regulation of cilium movement / axonemal dynein complex assembly / epithelial cilium movement involved in determination of left/right asymmetry / inner dynein arm assembly / sperm axoneme assembly / cytoskeletal motor regulator activity / cilium-dependent cell motility / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / regulation of Arp2/3 complex-mediated actin nucleation ...regulation of cilium movement / axonemal dynein complex assembly / epithelial cilium movement involved in determination of left/right asymmetry / inner dynein arm assembly / sperm axoneme assembly / cytoskeletal motor regulator activity / cilium-dependent cell motility / sperm principal piece / regulation of cilium beat frequency involved in ciliary motility / regulation of Arp2/3 complex-mediated actin nucleation / axoneme assembly / axonemal dynein complex / flagellated sperm motility / actomyosin structure organization / locomotion / motile cilium / positive regulation of cell motility / myosin II complex / myosin heavy chain binding / axoneme / mitotic cytokinesis / cilium assembly / sperm flagellum / ciliary basal body / post-embryonic development / cell projection / cell motility / cilium / small GTPase binding / cell migration / lamellipodium / microtubule binding / microtubule / cytoskeleton / calcium ion binding / Golgi apparatus / ATP hydrolysis activity / ATP binding / plasma membrane / cytoplasm 類似検索 - 分子機能
Coiled-coil domain-containing protein 153 / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / Domain of unknown function DUF4201 / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / Dynein regulatory complex protein 9 / Domain of unknown function (DUF4201) ...Coiled-coil domain-containing protein 153 / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / Domain of unknown function DUF4201 / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / Dynein regulatory complex protein 9 / Domain of unknown function (DUF4201) / Cilia- and flagella-associated protein 91 / Growth arrest-specific protein 8 domain / Growth arrest-specific protein 8 / Dynein regulatory complex protein 1/2, N-terminal / Dynein regulatory complex protein / Growth-arrest specific micro-tubule binding / Sperm tail / BRE1 E3 ubiquitin ligase / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / CAP superfamily / EF-hand domain / Leucine rich repeat 4 / Leucine Rich repeats (2 copies) / Leucine rich repeat, ribonuclease inhibitor type / Leucine Rich repeat / Leucine-rich repeat, SDS22-like subfamily / IQ motif profile. / IQ motif, EF-hand binding site / Leucine-rich repeat profile. / Leucine-rich repeat / Papain-like cysteine peptidase superfamily / ATPase family associated with various cellular activities (AAA) / Leucine-rich repeat domain superfamily / ATPase, AAA-type, core / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Dynein regulatory complex protein 10 / Growth-arrest-specific microtubule-binding protein / AAA family ATPase CDC48 subfamily protein / Cilia- and flagella-associated protein 91 / Cilia- and flagella-associated protein 184 / Calmodulin 7-2 / Dynein regulatory complex subunit 3 / Coiled-coil lobo-like protein, putative / EF hand protein / Dynein regulatory complex protein 1/2 N-terminal domain-containing protein ...Dynein regulatory complex protein 10 / Growth-arrest-specific microtubule-binding protein / AAA family ATPase CDC48 subfamily protein / Cilia- and flagella-associated protein 91 / Cilia- and flagella-associated protein 184 / Calmodulin 7-2 / Dynein regulatory complex subunit 3 / Coiled-coil lobo-like protein, putative / EF hand protein / Dynein regulatory complex protein 1/2 N-terminal domain-containing protein / Cilia- and flagella-associated protein 263 / Cilia- and flagella-associated protein 20 / Dynein regulatory complex protein 12 / Coiled-coil protein, putative / WD40 domain protein / Flagella associated protein / Dynein regulatory complex protein 9 / Growth-arrest-specific microtubule-binding protein / Flagellar associated protein / Kinase domain protein / Dynein regulatory complex subunit 2 / WD40 domain protein / EF-hand calcium-binding domain protein 類似検索 - 構成要素
Natural Sciences and Engineering Research Council (NSERC, Canada)
カナダ
引用
ジャーナル: Nat Commun / 年: 2023 タイトル: Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism. 著者: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward M ...著者: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui / 要旨: Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein ...Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity.