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- EMDB-41251: Linker domain of Nexin-dynein regulatory complex from Tetrahymena... -

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Basic information

Entry
Database: EMDB / ID: EMD-41251
TitleLinker domain of Nexin-dynein regulatory complex from Tetrahymena thermophila
Map dataPrimary map
Sample
  • Cell: Linker domain of Nexin-dynein regulatory complex from Tetrahymena thermophila
    • Protein or peptide: x 14 types
  • Protein or peptide: x 1 types
Keywordsnexin-dynein regulatory complex / cilia / axoneme / dynein / STRUCTURAL PROTEIN
Function / homology
Function and homology information


regulation of cilium movement / axonemal dynein complex assembly / axonemal dynein complex / cilium-dependent cell motility / regulation of Arp2/3 complex-mediated actin nucleation / cilium organization / motile cilium / axoneme / cilium assembly / ciliary basal body ...regulation of cilium movement / axonemal dynein complex assembly / axonemal dynein complex / cilium-dependent cell motility / regulation of Arp2/3 complex-mediated actin nucleation / cilium organization / motile cilium / axoneme / cilium assembly / ciliary basal body / cell motility / cilium / small GTPase binding / cell migration / lamellipodium / microtubule binding / microtubule / cytoskeleton / calcium ion binding / Golgi apparatus / ATP hydrolysis activity / ATP binding / plasma membrane / cytoplasm
Similarity search - Function
Coiled-coil domain-containing protein 153 / : / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / : / Domain of unknown function DUF4201 / Dynein regulatory complex protein 9 / : / Domain of unknown function (DUF4201) / Growth arrest-specific protein 8 domain ...Coiled-coil domain-containing protein 153 / : / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / : / Domain of unknown function DUF4201 / Dynein regulatory complex protein 9 / : / Domain of unknown function (DUF4201) / Growth arrest-specific protein 8 domain / Growth arrest-specific protein 8 / Dynein regulatory complex protein 1/2, N-terminal / Dynein regulatory complex protein / : / Growth-arrest specific micro-tubule binding / Sperm tail / : / CAP superfamily / : / EF-hand domain / Leucine rich repeat 4 / Leucine Rich repeats (2 copies) / Leucine rich repeat, ribonuclease inhibitor type / Leucine Rich repeat / Leucine-rich repeat, SDS22-like subfamily / IQ motif profile. / Leucine-rich repeat profile. / Leucine-rich repeat / Papain-like cysteine peptidase superfamily / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / Leucine-rich repeat domain superfamily / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Dynein regulatory complex protein 10 / Growth-arrest-specific microtubule-binding protein / AAA family ATPase CDC48 subfamily protein / Cilia- and flagella-associated protein 184 / Calmodulin 7-2 / Dynein regulatory complex subunit 3 / Coiled-coil lobo-like protein, putative / Dynein regulatory complex protein 1/2 N-terminal domain-containing protein / Dynein regulatory complex protein 12 / Dynein regulatory complex protein 9 ...Dynein regulatory complex protein 10 / Growth-arrest-specific microtubule-binding protein / AAA family ATPase CDC48 subfamily protein / Cilia- and flagella-associated protein 184 / Calmodulin 7-2 / Dynein regulatory complex subunit 3 / Coiled-coil lobo-like protein, putative / Dynein regulatory complex protein 1/2 N-terminal domain-containing protein / Dynein regulatory complex protein 12 / Dynein regulatory complex protein 9 / Growth-arrest-specific microtubule-binding protein / Flagellar associated protein / Kinase domain protein / Dynein regulatory complex subunit 2 / EF-hand calcium-binding domain protein
Similarity search - Component
Biological speciesTetrahymena thermophila (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.4 Å
AuthorsGhanaeian AG / Bui KH
Funding support Canada, 2 items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR) Canada
Natural Sciences and Engineering Research Council (NSERC, Canada) Canada
CitationJournal: Nat Commun / Year: 2023
Title: Integrated modeling of the Nexin-dynein regulatory complex reveals its regulatory mechanism.
Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward ...Authors: Avrin Ghanaeian / Sumita Majhi / Caitlyn L McCafferty / Babak Nami / Corbin S Black / Shun Kai Yang / Thibault Legal / Ophelia Papoulas / Martyna Janowska / Melissa Valente-Paterno / Edward M Marcotte / Dorota Wloga / Khanh Huy Bui /
Abstract: Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein ...Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity.
History
DepositionJul 14, 2023-
Header (metadata) releaseSep 20, 2023-
Map releaseSep 20, 2023-
UpdateOct 23, 2024-
Current statusOct 23, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_41251.jpg

