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- PDB-8vos: Escherichia coli transcription-translation loosely coupled comple... -

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Basic information

Entry
Database: PDB / ID: 8vos
TitleEscherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site
Components
  • (30S ribosomal protein ...) x 3
  • (50S ribosomal protein ...) x 17
  • (DNA-directed RNA polymerase subunit ...) x 4
  • (Large ribosomal subunit protein ...) x 14
  • (Small ribosomal subunit protein ...) x 18
  • (Transcription termination/antitermination protein ...) x 2
  • 16S rRNA
  • 23S rRNA
  • 5S rRNA
  • E-site and P-site tRNA (fMet)
  • NT DNA
  • Ribosomal protein L21
  • T DNA
  • mRNA with 60 nt long spacer
KeywordsTRANSCRIPTION / translation / coupling / NusG / NusA / ops / TTC
Function / homology
Function and homology information


DNA-templated transcription elongation / stringent response / RNA polymerase complex / negative regulation of cytoplasmic translational initiation / submerged biofilm formation / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type RNA polymerase core enzyme binding / cytosolic DNA-directed RNA polymerase complex ...DNA-templated transcription elongation / stringent response / RNA polymerase complex / negative regulation of cytoplasmic translational initiation / submerged biofilm formation / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type RNA polymerase core enzyme binding / cytosolic DNA-directed RNA polymerase complex / misfolded RNA binding / Group I intron splicing / RNA folding / bacterial-type flagellum-dependent cell motility / nitrate assimilation / translational termination / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / positive regulation of RNA splicing / regulation of DNA-templated transcription elongation / transcription elongation factor complex / cytosolic ribosome assembly / ribosome assembly / assembly of large subunit precursor of preribosome / DNA-directed RNA polymerase complex / cell motility / transcription antitermination / DNA-templated transcription initiation / translational initiation / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / large ribosomal subunit / transferase activity / ribosomal small subunit assembly / response to heat / ribosome binding / ribosomal small subunit biogenesis / protein-containing complex assembly / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / intracellular iron ion homeostasis / cytoplasmic translation / tRNA binding / protein dimerization activity / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / DNA-binding transcription factor activity / ribonucleoprotein complex / response to antibiotic / negative regulation of DNA-templated transcription / nucleotide binding / mRNA binding / magnesium ion binding / DNA-templated transcription / DNA binding / RNA binding / zinc ion binding / membrane / metal ion binding / cytosol / cytoplasm
Similarity search - Function
: / : / NusA-like second KH domain / Transcription termination factor NusA, C-terminal duplication / Transcription termination factor NusA / Transcription factor NusA, N-terminal / KH domain, NusA-like / NusA, N-terminal domain superfamily / NusA N-terminal domain / NusA-like first KH domain ...: / : / NusA-like second KH domain / Transcription termination factor NusA, C-terminal duplication / Transcription termination factor NusA / Transcription factor NusA, N-terminal / KH domain, NusA-like / NusA, N-terminal domain superfamily / NusA N-terminal domain / NusA-like first KH domain / Transcription termination/antitermination protein NusA, bacterial / Ribosomal protein S1 / Ribosomal protein S1-like / : / Transcription antitermination protein, NusG / Transcription antitermination protein, NusG, bacteria, conserved site / Transcription termination factor nusG signature. / Ribosomal protein L7/L12, oligomerisation / Ribosomal protein L7/L12, oligomerisation domain superfamily / Ribosomal protein L7/L12 dimerisation domain / Ribosomal protein L7/L12 / Ribosomal protein L7/L12, C-terminal / Ribosomal protein L7/L12 C-terminal domain / NusG-like / Transcription termination factor nusG / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / Type-1 KH domain profile. / NusG, N-terminal domain superfamily / DNA repair Rad51/transcription factor NusA, alpha-helical / Ribosomal protein L10, eubacterial, conserved site / Ribosomal protein L10 signature. / SAM-like Helix-hairpin-helix tandem / Ribosomal protein L10 / : / S1 domain profile. / Ribosomal protein L11, bacterial-type / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase beta subunit, bacterial-type / DNA-directed RNA polymerase, alpha subunit / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 superfamily / S1 domain / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L10-like domain superfamily / Ribosomal protein L10P / Ribosomal protein L10 / Ribosomal protein S21 / Ribosomal protein L9 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L9, C-terminal domain superfamily / : / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L28/L24 superfamily
Similarity search - Domain/homology
: / : / : / : / DNA / DNA (> 10) / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) ...: / : / : / : / DNA / DNA (> 10) / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL28 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein bL20 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL12 / Large ribosomal subunit protein bL33 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit omega / DNA-directed RNA polymerase subunit beta' / DNA-directed RNA polymerase subunit beta / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Transcription termination/antitermination protein NusA / Transcription termination/antitermination protein NusG / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein bS1 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein bL25
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
synthetic construct (others)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.6 Å
AuthorsMolodtsov, V. / Wang, C. / Ebright, R.H.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2026
Title: Structural basis of long-range transcription-translation coupling.
Authors: Chengyuan Wang / Vadim Molodtsov / Shashank Shandilya / Linlin You / Jing Zhang / Konstantin Kuznedelov / Bryce E Nickels / Jason T Kaelber / Gregor Blaha / Richard H Ebright /
Abstract: Structures recently have been reported of molecular assemblies that mediate transcription-translation coupling in . In these molecular assemblies, termed "coupled transcription-translation complexes" ...Structures recently have been reported of molecular assemblies that mediate transcription-translation coupling in . In these molecular assemblies, termed "coupled transcription-translation complexes" or "TTC-B," RNA polymerase (RNAP) directly interacts with the ribosome, the transcription elongation factor NusG or its paralog RfaH forms a bridge between RNAP and ribosome, and the transcription elongation factor NusA optionally forms a second bridge between RNAP and ribosome. Here, we report structures of coupled transcription-translation complexes having mRNA spacers between RNAP and ribosome longer than the maximum-length mRNA spacer compatible with formation of TTC-B. The results define a class of coupled transcription-translation complex, termed "TTC-LC," where "LC" denotes "long-range coupling." TTC-LC differs from TTC-B by a ~60° rotation and ~70 Å translation of RNAP relative to ribosome, resulting in loss of direct interactions between RNAP and ribosome and creation of a ~70 Å gap between RNAP and ribosome. TTC-LC accommodates long mRNA spacers by looping out mRNA from the gap between RNAP and ribosome. We present evidence that TTC-LC is a functional intermediate in assembling and disassembling TTC-B, mediating pre-TTC-B transcription-translation coupling before a ribosome catches up to RNAP, and mediating post-TTC-B transcription-translation coupling after a ribosome stops moving and RNAP continues moving. We show that TTC-B, but not TTC-LC, is severely defective in RNA-hairpin-dependent transcription termination, and that both TTC-B and TTC-LC are severely defective in Rho-dependent transcription termination.
History
DepositionJan 15, 2024Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 23, 2025Provider: repository / Type: Initial release
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
0: Ribosomal protein L21
1: 50S ribosomal protein L22
2: 50S ribosomal protein L23
3: 50S ribosomal protein L24
4: Large ribosomal subunit protein bL25
5: NT DNA
6: T DNA
7: mRNA with 60 nt long spacer
9: 50S ribosomal protein L10
A: E-site and P-site tRNA (fMet)
AA: DNA-directed RNA polymerase subunit beta
AB: Transcription termination/antitermination protein NusG
AC: DNA-directed RNA polymerase subunit alpha
AD: DNA-directed RNA polymerase subunit alpha
AE: DNA-directed RNA polymerase subunit beta'
AF: DNA-directed RNA polymerase subunit omega
AG: Transcription termination/antitermination protein NusA
B: E-site and P-site tRNA (fMet)
C: Small ribosomal subunit protein bS18
D: 16S rRNA
E: Small ribosomal subunit protein bS20
F: Small ribosomal subunit protein bS21
G: 30S ribosomal protein S2
H: Small ribosomal subunit protein bS1
I: Small ribosomal subunit protein uS3
J: Small ribosomal subunit protein uS4
K: Small ribosomal subunit protein uS5
L: Small ribosomal subunit protein bS6
M: Small ribosomal subunit protein uS7
N: Small ribosomal subunit protein uS8
O: Small ribosomal subunit protein uS9
P: Small ribosomal subunit protein uS10
Q: 30S ribosomal protein S11
R: 30S ribosomal protein S12
S: Small ribosomal subunit protein uS14
T: Small ribosomal subunit protein uS15
U: Small ribosomal subunit protein bS16
V: Small ribosomal subunit protein uS17
W: Small ribosomal subunit protein uS19
X: Small ribosomal subunit protein uS13
Y: 50S ribosomal protein L11
Z: Large ribosomal subunit protein bL12
a: 23S rRNA
b: Large ribosomal subunit protein bL27
c: 50S ribosomal protein L28
d: 5S rRNA
e: Large ribosomal subunit protein uL29
f: 50S ribosomal protein L30
g: 50S ribosomal protein L31
h: 50S ribosomal protein L2
i: 50S ribosomal protein L32
j: 50S ribosomal protein L3
k: 50S ribosomal protein L33
l: Large ribosomal subunit protein uL4
m: 50S ribosomal protein L34
n: Large ribosomal subunit protein uL5
o: 50S ribosomal protein L35
p: Large ribosomal subunit protein uL6
q: 50S ribosomal protein L36
r: Large ribosomal subunit protein bL9
s: Large ribosomal subunit protein uL13
t: Large ribosomal subunit protein uL14
u: Large ribosomal subunit protein uL15
v: 50S ribosomal protein L16
w: Large ribosomal subunit protein bL17
x: Large ribosomal subunit protein uL18
y: Large ribosomal subunit protein bL19
z: 50S ribosomal protein L20
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,806,38871
Polymers2,806,23368
Non-polymers1553
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 1 types, 1 molecules 0

