolecule #17: Mitochondrial ribosomal protein L4

• Macromolecule: Name: Mitochondrial ribosomal protein L4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 33.251469 KDa

Sequence

String:

MLQLVRAGAR TWFRPSGCRG LNTLAEEAVQ QAEKPESVAS LGLQPPVLRK CELPVPAHRR PVQAWIESLR GYEQERVGLT ELHPDVFST APRLDILHQV AIWQKNFKRI SYAKTKTRAE VRGGGRKPWV QKGSGRARHG SIRSPIWRGG GVAHGPRGPT S YYYMLPMK VRVQGLKVAL TVKLAQDDLH IVDSLELPTA DPQYLIELAR YRRWGDSVLL VDLEHEDMPQ NVVAATSGLK TF NLVPAVG LNVHSMLKHQ TLVLTLPTVA FLEEKLLWHN SRYTPLYPFR LPYCDFP

Macromolecule #18: Mitochondrial ribosomal protein L9

• Macromolecule: Name: Mitochondrial ribosomal protein L9 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 30.373449 KDa

Sequence

String:

MAAAACTAAG GGLLRAGLER LLRGGVRALL RPRLEGVTPR SERAFSLSLS RSTVIVERWW KVPLAGEGRK PRLHRRHRVY KLVEDTKHR PKDNLELVLT QSVEELGVRG DLVSVKKSVG RNRLLPQGLA VYASPENKKL FEEEKLLRQE GKLEKLQTKA G EATVKFLR RCHLEVGMKN NVKWELNPEI VARHFLRNLG VVVAPHALKL PEEPITQRGE YWCEVTVNGL DTVRVPMSVV NF ERPKTKR YKYWLAQQAA KGDVPTSSQM I

Macromolecule #19: Mitochondrial ribosomal protein L10

• Macromolecule: Name: Mitochondrial ribosomal protein L10 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 29.449689 KDa

Sequence

String:

MAAVVAGMLR GGLLPQAGRL PTFQTVRYGS KAVTRHRRVM HFERQKLMAV TEYIPPKPTI NPRCLPPPPT PPQEETGLVR LLRREIAAV FRDNRMIAVC QHVALSAEDK LLLRHQLRKH KILMKIFPNQ VLKPFLEESK YQNLLPLFVG HNLLLVSEEP K VKEMVRIL KGIPFLPLLG GCIDDTILSR QGFINYSKLP SLALVQGELV GGLTFLTAQT YSMLQHQPRQ LTALLDQYVK QQ QEGHPAV PASGQPEPPD PVPDP

Macromolecule #20: Mitochondrial ribosomal protein L11

• Macromolecule: Name: Mitochondrial ribosomal protein L11 / type: protein_or_peptide / ID: 20 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: pig (pig) / Organ: liver
• Molecular weight: Theoretical: 20.809271 KDa

Sequence

String:

MSKLSRATRA LKKPEASGMI RAIVRAGQAR PGPPLGPILG QRGVSINQFC KEFNEKTKDI KEGIPLPTKI FVKPDRTFEI KIGQPTVSY FLKAAAGIEK GARHTGKEVA GLVTLKHVYE IARVKAQDDA FALQDVPLSS VVRSIIGSAR SLGIRVVKDL S SEELAAFQ KERALFLAAQ READLAAQAE AAKK

Macromolecule #21: 39S ribosomal protein L13, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L13, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 20.633801 KDa

Sequence

String:

MSSFSKAPQQ WATFARVWYL LDGKMQPPGK LAAMASVKLQ GLHKPVYHQL SDCGDHVVIM NTRHIAFSGN KWEQKVYSSH TGYPGGFRQ VTAAQLHQKD PVAIVKLAIY GMLPKNLHRR TMMQRLHLFP DEDIPEDILK NLVEELPQPR KVPRRLDEYT Q EEIEAFPR VWSPPEDYRL

Macromolecule #22: uL14m

• Macromolecule: Name: uL14m / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.955632 KDa

Sequence

String:

MAFCTGLRGP FAQVSRALSQ RCFSTTGSLS AIQKMTRVRV VDNSALGTTP YHRPPRCIHV YNKTGVGKVG DRILLAIRGQ KKKALIVGH RMPGPRMTPR FDSNNVVLLE DNGNPVGTRI KTPIPSSLRQ REGEFSKVLA IAQNFV

Macromolecule #23: 39S ribosomal protein L15, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L15, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 33.481723 KDa

Sequence

String:

MGGPVQGGGP RALDLLRALP RVSLANLKPN PGSRKPERRP RGRRRGRKCG RGHKGERQRG TRPRLGFEGG QTPFYLRIPK YGFNEGHSF RHQYQPLSLN RLQYLIDLGR VDPTQPIDLT QLVNGRGVTI QPSKRDYGVQ LVEEGADTFK AKVNIEVQMA S ELAIAAIE KNGGVVTTAF YDPRSLEILC KPVPFFLRGQ PIPKRMLPPE ALVPYYTDAK NRGYLADPAR FPEARLELAR KY GYVLPDI TKDELFKMLS TRKDPRQIFF GLAPGWVVNM ADKKILKPTD ENLLKYYSS

Macromolecule #24: 39S ribosomal protein L16, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L16, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 28.680531 KDa

Sequence

String:

MWRLLARACA PLLRAPLSDS WAAPSATAGF KTLLPVPTFE DVAIPEKPKL RFVERVPLVP KVRRERKNLS DIRGPSTEAT EFTEGRFAI LALGGGYLHW GHFEMMRLTI NRSMDPKNMF ALWRVPAPFK PITRKGMGQR MGGGKGAIDH YVTPVKAGRL I VEMGGRCE FKEVQGFLDL VAHKLPFPAK AVSRETLEKM RKDQEERERN NQNPWTFERI ATANMLGIRK VLSPYDLTHK GR YWGKFYT PERV

Macromolecule #25: 39S ribosomal protein L17, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L17, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 19.268178 KDa

Sequence

String:

MRLSVAAAIS HGRVFRRLGL GPESRIHLLQ NLLTGLVRHE RIEASWARVD ELRGYAEKLI DYGKLGDTNE RAMRMADFWL TEKDLIPKL FQVLAPRFQG QNGGYTRMLQ IPNRNEQDRA KMAVIEYKGN CLPPLPLPRR DSNLTLLNQL LQGLRQDQEA S SHTAQTPA V

Macromolecule #26: Mitochondrial ribosomal protein L18

• Macromolecule: Name: Mitochondrial ribosomal protein L18 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 20.582568 KDa

Sequence

String:

MALRSRFWAL LSICRNPGCR AAALSTSCKP ATNPETDPVE NEAVAPEFTN RNPRNLELLA VARKERGWGT VWPSREFWHR LRVIRTQHH IEALVEHRNG QVVVSASTRE WAIKKHLYST RNVVACESVG RVLAERCLEA GINFMVYHPT PWEAASDSIK R LQHAMTEG GVVLREPRRI YE

Macromolecule #27: Mitochondrial ribosomal protein L19

• Macromolecule: Name: Mitochondrial ribosomal protein L19 / type: protein_or_peptide / ID: 27 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 33.441539 KDa

Sequence

String:

MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN PRIQEIQVVK LEKRLDNSLL YLRDALPEYS TFDVNMKPIT QEFNQEVPVN QLKVKMKPKP WSKRWERPKF NI KGIRFDL CLTEEQMREA QKWSQPWLEF DMMREYDTSK IEAAIWDEIE ASKNS

Macromolecule #28: Mitochondrial ribosomal protein L20

• Macromolecule: Name: Mitochondrial ribosomal protein L20 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 17.554793 KDa

Sequence

String:

MVFLTVPLWL RSRVTDRYWR VQEVLKHARH FRGRKNRCYR LAVRAVTRAF VKCTRARRLK KRSLRTLWIN RITAASQEHG LKYPAFIIN LIKCQVELNR KVLADLAIYE PKTFKSLAAL AKRRREEGFA AALGDGKEPD GIFSRVVQHR

Macromolecule #29: Mitochondrial ribosomal protein L21

• Macromolecule: Name: Mitochondrial ribosomal protein L21 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 23.154861 KDa

Sequence

String:

MAAAVAASVL QGTFGRLVSA YSRSVLRTSR PGTAPPWSAP RRLSSQHTSS LRGCVPKTSL GSPPWPDIVL PDPVEETRHH AEVVGQVNE LIAAGRYGRL FAVVHFAGHQ WKVTAEDLIL IENELDVACG ERIRLEKVLL VGADNFTLLG KPLLGKELVR V EATVIEKT ESWPKINMKF QKRKNYQRKR ITVNPQTVLR INSIEIAPCL C

Macromolecule #30: uL22m

• Macromolecule: Name: uL22m / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 24.313357 KDa

Sequence

String:

MAAAVLERLG GLWMQNLRGK LALRLLSSHS ILPQSHIHTS ASVEISRKWE KKNKIVYPPQ LPGEPRRPAE IYHCRRQIKY SKDKMWYLA KLIRGMSIDQ ALAQLEFSDK KGAQIIKEVL LEAQDMAVRD HNVEFRSNLY VAESTSGRGQ YLKRIRYHGR G RFGIMEKV FCHYFVKLVE GPPPPREAPK TAVAHAREYI QELRNRTITH AL

Macromolecule #31: uL23m

• Macromolecule: Name: uL23m / type: protein_or_peptide / ID: 31 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 17.643205 KDa

Sequence

String:

MARNVLYPLY QLGNPQLRVF RTNFFIQLVR PGTAQPEDTV QFRIPMEMTR VDLRNYLERI YNVPVAAVRT RVQYGSNRRR DHRNIRIKK PDYKVAYVQL ALGQTFTFPD LFPERKGASV DVDVRDQVLE DQRQKHSPDP RRGGVPGWFG L

Macromolecule #32: 39S ribosomal protein L24, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L24, mitochondrial / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 24.893529 KDa

Sequence

String:

MRLSALLALA SKVTLPPNYR YGMSRPGSLS DKRKNPPGTR RRRVPVEPIS DEDWHLFCGD KVEILEGKDA GKQGKVVQVI RQRNWVVVE GLNTHYRYVG KTVDYRGTMI PSEAPLLHNQ VKLVDPTDRK PTDVEWRFTE AGERVRVSTR SGRIIPKPEF P RADGIVPE TWIDGPKDTS VEDALEKTYV PRLKTLEEEV MEAMGIQETR RHKKVYWY

Macromolecule #33: Mitochondrial ribosomal protein L37

• Macromolecule: Name: Mitochondrial ribosomal protein L37 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 47.917988 KDa

Sequence

String:

MALASGPARR ALARLGRLGL GGCGAPRRGA YEWGVRSTRK PEPPPLDRVY EIPGLEPITY AGKMHFMPGL ARPVFPPWDP GWTHPKFRR LPPLHEHPLY KDQACYVFHQ RCRLLEGVKQ ALWLTKTQLI EGLPEKVLRL ADDPRNHIEN QDERVLNAIS H ARLWHSTE DIPKRETYCP VIVDSLIQLC KSQILKHPSL ARRICAQNNT LSATWNRESI LLQVHGSSGA RLNAKDPLPP VA SQEEVEA TKNHVLETFY PISPTMGLQE CNVYDVNDDT GFQEGYPYPC PHTLYFLESA NLRPRRFQPD QLRAKMILFA FGS ALAQAR LLYGNDSKVL EQPVVVQSVG TDGRLFQFLV LQLNTTDLAS DEGVKNLAWV DSDQLLYQHF WCLPVIKKKV VVEP VGPIG FQPETFRKFL ALYLHGAV

Macromolecule #34: Mitochondrial ribosomal protein L38

• Macromolecule: Name: Mitochondrial ribosomal protein L38 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 44.656387 KDa

Sequence

String:

MAAPWWRAAL CASRRWRGFS TSAALSRRAA PLGPMPNEDI DVSDLERLKK YRSFDRYRRR AEQEARKPHW WRTYREHFGE ESGPKDRVD IGLPPPKVSR TQQLLERKQA LRELRANVEE ERAARLQTAR IPLEAVRAEW ERTCGPYHKQ RLAEYCGLYR D LFHGATFV PRVPLHVAYA VGEDDLMPVY HGNEVTPTEA AQAPEVTYEA DEGSLWTLLL TNLDGHLLEP DAEYVHWLVT NI PGNRVTE GQETCPYLPP FPARGSGFHR FAFLLFKQDK RIDFSGDTRP SPCYQLAQRT FHTFDFYKKH QDAMTPAGLA FFQ CRWDDS VTRVFHQLLD MREPVFEFVR PPPYHPKQKR FPHRQPLRYL DRYRDSHEPT YGIY

Macromolecule #35: Mitochondrial ribosomal protein L39

• Macromolecule: Name: Mitochondrial ribosomal protein L39 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 38.523422 KDa

Sequence

String:

MAMGAWGLRL WRAVPRGEAG WRLIATSPAS QLSPTELIEM QNDLFNKEKN RQLSLTPRTE KIEVKHVGKT DPGTVFVMNK NISTPYSCA MHLSEWYCRK SILALVDGQP WDMYKPLTKS CEIKFLTFKD DDPGEVNKAY WRSCAMMMGC VIERAFKDEY V VSLVRAPE VPVIAGAFCY DVVLDKRLDE WMPTKENLHS FTKDARALIY KDLPFETLEV EAKVALEIFQ HNKYKLDFIE EK ASQNPER IVKLHRFGDF IDVSEGPLIP RTSICFQYEV SAVHNLQTQS SLVRRFQGLS LPVHLRAHFT IWNKLLERSR KMV TEDKTK PTEESAST

Macromolecule #36: 39S ribosomal protein L40, mitochondrial isoform 1

• Macromolecule: Name: 39S ribosomal protein L40, mitochondrial isoform 1 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 24.075658 KDa

Sequence

String:

MAAAALGAAS RTLRPASRLV GAWPTQTRDA HERGSLFSFW GLVPMRAEPL RKKKKVDPKK DQAAKDRLKK RIRRLEKASQ ELIPIEDFI TPVKFLNKER QRPPVELPFE ESERRALLLK RWSLYKQREH EMERSAIRSL LEAQEEALQE LRLSSPELHA E ATKRDPSL FPFERQGPDY TPPISDYQPP EGRYQDITKV YTQVEFKK