emd_41251.png

Downloads & links

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Map

FileDownload / File: emd_41251.map.gz / Format: CCP4 / Size: 43.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPrimary map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.74 Å/pix.
x 208 pix.
= 569.92 Å		2.74 Å/pix.
x 211 pix.
= 578.14 Å		2.74 Å/pix.
x 258 pix.
= 706.92 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

generated in cubic-lattice coordinate

Voxel sizeX=Y=Z: 2.74 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy EMDB: 0.0217
Minimum - Maximum-0.09863708 - 0.17370053
Average (Standard dev.)0.00037829683 (±0.006335534)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-43-1-13
Dimensions211258208
Spacing258211208
CellA: 706.92 Å / B: 578.14 Å / C: 569.92 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_41251_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: half map 1

Fileemd_41251_half_map_1.map
Annotationhalf map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: half map 2

Fileemd_41251_half_map_2.map
Annotationhalf map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : Linker domain of Nexin-dynein regulatory complex from Tetrahymena...

EntireName: Linker domain of Nexin-dynein regulatory complex from Tetrahymena thermophila
Components
  • Cell: Linker domain of Nexin-dynein regulatory complex from Tetrahymena thermophila
    • Protein or peptide: Calmodulin 7-2
    • Protein or peptide: LRRC48 protein
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Growth-arrest-specific microtubule-binding protein
    • Protein or peptide: Flagellar associated protein
    • Protein or peptide: Kinase domain protein
    • Protein or peptide: EF-hand calcium-binding domain protein
    • Protein or peptide: Dynein regulatory complex protein 9
    • Protein or peptide: Dynein regulatory complex protein 10
    • Protein or peptide: AAA family ATPase CDC48 subfamily protein
    • Protein or peptide: DUF4201 domain-containing protein
    • Protein or peptide: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
    • Protein or peptide: Coiled-coil protein, putative
    • Protein or peptide: Coiled-coil domain-containing protein 153
  • Protein or peptide: Coiled-coil lobo-like protein, putative

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Supramolecule #1: Linker domain of Nexin-dynein regulatory complex from Tetrahymena...

SupramoleculeName: Linker domain of Nexin-dynein regulatory complex from Tetrahymena thermophila
type: cell / ID: 1 / Parent: 0 / Macromolecule list: #14, #3-#7, #9-#13, #1-#2, #15
Source (natural)Organism: Tetrahymena thermophila (eukaryote)

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Macromolecule #1: Dynein regulatory complex protein 1/2 N-terminal domain-containin...

MacromoleculeName: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 98.840125 KDa
SequenceString: MNPSQTQKGK IQIQVSRGSI DLLVRTMYED INNIKLKNER FQKKKRAEDE RRRRERYAEI QQEAIASGKK NAALEMKWAE LKELDECEE LNREMQDQQQ TFQQIIEGKE NLRKEFEEEL KRKDDEYVKM LKEQSNDIKD MISKMRSQFY KIRDANMQEL E DIERHFEQ ...String:
MNPSQTQKGK IQIQVSRGSI DLLVRTMYED INNIKLKNER FQKKKRAEDE RRRRERYAEI QQEAIASGKK NAALEMKWAE LKELDECEE LNREMQDQQQ TFQQIIEGKE NLRKEFEEEL KRKDDEYVKM LKEQSNDIKD MISKMRSQFY KIRDANMQEL E DIERHFEQ ERSNLLNEFK AKIQATFKKH LDLEEQYVKE HAKTEEEQTK NIEDLRIKGA KHYAELKITM ETEIQDLEKC FE DMKALYQ LITEKLDYSL KVLQEKFHEN NSMCDELKRK ENNFKNRLKQ LTKDYKQYDK KFKLENKNLT QEYKRIIRQF KEL QKKFKH FEKADLDKYS EIQKMNEAEV KELKDKIIKC NITIHIQQLG MQWIPPQSEE EILAQQKLQL EQGGKAEEEE EREF EFCIS EIKIGEICNI ILEEADFLID DKLREELEGS AEPEQIFQRL DCIKKCLYID SEDEMNLFFR ELITKCRVKS AEEIE EEAR KQLLLLGLIK EAEENENPEG EEVQQQEGAQ QKKKEEGNKK DDPKGKEGAK DSKENANPQG QNKSNQDERK KKKDGQ DDH AGQGQGQYDS NQGGMGENQE EGENAEGQEN LEGEIEKEID LENTQINLNE VVKFLQNWQA NKDKRKEKLE KESSKKA VK QETEREKKER IAREGKKYWE KLTQVLPERT FRIWNVNFFK FSFEKQILLN QINKLRFWTD LCPNIMNFFW TDKNQSKK P VNCITKMKNQ RIFQINTYKL IMNLLSALQD SLIQIKDKDD FIQTKISTNL FTFHNFQILL QIFIVNQSFL FIRLCQSYS KKIILLIKNI NQKLFNLSNF KNQFSF