#1: Protein Ribosomal protein L21


Mass: 11586.374 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: UniProt: C3SSG2

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50S ribosomal protein ... , 17 types, 17 molecules 1239Ycfghijkmoqvz

#2: Protein 50S ribosomal protein L22 / Large ribosomal subunit protein uL22


Mass: 12253.359 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplV, eryB, b3315, JW3277 / Production host: Escherichia coli (E. coli) / References: UniProt: P61175
#3: Protein 50S ribosomal protein L23


Mass: 11222.160 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, BF481_ ...Gene: rplW, rplW_1, A5U30_004552, A8502_004023, A9X72_02185, ACN81_25520, ACU57_20960, AM464_09220, AW118_24900, AWP47_12645, B6R15_004069, B6R31_004968, BANRA_04328, BCB93_004293, BER14_25190, BF481_004930, BG944_001133, BGM66_003593, BJI68_08675, BJJ90_02125, BK292_20505, BK383_24645, BKL28_004543, BTB68_004356, BTQ06_10690, BvCmsKKP061_03030, BvCmsSIP010_02685, BXT93_05475, C0P57_002668, C1Q91_004904, C2121_004162, C2M16_25405, C2R31_004633, C3F40_19720, C9E67_02725, C9Z68_22635, CA593_09650, CF22_004849, CG692_20185, CG704_20870, CG831_003649, CIG67_10675, CQ986_004881, CR538_02420, CR539_21900, CR628_001366, CTR35_003630, CV83915_02780, CY655_21320, D1912_09370, D3C88_31150, D3G36_23050, D4M65_20655, D4N09_20255, D9D43_22550, D9E49_05240, D9J61_16735, DD762_23630, DIV22_15175, DNQ45_03840, DNX30_25395, DQ87_004776, DS732_24345, DTL43_20915, DU321_12085, E4K51_21575, E5H86_24440, E6D34_21675, EAI46_07560, EC95NR1_02709, ECs4183, EEP55_004492, EIA08_24040, EIZ93_12385, EN85_004349, EPS76_07130, EPS97_20010, EWK56_25080, ExPECSC038_03640, F7F11_22640, F7N46_23925, F9407_19210, F9413_21150, F9461_25570, F9B07_24600, FEJ01_22430, FIJ20_21110, FJQ40_18390, FKO60_25720, FOI11_019175, FOI11_04005, FPI65_20390, FPS11_25345, FTV92_20630, FV293_22380, FVB16_04955, FWK02_12650, FZN31_02550, FZU14_21905, G3V95_19930, G3W53_21040, G4A38_21595, G4A47_20865, G5603_24670, G8C01_22470, G9448_16990, GAI89_24745, GAJ12_24635, GGB84_004451, GJ11_21470, GKF66_21630, GNW61_16715, GOP25_22790, GP711_23390, GP954_00860, GP965_04380, GP975_01270, GP979_01920, GQA06_03865, GQM04_10650, GQM13_25285, GQM21_11315, GQN34_23350, GQW07_21465, GRO95_20730, GRW05_09145, GRW24_04670, GRW56_00380, GRW57_03455, GTP92_23110, GUC01_21265, H0O72_19500, HCQ42_003932, HEP30_018720, HEP34_004754, HHH44_004519, HI055_004156, HIE29_005203, HJQ60_004995, HLX92_09990, HLZ50_22095, HMV95_19690, HV109_02105, HV146_21755, HV209_14630, HVW04_17450, HVW43_18585, HVY77_02090, HVZ29_19180, HX086_19490, HX136_02110, I0E88_004501, I6H00_20840, I6H02_12075, IFB95_004822, IUC12_004844, J0541_004413, J5U05_004071, JNP96_25390, KV259_004342, KV317_004314, KV371_004328, KV406_004321, KV449_004356, KV455_004319, KV463_004341, KV469_004334, KV499_004405, KV500_004292, NCTC10082_02823, NCTC10089_00486, NCTC10090_03511, NCTC10418_00674, NCTC10429_01077, NCTC10764_03847, NCTC10767_01500, NCTC10865_00642, NCTC10974_00533, NCTC11112_01052, NCTC11124_04730, NCTC11126_02754, NCTC11181_02722, NCTC11341_01905, NCTC12950_00463, NCTC13148_03456, NCTC4450_02459, NCTC7927_00535, NCTC7928_02533, NCTC8009_01493, NCTC8179_05925, NCTC8333_00473, NCTC8500_00318, NCTC8621_00492, NCTC8622_00522, NCTC8959_03277, NCTC8960_03054, NCTC8985_04965, NCTC9001_04236, NCTC9044_01376, NCTC9045_00549, NCTC9073_00398, NCTC9075_00708, NCTC9081_00901, NCTC9117_00740, NCTC9702_00521, NCTC9706_02721, NCTC9962_06781, NEP60_26375, O5851_12245, RG28_21695, SAMEA3752557_01968, TUM18780_04200
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQV2
#4: Protein 50S ribosomal protein L24


