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- EMDB-41192: Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641... -
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Open data
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Basic information
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Title | Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in mixed occluded/inward-facing conformations in lauryl maltose neopentyl glycol (LMNG) | |||||||||
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![]() | Borate transporter / Bor1 / TRANSPORT PROTEIN | |||||||||
Function / homology | ![]() borate transmembrane transport / response to boron-containing substance / cellular response to boron-containing substance levels / : / active borate transmembrane transporter activity / monoatomic ion homeostasis / solute:inorganic anion antiporter activity / vacuole / transmembrane transporter activity / transmembrane transport ...borate transmembrane transport / response to boron-containing substance / cellular response to boron-containing substance levels / : / active borate transmembrane transporter activity / monoatomic ion homeostasis / solute:inorganic anion antiporter activity / vacuole / transmembrane transporter activity / transmembrane transport / membrane => GO:0016020 / endosome / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.98 Å | |||||||||
![]() | Jiang Y / Jiang J | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of borate transporter Bor1 reveals a novel auto-inhibition mechanism for the SLC4 family Authors: Jiang Y / Jiang J | |||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
Map data | ![]() | |||
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Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 18.4 KB | Display | |
Data in CIF | ![]() | 24.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8tenMC ![]() 41185 ![]() 41186 ![]() 41187 ![]() 41188 ![]() 41190 ![]() 41191 ![]() 8tegC ![]() 8tehC ![]() 8teiC ![]() 8tejC ![]() 8telC ![]() 8temC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Arabidopsis thaliana Boron transporter 1
Entire | Name: Arabidopsis thaliana Boron transporter 1 |
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Components |
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-Supramolecule #1: Arabidopsis thaliana Boron transporter 1
Supramolecule | Name: Arabidopsis thaliana Boron transporter 1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: Boron transporter 1
Macromolecule | Name: Boron transporter 1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 80.108898 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MEETFVPFEG IKNDLKGRLM CYKQDWTGGF KAGFRILAPT TYIFFASAIP VISFGEQLER STDGVLTAVQ TLASTAICGM IHSIIGGQP LLILGVAEPT VIMYTFMFNF AKARPELGRD LFLAWSGWVC VWTALMLFVL AICGACSIIN RFTRVAGELF G LLIAMLFM ...String: MEETFVPFEG IKNDLKGRLM CYKQDWTGGF KAGFRILAPT TYIFFASAIP VISFGEQLER STDGVLTAVQ TLASTAICGM IHSIIGGQP LLILGVAEPT VIMYTFMFNF AKARPELGRD LFLAWSGWVC VWTALMLFVL AICGACSIIN RFTRVAGELF G LLIAMLFM QQAIKGLVDE FRIPERENQK LKEFLPSWRF ANGMFALVLS FGLLLTGLRS RKARSWRYGT GWLRSLIADY GV PLMVLVW TGVSYIPAGD VPKGIPRRLF SPNPWSPGAY GNWTVVKEML DVPIVYIIGA FIPASMIAVL YYFDHSVASQ LAQ QKEFNL RKPSSYHYDL LLLGFLTLMC GLLGVPPSNG VIPQSPMHTK SLATLKYQLL RNRLVATARR SIKTNASLGQ LYDN MQEAY HHMQ(TPO)PLVYQ QPQGLKELKE STIQATTFTG NLNAPVDETL FDIEKEIDDL LPVEVKEQRV SNLLQSTMVG G CVAAMPIL KMIPTSVLWG YFAFMAIESL PGNQFWERIL LLFTAPSRRF KVLEDYHATF VETVPFKTIA MFTLFQTTYL LI CFGLTWI PIAGVMFPLM IMFLIPVRQY LLPRFFKGAH LQDLDAAEYE EAPALPFNLA AETEIGSTTS YPGDLEILDE VMT ESRGRF EHTSSPKVTS SSSTPVNNRS LSQVFSPRVS GIRLGQMSPR VVGNSPKPAS CGRSPLNQSS SNHHHHHHHH HH UniProtKB: Boron transporter 1 |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 6.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 55.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: OTHER |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.98 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 132093 |
Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE / Software - Name: RELION |