UniProtKB: Dynein regulatory complex protein 1/2 N-terminal domain-containing protein

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Macromolecule #2: Coiled-coil protein, putative

MacromoleculeName: Coiled-coil protein, putative / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 60.596348 KDa
SequenceString: MALLGKTLKG RSTRPGGIPQ KRNIKWRQLA KNQEEFDQLK QLAKMKREGL KARIKDEQKV VTFNKKKLIT YWRKIMRIAK TEQLKNEID IYSQNNQREL DSKEAFIQML DKNLDEAEDQ FQIALRNHLI HIENLMQLQE ARMRGLAEEF NRDVNILETE F DLEREEMV ...String:
MALLGKTLKG RSTRPGGIPQ KRNIKWRQLA KNQEEFDQLK QLAKMKREGL KARIKDEQKV VTFNKKKLIT YWRKIMRIAK TEQLKNEID IYSQNNQREL DSKEAFIQML DKNLDEAEDQ FQIALRNHLI HIENLMQLQE ARMRGLAEEF NRDVNILETE F DLEREEMV KTHKTQLKEL EDMIETVKEE DKKKTEEAQN EFSQFKEETK NKNLEETNVM KIILETKQTK YYTELEQMNS KF QSDTSNK VKDHQFYHAH NKNRKQEIDR YLRTISSKKA KIDLMKLKIL QHCKEFNARN SALKKEKENI SRNYHELKLK MQK FREEES RRLKELSNNS RNAVLKLREY CALGEKILKT AELCRRLETE KEKVLPFYES SVDEDQIPEQ LKNEFEHLKK EDAE EYAYL NNFYKRYNKV LLDKLAIEKQ KENLQRDNQL LKSLLKQYLD GISLNDDVLK NENNPLLVVN HKFNLGKMPV EKIEN KTVI EGVFEVRNTS HQLQGQRAPP FQ

UniProtKB: Dynein regulatory complex subunit 2

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Macromolecule #3: LRRC48 protein

MacromoleculeName: LRRC48 protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 68.009617 KDa
SequenceString: MSNYISNYVQ STIVTKTVQN KDKKTGKNSV DPRVINEQLI LDAVKQFNQE NNKITTENII LQQLRQLQLS FKNILKIQHL QGLERLEKL QLDNNIIEKI ENIDHLVNLK WLDLSFNCIK KIEGLDKLKE LTDLSLFNNY IEKIEGLHNN TKLNVFSIGN N RLKSYEEI ...String:
MSNYISNYVQ STIVTKTVQN KDKKTGKNSV DPRVINEQLI LDAVKQFNQE NNKITTENII LQQLRQLQLS FKNILKIQHL QGLERLEKL QLDNNIIEKI ENIDHLVNLK WLDLSFNCIK KIEGLDKLKE LTDLSLFNNY IEKIEGLHNN TKLNVFSIGN N RLKSYEEI TLYFGYKPRG EEGTNDRPEF KRLQVLNVSG NPFTKDKENE YKNHIICAIP NLKYLDYVFI DEGDRQLIRQ DE QISNSYN FTTTDYYQQM QAQEQKEEMD RQTLKKKKDA RMDILDNLKD EYLKIEDLQR VKIIKPNQIN EEIEKYCGKI NDH VVEMQA AVIQKHQLIL QEMSKTEVSY KEQEALHEKE TLQILKEFEH EKKVEFRNWE KEAANGDAKK EESRLKTLLQ KTTS LKNKL MDIEIQLVEE LEAIFTDYDT KKKETAWKDI EEIIVLGQKK IEEENVKFYS NLNVIKNKEE ANYNSTQQQQ LEEEN NEED DEKASLLENK EQLAQMIGNI KETLIDSHTK IILSAQMELQ KDLAEYAKQQ KNKMYERNRK NISQIIAQID AYQTEI NEK LKQGDDESDH EN