Mass: 11339.250 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_001124, ...Gene: rplX, A8502_004032, A9X72_02230, ACN81_25475, ACU57_21005, AM464_09175, AW118_24855, AWP47_12690, B6R15_004078, B6R31_004977, BANRA_04319, BCB93_004284, BER14_25145, BF481_004939, BG944_001124, BGM66_003602, BJI68_08720, BJJ90_02170, BK292_20460, BK383_24690, BKL28_004552, BTB68_004365, BTQ06_10645, BvCmsKKP061_03021, BvCmsSIP010_02676, BXT93_05520, C0P57_002659, C1Q91_004913, C2121_004171, C2M16_25450, C2R31_004624, C3F40_19765, C9E67_02770, C9Z68_22590, CA593_09695, CF22_004858, CG704_20915, CG831_003640, CIG67_10720, CQ986_004890, CR538_02465, CR539_21855, CR628_001357, CTR35_003621, CV83915_02789, CY655_21275, D4M65_20610, D9D43_22595, DD762_23585, DIV22_15220, DNQ45_03795, DNX30_25440, DQ87_004785, DS732_24300, DTL43_20960, DU321_12040, E4K51_21530, E5H86_24485, E6D34_21720, EAI46_07515, EC95NR1_02700, ECs4174, EEP55_004501, EIA08_23995, EIZ93_12430, EN85_004358, EPS97_20055, ExPECSC038_03649, F7F11_22685, F7N46_23970, F9407_19255, F9413_21195, F9461_25615, F9B07_24645, FEJ01_22475, FIJ20_21155, FJQ40_18345, FKO60_25675, FOI11_019220, FOI11_03960, FPS11_25390, FTV92_20675, FTV93_20860, FV293_22425, FVB16_04910, FWK02_06310, FZN31_02595, FZU14_21860, G3V95_19885, G4A38_21550, G4A47_20820, G5603_24625, G8C01_22515, G9448_17035, GAI89_24790, GAJ12_24680, GJ11_21425, GKF66_21585, GNW61_16670, GOP25_22745, GP954_00905, GP965_04335, GP975_01225, GP979_01965, GQA06_03910, GQM04_10605, GQM13_25240, GQM21_11270, GQW07_21510, GRO95_20775, GRW05_09100, GRW24_04715, GRW56_00335, GRW57_03500, GTP92_23155, GUC01_21220, H0O72_19455, HCQ42_003941, HEP30_018675, HEP34_004763, HHH44_004528, HI055_004147, HJQ60_005004, HLX92_10035, HLZ50_22140, HMV95_19645, HV109_02150, HV146_21710, HV209_14675, HVW04_17495, HVW43_18630, HVY77_02135, HVZ29_19135, HX086_19445, HX136_02155, I0E88_004510, I6H00_20885, I6H02_12030, IUC12_004853, J0541_004404, J5U05_004080, JNP96_25345, KV259_004351, KV317_004323, KV371_004337, KV406_004330, KV449_004365, KV455_004328, KV463_004350, KV469_004343, KV499_004414, KV500_004301, NCTC10082_02832, NCTC10418_00684, NCTC10764_03856, NCTC10865_00652, NCTC10974_00542, NCTC11124_04721, NCTC11181_02731, NCTC11341_01896, NCTC12950_00472, NCTC7922_04049, NCTC7927_00544, NCTC8009_01504, NCTC8179_05935, NCTC8333_00482, NCTC8500_00327, NCTC8621_00501, NCTC8622_00513, NCTC8960_03063, NCTC8985_04975, NCTC9044_01365, NCTC9045_00558, NCTC9077_00597, NCTC9117_00749, NCTC9702_00530, NCTC9706_02730, NEP60_26330, O5851_12290, RG28_21650, SAMEA3752557_01959, TUM18780_04290, WR15_19645
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQZ7
#9: Protein 50S ribosomal protein L10 / 50S ribosomal protein L8 / Large ribosomal subunit protein uL10


Mass: 17736.596 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplJ, b3985, JW3948 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7J3
#39: Protein 50S ribosomal protein L11 / Large ribosomal subunit protein uL11


Mass: 14894.362 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplK, relC, b3983, JW3946 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7J7
#43: Protein 50S ribosomal protein L28


Mass: 9027.551 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmB, ECVG_03476 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A1X3JA73
#46: Protein 50S ribosomal protein L30


