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- EMDB-41189: Baseplate of Nexin-dynein regulatory complex from Tetrahymena the... -

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Basic information

Entry
Database: EMDB / ID: EMD-41189
TitleBaseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila
Map dataThe baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia
Sample
  • Organelle or cellular component: Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila
    • Protein or peptide: DUF4201 domain-containing protein
    • Protein or peptide: Flagellar associated protein
    • Protein or peptide: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
    • Protein or peptide: Coiled-coil protein, putative
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Cilia- and flagella-associated protein 91
    • Protein or peptide: Flagella associated protein
    • Protein or peptide: Coiled-coil protein, putative
    • Protein or peptide: CFAP20
Keywordscomplex / Cilia / axoneme / nexin-dynein regulatory complex / PROTEIN BINDING
Function / homology
Function and homology information


regulation of cilium movement / axonemal dynein complex assembly / inner dynein arm assembly / axonemal dynein complex / cilium-dependent cell motility / cilium movement / axoneme assembly / motile cilium / axoneme / cilium assembly ...regulation of cilium movement / axonemal dynein complex assembly / inner dynein arm assembly / axonemal dynein complex / cilium-dependent cell motility / cilium movement / axoneme assembly / motile cilium / axoneme / cilium assembly / cell projection / cell motility / small GTPase binding / microtubule binding / microtubule / cytoskeleton / ciliary basal body / Golgi apparatus / cytoplasm
Similarity search - Function
: / : / CFAP184 CAP-like domain / CFAP184 CBM-like domain / Coiled-coil domain-containing protein 39 / Domain of unknown function DUF4201 / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 ...: / : / CFAP184 CAP-like domain / CFAP184 CBM-like domain / Coiled-coil domain-containing protein 39 / Domain of unknown function DUF4201 / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / : / CCDC113/CCDC96, coiled-coil / Cilia- and flagella-associated protein 91 / Growth arrest-specific protein 8 domain / Growth arrest-specific protein 8 / Dynein regulatory complex protein 1/2, N-terminal / Dynein regulatory complex protein / Growth-arrest specific micro-tubule binding / Sperm tail / BRE1 E3 ubiquitin ligase / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / CAP superfamily
Similarity search - Domain/homology
Growth-arrest-specific microtubule-binding protein / Cilia- and flagella-associated protein 91 / Cilia- and flagella-associated protein 184 / Dynein regulatory complex protein 1/2 N-terminal domain-containing protein / Cilia- and flagella-associated protein 263 / Transcription factor iib protein, putative / Coiled-coil protein, putative / Flagella associated protein / Growth-arrest-specific microtubule-binding protein / Dynein regulatory complex subunit 2
Similarity search - Component
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsGhanaeian AG / Black CS / Yang SK / Bui KH
Funding support Canada, 2 items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR)PJT-156354 Canada
Natural Sciences and Engineering Research Council (NSERC, Canada)RGPIN-2022-04774 Canada
CitationJournal: Nat Commun / Year: 2023
Title: Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism.
Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward ...Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui /
Abstract: Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein ...Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity.
History
DepositionJul 6, 2023-
Header (metadata) releaseSep 20, 2023-
Map releaseSep 20, 2023-
UpdateSep 27, 2023-
Current statusSep 27, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_41189.png

Downloads & links

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Map

FileDownload / File: emd_41189.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.37 Å/pix.
x 512 pix.
= 701.44 Å		1.37 Å/pix.
x 512 pix.
= 701.44 Å		1.37 Å/pix.
x 512 pix.
= 701.44 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.37 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.016
Minimum - Maximum-0.04395528 - 0.08868455
Average (Standard dev.)-0.0005772059 (±0.0052444194)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 701.44 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_41189_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: The half map of baseplate domain of N-DRC...

Fileemd_41189_half_map_1.map
AnnotationThe half map of baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: The half map of baseplate domain of N-DRC...

Fileemd_41189_half_map_2.map
AnnotationThe half map of baseplate domain of N-DRC on doublet microtubule in Tetrahymena thermophila cilia
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Baseplate of Nexin-dynein regulatory complex from Tetrahymena the...

