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データを開く
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基本情報
| 登録情報 | ![]() | |||||||||
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| タイトル | Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site | |||||||||
マップデータ | Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site | |||||||||
試料 |
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キーワード | Transcription / translation / coupling / NusG / NusA / ops / TTC | |||||||||
| 機能・相同性 | 機能・相同性情報transcription elongation-coupled chromatin remodeling / stringent response / RNA polymerase complex / negative regulation of cytoplasmic translational initiation / submerged biofilm formation / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type RNA polymerase core enzyme binding / cytosolic DNA-directed RNA polymerase complex ...transcription elongation-coupled chromatin remodeling / stringent response / RNA polymerase complex / negative regulation of cytoplasmic translational initiation / submerged biofilm formation / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type RNA polymerase core enzyme binding / cytosolic DNA-directed RNA polymerase complex / misfolded RNA binding / Group I intron splicing / transcription elongation factor complex / RNA folding / bacterial-type flagellum-dependent cell motility / nitrate assimilation / translational termination / positive regulation of ribosome biogenesis / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / regulation of DNA-templated transcription elongation / cytosolic ribosome assembly / ribosome assembly / assembly of large subunit precursor of preribosome / cell motility / transcription antitermination / DNA-directed RNA polymerase complex / regulation of cell growth / DNA-templated transcription initiation / translational initiation / DNA-templated transcription termination / maintenance of translational fidelity / mRNA 5'-UTR binding / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / large ribosomal subunit / response to heat / transferase activity / ribosomal small subunit assembly / ribosome binding / ribosomal small subunit biogenesis / protein-containing complex assembly / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / intracellular iron ion homeostasis / cytoplasmic translation / tRNA binding / protein dimerization activity / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / DNA-binding transcription factor activity / ribonucleoprotein complex / response to antibiotic / nucleotide binding / mRNA binding / magnesium ion binding / DNA-templated transcription / DNA binding / RNA binding / zinc ion binding / membrane / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
| 生物種 | ![]() | |||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | |||||||||
データ登録者 | Molodtsov V / Wang C / Ebright RH | |||||||||
| 資金援助 | 米国, 1件
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引用 | ジャーナル: Proc Natl Acad Sci U S A / 年: 2026タイトル: Structural basis of long-range transcription-translation coupling. 著者: Chengyuan Wang / Vadim Molodtsov / Shashank Shandilya / Linlin You / Jing Zhang / Konstantin Kuznedelov / Bryce E Nickels / Jason T Kaelber / Gregor Blaha / Richard H Ebright / ![]() 要旨: Structures recently have been reported of molecular assemblies that mediate transcription-translation coupling in . In these molecular assemblies, termed "coupled transcription-translation complexes" ...Structures recently have been reported of molecular assemblies that mediate transcription-translation coupling in . In these molecular assemblies, termed "coupled transcription-translation complexes" or "TTC-B," RNA polymerase (RNAP) directly interacts with the ribosome, the transcription elongation factor NusG or its paralog RfaH forms a bridge between RNAP and ribosome, and the transcription elongation factor NusA optionally forms a second bridge between RNAP and ribosome. Here, we report structures of coupled transcription-translation complexes having mRNA spacers between RNAP and ribosome longer than the maximum-length mRNA spacer compatible with formation of TTC-B. The results define a class of coupled transcription-translation complex, termed "TTC-LC," where "LC" denotes "long-range coupling." TTC-LC differs from TTC-B by a ~60° rotation and ~70 Å translation of RNAP relative to ribosome, resulting in loss of direct interactions between RNAP and ribosome and creation of a ~70 Å gap between RNAP and ribosome. TTC-LC accommodates long mRNA spacers by looping out mRNA from the gap between RNAP and ribosome. We present evidence that TTC-LC is a functional intermediate in assembling and disassembling TTC-B, mediating pre-TTC-B transcription-translation coupling before a ribosome catches up to RNAP, and mediating post-TTC-B transcription-translation coupling after a ribosome stops moving and RNAP continues moving. We show that TTC-B, but not TTC-LC, is severely defective in RNA-hairpin-dependent transcription termination, and that both TTC-B and TTC-LC are severely defective in Rho-dependent transcription termination. | |||||||||
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
| マップデータ | emd_43390.map.gz | 408.4 MB | EMDBマップデータ形式 | |
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| ヘッダ (付随情報) | emd-43390-v30.xml emd-43390.xml | 99.7 KB 99.7 KB | 表示 表示 | EMDBヘッダ |
| 画像 | emd_43390.png | 78.7 KB | ||
| Filedesc metadata | emd-43390.cif.gz | 20.7 KB | ||
| その他 | emd_43390_half_map_1.map.gz emd_43390_half_map_2.map.gz | 412.2 MB 412.2 MB | ||
| アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-43390 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-43390 | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 8vorMC ![]() 8vkvC ![]() 8vl1C ![]() 8vooC ![]() 8vopC ![]() 8voqC ![]() 8vosC ![]() 9w0nC ![]() 9y79C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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| 「今月の分子」の関連する項目 |
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マップ
| ファイル | ダウンロード / ファイル: emd_43390.map.gz / 形式: CCP4 / 大きさ: 512 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| 注釈 | Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 51 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site | ||||||||||||||||||||||||||||||||||||
| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 1.04 Å | ||||||||||||||||||||||||||||||||||||
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| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
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-添付データ
-ハーフマップ: unmasked half map 1
| ファイル | emd_43390_half_map_1.map | ||||||||||||
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| 注釈 | unmasked half map 1 | ||||||||||||
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| 密度ヒストグラム |
-ハーフマップ: unmasked half map 2
| ファイル | emd_43390_half_map_2.map | ||||||||||||
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| 注釈 | unmasked half map 2 | ||||||||||||
| 投影像・断面図 |
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| 密度ヒストグラム |
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試料の構成要素
+全体 : Escherichia coli transcription-translation loosely coupled comple...