UniProtKB: Dynein regulatory complex subunit 3

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Macromolecule #4: Growth-arrest-specific microtubule-binding protein

MacromoleculeName: Growth-arrest-specific microtubule-binding protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 56.190844 KDa
SequenceString: MSKAAKAKKN VPVVSKLEAD ARKAAEGVND NESEEFKKAM RKEARALVEQ FNEEKKLLAF YQQERQKINY NWIIAKKELE DKKSELINK EREIQDLQEN HFMTLNVYKQ KIKHLLFQNQ DQQSELKKDV EVTLKQLEDQ HRIKSRELKT DVRSLKVTKK E QEISQQDY ...String:
MSKAAKAKKN VPVVSKLEAD ARKAAEGVND NESEEFKKAM RKEARALVEQ FNEEKKLLAF YQQERQKINY NWIIAKKELE DKKSELINK EREIQDLQEN HFMTLNVYKQ KIKHLLFQNQ DQQSELKKDV EVTLKQLEDQ HRIKSRELKT DVRSLKVTKK E QEISQQDY LFALKSEHDK QMTLMRQDYE RQVNDIKRKY DLKMQNLTKE MEEARAAMIK QLEDNKNQKI AEIIKEHTQK YN DIKNYYS EITATNLDYR KTLKNEIKEL QTKDEEYKKT LQQTEKGYKE LNEPLQALGI EIIQLKKQDE EQEAIIKEKE ELK QKIDNQ ERLFRKLEYE YEVKLQQFQY LERERNALYA KFNQTVFEIH QKSGLENLIL EKKVTNLRED LEIKDLQIHQ VLTA ANIDP NSVGSINKSL EEVESLKNEL ISELQAQLKQ IRKAHSHMVK AYEGKLSEFV IPVEELGFDP LVPTNTD

UniProtKB: Growth-arrest-specific microtubule-binding protein

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Macromolecule #5: Growth-arrest-specific microtubule-binding protein

MacromoleculeName: Growth-arrest-specific microtubule-binding protein / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 55.393203 KDa
SequenceString: MPPKKAKGKK KKEEEPDDEY KSMTGADLTQ TLEKLKERVN EMRTNRNYIQ MDRDMVENFY HNTLKEISEV KTKISNKETE AEEKESKHR IDVKVFLQKV KHLEYEQEKS NLNIEDDGKK AKEKEDAYFE DITKNMKQLK TQLKSEYLEK EKANIQQVQE E KKDHQSLL ...String:
MPPKKAKGKK KKEEEPDDEY KSMTGADLTQ TLEKLKERVN EMRTNRNYIQ MDRDMVENFY HNTLKEISEV KTKISNKETE AEEKESKHR IDVKVFLQKV KHLEYEQEKS NLNIEDDGKK AKEKEDAYFE DITKNMKQLK TQLKSEYLEK EKANIQQVQE E KKDHQSLL KIQQKKFDEL INNLIIKYEE RLAKLKEDLE LKLKVEIHEL EERKNLHINE LMNNHEKAFA ELKKYYNDIT AE NLNLIKA HKEKIAQIYA NIQLNTKNVA DNQAKNEQLK EPLAKHREIR NKLKEDLKQF AKHKMSLQNL KSKAITLKDK ITK LERDGK DLDEKYEKVV REKQELEKKF EDITQEVKKN ADLNNNVLSN RLQILLKEYN NKEEELRTII DNAGLDHNLH EQLK QRVQQ SIEAKNTLIK NLKYSIHHAT KAYNDAIRVY EAKLVEFGIP IEELGFQPLE TITSSMPAGL VSS

UniProtKB: Growth-arrest-specific microtubule-binding protein

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Macromolecule #6: Flagellar associated protein