Macromolecule #37: Mitochondrial ribosomal protein L41

• Macromolecule: Name: Mitochondrial ribosomal protein L41 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 15.073404 KDa

Sequence

String:

MGLLSGAARA LVRGADRMSK WTSKRGPRTF CKGRGAKGTG FHGRDGKFVQ IKEMIPELVV PELAGFKLKP YVNYRAPEGT DTPLTAKQL FLETAAPAIE KDFKAGTFDP EHLEKYGFEP TQEGKLFQLY PKNFPR

Macromolecule #38: mL42

• Macromolecule: Name: mL42 / type: protein_or_peptide / ID: 38 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 16.680178 KDa

Sequence

String:

MALAAVKWVI SSRTILKHLF PIQNSASYCV CHKSTYSSLP DDYNCKVELA LTSDGRTIVC YHPSVDIPYE HTKPIPRPDP VHNIEETHD LVLKTRLEEK GEHLEQGPMI EQLSKMFFTT KHRWYPRGQY HRRRRKPNPP KDR

Macromolecule #39: mL43

• Macromolecule: Name: mL43 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 17.728109 KDa

Sequence

String:

MTARGTASRF LTSVLHNGLG RYVQQLQRLS FSLSRDAPSS RGAREFVERE VTDFARRNPG VVIYVNPRPC CVPRVVAEYL NGAVREESI HCKSVEEIAA LVQKLADQSG LDVIRIRKPF HTDSPSIQGQ WHPFTNKPTT LGGLRPREVQ DPAPAQVQAQ

Macromolecule #40: 39S ribosomal protein L44, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L44, mitochondrial / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 37.527195 KDa

Sequence

String:

MASGLVRLLQ WGPRRLLAPA APTLAPPVRG AKKGFRAAYR FQKELERWRL LRCPPPPVRR SEKPNWDYHA EIQAFGHRLQ ETFSLDLLK TAFVNSCYIK SEEAKRQKLG IDKEAALLNL KDNQELSEQG ISFSQTCLTQ FFEDAFPDLP TEGVTSLVDF L TSEEVVCH VARNLAVEQL ALSAEFPVPP PVLRQTFFAV IGALLQSSGP ERTALFIRDF LITQMTGKEL FEMWTITNPM GL LVEELKK RKISAPESRL TRQSGSTTAL PVYFVGLYCD RKLIAEGPGE TVLVAEEEAA RVALRKLFGF TENRRPWDYS KPK EHVRAE KTITAS

Macromolecule #41: mL45

• Macromolecule: Name: mL45 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 35.331805 KDa

Sequence

String:

MAAPVTRGLS CLPRVLGWWS RQPVLVTQST AVVPVRTKKR FTPPTYQPKY KSEKEFVEHA RKAGLVIPHE RLERPIHLAC TAGIFDAYV PPEGDARISS LSKEGLAQRA ERLKKNVASQ LSIRKIRESD PNFKIKDFPE KAKDIFIEAH LCLNNSDHDR L HTLVTENC FPDMVWDIRY KTVRWSFVES LEPPQVVQVR CSSLMNQGNI YGQVTVRMHT RQTLAIYDRF GRLMYGQEDV PR DVLEYVV FEKHLVDPYG SWRMHGKIIP PWAPPKQPIL KTVMIPGPQL KPWEEFEEPQ GEVHKPQPA

Macromolecule #42: Mitochondrial ribosomal protein L46

• Macromolecule: Name: Mitochondrial ribosomal protein L46 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 32.215998 KDa

Sequence

String:

MAAPVRRTML RVVRGWRRFE GPWAHSLGSR NLALAVAPSS SSSPWRLLGA LCLQRPPLVT KPLTPLQEEM ADLLQQIEIE RSLYSDHEL RALDEAQQLA KKKSDLYEEE DEQNILLAQD LEDMWEQKFL HFKLGARLTE ADKKDDRTSL HRKLDRNLIL L VREKLGDQ DIWMLPQSDW QPGETLRQTA ERTLATLSEN NMEAKFLGNA PCGHYKFKFP QAMRTETSLG AKVFFFKALL LT GDFSQAG KKGHHVWVSK EELGDYLKPK YLAQVRRFLL DL

Macromolecule #43: Mitochondrial ribosomal protein L48

• Macromolecule: Name: Mitochondrial ribosomal protein L48 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 23.749512 KDa

Sequence

String:

MNGALGKALC LRNDTVLKQA LSLIRVRASG ESPICSAGGI LLSTSRHYRS KPTHGIGRYK HLVKAQEPKK KKGKVEVRPI NLGTDYEYG VLNIHLIAYD MALAESYAQY VHNLCNHLAI KVEESYAMPT KTMEVLQLQE QGSKMFLDAV LTTHERVVQI S GLSATFAE IFLEIIHSNL PEGVKLSVRE HTEEDFKGRF KARPELEELL AKLN

Macromolecule #44: 39S ribosomal protein L49, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L49, mitochondrial / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 18.957779 KDa

Sequence

String:

MAATVLCGVL RAWRTGVPLG CGLRRLSQTQ GTPEYPSFVE SVDEYHFVER LLPPASIPRP PKHEHYPTPS GWQPPRDPAP SLPYFVRRS RMHNIPVYRD ITHGNRQMTV IRKVEGDIWA LQKDVEDFLS PLLGKTPVTQ VNEVTGTLRV KGYFDQQLKA W LLEKGF

Macromolecule #45: mL50

• Macromolecule: Name: mL50 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 18.351 KDa

Sequence

String:

MAARWVSGLA RRSLTCAVSG APRREFWSPF RKEKQPVVAE TVEEVKKEPI LVCPPIQSRT YIPPEDLQSR LESHVKEVFG SSVPSNWQD ISLEDVHLKF SFLARLANDL RHAVPNSRLH QMCRVRDVLD FYNVPVQDRS KFDELIASNL PHNLKITWGY

Macromolecule #46: Mitochondrial ribosomal protein L51

• Macromolecule: Name: Mitochondrial ribosomal protein L51 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 15.249048 KDa

Sequence

String:

MAGSLSWVTG RGLWGRLPLT CRSFSLGIPR LFHVRVTLPP RKVVDRWNEK RAMFGVYDNI GILGNFEKHP KELIKGPIWL RGWKGNELQ RCIRKKRMVG NRMFIDDLHN LNKRISYLYK HFNRHGKYR

Macromolecule #47: 39S ribosomal protein L52, mitochondrial

• Macromolecule: Name: 39S ribosomal protein L52, mitochondrial / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 13.813705 KDa

Sequence

String:

MAAWGILLST GVRRLHCGTA AQAGSQWRLQ QGFAANPSGY GPLTELPDWS YADGRPAPPM KGQLRRKAQR EKFARRVVLL SQEMDAGLQ AWQLRQQEKL QEEEGKQKNA LKSKGALLQN PQPSQ

Macromolecule #48: mL53

• Macromolecule: Name: mL53 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 12.029772 KDa

Sequence

String:

MAAALARLGL RAVKQVRVQF CPFEKNVEST RTFLQAVSSE KVRCTNLNCS VIADVRHDGS EPCVDVLFGD GHRLIMRGAH LTAQEMLTA FASHIQARGA AASGDKPSAS TGR