+超分子 #1: Escherichia coli transcription-translation loosely coupled comple...
+分子 #1: Ribosomal protein L21
+分子 #2: 50S ribosomal protein L22
+分子 #3: 50S ribosomal protein L23
+分子 #4: 50S ribosomal protein L24
+分子 #5: 50S ribosomal protein L25
+分子 #9: 50S ribosomal protein L10
+分子 #11: DNA-directed RNA polymerase subunit beta
+分子 #12: Transcription termination/antitermination protein NusG
+分子 #13: DNA-directed RNA polymerase subunit alpha
+分子 #14: DNA-directed RNA polymerase subunit beta'
+分子 #15: DNA-directed RNA polymerase subunit omega
+分子 #16: Transcription termination/antitermination protein NusA
+分子 #17: 30S ribosomal protein S18
+分子 #19: 30S ribosomal protein S20
+分子 #20: 30S ribosomal protein S21
+分子 #21: 30S ribosomal protein S2
+分子 #22: 30S ribosomal protein S1
+分子 #23: 30S ribosomal protein S3
+分子 #24: 30S ribosomal protein S4
+分子 #25: 30S ribosomal protein S5
+分子 #26: 30S ribosomal protein S6
+分子 #27: 30S ribosomal protein S7
+分子 #28: 30S ribosomal protein S8
+分子 #29: 30S ribosomal protein S9
+分子 #30: 30S ribosomal protein S10
+分子 #31: 30S ribosomal protein S11
+分子 #32: 30S ribosomal protein S12
+分子 #33: 30S ribosomal protein S14
+分子 #34: Small ribosomal subunit protein uS15
+分子 #35: 30S ribosomal protein S16
+分子 #36: 30S ribosomal protein S17
+分子 #37: 30S ribosomal protein S19
+分子 #38: 30S ribosomal protein S13
+分子 #39: 50S ribosomal protein L11
+分子 #40: 50S ribosomal protein L7/L12
+分子 #42: 50S ribosomal protein L27
+分子 #43: 50S ribosomal protein L28
+分子 #45: 50S ribosomal protein L29
+分子 #46: 50S ribosomal protein L30
+分子 #47: 50S ribosomal protein L31
+分子 #48: 50S ribosomal protein L2
+分子 #49: 50S ribosomal protein L32
+分子 #50: 50S ribosomal protein L3
+分子 #51: 50S ribosomal protein L33
+分子 #52: 50S ribosomal protein L4
+分子 #53: 50S ribosomal protein L34
+分子 #54: 50S ribosomal protein L5
+分子 #55: 50S ribosomal protein L35
+分子 #56: 50S ribosomal protein L6
+分子 #57: 50S ribosomal protein L36
+分子 #58: 50S ribosomal protein L9
+分子 #59: 50S ribosomal protein L13
+分子 #60: 50S ribosomal protein L14
+分子 #61: 50S ribosomal protein L15
+分子 #62: 50S ribosomal protein L16
+分子 #63: 50S ribosomal protein L17
+分子 #64: 50S ribosomal protein L18
+分子 #65: 50S ribosomal protein L19
+分子 #66: 50S ribosomal protein L20
+分子 #6: NT DNA
+分子 #7: T DNA
+分子 #8: mRNA with 51 nt long spacer
+分子 #10: E-site and P-site tRNA (fMet)
+分子 #18: 16S rRNA
+分子 #41: 23S rRNA
+分子 #44: 5S rRNA
+分子 #67: MAGNESIUM ION
+分子 #68: ZINC ION
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
| 試料の集合状態 | particle |
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試料調製
| 緩衝液 | pH: 7.5 |
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| 凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
| 顕微鏡 | FEI TALOS ARCTICA |
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| 撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 50.0 e/Å2 |
| 電子線 | 加速電圧: 200 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.25 µm |
| 実験機器 | ![]() モデル: Talos Arctica / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
データ登録者
米国, 1件
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解析
FIELD EMISSION GUN