MacromoleculeName: Flagellar associated protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 52.938457 KDa
SequenceString: MLAKKLALAR SQGKPDPFKK DEFPLLQHIA VEVVSQNYIL YPDLKGVPEN VRDTIISKIT TDLDILTMAP NIDQESFWQR ACKNRWEKS QKSINIEEHG RSWKVAYLER YMEEFLTNIQ NADDPNKKQI LQAELKAAAS WIHTLNLQNI NQNIDIDFIC K YLPLLTSL ...String:
MLAKKLALAR SQGKPDPFKK DEFPLLQHIA VEVVSQNYIL YPDLKGVPEN VRDTIISKIT TDLDILTMAP NIDQESFWQR ACKNRWEKS QKSINIEEHG RSWKVAYLER YMEEFLTNIQ NADDPNKKQI LQAELKAAAS WIHTLNLQNI NQNIDIDFIC K YLPLLTSL TLTYGTKYSG MDYNKQSVGM KLSEAANLGE AIKNCYSLLS LNISANMIDD DLLRFIMAGV NMNISLIELN LS HNKIEDQ GARRIAKFLM RNEILLYLNL GNNLIGYEGS RYLAQALKVN KNLQSLNLKL NNLGDKAGKK LFQDLMLNKT LLE LDVSGN QFEFETALKL SDYVADSMCG LKVLNIANND FNDSCYENLK TGFTKNYSLA KLDLRGNKFS KTQEEKEQEL KDPF RMFNL SQTEKELTQI IIRHDLTAQK IQFFSESDLD KIKQLKELQG AKQEPEKVIQ QDQNKE

UniProtKB: Flagellar associated protein

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Macromolecule #7: Kinase domain protein

MacromoleculeName: Kinase domain protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 39.706977 KDa
SequenceString: MGDKKGKKKG GGEEDESTLQ LSRQYKKKCE LNGINPSKLF KEKLDFAVEE DENIEKIHLW EELGPVGVRS IMDSLTEIPY LHTKSIRLW KVKAQDEGTR TICNYMEKSK TIEYLDLMDN QVGSLGCEFL GRVLHPQIEI PLLKLKLDHN EFGTEGLMQL A AGLCMNSV ...String:
MGDKKGKKKG GGEEDESTLQ LSRQYKKKCE LNGINPSKLF KEKLDFAVEE DENIEKIHLW EELGPVGVRS IMDSLTEIPY LHTKSIRLW KVKAQDEGTR TICNYMEKSK TIEYLDLMDN QVGSLGCEFL GRVLHPQIEI PLLKLKLDHN EFGTEGLMQL A AGLCMNSV LEKLSLNYCA ITAEGSKYIQ QILSFVNTNL KKLKMKGNLL KNEGVHQLFR AFKVNKTLEK VDLSDNQFDT TL GDDQDPE ANPNFFETNK DAIEHNKQII ENICEVLKAA DTLLYYDFRF NNISKTDAKA IIDCLEANKN IYYMELSEHI PKD LIERKK GLMKKRKPKK KKKKKAKKK

UniProtKB: Kinase domain protein

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Macromolecule #8: Coiled-coil lobo-like protein, putative