Mass: 6554.820 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_004085, ...Gene: rpmD, A5U30_004536, A6592_25715, A8499_003316, A8502_004039, A9X72_02265, AC065_15925, ACN81_25440, ACU57_21040, AM464_09140, APX88_16155, AW118_24820, AWP47_12725, B6R12_002823, B6R15_004085, B6R17_004958, B6R31_004984, B6R48_005051, B6R87_004961, BANRA_04312, BB540_01660, BER14_25110, BF481_004946, BG944_001117, BGM66_003609, BHS81_19755, BJI68_08755, BJJ90_02205, BK292_20425, BK383_24725, BKL28_004559, BMT50_12295, BMT91_19990, BR158_003774, BTB68_004372, BTQ06_10610, BUO55_004713, BvCmsKKP061_03014, BvCmsSIP010_02669, BXT93_05555, BZL69_25950, C0P57_002652, C1Q91_004920, C2121_004178, C2M16_25485, C2R31_004617, C3F40_19800, C5N07_23570, C9E67_02805, C9Z68_22555, CA593_09730, CCS08_00135, CCU84_002918, CDL36_25590, CF22_004865, CG692_20265, CG704_20950, CG831_003633, CIG67_10755, CQ986_004897, CQB02_00630, CR538_02500, CR539_21820, CR628_001350, CRM83_22925, CTR35_003614, CV83915_02796, CWS33_24860, CX938_003815, CY655_21240, D1912_11425, D3G36_23130, D4M65_20575, D4N09_20175, D6T60_05695, D9D43_22630, D9E34_23605, D9E49_05160, D9H94_20830, D9J03_20055, D9J61_16655, DD762_23550, DIV22_15255, DNQ45_03760, DNX30_25475, DS732_24265, DTL43_20995, DTL90_24695, DTM45_24550, DU321_12005, E0I52_20145, E4K51_21495, E5H86_24520, E6D34_21755, EA239_22795, EAI46_07480, EB534_RS01705, EC95NR1_02693, ECs4167, EIA08_23960, EIZ93_12465, EN85_004365, EPS76_07210, EPS97_20090, ERS085406_04406, ERS139208_04354, EWK56_25000, ExPECSC038_03656, F3P10_05565, F7F11_22720, F7N46_24005, F9413_21230, F9461_25650, F9B07_24680, FA868_22750, FEJ01_22510, FFF58_25450, FGG80_25130, FHD44_21270, FHQ97_20115, FIJ20_21190, FJQ40_18310, FKO60_25640, FN993_004711, FOI11_019255, FOI11_03925, FPI65_20310, FPS11_25425, FTV92_20710, FTV93_20895, FV293_22460, FVB16_04875, FWK02_06275, FZN31_02630, FZU14_21825, G3V95_19850, G3W53_20960, G4A38_21515, G4A47_20785, G5603_24590, G9448_17070, GAI89_24825, GAJ12_24715, GAJ26_22015, GGB84_004467, GIB53_19395, GJ11_21390, GKF52_21245, GKF66_21550, GKF89_18475, GNW61_16635, GOP14_004273, GOP25_22710, GP711_23310, GP954_00940, GP965_04300, GP975_01190, GP979_02000, GQA06_03945, GQM04_10570, GQM13_25205, GQM17_19920, GQM21_11235, GQN34_23270, GQW07_21545, GRO95_20810, GRW05_09065, GRW24_04750, GRW56_00300, GRW57_03535, GSM54_23325, GSY44_20005, GUC01_21185, GUI33_20050, H0O53_20825, H0O72_19420, H0P11_20920, H5Z35_004789, H6Y26_003164, HCQ42_003948, HEP30_018640, HEP34_004770, HHH44_004535, HI055_004140, HIE29_005187, HJY54_004753, HKA49_005035, HLQ92_22595, HLV18_23335, HLX92_10070, HLZ50_22175, HMV95_19610, HMW38_23110, HV109_02185, HV146_21675, HV209_14710, HVV39_09270, HVW04_17530, HVW43_18665, HVY77_02170, HVZ29_19100, HVZ30_02050, HVZ71_02355, HX136_02190, I6H00_20920, I6H02_11995, IFB95_004806, IUD45_003540, J0541_004397, J4S20_004802, J5U05_004087, J7T50_001453, JFD_01884, JFD_03713, JNP96_25310, KQ660_11775, NCTC10082_02839, NCTC10089_00502, NCTC10090_03527, NCTC10418_00690, NCTC10429_01095, NCTC10487_00445, NCTC10764_03863, NCTC10767_01516, NCTC10865_00661, NCTC10974_00551, NCTC11126_02771, NCTC11127_03223, NCTC11181_02738, NCTC11341_01889, NCTC12950_00479, NCTC13127_00786, NCTC4450_02475, NCTC7922_04042, NCTC7927_00551, NCTC7928_02516, NCTC8009_01511, NCTC8179_05942, NCTC8333_00489, NCTC8450_04937, NCTC8500_00335, NCTC8621_00508, NCTC8622_00507, NCTC8959_03261, NCTC8960_03070, NCTC8985_04982, NCTC9001_04254, NCTC9044_01356, NCTC9045_00566, NCTC9050_03611, NCTC9071_00483, NCTC9073_00380, NCTC9075_00724, NCTC9077_00604, NCTC9081_00918, NCTC9111_00867, NCTC9117_00756, NCTC9702_00537, NCTC9706_02737, NCTC9775_04003, NCTC9777_01891, NEP60_26295, OS110_25120, RG28_21615, SAMEA3472044_01162, SAMEA3472056_05411, SAMEA3752557_01952, SAMEA3753106_04634, TUM18780_04360, WR15_19610
Production host: Escherichia coli (E. coli) / References: UniProt: C3SR32
#47: Protein 50S ribosomal protein L31 / Large ribosomal subunit protein bL31-A


Mass: 7887.117 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmE, b3936, JW3907 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7M9
#48: Protein 50S ribosomal protein L2 / Large ribosomal subunit protein uL2


Mass: 29923.619 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplB, b3317, JW3279 / Production host: Escherichia coli (E. coli) / References: UniProt: P60422
#49: Protein 50S ribosomal protein L32