EntireName: Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila
Components
  • Organelle or cellular component: Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila
    • Protein or peptide: DUF4201 domain-containing protein
    • Protein or peptide: Flagellar associated protein
    • Protein or peptide: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
    • Protein or peptide: Coiled-coil protein, putative
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Cilia- and flagella-associated protein 91
    • Protein or peptide: Flagella associated protein
    • Protein or peptide: Coiled-coil protein, putative
    • Protein or peptide: CFAP20

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Supramolecule #1: Baseplate of Nexin-dynein regulatory complex from Tetrahymena the...

SupramoleculeName: Baseplate of Nexin-dynein regulatory complex from Tetrahymena thermophila
type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #8-#9, #1-#7, #10
Details: Single Particle Analysis of baseplate domain of Nexin-Dynein regulatory complex of Tetrahymena thermophila
Source (natural)Organism: Tetrahymena thermophila (eukaryote)

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Macromolecule #1: Dynein regulatory complex protein 1/2 N-terminal domain-containin...

MacromoleculeName: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
type: protein_or_peptide / ID: 1 / Details: The baseplate domain of the DRC1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 98.840125 KDa
SequenceString: MNPSQTQKGK IQIQVSRGSI DLLVRTMYED INNIKLKNER FQKKKRAEDE RRRRERYAEI QQEAIASGKK NAALEMKWAE LKELDECEE LNREMQDQQQ TFQQIIEGKE NLRKEFEEEL KRKDDEYVKM LKEQSNDIKD MISKMRSQFY KIRDANMQEL E DIERHFEQ ...String:
MNPSQTQKGK IQIQVSRGSI DLLVRTMYED INNIKLKNER FQKKKRAEDE RRRRERYAEI QQEAIASGKK NAALEMKWAE LKELDECEE LNREMQDQQQ TFQQIIEGKE NLRKEFEEEL KRKDDEYVKM LKEQSNDIKD MISKMRSQFY KIRDANMQEL E DIERHFEQ ERSNLLNEFK AKIQATFKKH LDLEEQYVKE HAKTEEEQTK NIEDLRIKGA KHYAELKITM ETEIQDLEKC FE DMKALYQ LITEKLDYSL KVLQEKFHEN NSMCDELKRK ENNFKNRLKQ LTKDYKQYDK KFKLENKNLT QEYKRIIRQF KEL QKKFKH FEKADLDKYS EIQKMNEAEV KELKDKIIKC NITIHIQQLG MQWIPPQSEE EILAQQKLQL EQGGKAEEEE EREF EFCIS EIKIGEICNI ILEEADFLID DKLREELEGS AEPEQIFQRL DCIKKCLYID SEDEMNLFFR ELITKCRVKS AEEIE EEAR KQLLLLGLIK EAEENENPEG EEVQQQEGAQ QKKKEEGNKK DDPKGKEGAK DSKENANPQG QNKSNQDERK KKKDGQ DDH AGQGQGQYDS NQGGMGENQE EGENAEGQEN LEGEIEKEID LENTQINLNE VVKFLQNWQA NKDKRKEKLE KESSKKA VK QETEREKKER IAREGKKYWE KLTQVLPERT FRIWNVNFFK FSFEKQILLN QINKLRFWTD LCPNIMNFFW TDKNQSKK P VNCITKMKNQ RIFQINTYKL IMNLLSALQD SLIQIKDKDD FIQTKISTNL FTFHNFQILL QIFIVNQSFL FIRLCQSYS KKIILLIKNI NQKLFNLSNF KNQFSF

UniProtKB: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein

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Macromolecule #2: Coiled-coil protein, putative

MacromoleculeName: Coiled-coil protein, putative / type: protein_or_peptide / ID: 2 / Details: The baseplate domain of the DRC2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 60.596348 KDa
SequenceString: MALLGKTLKG RSTRPGGIPQ KRNIKWRQLA KNQEEFDQLK QLAKMKREGL KARIKDEQKV VTFNKKKLIT YWRKIMRIAK TEQLKNEID IYSQNNQREL DSKEAFIQML DKNLDEAEDQ FQIALRNHLI HIENLMQLQE ARMRGLAEEF NRDVNILETE F DLEREEMV ...String:
MALLGKTLKG RSTRPGGIPQ KRNIKWRQLA KNQEEFDQLK QLAKMKREGL KARIKDEQKV VTFNKKKLIT YWRKIMRIAK TEQLKNEID IYSQNNQREL DSKEAFIQML DKNLDEAEDQ FQIALRNHLI HIENLMQLQE ARMRGLAEEF NRDVNILETE F DLEREEMV KTHKTQLKEL EDMIETVKEE DKKKTEEAQN EFSQFKEETK NKNLEETNVM KIILETKQTK YYTELEQMNS KF QSDTSNK VKDHQFYHAH NKNRKQEIDR YLRTISSKKA KIDLMKLKIL QHCKEFNARN SALKKEKENI SRNYHELKLK MQK FREEES RRLKELSNNS RNAVLKLREY CALGEKILKT AELCRRLETE KEKVLPFYES SVDEDQIPEQ LKNEFEHLKK EDAE EYAYL NNFYKRYNKV LLDKLAIEKQ KENLQRDNQL LKSLLKQYLD GISLNDDVLK NENNPLLVVN HKFNLGKMPV EKIEN KTVI EGVFEVRNTS HQLQGQRAPP FQ