Macromolecule #49: mL54

• Macromolecule: Name: mL54 / type: protein_or_peptide / ID: 49 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.957357 KDa

Sequence

String:

MAARRLFGAA RSWAAWRAWE LSDAAVSGRL HVRNYAKRPV IKGGKGGKGA VVGEALKDPE VCTDPFRLTT HAMGVNIYKE GQDVVLKPD SEYPEWLFEM NVGPPKKLEE LDPETREYWR LLRKHNIWRH NRLSKNRKF

Macromolecule #50: Mitochondrial ribosomal protein L57

• Macromolecule: Name: Mitochondrial ribosomal protein L57 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 12.001915 KDa

Sequence

String:

MFLTALLRRN RIPGRQWIGK HRRPRPVSAQ AKQNMIRRLE TEAENQYWLS RPFLTAEQER GHAAVRRAAA FQALKAAQAA RFPAHRRLE EQLGHLLVTR KWS

Macromolecule #51: Peptidyl-tRNA hydrolase ICT1, mitochondrial

• Macromolecule: Name: Peptidyl-tRNA hydrolase ICT1, mitochondrial / type: protein_or_peptide / ID: 51 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 23.545873 KDa

Sequence

String:

MAAARCLRWG LNRAGAWLLP SPTRYPRRAL HKQVEGTEFQ SIYSLDKLYP ESRGSDTAWR LPDDAKQAND IPVDRLTISY CRSSGPGGQ NVNKVNSKAE VRFHLASADW IAEPVRLKLA VKHKNRINRS GELILTSECS RYQFRNLADC LQKLRDMIAE A SQPAKEPS REDAELRRSR IENMNRERLR KKRISSAIKT SRRVDVD

Macromolecule #52: mL64

• Macromolecule: Name: mL64 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 25.815031 KDa

Sequence

String:

MAAPVQQART LLRLVTTLGQ GSRGYRARPP PRRSQEPRWP DPDDPLTPRW QLSPRYAAKQ FARHGAASGV AAGSLWPSQE QLRELEAEE REWYPSLAAM QESLRVQQLA EEQKRQAREQ LIEECMAKMP QMIENWRQQQ QERREKEQAD KDRRARLQAE A QERLGYHV DPRSARFQEL LQDMEKQHRK RLKEEKQRKK KEARAAAMAA AAAQDPADSE TPSS

Macromolecule #53: mL65

• Macromolecule: Name: mL65 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 49.100504 KDa

Sequence

String:

MAAARCWRFP LRGAGLSLHT AAKAAVTAPE VTGPDVPATP VARYPPIVAS LTAKSKAARQ RRVEQWQATV HAAKSVDEKL RILTKMQFM KYVVYPQTFA LNADNWYQSF TKTVFLSGLP PTPAKLEPEP TLDITALREA VCDCLLQEHF FLRRKKRAPV I QDREAIAS PFLDQLVASL TGLLSVHNPV LAAAALDCKR PVHFFWLRGE EIIPRGHRKG RVDALRYQIN DKPHNQIRIS RQ LPEFVPL DYSIPIEVPV MSCKPDKLPL FKRQYENTIF IGSKTADPLC YGHTQFHLLP DKLKREKLLK QNCADQIEVV FRA NAIASL FAWTGAQAMY QGFWSEADVT RPFVSQGVIT DGKYFSFFCY QLNTLALTAQ ADQNNPRKNI CWGTQSKPLY ETIE DNNVK GFNDDVLLQL VQFLLNRPKE DKSQLLEN

Macromolecule #54: Mitochondrial ribosomal protein S18A

• Macromolecule: Name: Mitochondrial ribosomal protein S18A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 22.273084 KDa

Sequence

String:

MVGLNVLVSG CGRLLRGLLA GPAATSWARP PSRGFREVVE ILDGKTTIIE GRITETPQES PNPPNPTGQC PICRWNLKHK YNYEDVLLL SQFIRPHGGM LPRRITGLCQ EEHLKIEECV KMAHRAGLLP NHRPKLPEGF VPKSKPRLNR YLTRWSPRSV K PIYNKGHR WNKVRMPVGS PLLKDNVSYS GRPLLLYH

Macromolecule #55: unassigned secondary structure elements

• Macromolecule: Name: unassigned secondary structure elements / type: protein_or_peptide / ID: 55 ; Details: Unassigned secondary structure elements built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 5.846387 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA

Macromolecule #57: Mitochondrial ribosomal protein S2

• Macromolecule: Name: Mitochondrial ribosomal protein S2 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 31.731604 KDa

Sequence

String:

MASGPLLPRL LGAGAPRRPL RLHLVPTATP GPAGPTGRTL GSNAAPAARE PELGNDLRDR ILSEPLKHAD FFNLKELFSV RSLFDARVH LGHKAGCRHR FMEPYLFGSR LGQDIIDLEQ TAAHLQLALN FTAHVAYREG IILFVSRHRQ FAHLIETTAR D CGEYAHTR YFKGGLLTNA PLLLGPGVRL PDLIIFLHTL NNVFEPHVAV RDAAKMNIPT VGIVDTNCNP ALITYPVPGN DD SPPAVRL FCRLFQVAIS RAKEKRRQVE ALYRLQGPAG AERGPAAPPP PE

Macromolecule #58: Mitochondrial ribosomal protein S24

• Macromolecule: Name: Mitochondrial ribosomal protein S24 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 18.935172 KDa

Sequence

String:

MVASVCGGGL RPLVLSWSRE LPCAWRALHI SAVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMLGT FPGCLADQLV LKRRANQLEI CALVLRQLPP HKFYFLVGYS ETLLSHFYKC PVHLHLQTVP S KVVYKYI

Macromolecule #59: Mitochondrial ribosomal protein S5

• Macromolecule: Name: Mitochondrial ribosomal protein S5 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 48.213074 KDa

Sequence

String:

MAAAVRAAGF FPGLFGASAG NLWSRQLYLN TFPAASILTS KTFPSNGPLS PPGTRDNHHF TSLACALQTQ CSISSPSNWM GQQYRSYSF FTKLTADELW KGALAESGAG ARKGRGKRTK KKRRKDLNRG QIIGEGRHGF LWPGLNIPLM RNGAVQTIAQ R SKEDQEKV EADMVQQREE WDRRRKMKVK RERGWSGNTW GGVSLGPPDP GPNGETYDDF DTRILEVRNV FNMTAKEGRK RS VRVLVAV GNGKGAAGFA IGKATERADA FRKAKNRAVH YLHYIERYED HTIYHDISLK FKRTHIKMKK QPRGYGLHCH RAI MTICRL IGIKDLYAKV SGSVNMLNLT RGLFLGLSRQ ETHQQLADKK SLHVVEFREE CGPLPIVVAS PQGALRKDPE PEDE VPDIT LDWEDVKAAQ GMKRSVWSGL KRAAT

Macromolecule #60: Mitochondrial ribosomal protein S6

• Macromolecule: Name: Mitochondrial ribosomal protein S6 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 14.283703 KDa

Sequence

String:

MPRYELALIL KAMQRPETAA ALKRTLEALM DRGAVVRNLE NLGERMLPYK ISAHNQRHSR GGYFLVDFYA PATTVESMME HLSRDIDVI RPNIVKHPLT QEVKECEGIV PVPLEEKLYS TKKRK