Mass: 6463.445 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, BF481_ ...Gene: rpmF, rpmF_1, A5U30_002830, A8502_000661, A9X72_15435, ACN81_09130, ACU57_04225, AM464_23480, AW118_12110, AWP47_13180, B6R15_001792, B6R31_003138, BANRA_01624, BCB93_002043, BER14_10210, BF481_002131, BG944_003607, BGM66_002729, BJI68_20640, BJJ90_15780, BK292_02505, BK383_03640, BKL28_002219, BTB68_003153, BTQ06_08080, BvCmsKKP061_02173, BvCmsSIP010_04163, BXT93_21825, C0P57_001747, C1Q91_003167, C2121_002257, C2M16_23600, C2R31_002348, C3F40_06530, C9E67_18185, C9Z68_07340, CA593_23040, CF22_003088, CG692_05970, CG704_04105, CG831_001542, CIG67_21935, CQ986_001084, CR538_15635, CR539_10135, CR628_002785, CTR35_002374, CV83915_00494, CY655_06595, D1912_08350, D3C88_19355, D3G36_11860, D4M65_04795, D4N09_11015, D9D43_11825, D9E49_23135, D9J61_04035, DD762_03625, DIV22_29035, DNQ45_10015, DNX30_12140, DQ87_002042, DS732_10505, DTL43_16270, DU321_22840, E4K51_18340, E5H86_12535, E6D34_10935, EAI46_03975, EC95NR1_05485, ECs1467, EEP55_000822, EIA08_02925, EIZ93_13150, EN85_002840, EPS76_00770, EPS97_06370, EWK56_20060, ExPECSC038_03490, F7F11_10320, F7N46_02450, F9413_18150, F9461_13585, F9B07_16900, FEJ01_12565, FIJ20_07300, FJQ40_00940, FKO60_17370, FOI11_007500, FOI11_14470, FPI65_06540, FPS11_20235, FTV92_08325, FTV93_08485, FV293_19565, FVB16_04695, FWK02_15570, FZN31_14620, FZU14_20010, G3V95_08050, G3W53_15755, G4A38_16050, G4A47_15410, G5603_00600, G8C01_10030, G9448_05130, GAI89_13280, GAJ12_13930, GGB84_000483, GJ11_06555, GKF66_05180, GNW61_10640, GOP25_17405, GP711_16475, GP954_12815, GP965_04045, GP975_03585, GP979_13150, GQA06_08430, GQM04_02110, GQM13_14340, GQM21_06935, GQN34_10245, GQW07_08880, GRO95_10420, GRW05_02410, GRW24_27585, GRW56_06550, GRW57_07595, GTP92_19120, GUC01_01680, H0O72_16850, HCQ42_001914, HEP30_011955, HEP34_001075, HHH44_003019, HI055_001485, HIE29_000052, HJQ60_005092, HLX92_03690, HLZ50_01830, HMV95_16370, HV109_14610, HV146_09250, HV209_29060, HVV39_00410, HVW04_05590, HVW43_06430, HVY77_15765, HVZ29_08715, HX086_07035, HX136_15650, I0E88_003516, I6H00_08540, I6H02_24530, IFB95_003696, IUC12_000121, J0541_003169, J5U05_002959, JNP96_10710, KV259_001392, KV317_003230, KV371_000953, KV406_001102, KV449_001342, KV455_003227, KV463_001049, KV469_000542, KV499_000679, KV500_001199, NCTC10082_00641, NCTC10089_03005, NCTC10090_00680, NCTC10418_04509, NCTC10764_02647, NCTC10767_05044, NCTC10865_03684, NCTC10974_03416, NCTC11126_00919, NCTC11181_00164, NCTC11341_04894, NCTC12950_03296, NCTC13148_05706, NCTC4450_04127, NCTC7922_00634, NCTC7927_03385, NCTC7928_00455, NCTC8009_05657, NCTC8179_02874, NCTC8333_03590, NCTC8621_03238, NCTC8622_05813, NCTC8959_00826, NCTC8960_00763, NCTC8985_01747, NCTC9044_04397, NCTC9045_03550, NCTC9073_01975, NCTC9075_04022, NCTC9077_03763, NCTC9081_03511, NCTC9117_03837, NCTC9706_00412, NCTC9962_06984, NEP60_22810, O5851_21660, RG28_16890, SAMEA3752557_01555, TUM18780_25170, WR15_23855
Production host: Escherichia coli (E. coli) / References: UniProt: C3TE02
#50: Protein 50S ribosomal protein L3 / Large ribosomal subunit protein uL3


Mass: 22277.535 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplC, b3320, JW3282 / Production host: Escherichia coli (E. coli) / References: UniProt: P60438
#51: Protein 50S ribosomal protein L33


Mass: 6388.631 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmG, rpmG_1, A5U30_004136, A6592_21955, A8499_000792, A8502_002345, A9X72_00400, AC065_17670, ACN81_07125, ACU57_07205, AM464_10960, APX88_07835, AW118_10740, AWP47_15985, B6R12_001909, B6R15_ ...Gene: rpmG, rpmG_1, A5U30_004136, A6592_21955, A8499_000792, A8502_002345, A9X72_00400, AC065_17670, ACN81_07125, ACU57_07205, AM464_10960, APX88_07835, AW118_10740, AWP47_15985, B6R12_001909, B6R15_003224, B6R17_003336, B6R31_002865, B6R48_003283, B6R87_003156, BANRA_04677, BB540_10150, BER14_16165, BF481_000671, BG944_002239, BGM66_003973, BHS81_21690, BJI68_21710, BJJ90_00390, BK292_09690, BK383_09650, BKL28_001549, BMT50_10565, BMT91_13845, BR158_003843, BTB68_002398, BTQ06_17850, BUO55_001749, BvCmsKKP061_03496, BvCmsSIP010_04504, BXT93_06220, BZL69_22230, C0P57_003843, C1Q91_001907, C2121_003322, C2M16_17175, C2R31_004081, C3F40_17705, C5N07_22255, C9E67_00890, C9Z68_15420, CA593_07900, CCS08_06865, CCU84_004237, CDL36_16035, CF22_002026, CG692_01875, CG704_10010, CG831_000991, CIG67_03795, CQ986_000108, CQB02_22645, CR538_00630, CR539_23800, CR628_001528, CRM83_20955, CTR35_001077, CV83915_02464, CWS33_14490, CX938_000998, CY655_23100, D3C88_33140, D3G36_09395, D4M65_14910, D4N09_11500, D6T60_21010, D9D43_20690, D9E34_16170, D9E49_10195, D9H94_14645, D9J03_19890, D9J61_14005, DD762_15060, DIV22_25130, DNQ45_17840, DNX30_21910, DS732_26145, DTL43_17805, DTL90_19180, DTM45_13255, DU321_24275, E0I52_14235, E4K51_19990, E5H86_15640, E6D34_14175, EA239_11595, EB534_RS18180, EC95NR1_03037, ECs4511, EIA08_13065, EIMP300_11190, EIZ93_22910, EN85_002943, EPS76_16410, EPS97_14120, ERS085406_02634, ERS139208_03027, EWK56_19235, ExPECSC038_00886, F3P10_10725, F7F11_22995, F7N46_06895, F9413_20155, F9461_04500, F9B07_09850, FA868_19515, FEJ01_05435, FFF58_21805, FGG80_07720, FHD44_20765, FHQ97_14415, FIJ20_14735, FJQ40_22750, FKO60_20795, FN993_001905, FOI11_017545, FOI11_05635, FPI65_22380, FPS11_15665, FTV92_18865, FTV93_19030, FV293_17885, FVB16_08575, FWK02_20790, FZN31_00850, FZU14_11310, G3V95_06555, G3W53_09755, G4A38_07645, G4A47_07035, G5603_14155, G9448_15395, GAI89_21025, GAJ12_25750, GAJ26_19905, GGB84_002816, GIB53_07680, GJ11_23260, GKF52_07040, GKF66_06090, GKF89_01305, GNW61_15010, GOP14_002925, GOP25_22240, GP711_14020, GP965_18420, GP975_06785, GQM04_18880, GQM13_19245, GQM17_16535, GQM21_20675, GQN34_13365, GQW07_04785, GRO95_05820, GRW05_17070, GRW24_04500, GRW56_13140, GRW57_14295, GSM54_05345, GSY44_19835, GUC01_19125, GUI33_04785, H0O53_07375, H0O72_08715, H0P11_12255, H5Z35_003529, H6Y26_001161, HCQ42_003479, HEP30_004320, HEP34_000581, HHH44_001955, HI055_001939, HIE29_002315, HJQ60_004388, HJY54_003834, HKA49_003561, HLQ92_16260, HLV18_18020, HLX92_21165, HLZ50_07765, HMV95_17960, HMW38_12345, HV109_00345, HV146_00330, HV209_24710, HVV39_10960, HVW04_15855, HVW43_16870, HVY77_00425, HVZ29_00305, HVZ30_00460, HVZ71_00385, HX136_00340, I6H00_19205, I6H02_13660, IFB95_001325, IUD45_003655, J0541_001519, J4S20_003173, J5U05_003670, J7T50_004317, JFD_02865, JFD_02951, JNP96_26980, KQ660_13480, NCTC10082_02483, NCTC10089_00164, NCTC10090_03179, NCTC10418_00180, NCTC10429_00616, NCTC10487_00103, NCTC10764_03481, NCTC10767_01151, NCTC10865_00201, NCTC10974_00127, NCTC11112_01599, NCTC11126_01709, NCTC11127_03609, NCTC11181_02394, NCTC11341_02337, NCTC12950_00092, NCTC13127_00380, NCTC4450_02132, NCTC7922_04520, NCTC7927_00189, NCTC7928_02970, NCTC8009_00971, NCTC8179_05536, NCTC8333_00121, NCTC8450_04550, NCTC8500_05792, NCTC8621_00171, NCTC8959_03599, NCTC8960_02723, NCTC8985_04527, NCTC9001_03782, NCTC9044_01835, NCTC9045_00169, NCTC9050_03266, NCTC9071_00140, NCTC9073_06389, NCTC9075_00237, NCTC9077_00137, NCTC9081_00502, NCTC9111_00516, NCTC9117_00296, NCTC9702_00106, NCTC9706_02375, NCTC9775_03637, NCTC9777_01519, NCTC9962_01072, NEP60_12970, OS110_19615, RG28_23350, SAMEA3472044_00655, SAMEA3472056_04704, SAMEA3752557_01144, SAMEA3753106_03495, TUM18780_00760, WR15_15810
Production host: Escherichia coli (E. coli) / References: UniProt: C3SME7
#53: Protein/peptide 50S ribosomal protein L34