UniProtKB: Dynein regulatory complex subunit 2

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Macromolecule #3: Growth-arrest-specific microtubule-binding protein

MacromoleculeName: Growth-arrest-specific microtubule-binding protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 56.190844 KDa
SequenceString: MSKAAKAKKN VPVVSKLEAD ARKAAEGVND NESEEFKKAM RKEARALVEQ FNEEKKLLAF YQQERQKINY NWIIAKKELE DKKSELINK EREIQDLQEN HFMTLNVYKQ KIKHLLFQNQ DQQSELKKDV EVTLKQLEDQ HRIKSRELKT DVRSLKVTKK E QEISQQDY ...String:
MSKAAKAKKN VPVVSKLEAD ARKAAEGVND NESEEFKKAM RKEARALVEQ FNEEKKLLAF YQQERQKINY NWIIAKKELE DKKSELINK EREIQDLQEN HFMTLNVYKQ KIKHLLFQNQ DQQSELKKDV EVTLKQLEDQ HRIKSRELKT DVRSLKVTKK E QEISQQDY LFALKSEHDK QMTLMRQDYE RQVNDIKRKY DLKMQNLTKE MEEARAAMIK QLEDNKNQKI AEIIKEHTQK YN DIKNYYS EITATNLDYR KTLKNEIKEL QTKDEEYKKT LQQTEKGYKE LNEPLQALGI EIIQLKKQDE EQEAIIKEKE ELK QKIDNQ ERLFRKLEYE YEVKLQQFQY LERERNALYA KFNQTVFEIH QKSGLENLIL EKKVTNLRED LEIKDLQIHQ VLTA ANIDP NSVGSINKSL EEVESLKNEL ISELQAQLKQ IRKAHSHMVK AYEGKLSEFV IPVEELGFDP LVPTNTD

UniProtKB: Growth-arrest-specific microtubule-binding protein

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Macromolecule #4: Growth-arrest-specific microtubule-binding protein

MacromoleculeName: Growth-arrest-specific microtubule-binding protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 55.393203 KDa
SequenceString: MPPKKAKGKK KKEEEPDDEY KSMTGADLTQ TLEKLKERVN EMRTNRNYIQ MDRDMVENFY HNTLKEISEV KTKISNKETE AEEKESKHR IDVKVFLQKV KHLEYEQEKS NLNIEDDGKK AKEKEDAYFE DITKNMKQLK TQLKSEYLEK EKANIQQVQE E KKDHQSLL ...String:
MPPKKAKGKK KKEEEPDDEY KSMTGADLTQ TLEKLKERVN EMRTNRNYIQ MDRDMVENFY HNTLKEISEV KTKISNKETE AEEKESKHR IDVKVFLQKV KHLEYEQEKS NLNIEDDGKK AKEKEDAYFE DITKNMKQLK TQLKSEYLEK EKANIQQVQE E KKDHQSLL KIQQKKFDEL INNLIIKYEE RLAKLKEDLE LKLKVEIHEL EERKNLHINE LMNNHEKAFA ELKKYYNDIT AE NLNLIKA HKEKIAQIYA NIQLNTKNVA DNQAKNEQLK EPLAKHREIR NKLKEDLKQF AKHKMSLQNL KSKAITLKDK ITK LERDGK DLDEKYEKVV REKQELEKKF EDITQEVKKN ADLNNNVLSN RLQILLKEYN NKEEELRTII DNAGLDHNLH EQLK QRVQQ SIEAKNTLIK NLKYSIHHAT KAYNDAIRVY EAKLVEFGIP IEELGFQPLE TITSSMPAGL VSS

