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Structure paper

TitleThe Bor1 elevator transport cycle is subject to autoinhibition and activation.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 9090, Year 2024
Publish dateOct 22, 2024
AuthorsYan Jiang / Jiansen Jiang /
PubMed AbstractBoron, essential for plant growth, necessitates precise regulation due to its potential toxicity. This regulation is achieved by borate transporters (BORs), which are homologous to the SLC4 family. ...Boron, essential for plant growth, necessitates precise regulation due to its potential toxicity. This regulation is achieved by borate transporters (BORs), which are homologous to the SLC4 family. The Arabidopsis thaliana Bor1 (AtBor1) transporter from clade I undergoes slow regulation through degradation and translational suppression, but its potential for fast regulation via direct activity modulation was unclear. Here, we combine cryo-electron microscopy, mutagenesis, and functional characterization to study AtBor1, revealing high-resolution structures of the dimer in one inactive and three active states. Our findings show that AtBor1 is regulated by two distinct mechanisms: an autoinhibitory domain at the carboxyl terminus obstructs the substrate pathway via conserved salt bridges, and phosphorylation of Thr410 allows interaction with a positively charged pocket at the cytosolic face, essential for borate transport. These results elucidate the molecular basis of AtBor1's activity regulation and highlight its role in fast boron level regulation in plants.
External linksNat Commun / PubMed:39433547 / PubMed Central
MethodsEM (single particle)
Resolution2.15 - 3.02 Å
Structure data

41185

8teg

EMDB-41185, PDB-8teg:
Cryo-EM structure of Arabidopsis thaliana Bor1 in lipid nanodiscs (protomer-focused refinement)
Method: EM (single particle) / Resolution: 2.15 Å

41186

8teh

EMDB-41186, PDB-8teh:
Cryo-EM structure of Arabidopsis thaliana Bor1 in lipid nanodiscs
Method: EM (single particle) / Resolution: 2.3 Å

41187

8tei

EMDB-41187, PDB-8tei:
Cryo-EM structure of Arabidopsis thaliana Bor1 in lauryl maltose neopentyl glycol (LMNG)
Method: EM (single particle) / Resolution: 2.58 Å

41188

8tej

EMDB-41188, PDB-8tej:
Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in the occluded conformation in lauryl maltose neopentyl glycol (LMNG) (protomer-focused refinement)
Method: EM (single particle) / Resolution: 2.55 Å

41190

8tel

EMDB-41190, PDB-8tel:
Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in the occluded conformation in lauryl maltose neopentyl glycol (LMNG)
Method: EM (single particle) / Resolution: 2.77 Å

41191

8tem

EMDB-41191, PDB-8tem:
Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in the inward-facing conformation in lauryl maltose neopentyl glycol (LMNG)
Method: EM (single particle) / Resolution: 3.02 Å

41192

8ten

EMDB-41192, PDB-8ten:
Cryo-EM structure of Arabidopsis thaliana Bor1 mutant (R637E/E641R/R643E) in mixed occluded/inward-facing conformations in lauryl maltose neopentyl glycol (LMNG)
Method: EM (single particle) / Resolution: 2.98 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-POV:
(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipid*YM

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / Borate transporter / Bor1

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