Mass: 5397.463 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmH / Production host: Escherichia coli (E. coli) / References: UniProt: B7MGC4
#55: Protein 50S ribosomal protein L35 / Large ribosomal subunit protein bL35 / Ribosomal protein A


Mass: 7313.032 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmI, b1717, JW1707 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7Q1
#57: Protein/peptide 50S ribosomal protein L36


Mass: 4377.390 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmJ / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2L017
#62: Protein 50S ribosomal protein L16 / Large ribosomal subunit protein uL16


Mass: 15312.269 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplP, b3313, JW3275 / Production host: Escherichia coli (E. coli) / References: UniProt: P0ADY7
#66: Protein 50S ribosomal protein L20


Mass: 13528.024 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplT / Production host: Escherichia coli (E. coli) / References: UniProt: B7MAS6

+
Large ribosomal subunit protein ... , 14 types, 14 molecules 4Zbelnprstuwxy

#5: Protein Large ribosomal subunit protein bL25


Mass: 10713.465 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplY / Production host: Escherichia coli (E. coli) / References: UniProt: P68919
#40: Protein Large ribosomal subunit protein bL12


Mass: 12309.155 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rplL, A2J79_004435, A5U30_004587, ABE91_020830, ACU57_23195, AWP47_16720, B6R15_000402, B6R31_004824, BANRA_00090, BANRA_02961, BANRA_05058, BCB93_004499, BG944_004750, BGM66_002188, BK300_ ...Gene: rplL, A2J79_004435, A5U30_004587, ABE91_020830, ACU57_23195, AWP47_16720, B6R15_000402, B6R31_004824, BANRA_00090, BANRA_02961, BANRA_05058, BCB93_004499, BG944_004750, BGM66_002188, BK300_15065, BK383_27730, BKL28_004341, BMT50_08100, BMT91_03295, BRV02_004441, BTB68_004075, BTQ06_27275, BvCmsKKP061_02984, BXT93_25165, C0P57_003016, C1Q91_004814, C2M16_25120, C2R31_004868, C3F40_15470, C6669_07605, C719_004809, C7B02_25775, C9160_23390, C9194_21585, C9Z68_22025, CCS08_08130, CF22_004729, CG702_19645, CIG67_06960, CQ842_10390, CQ842_21780, CR538_24220, CTR35_004064, CWS33_24560, D3G36_22415, D4M65_19265, D4N09_21560, D9D43_22305, D9E49_24265, D9J61_19505, DD762_22290, DIV22_03735, DL968_14800, DNQ45_05175, DNX30_24155, DS732_01540, DTL43_13710, DU321_23435, E2863_04083, E4K51_22910, E5H86_23035, E6D34_20870, EAI46_11705, ECs4909, EIA08_23400, EIZ93_16325, EN85_004183, EPS76_13580, EPS97_19160, EWK56_25660, ExPECSC038_04329, EXX87_24990, F7F11_23825, F7N46_22870, F9413_20755, F9461_25100, F9B07_23660, FGAF848_44570, FIJ20_20100, FJQ40_20825, FKO60_24825, FOI11_015720, FOI11_24360, FPI65_24515, FPS11_24515, FTV93_16950, FV293_23420, FVB16_05300, FZU14_22845, G3V95_20440, G3W53_22325, G4A38_22930, G4A47_22790, G5603_24110, GAI89_24235, GAJ12_23545, GKF66_20535, GNW61_19020, GNZ05_25250, GOP25_23370, GP711_23605, GP944_20380, GP954_05265, GP965_06580, GP975_06490, GP979_07410, GQA06_03460, GQM04_07510, GQM21_14975, GQN24_22100, GRC73_22180, GRW05_10065, GRW24_04155, GTP92_22605, GUC01_23015, HEP30_021885, HEP34_005295, HHH44_004415, HI055_004309, HIE29_005266, HJQ60_004918, HKA49_004954, HLX92_23675, HLZ39_21235, HLZ50_21720, HMV95_21220, HV109_22430, HV209_21200, HVV39_13130, HVW04_13840, HVW43_14990, HVY77_24380, HX136_23645, I6H02_15710, IFC14_004534, IH772_15225, J0541_004715, J5U05_003961, J8F57_004531, JNA68_24645, JNP96_01270, NCTC10082_02083, NCTC10279_03474, NCTC10429_00090, NCTC10764_02974, NCTC10865_05897, NCTC10974_05353, NCTC11112_02279, NCTC11126_05452, NCTC11181_01967, NCTC11341_02772, NCTC7922_04997, NCTC7927_05251, NCTC7928_03628, NCTC8009_08435, NCTC8179_05098, NCTC8333_05560, NCTC8500_05327, NCTC8603_04078, NCTC8621_04874, NCTC8622_01626, NCTC8960_02342, NCTC8985_04032, NCTC9001_04471, NCTC9044_02302, NCTC9045_05544, NCTC9075_06429, NCTC9077_05938, NCTC9117_05906, NCTC9702_05641, NCTC9706_02005, NCTC9962_06032, NOI85_25205, NY836_12435, OFN31_22165, OGM49_00570, P6223_004405, PWL68_004987, Q2V20_25675, Q2V64_25165, QDW62_24500, QO046_17555, R8G00_17440, R8O40_005074, RZR61_19910, SAMEA3472044_04876, SAMEA3752557_04841, V9Z47_24995, WR15_01480
Production host: Escherichia coli (E. coli) / References: UniProt: C3SIB2
#42: Protein Large ribosomal subunit protein bL27