Macromolecule #61: Mitochondrial ribosomal protein S7

• Macromolecule: Name: Mitochondrial ribosomal protein S7 / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 28.150918 KDa

Sequence

String:

MAAPAVKAAR GWSCSALGVR IAVLRFPGLT QVRWSRYGPE YKDPQIDKEY YRKPLAEQTE EEKYERDFKK TQLIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKVLARS LMTQTLEAVK RKQFAKYHAA SAEEQATIER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY QVPVPLADRR RRFLAMKWMI AECREKKHRR VLMPEKLSQE LLEAFHNQGP VIKRKHDMHK MAEANRALAH YR WW

Macromolecule #62: 28S ribosomal protein S9, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 62 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 45.60457 KDa

Sequence

String:

MAAPCVFCGA AVSYRLFLGC RVSFARGQVL WKAAAPELQA GARCQILRLR HTAVVTTKKN VAASRRETYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPIMKHPE EIFPKQRAIQ WGEDGRPFHF LFYTGKQSYY S LMHDTYGK LLDVEKHHNQ LRAKDLLAEK TKILKDPIGS RWLIKEELEE MLVEKLSDQD YAQFIRLLER LSALPCGATE ED FVNRFRR SIPIQSKKQL IEPLQYDEQG MAFSRGEGKR KTAKAEVVVY GQGSGRIDVN GVDYLLYFPV TQDREQLMFP LHF LDRLGK HDMTCAVSGG GRSAQAGAVR LAMARALCSF VTEDEVEWMR QAGLLTADPR VRERKKPGQE GARRKFTWKK R

Macromolecule #63: Mitochondrial ribosomal protein S10

• Macromolecule: Name: Mitochondrial ribosomal protein S10 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 23.192758 KDa

Sequence

String:

MAARTAFGAL ARRLWQGSRN FSVSSSRSST AKNGGFLLST TMKWVQFSNL HVDVPKDMTK PEITISDEPD TLYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLKSVHIFKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV AMEVTKTKLE QLPEHIRKPI WETMPEEKEE SKS

Macromolecule #64: 28S ribosomal protein S11, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S11, mitochondrial / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 20.841922 KDa

Sequence

String:

MQVVRNAGSR LLQSWAWPPK TRIVAGLPAS TIHRSAPHLQ DAAAKLDIED KEEARTPSHS SFSIYPPIPG QENSLRWAGK KFEEIPIAH IKASYNNTQI QVVSAAHQPL AHASCGTEGF RNAKKGTGIA AQTAGIAAAA KATGKGVTHV RVVVKGLGPG R LSAIKGLT MGGLEVISIT DNTPIPHNGC RPRKARRL

Macromolecule #65: Mitochondrial ribosomal protein S12

• Macromolecule: Name: Mitochondrial ribosomal protein S12 / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.257107 KDa

Sequence

String:

MSWSGLLRGL GTSLNCGLAL VPRPWATRPM ATLNQMHRLG PPKHPPGKMG PTAGRPQLKG VVLRTFIRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HSLQEHHVVL VQGGRTQDLP GVKLTVVRGK YDCGHVQKKK

Macromolecule #66: Mitochondrial ribosomal protein S14

• Macromolecule: Name: Mitochondrial ribosomal protein S14 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 15.120739 KDa

Sequence

String:

MAASMLGFLL RTVRQMVPSS ASGQVRSYYV DWKMLRDVKR RKMAYEYADE RLRINSLRKN TILPKDLQEV ADEEIASLPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGVQRAMW

Macromolecule #67: Uncharacterized protein

• Macromolecule: Name: Uncharacterized protein / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 27.411428 KDa

Sequence

String:

MLRAAWRALS SIRTQAVTQA PVLGLPGGGC ARFPSVQRAL PSRPAGLILR AARGYAAQKP VQPNQDDPPP SMLLLDYQNV PGIHKVDDV VKRLLSLEMA NQKEKLKIKK MQLMNKVLEN PEDTSSLEAR IVALTVKIRN YEEHMQKHRK DKAHKRFLLM S IDQRKKML KNLRETNYAV FEKICKELGI EYTFPPPYHR KAHRRWVTKK ALCTQVFREV QKLKKQKRAL RAAAVAAHKQ G

Macromolecule #68: bS16m

• Macromolecule: Name: bS16m / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 15.182668 KDa

Sequence

String:

MVQLTTVLCR AYRGGHLTIR LALGGCTNRP FYRIVAAHSK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNF DRIRHWIGCG AHLSKPVEK LLGLSGFYPL HPMMITNAER LRRKRAREVL LASQKTDTEA TETKAS

Macromolecule #69: uS17m

• Macromolecule: Name: uS17m / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 14.452014 KDa

Sequence

String:

MSIARSSVHA KWVVGKVIGT AMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLKALP VPRTKHVKHE LAEIIFKVG QVIDPVTGKP CAGTTYLESP ISPESTRLTK NLEALQLSST Q

Macromolecule #70: Mitochondrial ribosomal protein S18C

• Macromolecule: Name: Mitochondrial ribosomal protein S18C / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 16.211086 KDa

Sequence

String:

MAAVVSLCSR LGRRKSTDFV NAAVCLTDPG THAVLWRSCS QYKQVASNED LPVPMENPYK EPLKKCILCE KHVDYKNVQL LSQFISPFT GCIYGRHITG LCGKKQKEIT KAIKRAQILG FMPVTYKDPA YLKDPKVCNI KYRE

Macromolecule #71: bS21m

• Macromolecule: Name: bS21m / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 10.682506 KDa

Sequence

String:

MANHLKFIAR TVMVQEGNVE GAYRTLNRIL TMDGLIEDIK RRRYYEKPCR RRQRESYETC RRIYNMEMAR KINFLMRKNR ADPWQGC

Macromolecule #74: unassigned secondary structure elements

• Macromolecule: Name: unassigned secondary structure elements / type: protein_or_peptide / ID: 74 ; Details: Unassigned secondary structure elements built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig) / Organ: liver
• Molecular weight: Theoretical: 1.297416 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAA

Macromolecule #75: Mitochondrial ribosomal protein S22

• Macromolecule: Name: Mitochondrial ribosomal protein S22 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 43.234645 KDa

Sequence

String:

MPHEPRETRN LSLPLPALVA RIIMATLKAS ALLRSLQTNS CGTGRVCFPV RARPRPRALL QPLPGASGTG TLCRGLGSES ESGNSEIRK PTFMDEEVQN ILIKMTGLDL QKIFKPALQE LKPPTYKLMT QAQLEEATKQ AVEAAKVRLK MPPVLEERAP I NDVLAEDK ILEGTETAKY VFTDISYSIP HRERFIVVRE PSGTLRKASW EERDRMIQVY FPREGRRILT PVIFKEENLQ TM YSQDQHV DVLNLCVAQF EPDSAEYIKI HHHTYEDIDK CGKYDLLRST RHFGGMAWYF VNKKKIDGLL IDQIQRDLVS DAT SLVHLY HILHPDGQSA QEAKKQGAEG LHLIKVFAKT EAQKGAYIEL TLQAYQEAFI THSAAS