UniProtKB: Growth-arrest-specific microtubule-binding protein

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Macromolecule #5: Cilia- and flagella-associated protein 91

MacromoleculeName: Cilia- and flagella-associated protein 91 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 76.154328 KDa
SequenceString: MATTTNILHD VVRDQSMVNY VSNRDRPLYF KRPLVPQMTD IPLHISRPPV DQQVDYQTMQ MQQNATIVEA PTKDAFVQTD YRESETQTD PYTPKCFVRD GDHPEVMELK DYKYGKGLPA SIEELEQIEL NREKVWFENS LPPISDEASF NLRRKLMEEQ E LREWSKKE ...String:
MATTTNILHD VVRDQSMVNY VSNRDRPLYF KRPLVPQMTD IPLHISRPPV DQQVDYQTMQ MQQNATIVEA PTKDAFVQTD YRESETQTD PYTPKCFVRD GDHPEVMELK DYKYGKGLPA SIEELEQIEL NREKVWFENS LPPISDEASF NLRRKLMEEQ E LREWSKKE NEIKKFQNEK LYLLQQALIE REKEVEDKSQ ERIEEIRQQK TEHKNRQIAK IQRKKIKIDR KMTKSRKMQG KE TLKRDII EDYANFASRV YAGITHEGLS LDKIANKYEV QPLALGNYEM LQALHEGIRP REFETRVNLK KEIKEIEKNY TRL ENYHRG ELKKAQDEIN GVHEQSKAQQ KQEGNNFSYR NFENKIRPAT PTWKYDTDFN ISPSLITEIQ EYRGPNGVQL MQAA DKREE AVLLLQRLLR GRTTQNIMYE GKKKRTALIE ELLTVAQIEN LEEDKAEEVL MQQHEEKVKN AVLESIQGEV IAETM DELS KELLRIKQER KIQQMVEIAE KDRRIREIEE AGKRQAEEIL RDREDVLHNQ LIRVHQGTVD TYLDWLMSNA LEQSSA RQA TIMTNLRKAK FNLPLEDFER KYNNNQTLIK DLVHSFLIPN VQRSKLKKQI QLEEKRFSEA AKRSLQQTIT RASNKHA NV QN

UniProtKB: Cilia- and flagella-associated protein 91

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Macromolecule #6: Flagella associated protein

MacromoleculeName: Flagella associated protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 114.163266 KDa
SequenceString: MDQGEFIQDD NDQDYDQQEG DDENNDYMED PQQDNNYNQN GDNLDDQGQD DDGGQNNLQQ PSVPPEDDED DDDDFPEYAN EQNKRLNEI IKKKRKLIKD ISAKIEEKSD RTKVLQEHLK NVEQELLHTQ ALIDAKNKEI ETEDHMKQIA ERQSGRIQSE L KILEKRSI ...String:
MDQGEFIQDD NDQDYDQQEG DDENNDYMED PQQDNNYNQN GDNLDDQGQD DDGGQNNLQQ PSVPPEDDED DDDDFPEYAN EQNKRLNEI IKKKRKLIKD ISAKIEEKSD RTKVLQEHLK NVEQELLHTQ ALIDAKNKEI ETEDHMKQIA ERQSGRIQSE L KILEKRSI EQQEKLNDVQ NQIFRGNEKM DQYKLEMNWN QEELEQWALA ARQKEEDNLT LEKYKRADEV KIKELNLAIE KL TAEVGRK QNELEKEITE TQAAQIELDK TAEEFKRQHE ERHKLFLQWQ EVTEIISKRD LAIREEGENF ARIKIEIKSN KDA LEERKR ILKEAKEENK RIQMANELME RQNIQQISDN KHIEEQLAEK KADVEILKNQ VSAFASDLSS KKNRIAILSQ ELLA KKQRL NAAQKKYQAH QVKLKNEEIM AKQYENDRSY AEEKYRKNEK EKKELEKEIR TQKENLFKHT QELFKHRERE ANLYG EIQG NMAACRNLQS HITKLNQEFQ RQQELLYNAE YQIQLMERRV ARAKGERTLE EKKDLENEIM NAERELGGVT GQNKEL IES LKNLDDEIRT VKKKLAYVEE ENTKYSSLIE ELILENDMTY QDLNKIIKQK EEVLVQHDTM KLEIKKINES LNGATEK VF NLENQIYQLE MSMQEREKEI EVHKDVLMTE HKAAEEERHK IAVELAERKN KVKNLKIKYE SLVQKNKASN GEVESVHE H SQAYYVIKAA QEREELQRKG DELNAKILKN EKELKALDNT LNHLKNRNSN YRDKFLNKGV TTQHREEAEG LEEQCRAAS QNLFKKRDEL QKLQKEQEED TRRYTEIKNK LERLYEQHQS LDQEISKYQK DIDQQGDKLQ RAQNSLQRNY QLAVNKNQNF INPKNPHMI QVKLDNQNNL YKTLLQGLYS LQQDIPELSS VVDEILKEQR IQNNKAPSSI DINSKRSSQS GRSQRSQRSN I SNQ