Mass: 9146.540 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpmA / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7M0
#45: Protein Large ribosomal subunit protein uL29


Mass: 7286.464 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_003599, ...Gene: rpmC, A5U30_004546, A8502_004029, ACN81_25490, ACU57_20990, AW118_24870, AWP47_12675, B6R15_004075, B6R31_004974, BANRA_01943, BANRA_04322, BE932_15505, BER14_25160, BG944_001127, BGM66_003599, BGZ_02596, BGZ_04119, BJI68_08705, BK292_20475, BK383_24675, BKL28_004549, BTB68_004362, BTQ06_10660, BvCmsKKP061_03024, BvCmsSIP010_02679, BXT93_05505, C0P57_002662, C1Q91_004910, C2121_004168, C2M16_25435, C2R31_004627, C3F40_19750, C9Z68_22605, CF22_004855, CG704_20900, CG831_003643, CIG67_10705, CTR35_003624, CV83915_02786, D3G36_23080, D4M65_20625, D4N09_20225, D9D43_22580, D9E49_05210, D9J61_16705, DD762_23600, DIV22_15205, DNQ45_03810, DNX30_25425, DS732_24315, DTL43_20945, DU321_12055, E4K51_21545, E5H86_24470, E6D34_21705, EAI46_07530, ECs4177, EIA08_24010, EIMP300_17750, EIZ93_12415, EN85_004355, EPS76_07160, EPS97_20040, EWK56_25050, ExPECSC038_03646, F7F11_22670, F7N46_23955, F9413_21180, F9461_25600, F9B07_24630, FGAF848_25910, FIJ20_21140, FJQ40_18360, FKO60_25690, FOI11_019205, FOI11_03975, FORC44_0486, FPI65_20360, FPS11_25375, FTV93_20845, FVB16_04925, FWK02_06325, FZU14_21875, G3V95_19900, G3W53_21010, G4A38_21565, G4A47_20835, G5603_24640, GAI89_24775, GAJ12_24665, GKF66_21600, GNW61_16685, GOP25_22760, GP711_23360, GP954_00890, GP965_04350, GP975_01240, GP979_01950, GQA06_03895, GQE86_20135, GQM04_10620, GQM13_25255, GQM21_11285, GQN34_23320, GQW07_21495, GRC73_21695, GRO95_20760, GRW05_09115, GRW24_04700, GRW56_00350, GRW57_03485, GUC01_21235, H0O72_19470, HEP30_018690, HEP34_004760, HHH44_004525, HI055_004150, HIE29_005197, HJQ60_005001, HLX92_10020, HLZ50_22125, HMV95_19660, HV109_02135, HV209_14660, HVV39_09320, HVW04_17480, HVW43_18615, HVY77_02120, I6H00_20870, I6H02_12045, IFB95_004816, J0541_004407, J5U05_004077, JNP96_25360, KV499_004411, NCTC10082_02829, NCTC10089_00492, NCTC10418_00681, NCTC10429_01084, NCTC10764_03853, NCTC10767_01506, NCTC10865_00649, NCTC10974_00539, NCTC11126_02760, NCTC11181_02728, NCTC11341_01899, NCTC7922_04052, NCTC7927_00541, NCTC7928_02527, NCTC8009_01501, NCTC8179_05932, NCTC8333_00479, NCTC8500_00324, NCTC8621_00498, NCTC8622_00516, NCTC8959_03271, NCTC8960_03060, NCTC8985_04972, NCTC9001_04244, NCTC9044_01368, NCTC9045_00555, NCTC9075_00714, NCTC9077_00594, NCTC9081_00907, NCTC9117_00746, NCTC9702_00527, NCTC9706_02727, NCTC9962_06787, NEP60_26345, OGM49_22385, QDW62_02140, RZR61_12945, SAMEA3472112_00683, SAMEA3752557_01962, WR15_19660
Production host: Escherichia coli (E. coli) / References: UniProt: C3SQY2
#52: Protein Large ribosomal subunit protein uL4 / 50S ribosomal protein L4


Mass: 22121.566 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplD, eryA, b3319, JW3281 / Production host: Escherichia coli (E. coli) / References: UniProt: P60723
#54: Protein Large ribosomal subunit protein uL5 / 50S ribosomal protein L5


Mass: 20333.611 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplE, b3308, JW3270 / Production host: Escherichia coli (E. coli) / References: UniProt: P62399
#56: Protein Large ribosomal subunit protein uL6 / 50S ribosomal protein L6


Mass: 18932.791 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplF, b3305, JW3267 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG55
#58: Protein Large ribosomal subunit protein bL9 / 50S ribosomal protein L9


Mass: 15789.020 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplI, b4203, JW4161 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7R1
#59: Protein Large ribosomal subunit protein uL13 / 50S ribosomal protein L13


Mass: 16050.606 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplM, b3231, JW3200 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AA10
#60: Protein Large ribosomal subunit protein uL14 / 50S ribosomal protein L14


Mass: 13565.067 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplN, b3310, JW3272 / Production host: Escherichia coli (E. coli) / References: UniProt: P0ADY3
#61: Protein Large ribosomal subunit protein uL15 / 50S ribosomal protein L15


Mass: 15008.471 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplO, b3301, JW3263 / Production host: Escherichia coli (E. coli) / References: UniProt: P02413
#63: Protein Large ribosomal subunit protein bL17 / 50S ribosomal protein L17


