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データを開く
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基本情報
| 登録情報 | ![]() | ||||||||||||||||||
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| タイトル | Structure of the 48S translation initiation complex assembled on the encephalomyocarditis virus IRES | ||||||||||||||||||
マップデータ | 40SIC-EMCV-tRNA-eIF3 | ||||||||||||||||||
試料 |
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キーワード | EMCV / IRES / Cryo-EM / 40SIC / RIBOSOME | ||||||||||||||||||
| 機能・相同性 | 機能・相同性情報viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex, eIF3m / IRES-dependent viral translational initiation / translation reinitiation / formation of cytoplasmic translation initiation complex / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / translation factor activity, RNA binding ...viral translational termination-reinitiation / eukaryotic translation initiation factor 3 complex, eIF3e / eukaryotic translation initiation factor 3 complex, eIF3m / IRES-dependent viral translational initiation / translation reinitiation / formation of cytoplasmic translation initiation complex / multi-eIF complex / eukaryotic translation initiation factor 3 complex / eukaryotic 43S preinitiation complex / translation factor activity, RNA binding / eukaryotic 48S preinitiation complex / regulation of translational initiation / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / metal-dependent deubiquitinase activity / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / laminin receptor activity / Translation initiation complex formation / Formation of a pool of free 40S subunits / 90S preribosome / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / phagocytic cup / translation regulator activity / rough endoplasmic reticulum / ribosomal small subunit export from nucleus / laminin binding / translation initiation factor binding / translation initiation factor activity / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / ribosome assembly / positive regulation of apoptotic signaling pathway / positive regulation of translation / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / PML body / spindle / fibrillar center / metallopeptidase activity / rRNA processing / positive regulation of canonical Wnt signaling pathway / rhythmic process / regulation of translation / ribosomal small subunit assembly / virus receptor activity / ribosome binding / ribosomal small subunit biogenesis / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / perikaryon / cytoplasmic translation / cell differentiation / tRNA binding / cysteine-type deubiquitinase activity / mitochondrial inner membrane / postsynaptic density / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / cell division / DNA repair / mRNA binding / apoptotic process / centrosome / synapse / dendrite / nucleolus / perinuclear region of cytoplasm / Golgi apparatus / DNA binding / RNA binding / zinc ion binding / nucleoplasm / membrane / identical protein binding / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||||||||||||||
| 生物種 | ![]() Encephalomyocarditis virus (脳心筋炎ウイルス) / Homo sapiens (ヒト) | ||||||||||||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | ||||||||||||||||||
データ登録者 | Bhattacharjee S / Abaeva IS / Brown ZP / Arhab Y / Fallah H / Jeevan JC / Hellen CUT / Frank J / Pestova TV | ||||||||||||||||||
| 資金援助 | 米国, 5件
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引用 | ジャーナル: bioRxiv / 年: 2025 タイトル: The mechanism of ribosomal recruitment during translation initiation on Type 2 IRESs. 