Macromolecule #76: 28S ribosomal protein S23, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 21.666674 KDa

Sequence

String:

MAGSRLETVG SIFSRTRDLI RAGVLKEKPL WLDIYNAFPP LREPVFRRPR LRYGKAKAAV QDIFYHEDRI RAKFYSAYGS GPKAFDLFN PNFKSTCQRF VEKYIELQRL GETDEEKLFV EAGKALLAEG VTLRRVGEAR TQQEGSPISR KSEPTGIKPQ T AVEENQPW KEVPQDQPLE APEKESKGLP PP

Macromolecule #77: Mitochondrial ribosomal protein S25

• Macromolecule: Name: Mitochondrial ribosomal protein S25 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 19.954186 KDa

Sequence

String:

MPMKGRFPIR RTLQYLGQGD VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQITMFKNM TPSPFLRFYL DSGEQVLVD VESKSNKEIV EHIRKILGKN EETLEKEEQE KKQLSHPAHF GPRKYCLREC MCEVEGQVPC PGLVPLPKEM T GKYKAALK ATAQD

Macromolecule #78: Mitochondrial ribosomal protein S26

• Macromolecule: Name: Mitochondrial ribosomal protein S26 / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 23.6379 KDa

Sequence

String:

MLRVLGGLGA RPAGRLPAPL LLPTRGRKTR HDPPAKSKAG RVATPPAVDP TEFFVLTERY RQYRQTVRAL RLEFMSEVRK KLHEARAGV QAERKAQEDA AEHRELMAWN QAENQRLHEL RLARLRQEAL EQERRQAEEA VLQAREAQAW AQLKEQEVLQ L QEEAKTFI TRENLEARVE EALDSPKSYN WAITREGLVV RPQQKGS

Macromolecule #79: mS27

• Macromolecule: Name: mS27 / type: protein_or_peptide / ID: 79 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 51.945984 KDa

Sequence

String:

MLSRAAPTEG TGGGRRSVDS TPPPRLPATP APAFPLVCSK MASSIVRRGI LRARKVSLPQ LSLAGRRCLL SAAYVDSHKW EARDKEDCH LADLASLMDK TYERKLPVSS LTISRFVDNI ASREDIDHAE YYLYKFRHSP NCWYLRDWTI HTWIRQCLKY G AQDKALYT LKNKVQYGIF PDNYTFNLLM DYFIKKENYK DALSVVFEIM MQEAFEVPST QLLSLYVLYH CLAEKTDLSW EE ERNFGAS LLLPGLKQKN SVGLSSQLYG YALLGKVELQ QGLRAVYCNM PLLWQPGYLH RALQVMEKVA SSPEDGQLCG EAL DVLDRV LKAVTAPAQG TSEEQPQEGE ESQRSEELVE QLDVEETEQS KLPQYLERFE ALRSKLQALG KVESESLLTL STQL AKAQL PTCEAEDIAV YEQRLQQWHG ERVRLIQREQ EQRERARQDY EARRAAKASA

Macromolecule #80: 28S ribosomal protein S28, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 80 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 21.014912 KDa

Sequence

String:

MAALCRTRAV TAKSHFLRVF FFSRPCRGTG TESGSGSESS ESTEPKQRPG GFASALERHS ELQQKAEFGR MRGSPKNVES FASMLRHSP LTQMGPAKNK LVIGQIFHIV EDDLYIDFGG KFHCVCKRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT D TTILEADA VLLGLQESKD SKSKEERHEK

Macromolecule #81: Death associated protein 3

• Macromolecule: Name: Death associated protein 3 / type: protein_or_peptide / ID: 81 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig) / Organ: liver
• Molecular weight: Theoretical: 45.582414 KDa

Sequence

String:

MLKGVTRLIS RVHKLDPGHV LYMGTQAPQS LAAHLDNQVP VKSPRAISRT SEDDPAKHRE QHEGQHYNIS LQELKTVFPH GLPPRFAMQ VKTFNEACLM VRKPALELLH YLKNTNFAHP AVRYVLYGEK GTGKTLSLCH ILHFCAKQNW LILHIPDAHI W VKNCRDLL QSNYNKQRFD QPLEASTWLK NFKTANEHFL SQIKVQEKYV WNKRESTEKG RPLGEVVEQG IMRVRNATDA VG IVLKELK RQSSLGIFHL LVAVDGVNAL WGRTTLKRED KSPIAPEELA LIHNLRKMVK NDWQGGAIVL TVSQTGSLFK PRN AYLPQE LLGKEGFDAL DPFIPILVSN YNPKEFESCI QYYLENNWLQ HEKAHTEEGK KELLFLSNRN PGQLERLCAY L

Macromolecule #82: mS31

• Macromolecule: Name: mS31 / type: protein_or_peptide / ID: 82 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 44.091996 KDa

Sequence

String:

MLPRVSAVIP LRPLSRLPLC SGGPEASAAA ILLLDSLRGT VRTENNIQRY FGTNNLVYSK KDEQSGPTCE ISKETESKGS VKENSKKDL LNIIKGMKVE LSTVNVQTTK PSNKRQLKNL ETAIGRIKKA PEDTPQKRSE SLSSELVAAV SAVADSLPFD R RMTKSELL RQLQKHEEDS R(UNK)QKDGEKPK ISFSNIISDM KVARSATARV STRPVHQIQF DEGTDDFADQ EKTLNLKK S FRKNIFKGRR LNIFEPQAVT EEAPETEAVP SLWDIEFAKQ LAAVTERPFQ NGFEEMIQWT KEGKLWEFPI NNEAGFDDD GSEFHEHIFL DKYLQDFPKQ GPIRHFMELV TCGLSKNPYL SVKQKVEHIE WFRNYFNEKR VILKESGIQL N

Macromolecule #83: mS33

• Macromolecule: Name: mS33 / type: protein_or_peptide / ID: 83 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 12.54465 KDa

Sequence

String:

MSSLSEYAVR MSRLSARLFG EVARPTDSKS MKVVKLFSEQ PLAKRKETYD WYPNHNTYFA LMGTLRFLGL YRDEHQDFRD EQLRLKKLR GKGKPRKGEG KRAMKKK

Macromolecule #84: 28S ribosomal protein S34, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S34, mitochondrial / type: protein_or_peptide / ID: 84 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 25.843818 KDa

Sequence

String:

MARKKVRPRL IAELARRVRA LREQRERPRD SQRYALDYET MMRPHSGNRL PMRAWADVRH ESRLLQLLNR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYAAENL DHGKAWGILT FKGKTESEAR EIEQVMYHDW RLVPKHEEEA FTAFTLVPED T PLSVPYPP LLRAMILAQR QKYGDTSTEE PMLNLERARI DPWDYPAKLE AKTKTKGAAV

Macromolecule #85: 28S ribosomal protein S35, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 85 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 37.104809 KDa

Sequence

String:

