National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)
GM130234
United States
Citation
Journal: Nat Struct Mol Biol / Year: 2024 Title: Structure of the γ-tubulin ring complex-capped microtubule. Authors: Amol Aher / Linas Urnavicius / Allen Xue / Kasahun Neselu / Tarun M Kapoor / Abstract: Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules ...Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules assemble with the canonical 13-protofilament architecture, resulting in micrometer-scale α/β-tubulin tracks for intracellular transport that align with, rather than spiral along, the long axis of the filament. We report that the human ~2.3 MDa γ-tubulin ring complex (γ-TuRC), an essential regulator of microtubule formation that contains 14 γ-tubulins, selectively nucleates 13-protofilament microtubules. Cryogenic electron microscopy reconstructions of γ-TuRC-capped microtubule minus ends reveal the extensive intra-domain and inter-domain motions of γ-TuRC subunits that accommodate luminal bridge components and establish lateral and longitudinal interactions between γ-tubulins and α-tubulins. Our structures suggest that γ-TuRC, an inefficient nucleation template owing to its splayed conformation, can transform into a compacted cap at the microtubule minus end and set the lattice architecture of cellular microtubules.
Entire : Gamma tubulin ring complex bound to microtubule minus end
Entire
Name: Gamma tubulin ring complex bound to microtubule minus end
Components
Complex: Gamma tubulin ring complex bound to microtubule minus end
Protein or peptide: Tubulin alpha-1B chain
Protein or peptide: Tubulin beta-3 chain
Protein or peptide: TUBGCP6 protein
Protein or peptide: Gamma-tubulin complex component 3
Protein or peptide: Mitotic-spindle organizing protein 1
Protein or peptide: Actin, cytoplasmic 1
Protein or peptide: Isoform 3 of Gamma-tubulin complex component 2
Protein or peptide: Isoform 2 of Gamma-tubulin complex component 4
Protein or peptide: Gamma-tubulin complex component 5
Protein or peptide: Tubulin gamma-1 chain
+
Supramolecule #1: Gamma tubulin ring complex bound to microtubule minus end
Supramolecule
Name: Gamma tubulin ring complex bound to microtubule minus end type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)
Organism: Homo sapiens (human)
+
Macromolecule #1: Tubulin alpha-1B chain
Macromolecule
Name: Tubulin alpha-1B chain / type: protein_or_peptide / ID: 1 / Number of copies: 13 / Enantiomer: LEVO EC number: Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement
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