MacromoleculeName: Coiled-coil lobo-like protein, putative / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 101.225328 KDa
SequenceString: MDHNEGGYSH HQIEGQEGQQ QQAPVGGGGE DIFQVLENIT QVQNEKLKRC QEFKEQENIP RQYIENSPKE ELVLEHVIQF KRQFQYQLY YEDKRDLFLY PKNECDVYKF ICTTIRPTKL GFLELYDYQQ CSKYLSEFVQ YEELDPPNEF PLVIPSPTNV A VWQKGDCF ...String:
MDHNEGGYSH HQIEGQEGQQ QQAPVGGGGE DIFQVLENIT QVQNEKLKRC QEFKEQENIP RQYIENSPKE ELVLEHVIQF KRQFQYQLY YEDKRDLFLY PKNECDVYKF ICTTIRPTKL GFLELYDYQQ CSKYLSEFVQ YEELDPPNEF PLVIPSPTNV A VWQKGDCF DMSILLCSLL IGVGYDAYCV YGKAPREITT RNESLMECLF LEKGKYIEEN DKKPKQTLEQ NENALLKKPI AH SKWDQTQ ETKAREAEEE KRRIALTIND DEPDELLEDP YQGQRVHCWV LLRSGKRDVQ QNIFIEPSTG RMYSPSTCPY ECV DAVFNN LNFWINMKPE CEVKNLNFEE MDSSLNWEYV MLDTLLFNKG NGNDEEGNEN IDDPLNKQAQ RDPEQEQLQD IAQL LDMPP PWPPKIYIDK EKFLMGTPLG EGTVYYNKVK VDNYAPYSQP DGLVQKITIF QDYKRLKVKE YRYFYKHRSD KLSIR RRYP FEFKTIEEYE PQKYENKTIP QALQQWRQVI QIDRHLRIIK YYHNRNHDGL IERVEQIGEK TMMFYQNRDD RVIYRS IRF DNKRAPSSQN NDKVFQDNHV GDVQITKMTQ KFEKNPHYPA NEQIQKMVID LIKDKVIVYY HMNDGEITPI VKIYPRD SM HGFAKLNEPG GDNKIDDPLV QLENTKMVNL EKDCLNQLKQ QEKIARDDEK TIREGEIRLE KTLYDKARDR FKESSKKN D DDLAKDAAAS DYLYPYLEKR KLLGVSEINA QVAMEIKNEV MQKLKERLLS RAEIIQRRLE EQRQVLEQKE AQVAKRQEI DPKQEEEIRD INFKIDILEQ RALRFESMAL QKYEEMDNRL NKDARLAALH RK

UniProtKB: Coiled-coil lobo-like protein, putative

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Macromolecule #9: EF-hand calcium-binding domain protein

MacromoleculeName: EF-hand calcium-binding domain protein / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 22.062066 KDa
SequenceString:
MYDPNTSQTE KQKQEEFLKL KIQEAFNLFV KDKKGIVDKR EIPYIMRYLG QFPSEAQVRD AILPEIEEDE PSEFIKYSKF EPYMLKVLK EREYEPDDPE ALLAAFKLLD QEGKGYIEID MMKTFLEKQG IEFREQETKS FIEFATNKDP NATVIYYEDY I SRLQAFTD KHIESVMKGY NNFQVKK

UniProtKB: EF-hand calcium-binding domain protein

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Macromolecule #10: Dynein regulatory complex protein 9

MacromoleculeName: Dynein regulatory complex protein 9 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 44.990227 KDa
SequenceString: MNQIDAHKLT IILRDATERL TFLDTINRQD DLSSELAGYE ISKLLKKQKN LENQYADLVQ LRTSLTGIQN KKNLIETQTK IVDVAQNLK ESTKKLCRLF KENPDLESDA LKVKKDRIQF MAVIEQLIQM VQNNSLTKFQ SMTTLELEDQ DRLRKLIFRE K ELYQEIKK ...String:
MNQIDAHKLT IILRDATERL TFLDTINRQD DLSSELAGYE ISKLLKKQKN LENQYADLVQ LRTSLTGIQN KKNLIETQTK IVDVAQNLK ESTKKLCRLF KENPDLESDA LKVKKDRIQF MAVIEQLIQM VQNNSLTKFQ SMTTLELEDQ DRLRKLIFRE K ELYQEIKK LQFDRNQENK EYDNEQKDTN LKIQNLKERL LYKKARAQLK NQYKEKEARA EEGTQQRMYE FEQKQLKEKI NN LKQKTDT ENMVHEQLRN FLIEKEDQIK QKSDEWAKKV ETCREQLQEE IDRLTVEKEK KQEELRELRE RDQKEQEEKD RRE RQELEE ADRKQQEELD QIRLDNAIKL IQIEYQEWKD AGGGKKKRKK GKKKKK

UniProtKB: Dynein regulatory complex protein 9

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Macromolecule #11: Dynein regulatory complex protein 10