MAATSLPAWL ILKSRAGTFR AFSTAMSPAT RSPRPALRTT ERTSKPERAL RRKALPPRTE KMAVDQDWPS VYPVAAPFKP SAVPLPVRM GYPVKRGVPM AKEGNLELLK IPNFLHLTPV AIKRHCEALK DFCTEWPAAL DSDEKCEKHF PIEIDTADYV S AGPSIRNP KARVVTLRVK LSSLNLDDHA KKKLIKLVGD RYCKSTDVLT IKTDRCPLKR QNYDYAVYLL TVLYHESWKT EE WEKKKTE ADMEEYIWEN STSEKNILET LLQIKAAEKN LELSKEELLG TKEVEDYRKS VVSLKNEGDN ENTLSQYKES VKR LLNLA

Macromolecule #86: Coiled-coil-helix-coiled-coil-helix domain-containing protein 1

• Macromolecule: Name: Coiled-coil-helix-coiled-coil-helix domain-containing protein 1; type: protein_or_peptide / ID: 86 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 13.561903 KDa

Sequence

String:

MATPSLRGRL ARLGNPRRPI LKPNKPLILA NHVGERRREK GEATCITEMS VMMACWKQNE FRDEACRKEI QDFFDCASRA EAARKMRSI QENLGELGSL PPKKLNKLLH RFPNKPHVS

Macromolecule #87: Aurora kinase A interacting protein 1

• Macromolecule: Name: Aurora kinase A interacting protein 1 / type: protein_or_peptide / ID: 87 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 22.843941 KDa

Sequence

String:

MFLVRLTSQL LRAVPGAGRS GSPRVSEVLG RHACRPRYST QPPGPSGIAS LPGKHVHPEL EEMLVPRKMS ISPLESWLTI RYLLPRLGA GTPGTVSPAQ LYQCPPSQVG EGVKQGDKEV RDTPQMQCKN ILKIRRRKMN HHKYRKLVKR TRFLRRKVRE G RLRQKQVK FERDLKRIWQ KAGLKEAPAG WQTPKIYLKN Q

Macromolecule #88: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

• Macromolecule: Name: Pentatricopeptide repeat domain-containing protein 3, mitochondrial; type: protein_or_peptide / ID: 88 ; Details: Unassigned residues have been built as poly-alanine.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 78.518156 KDa

Sequence

String:

MAAVASARWL GVRSGLCLPL TGRRVGPCGR TPRSRFYSGS AAHPEVEGAN VTGIEEVVIP KKKTWDKVAI LQALASTVHR DSTAAPYVF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI EDVSEAALQE R IKLRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GNQEPSANYN FQQREQSEEL EEATEADNEK SKTKAGHHLG VT WRTKNNA ERIFALMPEK NAHSYCTMIR GMVKHQAPTQ ALNLYTVLLN NRLRADVYTF NSLIEATALV VNEKFEEKWN NIL DLLKQM VTQNVKPNLQ TFNTILKCLR RFYAFGKLPA LQTLREMKAI GIEPSLATYH YVIQLFYQHE SPSKGSSLII YDIM NEVMG KRFSPRDPDD DMFFQSAMRV CSSLRDLELA YQVHGLLNTG DNWKLIGSDH RRNFYYSKFF NLLCFMEQID VTLKW YKDL IPSVFFPHSQ TMIDLLQALD VANRLDMVPQ IWKDSKEYGH TFRNELKEEI LMLMARDQHP PELQVAFADC AADIKS TYE SQDARQTAPE WPASSLNYVA VLFLRAGRTQ EAWKMLGLFR KHNKIPRAEL LNEFLDSAKA SSSPAQAIEL VKLASAF SL PVCEGLTRRV MAEFTLTQEQ REALGELTAL TSDSSSDSDS DSDSDISEGK

Macromolecule #89: 28S ribosomal protein S18b, mitochondrial

• Macromolecule: Name: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 89 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Pig (pig)
• Molecular weight: Theoretical: 29.220465 KDa

Sequence

String:

MAASILNVLL RRLPGVSPFR GAYGVQVLLQ TLCTKAPPED DSLPQVPISP YKDEPWKYLD SEEYQNRYGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLEQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLRYHIP QVEPRDLDFS TTHGAVSSTP PAPTLVSGDP WYPWYSWKQP PERELSRLRR LYQGRLREES GPPPELMPEV PL TAPAEAS STEPGAPQSA L

Macromolecule #12: 16S ribosomal RNA, mitochondrial

• Macromolecule: Name: 16S ribosomal RNA, mitochondrial / type: rna / ID: 12 / Number of copies: 1
• Source (natural): Organism: pig (pig) / Organ: liver
• Molecular weight: Theoretical: 504.852031 KDa

Sequence

String:

ACCAAAGCUA GCUCAACAUA CUAAACAAAU ACAAAAAUAC ACCAAAAUAA AAUAAAACAU UCACCUAACA UUAAAGUAUA GGAGAUAGA AAUUUUUAUC CUGACGCUAU AGAGAUAGUA CCGUAAGGGA AAGAUGAAAG AAUAAAAUAA AAGUAAAAAA A AGCAAAGA UUACCCCUUC UACCUUUUGC AUAAUGGUUU AACCAGAAAA AAUCUAACAA AGAGAACUUU AGCUAGAUAC CC CGAAACC AGACGAGCUA CCCAUGAGCA GUUUAAAAGA ACCAACUCAU CUAUGUGGCA AAAUAGUGAG AAGACUUGUA GGU AGAGGU GAAAAGCCUA ACGAGCCUGG UGAUAGCUGG UUGUCCGAGA AAGAAUUUUA GUUCAACCUU AAAAAUACCC CAAA AACCC UAAAUUCCAA UGUAUUUUUA AGAGAUAGUC UAAAAAGGUA CAGCUUUUUA GAAACGGAUA CAACCUUGAC UAGAG AGUA AAAUCUUAAU ACUACCAUAG UAGGCCUAAA AGCAGCCAUC AAUUGAGAAA GCGUUAAAGC UCAACAAAUU CACCAA CAU AAUCCCAAAA ACUAAUAACA AACUCCUAGC CCAAUACCGG ACUAAUCUAU UGAAACAUAG AAGCAAUAAU GUUAAUA UG AGUAACAAGA AGCCUUUCUC CUCGCACACG CUUACAUCAG UAACUAAUAA UAUACUGAUA AUUAACAACC AAUAAACC A AAACAACACU AAAACGUUUA UUAAUUACAU UGUUAACCCA ACACAGGAGU GCACCAAGGA AAGAUUAAAA GAAGUAAAA GGAACUCGGC AAACACAAAC CCCGCCUGUU UACCAAAAAC AUCACCUCUA GCAUUACUAG UAUUAGAGGC AAUGCCUGCC CAGUGACAC CAGUUUAACG GCCGCGGUAU UCUGACCGUG CAAAGGUAGC AUAAUCACUU GUUCUCCAAA UAAGGACUUG U AUGAAUGG CCACACGAGG GUUUUACUGU CUCUUACUUC CAAUCAGUGA AAUUAACCUU CCCGUGAAGA GGCGGGAAUA AA AAAAUAA GACGAGAAGA CCCUAUGGAG CUUUAAUUAA CUAUUCCAAA AGUUAAACAA CUCAACCACA AAGGGAUAAA ACA UAACUU AACAUGGACU AGCAAUUUCG GUUGGGGUGA CCUCGGAGUA CAAAAAACCC UCCGAGUGAU UUUAAUCUAG ACAA ACCAG UCAAAAUAAC CAUAACAUCA CUUAUUGAUC CAAAAUUUUG AUCAACGGAA CAAGUUACCC UAGGGAUAAC AGCGC AAUC CUGUUCUAGA GUUCCUAUCG ACAAUAGGGU UUACGACCUC GAUGUUGGAU CAGGACACCC AAAUGGUGCA ACCGCU AUU AAAGGUUCGU UUGUUCAACG AUUAAAGUCC UACGUGAUCU GAGUUCAGAC CGGAGCAAUC CAGGUCGGUU UCUAUCU AU UAUAAAUUUC UCCCAGUACG AAAGGACAAG AGAAAUGGGA CCAACCUCAC AAACGCGUCU CAGAGAUAAU UAAUGAUU U AAUCUUAACC UAAUUAACUC AUAAUAAAUC CAGCCCUAGA ACAGGGCACA