UniProtKB: Flagella associated protein

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Macromolecule #7: Coiled-coil protein, putative

MacromoleculeName: Coiled-coil protein, putative / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 105.196055 KDa
SequenceString: MSNQQGPEDN NLEDDMAYLP ADHPLLAKLQ IDLTKQLTDE HERVDQKLIE IDANLKKLEK TKEDIGVRLY SVQQQLAENQ MNFEQAHEN YNWVQKLRIE AEQKLKTESE VYDAKKKELE ELRKKYLKAQ DELSKLTRTL YQINEFNQQM KGQIINTKTN T YRAEENVV ...String:
MSNQQGPEDN NLEDDMAYLP ADHPLLAKLQ IDLTKQLTDE HERVDQKLIE IDANLKKLEK TKEDIGVRLY SVQQQLAENQ MNFEQAHEN YNWVQKLRIE AEQKLKTESE VYDAKKKELE ELRKKYLKAQ DELSKLTRTL YQINEFNQQM KGQIINTKTN T YRAEENVV NLEAQKKKQD LLIDTMNEEI KRQTEQKTIL TAQLISQKEE TEQAKQILKE AHLEMQKIIA SKKNLLERWQ KS LMTMQRM DNALQAIKEA LKGQQELNLQ IGTELNGVNA EIRKETEIQE SLEGKNKKFD YEKDYLQKKY NELQEEKSKL EAQ INLLTQ SLRQTETEAG RAEIDKRNIE DQMNLIETNI MKLHTETKKL WEDLVHQKSE HTTIEKTATN LNKQANQISI EIED KSVEL ENLLNEIARV KIDQLNTLSQ IEVLENKRRE VIKEREEKEI TVATYEVQIR QGHDLNEKKQ HEVGRLNREH DKLSS VQSD MSRGPLEAKR NNLIRKTQEL GKENDLMQRE WIKKQTLLVT QNNRLNKIEE DVSQLKTKQT ILEQKKLRLN NNYRIY EKD IREIQNALKN LRNEMNKLND AIYRNKEKQQ KLDNENFNIK SEFVEKLKEL EKESVKLEVE IDRLKEEKAD LLAEIVE SE RQILLWERKI QLEKEMQDAL DPTVGQTEIQ ELKREIHRME LRLDDLRKKQ EAIIAEMERA VYKRETIQLK YMNKDKTF S NSNSMSQKSS SISAASDNSA QITKKIAQLK TTLNQTTRNA EQMEKAIKNK KIELDDLNAQ IEGNNDNLQK LESDCYNKN IELTKHKLER STNILSISCM QNKAKKLEDL VAGKARLSVP EATLMTKYEE LRDKNQEIKE ALQKLCDDAP QYVEVLNYLI DLNVGDDDE DQEQ

UniProtKB: Coiled-coil protein, putative

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Macromolecule #8: DUF4201 domain-containing protein