MacromoleculeName: Dynein regulatory complex protein 10 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 51.315023 KDa
SequenceString: MQNLSKMQSR LHPRPEGEGG GGGGRTKEKG KSLQETHKEM LKNIESTNIG KPTIVEAQRI LKIIDNLSTN LTIFIYLDSE LISKFLDVA KHSKLSKDLV SKLSQRCLDL LKSQAEIEAK FKPYASLLDQ SNKEAEDVEE EKMIDAERLR MQLENNFKDL V RHLRNSPE ...String:
MQNLSKMQSR LHPRPEGEGG GGGGRTKEKG KSLQETHKEM LKNIESTNIG KPTIVEAQRI LKIIDNLSTN LTIFIYLDSE LISKFLDVA KHSKLSKDLV SKLSQRCLDL LKSQAEIEAK FKPYASLLDQ SNKEAEDVEE EKMIDAERLR MQLENNFKDL V RHLRNSPE DFEIIKSMKT NVNPDLNDLV HCIHCQKVIM LKKLSTASEE DENHKAQLRD LEEKIEELEK KKNKIQEDLT KL KEHRQKF SKEKKEELEK LKNEIAEVKA EKEKKASQLK NELDNRLKQL ERDHLAKKDK LDKELQKLED DFAKLKQDHA NDE TKLKTN KRQQQNSLAD NIESYDALMK DQHQKKQEIE EELAKIIEEV DTLKQLFKEI DEEKQREREL EEEFRLKKEQ WEIQ ERRKK EAARCILHFL MARKEKSKKK KKKKGKKKK

UniProtKB: Dynein regulatory complex protein 10

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Macromolecule #12: AAA family ATPase CDC48 subfamily protein

MacromoleculeName: AAA family ATPase CDC48 subfamily protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 101.144125 KDa
SequenceString: MSNMMYNMKW QEAINDLMEQ VTLEYLPLEQ NEQQLGMKYK RDSSEWFHFW ATLYIKYIDI YKKLEDCYDQ LVHPQKRVLL KEMLENVIV RLCETKSQVV KYNTQYDATY KSDYPNLDEL VMDLKLTPDC LDIPIPRYFR EEDKKRLDER NQILDALLLE Y TDTKEPQE ...String:
MSNMMYNMKW QEAINDLMEQ VTLEYLPLEQ NEQQLGMKYK RDSSEWFHFW ATLYIKYIDI YKKLEDCYDQ LVHPQKRVLL KEMLENVIV RLCETKSQVV KYNTQYDATY KSDYPNLDEL VMDLKLTPDC LDIPIPRYFR EEDKKRLDER NQILDALLLE Y TDTKEPQE EQYEDQNTLQ ADMDTAIRII QRYERGRQGI ERANLAKILK KEEEKKLERQ KKLAEGTEIG EETEKDDAAL VI KKYWRGY KSRKLVQDIR EEELLFLGMK KVVEDPTLPE SQYKQAQNKR QKMKYIQEEH EQEFNDEIEN LKRLVKGNEG PDI LDKMRA ERREWIMREL EKNEFKEAPQ DPSEFYNQQV MDPEQQAAEA AKAAEEAKKK GAAKKDDKKK KGKPSELDEF LENN KPTGP SPIVLKLQEQ IEKHSGEWSK RDETNNFEQK HETELAKMLI KPVVEEQIKQ QVDEMIAEEL DIVRLRYDIK KKKQK KPPR PRNKGKNKKK FPGDSSNKGR DPKDILAGLV EKRVAKKLIP ASLMDLKGEQ NVLGKIQEIQ ADENLKKTEA LTDAKL KKA QLEEPSTKMP DPSIAQIRQI IAEYIAFPLG SKYAKEKLDK MNYFFFMGPR GSGKTLAVRA LAHECNAIVL DISPSNI DG QYTDKKQIDG MINSAFKVAK EFQPAIIYCE DFEYIFGQAK KKKSQVNPLF AKMKKPLMDF KKGKFFEPED RVVFIGST N RPWDCSQKEI KSFFDKKIYF PFPNYGTRML LFKTFLEQKK VPLPDNFPIN TIAHITEGWS AGSFKMAIDR VFTERRLQK INEEQIKLSE FIGPLSNVPF TSKEEFKEFK KFNQVVTGLQ ANYEAKKAPA DDQKGDKNKK KK

UniProtKB: AAA family ATPase CDC48 subfamily protein

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Macromolecule #13: DUF4201 domain-containing protein