Mass: 14393.657 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplQ, b3294, JW3256 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG44
#64: Protein Large ribosomal subunit protein uL18 / 50S ribosomal protein L18


Mass: 12794.668 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplR, b3304, JW3266 / Production host: Escherichia coli (E. coli) / References: UniProt: P0C018
#65: Protein Large ribosomal subunit protein bL19


Mass: 13159.278 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rplS / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7K8

+
DNA chain , 2 types, 2 molecules 56

#6: DNA chain NT DNA


Mass: 11042.110 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others)
#7: DNA chain T DNA


Mass: 10956.012 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others)

+
RNA chain , 5 types, 6 molecules 7ABDad

#8: RNA chain mRNA with 60 nt long spacer


Mass: 23934.291 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others)
#10: RNA chain E-site and P-site tRNA (fMet)


Mass: 24496.617 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 1870104878
#18: RNA chain 16S rRNA


Mass: 499690.031 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 1732209707
#41: RNA chain 23S rRNA


Mass: 941635.438 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 937521852
#44: RNA chain 5S rRNA


Mass: 38790.090 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli) / References: GenBank: 1273279017

+
DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules AAACADAEAF

#11: Protein DNA-directed RNA polymerase subunit beta / RNAP subunit beta / RNA polymerase subunit beta / Transcriptase subunit beta


Mass: 150820.875 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: rpoB, groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950
Production host: Escherichia coli (E. coli) / References: UniProt: P0A8V2, DNA-directed RNA polymerase
#13: Protein DNA-directed RNA polymerase subunit alpha / RNAP subunit alpha / RNA polymerase subunit alpha / Transcriptase subunit alpha


Mass: 36558.680 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoA, pez, phs, sez, b3295, JW3257 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7Z4, DNA-directed RNA polymerase
#14: Protein DNA-directed RNA polymerase subunit beta' / RNAP subunit beta' / RNA polymerase subunit beta' / Transcriptase subunit beta'


Mass: 155366.781 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoC, tabB, b3988, JW3951 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A8T7, DNA-directed RNA polymerase
#15: Protein DNA-directed RNA polymerase subunit omega / RNAP omega subunit / RNA polymerase omega subunit / Transcriptase subunit omega


Mass: 10249.547 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpoZ, b3649, JW3624 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A800, DNA-directed RNA polymerase

+
Transcription termination/antitermination protein ... , 2 types, 2 molecules ABAG

#12: Protein Transcription termination/antitermination protein NusG


Mass: 20560.523 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: nusG, Z5555, ECs4905 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AFG1
#16: Protein Transcription termination/antitermination protein NusA


Mass: 54932.684 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: nusA / Production host: Escherichia coli (E. coli) / References: UniProt: P0AFF8

+
Small ribosomal subunit protein ... , 18 types, 18 molecules CEFHIJKLMNOPSTUVWX

#17: Protein Small ribosomal subunit protein bS18


Mass: 9005.472 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsR / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2KXL3
#19: Protein Small ribosomal subunit protein bS20


Mass: 9708.464 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsT / Production host: Escherichia coli (E. coli) / References: UniProt: C3TRH7
#20: Protein Small ribosomal subunit protein bS21


Mass: 8524.039 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsU / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0E2L2J1
#22: Protein Small ribosomal subunit protein bS1 / 30S ribosomal protein S1


Mass: 61238.887 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsA, Z1257, ECs0994 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AG69
#23: Protein Small ribosomal subunit protein uS3


Mass: 26031.316 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsC / Production host: Escherichia coli (E. coli) / References: UniProt: B7MCS9
#24: Protein Small ribosomal subunit protein uS4


Mass: 23514.199 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsD, ramA, b3296, JW3258 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7V8
#25: Protein Small ribosomal subunit protein uS5


Mass: 17629.398 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsE, spc, b3303, JW3265 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7W1
#26: Protein Small ribosomal subunit protein bS6


Mass: 15727.512 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsF, b4200, JW4158 / Production host: Escherichia coli (E. coli) / References: UniProt: P02358
#27: Protein Small ribosomal subunit protein uS7


Mass: 20055.156 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsG, b3341, JW3303 / Production host: Escherichia coli (E. coli) / References: UniProt: P02359
#28: Protein Small ribosomal subunit protein uS8


Mass: 14146.557 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsH / Production host: Escherichia coli (E. coli) / References: UniProt: C3SR12
#29: Protein Small ribosomal subunit protein uS9


Mass: 14886.270 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsI / Production host: Escherichia coli (E. coli) / References: UniProt: C3SRY2
#30: Protein Small ribosomal subunit protein uS10


Mass: 11755.597 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsJ / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQT7
#33: Protein Small ribosomal subunit protein uS14


Mass: 11606.560 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsN, NCTC9073_00385 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2X1PQW4
#34: Protein Small ribosomal subunit protein uS15


Mass: 10290.816 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsO / Production host: Escherichia coli (E. coli) / References: UniProt: C3SSQ7
#35: Protein Small ribosomal subunit protein bS16


Mass: 9207.572 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsP / Production host: Escherichia coli (E. coli) / References: UniProt: C3SYP2
#36: Protein Small ribosomal subunit protein uS17


Mass: 9724.491 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsQ / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQY7
#37: Protein Small ribosomal subunit protein uS19


Mass: 10455.355 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsS / Production host: Escherichia coli (E. coli) / References: UniProt: C3SQW2
#38: Protein Small ribosomal subunit protein uS13


Mass: 13128.467 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsM, G711_03878 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A7U9IV78

+
30S ribosomal protein ... , 3 types, 3 molecules GQR

#21: Protein 30S ribosomal protein S2


Mass: 26781.670 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsB / Production host: Escherichia coli (E. coli) / References: UniProt: C3TPN2
#31: Protein 30S ribosomal protein S11


Mass: 13870.975 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsK / Production host: Escherichia coli (E. coli) / References: UniProt: B7MCR3
#32: Protein 30S ribosomal protein S12


Mass: 13768.157 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: rpsL, strA, b3342, JW3304 / Production host: Escherichia coli (E. coli) / References: UniProt: P0A7S3

+
Non-polymers , 2 types, 3 molecules

#67: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg
#68: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn

+
Details

Has ligand of interestN
Has protein modificationY

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Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: Escherichia coli transcription-translation loosely coupled complex (TTC-LC) containing mRNA with a 60 nt long spacer, NusG, NusA, and fMet-tRNAs in E-site and P-site
Type: COMPLEX / Entity ID: #1-#66 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
MicroscopyModel: FEI TALOS ARCTICA
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1250 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 4.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 32821 / Symmetry type: POINT

+
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