Macromolecule #13: tRNA-Phe, mitochondrial

• Macromolecule: Name: tRNA-Phe, mitochondrial / type: rna / ID: 13 / Details: CCA end is added post-transcriptionally. / Number of copies: 1
• Source (natural): Organism: pig (pig) / Organ: liver
• Molecular weight: Theoretical: 23.402018 KDa

Sequence

String:

GUUAAUGUAG CUUAAAUUAU CAAAGCAAGG CACUGAAAAU GCCUAGAUGA GCCUCACAGC UCCAUAAACA CCA

Macromolecule #56: 12S ribosomal RNA, mitochondrial

• Macromolecule: Name: 12S ribosomal RNA, mitochondrial / type: rna / ID: 56 / Number of copies: 1
• Source (natural): Organism: Sus scrofa (pig)
• Molecular weight: Theoretical: 308.989062 KDa

Sequence

String:

ACACAGGUUU GGUCCUGGCC UUUCUAUUAA UUCUUAAUAA AAUUACACAU GCAAGUAUCC GCGCCCCGGU GAGAAUGCCC UCCAGAUCU UAAAGAUCAA AAGGAGCAGG UAUCAAGCAC ACCUAUAACG GUAGCUCAUA ACGCCUUGCU CAACCACACC C CCACGGGA AACAGCAGUG AUAAAAAUUA AGCCAUGAAC GAAAGUUUGA CUAAGUUAUA UUAAUUAGAG UUGGUAAAUC UC GUGCCAG CCACCGCGGU CAUACGAUUA ACCCAAAUUA AUAGAUCCAC GGCGUAAAGA GUGUUUAAGA AAAAAAAUCA CAA UAGAGU UAAAUUAUAA CUAAGCUGUA AAAAGCCCUA GUUAAAAUAA AAUAACCCAC GAAAGUGACU CUAAUAAUCC UGAC ACACG AUAGCUAGGA CCCAAACUGG GAUUAGAUAC CCCACUAUGC CUAGCCCUAA ACCCAAAUAG UUACAUAACA AAACU AUUC GCCAGAGUAC UACUCGCAAC UGCCUAAAAC UCAAAGGACU UGGCGGUGCU UCACAUCCAC CUAGAGGAGC CUGUUC UAU AAUCGAUAAA CCCCGAUAGA CCUUACCAAC CCUUGCCAAU UCAGCCUAUA UACCGCCAUC UUCAGCAAAC CCUAAAA AG GAACAAUAGU AAGCACAAUC AUAGCACAUA AAAACGUUAG GUCAAGGUGU AGCUUAUGGG UUGGAAAGAA AUGGGCUA C AUUUUCUACA UAAGAAUAUC CACCACACGA AAGUUUUUAU GAAACUAAAA ACCAAAGGAG GAUUUAGCAG UAAAUCAAG AAUAGAGUGC UUGAUUGAAU AAGGCCAUGA AGCACGCACA CACCGCCCGU CACCCUCCUC AAGCAUGUAG UAAUAAAAAU AACCUAUAU UCAAUUACAC AACCAUGCAA GAAGAGACAA GUCGUAACAA GGUAAGCAUA CUGGAAAGUG UGCUUGGAUU A CC

Macromolecule #72: P-site fMet-tRNAMet, mitochondrial

• Macromolecule: Name: P-site fMet-tRNAMet, mitochondrial / type: rna / ID: 72 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 22.664498 KDa

Sequence

String:

AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A

Macromolecule #73: MT-CO3 mRNA, mitochondrial

• Macromolecule: Name: MT-CO3 mRNA, mitochondrial / type: rna / ID: 73 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 63.946098 KDa

Sequence

String:

AUGACCCACC AAUCACAUGC CUAUCAUAUA GUAAAACCCA GCCCAUGACC CCUAACAGGG GCCCUCUCAG CCCUCCUAAU GACCUCCGG CCUAGCCAUG UGAUUUCACU UCCACUCCAU AACGCUCCUC AUACUAGGCC UACUAACCAA CACACUAACC A UAUACCAA UGAUGGCGCG AUGUAACACG AGAAAGCACA UAC

Macromolecule #90: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 90 / Number of copies: 326 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #91: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 91 / Number of copies: 6 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #92: GUANOSINE-5'-MONOPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-MONOPHOSPHATE / type: ligand / ID: 92 / Number of copies: 2 / Formula: 5GP
• Molecular weight: Theoretical: 363.221 Da

Chemical component information

ChemComp-5GP:
GUANOSINE-5'-MONOPHOSPHATE

Macromolecule #93: SPERMINE

• Macromolecule: Name: SPERMINE / type: ligand / ID: 93 / Number of copies: 3 / Formula: SPM
• Molecular weight: Theoretical: 202.34 Da

Chemical component information

ChemComp-SPM:
SPERMINE

Macromolecule #94: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

• Macromolecule: Name: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / type: ligand / ID: 94 / Number of copies: 1 / Formula: GSP
• Molecular weight: Theoretical: 539.246 Da

Chemical component information

ChemComp-GSP:
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

Macromolecule #95: SODIUM ION

• Macromolecule: Name: SODIUM ION / type: ligand / ID: 95 / Number of copies: 1
• Molecular weight: Theoretical: 22.99 Da

Macromolecule #96: N-FORMYLMETHIONINE

• Macromolecule: Name: N-FORMYLMETHIONINE / type: ligand / ID: 96 / Number of copies: 1 / Formula: FME
• Molecular weight: Theoretical: 177.221 Da

Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE

Macromolecule #97: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 97 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #98: water

• Macromolecule: Name: water / type: ligand / ID: 98 / Number of copies: 5 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.171 mg/mL
• Buffer: pH: 7.6
• Grid: Model: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
• Details: contains 55S mitochondrial ribosome, mitochondrial initiation factor 2, mitochondrial formyl-Met-tRNAMet and MT-CO3 mRNA

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number real images: 13936 / Average exposure time: 1.4 sec. / Average electron dose: 40.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1366787
• CTF correction: Software - Name: RELION (ver. 2.1)

Startup model

Type of model: PDB ENTRY
PDB model - PDB ID:

5aj4

• Final reconstruction: Number classes used: 1 / Applied symmetry - Point group: C₁ (asymmetric) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 139206
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)

Atomic model buiding 1

• Refinement: Space: RECIPROCAL / Protocol: OTHER / Overall B value: 66.8

Output model

PDB-6gaw:
Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the complete 55S ribosome.