著者: Sayan Bhattacharjee / Irina S Abaeva / Zuben P Brown / Yani Arhab / Hengameh Fallah / Christopher U T Hellen / Joachim Frank / Tatyana V Pestova / ![]() 要旨: The encephalomyocarditis virus (EMCV) IRES and other Type 2 IRESs comprise domains H-L and specifically interact with eIF4G/eIF4A through their essential JK domain. However, the JK domain is not ...The encephalomyocarditis virus (EMCV) IRES and other Type 2 IRESs comprise domains H-L and specifically interact with eIF4G/eIF4A through their essential JK domain. However, the JK domain is not sufficient for IRES function, which also requires the preceding domain I of unknown function. To identify interactions that drive ribosomal recruitment of eIF4G/eIF4A-bound Type 2 IRESs, we determined the cryo-EM structure of 48S initiation complexes formed on the EMCV IRES. It revealed that the apical domain I cloverleaf contacts ribosomal proteins uS13 and uS19 via its Id subdomain and that the essential GNRA tetraloop in subdomain Ic interacts directly with the TψC domain of initiator tRNA. Functional assays supported the exceptional role of these interactions for initiation on this IRES. The strong conservation of primary and secondary structures of the apex of domain I among Type 2 IRESs suggests that the reported interactions are a common essential feature of them all. | ||||||||||||||||||
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
| マップデータ | emd_40774.map.gz | 111.1 MB | EMDBマップデータ形式 | |
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| ヘッダ (付随情報) | emd-40774-v30.xml emd-40774.xml | 80.7 KB 80.7 KB | 表示 表示 | EMDBヘッダ |
| 画像 | emd_40774.png | 93.8 KB | ||
| Filedesc metadata | emd-40774.cif.gz | 16.5 KB | ||
| アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-40774 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-40774 | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 8supMC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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| 「今月の分子」の関連する項目 |
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マップ
| ファイル | ダウンロード / ファイル: emd_40774.map.gz / 形式: CCP4 / 大きさ: 125 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| 注釈 | 40SIC-EMCV-tRNA-eIF3 | ||||||||||||||||||||||||||||||||||||
| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 1.24 Å | ||||||||||||||||||||||||||||||||||||
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| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : EMCV IRES 40SIC
+超分子 #1: EMCV IRES 40SIC
+超分子 #2: 40SIC
+超分子 #3: EMCV IRES
+分子 #1: Eukaryotic translation initiation factor 3 subunit A
+分子 #2: Eukaryotic translation initiation factor 3 subunit C
+分子 #3: Eukaryotic translation initiation factor 3 subunit E
+分子 #4: Eukaryotic translation initiation factor 3 subunit F
+分子 #5: Eukaryotic translation initiation factor 3 subunit H
+分子 #6: Eukaryotic translation initiation factor 3 subunit K
+分子 #7: Eukaryotic translation initiation factor 3 subunit L
+分子 #8: Eukaryotic translation initiation factor 3 subunit M
+分子 #11: Eukaryotic translation initiation factor 1A, X-chromosomal
+分子 #12: uS2 (SA)
+分子 #13: eS1
+分子 #14: 40S ribosomal protein S2
+分子 #15: uS3
+分子 #16: eS4 (S4 X isoform)
+分子 #17: uS7
+分子 #18: eS6
+分子 #19: eS7
+分子 #20: eS8
+分子 #21: uS4
+分子 #22: eS10
+分子 #23: uS17
+分子 #24: eS12
+分子 #25: uS15
+分子 #26: Small ribosomal subunit protein uS11
+分子 #27: uS19
+分子 #28: uS9
+分子 #29: eS17
+分子 #30: uS13
+分子 #31: eS19
+分子 #32: uS10
+分子 #33: eS21
+分子 #34: uS8
+分子 #35: uS12
+分子 #36: eS24
+分子 #37: eS25
+分子 #38: eS26
+分子 #39: eS27
+分子 #40: eS28
+分子 #41: uS14
+分子 #42: eS30
+分子 #43: eS31
+分子 #44: Receptor for Activated C Kinase 1 (RACK1)
+分子 #46: Eukaryotic translation initiation factor 2 subunit 1
+分子 #47: eL41
+分子 #9: 18S rRNA
+分子 #10: EMCV mRNA
+分子 #45: Met-tRNAiMet
+分子 #48: ZINC ION
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
| 試料の集合状態 | cell |
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試料調製
| 緩衝液 | pH: 7.5 |
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| 凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
| 顕微鏡 | FEI TECNAI F30 |
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| 撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 58.0 e/Å2 |
| 電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: DARK FIELD / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 1.0 µm |
| 実験機器 | ![]() モデル: Tecnai F30 / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
Encephalomyocarditis virus (脳心筋炎ウイルス)
Homo sapiens (ヒト)
データ登録者
米国, 5件
引用

















Z (Sec.)
Y (Row.)
X (Col.)




















解析
FIELD EMISSION GUN