MacromoleculeName: DUF4201 domain-containing protein / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 94.540883 KDa
SequenceString: MASEDGEMPS QFEGIDPEQL TESQMQQYMM EMQRQGLLDS NMEGGQYEEG YEEGQEGEGY GEEYGDQDYD GNQNEYDSQQ DSQQQGHDQ VNEMHQDRYD RRVNSEISGN YEADDETLRK IRRDLLDNIN LERRHRDLQP VYIDLTTNNI SQYYSEYLVN N EHNQDFYE ...String:
MASEDGEMPS QFEGIDPEQL TESQMQQYMM EMQRQGLLDS NMEGGQYEEG YEEGQEGEGY GEEYGDQDYD GNQNEYDSQQ DSQQQGHDQ VNEMHQDRYD RRVNSEISGN YEADDETLRK IRRDLLDNIN LERRHRDLQP VYIDLTTNNI SQYYSEYLVN N EHNQDFYE NAKVRYNNLG ECELCHITAK FEPDADITKE YIYDYFMEIG YLFLESEEEK RIILNPANNH IGIGVFFDEI QI VVVLILS EKVLCIQKIS QPEQNKIEIR GKMLDENFGI YAIRIMNVDD QKKDIKGVGP EFIEYTRSTQ EWMASFELEL YNQ DRMAIE YYTRVSPDSI PYKKKQSKNE KLTYKHLQLR LRTPFQIYPD PKYAAEDEKE RIRKEQEILA HEEQERKERE ENDA KRLKQ SRKDYGDGQY DDDEHGQDDF NSQSDISDKD KHHDSMHAEQ EQNQQAAQQQ QDTISNKEIR QELEMAITEA QRQHD EFML QNHKLQEEIK LLKNKNDGFV DRSNETAMNE HKYLNTLAHV HQIRLDLKQT QTRYNQMSQE LQKKLEQKQK KCNEIK YAF LELKREVAKK AANSRTDKPI PEQQINEWEK AELQKSKELQ ELRLQILRLR NAYVKNQKIL KKKEELAEGL HLIDFEQ LK IENQTLNEKI EERNEELHKL KKKNTTTIQI LTHTREKLGF VQGENGELNS QNVRKDQELD DMRKQLTQQK KTKDKLRS V NLTLKQQTGI VNSEELGQDY RDLRTRVSKL EEEKKRLEQK LRSMHEAIKT ANQISTQNMQ SQNNSLKKPY QPY

UniProtKB: Cilia- and flagella-associated protein 184

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Macromolecule #14: Calmodulin 7-2

MacromoleculeName: Calmodulin 7-2 / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 23.862807 KDa
SequenceString: MDNTGKLEKQ LVTLNDGLPK KPAKEIIEEL KHHLYQDFQK FFSEEKKQQY QNNFALFDRD NDKYINLSEL KELLTSVNIT FPDDELEEL YNEFCLTSPE ADGINEDAVF IIVSKKIRDN DKDEQLTQAF KLVEKAVNDE LAKTPNETKE QEGYIRVEQF K ELLMTLGN ...String:
MDNTGKLEKQ LVTLNDGLPK KPAKEIIEEL KHHLYQDFQK FFSEEKKQQY QNNFALFDRD NDKYINLSEL KELLTSVNIT FPDDELEEL YNEFCLTSPE ADGINEDAVF IIVSKKIRDN DKDEQLTQAF KLVEKAVNDE LAKTPNETKE QEGYIRVEQF K ELLMTLGN RWSEEQANEF LKDINPKSDE RINYLDVVKK LMKR

UniProtKB: Calmodulin 7-2

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Macromolecule #15: Coiled-coil domain-containing protein 153

MacromoleculeName: Coiled-coil domain-containing protein 153 / type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila (eukaryote)
Molecular weightTheoretical: 22.170178 KDa
SequenceString:
MSKKSGKGKG KNDGDELGAE KEQVLRTKVE SLIQRLGHEQ ERADRAKAAE NELRARLFDL DKDFKNEKDR LFSITSDMTR QYKQMQDEL LNQVNDLNKT VIEKDEEIKK KDQQIQDMTK DYEYKLKKKD DEIQDLKRKI EEMSAEFAKM LKDTLDKMQE R IEMVQWDS DTDPQMMKRL KDMTGLSNN

UniProtKB: Dynein regulatory complex protein 12

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

8537

Final reconstructionResolution.type: BY AUTHOR / Resolution: 7.4 Å / Resolution method: OTHER / Number images used: 211502
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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