MacromoleculeName: DUF4201 domain-containing protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 94.540883 KDa
SequenceString: MASEDGEMPS QFEGIDPEQL TESQMQQYMM EMQRQGLLDS NMEGGQYEEG YEEGQEGEGY GEEYGDQDYD GNQNEYDSQQ DSQQQGHDQ VNEMHQDRYD RRVNSEISGN YEADDETLRK IRRDLLDNIN LERRHRDLQP VYIDLTTNNI SQYYSEYLVN N EHNQDFYE ...String:
MASEDGEMPS QFEGIDPEQL TESQMQQYMM EMQRQGLLDS NMEGGQYEEG YEEGQEGEGY GEEYGDQDYD GNQNEYDSQQ DSQQQGHDQ VNEMHQDRYD RRVNSEISGN YEADDETLRK IRRDLLDNIN LERRHRDLQP VYIDLTTNNI SQYYSEYLVN N EHNQDFYE NAKVRYNNLG ECELCHITAK FEPDADITKE YIYDYFMEIG YLFLESEEEK RIILNPANNH IGIGVFFDEI QI VVVLILS EKVLCIQKIS QPEQNKIEIR GKMLDENFGI YAIRIMNVDD QKKDIKGVGP EFIEYTRSTQ EWMASFELEL YNQ DRMAIE YYTRVSPDSI PYKKKQSKNE KLTYKHLQLR LRTPFQIYPD PKYAAEDEKE RIRKEQEILA HEEQERKERE ENDA KRLKQ SRKDYGDGQY DDDEHGQDDF NSQSDISDKD KHHDSMHAEQ EQNQQAAQQQ QDTISNKEIR QELEMAITEA QRQHD EFML QNHKLQEEIK LLKNKNDGFV DRSNETAMNE HKYLNTLAHV HQIRLDLKQT QTRYNQMSQE LQKKLEQKQK KCNEIK YAF LELKREVAKK AANSRTDKPI PEQQINEWEK AELQKSKELQ ELRLQILRLR NAYVKNQKIL KKKEELAEGL HLIDFEQ LK IENQTLNEKI EERNEELHKL KKKNTTTIQI LTHTREKLGF VQGENGELNS QNVRKDQELD DMRKQLTQQK KTKDKLRS V NLTLKQQTGI VNSEELGQDY RDLRTRVSKL EEEKKRLEQK LRSMHEAIKT ANQISTQNMQ SQNNSLKKPY QPY

UniProtKB: Cilia- and flagella-associated protein 184

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Macromolecule #9: Flagellar associated protein

MacromoleculeName: Flagellar associated protein / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 42.931082 KDa
SequenceString: MNQDKHPEIT DEEQELITVE ELSQKLELLY KDMEQIRREN LLFEAYLARN RKEIAKEDEV SEDKKGKGKK KDKNVDKKSL LLTNEEKFE IAQQEQDALK KQIDDGRIKS DQILETLRAI LEETDMAITE IRKDAFDFQR EILVGGENSR TGKIEAEKII K FFEEKERQ ...String:
MNQDKHPEIT DEEQELITVE ELSQKLELLY KDMEQIRREN LLFEAYLARN RKEIAKEDEV SEDKKGKGKK KDKNVDKKSL LLTNEEKFE IAQQEQDALK KQIDDGRIKS DQILETLRAI LEETDMAITE IRKDAFDFQR EILVGGENSR TGKIEAEKII K FFEEKERQ KDALIAKYSS KRTNLERQIL KTNNQIQKKE EMGDDLKFID FYQLQIENKK YVKEIDDKNK KLLALKISTN RI SQTLKDE KQNLKQELDK GKEYASQMSE RKKKISKIDA QIKSVKKVTS KLEKDRKIYD KQKEIFVQDN QDDVPQIMKY VQY KSKEQQ LLYAIQNLER KIEIAELAYK KANRILQSSQ QFQQK

UniProtKB: Cilia- and flagella-associated protein 263

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Macromolecule #10: CFAP20

MacromoleculeName: CFAP20 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 22.976535 KDa
SequenceString: MFKNTFQSGF LSILYSIGSK PLQIWDKSIR NGHIKRITDQ DILSSVLEIM GTNVSTNYIT APADPKETLG IKLPFLVMII KNLKKYYTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDEGW NQIQFNLSDF TRRAYATNYI ETLRVQIHAN C RIRRIYFS ...String:
MFKNTFQSGF LSILYSIGSK PLQIWDKSIR NGHIKRITDQ DILSSVLEIM GTNVSTNYIT APADPKETLG IKLPFLVMII KNLKKYYTF EVQVLDDKNV RRRFRASNYQ STTRVKPFIC TMPMRLDEGW NQIQFNLSDF TRRAYATNYI ETLRVQIHAN C RIRRIYFS DRLYSEEELP PEFKLFLPIQ GQNKTNV

UniProtKB: Transcription factor iib protein, putative

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

8537

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: OTHER / Number images used: 211502
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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