+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-3896 | |||||||||||||||
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タイトル | Cryo-EM Structure of Saccharomyces cerevisiae Target of Rapamycin Complex 2 | |||||||||||||||
マップデータ | refined and postprocessed map | |||||||||||||||
試料 |
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キーワード | target of rapamycin / torc2 / FRB domain / Tor2-Lst8 / kinases / SIGNALING PROTEIN | |||||||||||||||
機能・相同性 | 機能・相同性情報 PIP3 activates AKT signaling / CD28 dependent PI3K/Akt signaling / regulation of snRNA pseudouridine synthesis / mitochondria-nucleus signaling pathway / Regulation of TP53 Degradation / TORC2 signaling / 1-phosphatidylinositol 4-kinase / 1-phosphatidylinositol 4-kinase activity / establishment or maintenance of actin cytoskeleton polarity / VEGFR2 mediated vascular permeability ...PIP3 activates AKT signaling / CD28 dependent PI3K/Akt signaling / regulation of snRNA pseudouridine synthesis / mitochondria-nucleus signaling pathway / Regulation of TP53 Degradation / TORC2 signaling / 1-phosphatidylinositol 4-kinase / 1-phosphatidylinositol 4-kinase activity / establishment or maintenance of actin cytoskeleton polarity / VEGFR2 mediated vascular permeability / HSF1-dependent transactivation / fungal-type cell wall organization / Amino acids regulate mTORC1 / TORC2 complex / TORC1 complex / TORC1 signaling / cellular response to nitrogen starvation / fungal-type vacuole membrane / vacuolar membrane / positive regulation of Rho protein signal transduction / negative regulation of macroautophagy / positive regulation of endocytosis / TOR signaling / cytoskeleton organization / phosphatidylinositol-4,5-bisphosphate binding / negative regulation of autophagy / protein serine/threonine kinase activator activity / response to nutrient / nuclear periphery / regulation of cell growth / regulation of actin cytoskeleton organization / ribosome biogenesis / molecular adaptor activity / regulation of cell cycle / non-specific serine/threonine protein kinase / endosome membrane / Golgi membrane / protein serine kinase activity / protein serine/threonine kinase activity / protein-containing complex binding / signal transduction / mitochondrion / ATP binding / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||||||||
生物種 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.9 Å | |||||||||||||||
データ登録者 | Karuppasamy M / Kusmider B | |||||||||||||||
資金援助 | 4件
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引用 | ジャーナル: Mol Cell / 年: 2015 タイトル: Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2. 著者: Christl Gaubitz / Taiana M Oliveira / Manoel Prouteau / Alexander Leitner / Manikandan Karuppasamy / Georgia Konstantinidou / Delphine Rispal / Sandra Eltschinger / Graham C Robinson / ...著者: Christl Gaubitz / Taiana M Oliveira / Manoel Prouteau / Alexander Leitner / Manikandan Karuppasamy / Georgia Konstantinidou / Delphine Rispal / Sandra Eltschinger / Graham C Robinson / Stéphane Thore / Ruedi Aebersold / Christiane Schaffitzel / Robbie Loewith / 要旨: Target of Rapamycin (TOR) plays central roles in the regulation of eukaryote growth as the hub of two essential multiprotein complexes: TORC1, which is rapamycin-sensitive, and the lesser ...Target of Rapamycin (TOR) plays central roles in the regulation of eukaryote growth as the hub of two essential multiprotein complexes: TORC1, which is rapamycin-sensitive, and the lesser characterized TORC2, which is not. TORC2 is a key regulator of lipid biosynthesis and Akt-mediated survival signaling. In spite of its importance, its structure and the molecular basis of its rapamycin insensitivity are unknown. Using crosslinking-mass spectrometry and electron microscopy, we determined the architecture of TORC2. TORC2 displays a rhomboid shape with pseudo-2-fold symmetry and a prominent central cavity. Our data indicate that the C-terminal part of Avo3, a subunit unique to TORC2, is close to the FKBP12-rapamycin-binding domain of Tor2. Removal of this sequence generated a FKBP12-rapamycin-sensitive TORC2 variant, which provides a powerful tool for deciphering TORC2 function in vivo. Using this variant, we demonstrate a role for TORC2 in G2/M cell-cycle progression. | |||||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_3896.map.gz | 113.2 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-3896-v30.xml emd-3896.xml | 28.4 KB 28.4 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_3896.png | 43.5 KB | ||
Filedesc metadata | emd-3896.cif.gz | 9.4 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-3896 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-3896 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_3896_validation.pdf.gz | 237.4 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_3896_full_validation.pdf.gz | 236.5 KB | 表示 | |
XML形式データ | emd_3896_validation.xml.gz | 6.4 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-3896 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-3896 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_3896.map.gz / 形式: CCP4 / 大きさ: 125 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | refined and postprocessed map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.38 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : Target of rapamycin protein complex 2
全体 | 名称: Target of rapamycin protein complex 2 |
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要素 |
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-超分子 #1: Target of rapamycin protein complex 2
超分子 | 名称: Target of rapamycin protein complex 2 / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all |
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由来(天然) | 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) |
分子量 | 理論値: 1.4 MDa |
-分子 #1: Serine/threonine-protein kinase TOR2
分子 | 名称: Serine/threonine-protein kinase TOR2 / タイプ: protein_or_peptide / ID: 1 / コピー数: 2 / 光学異性体: LEVO / EC番号: 1-phosphatidylinositol 4-kinase |
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由来(天然) | 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) |
分子量 | 理論値: 281.915438 KDa |
組換発現 | 生物種: Saccharomyces cerevisiae (パン酵母) |
配列 | 文字列: MNKYINKYTT PPNLLSLRQR AEGKHRTRKK LTHKSHSHDD EMSTTSNTDS NHNGPNDSGR VITGSAGHIG KISFVDSELD TTFSTLNLI FDKLKSDVPQ ERASGANELS TTLTSLAREV SAEQFQRFSN SLNNKIFELI HGFTSSEKIG GILAVDTLIS F YLSTEELP ...文字列: MNKYINKYTT PPNLLSLRQR AEGKHRTRKK LTHKSHSHDD EMSTTSNTDS NHNGPNDSGR VITGSAGHIG KISFVDSELD TTFSTLNLI FDKLKSDVPQ ERASGANELS TTLTSLAREV SAEQFQRFSN SLNNKIFELI HGFTSSEKIG GILAVDTLIS F YLSTEELP NQTSRLANYL RVLIPSSDIE VMRLAANTLG RLTVPGGTLT SDFVEFEVRT CIDWLTLTAD NNSSSSKLEY RR HAALLII KALADNSPYL LYPYVNSILD NIWVPLRDAK LIIRLDAAVA LGKCLTIIQD RDPALGKQWF QRLFQGCTHG LSL NTNDSV HATLLVFREL LSLKAPYLRD KYDDIYKSTM KYKEYKFDVI RREVYAILPL LAAFDPAIFT KKYLDRIMVH YLRY LKNID MNAANNSDKP FILVSIGDIA FEVGSSISPY MTLILDNIRE GLRTKFKVRK QFEKDLFYCI GKLACALGPA FAKHL NKDL LNLMLNCPMS DHMQETLMIL NEKIPSLEST VNSRILNLLS ISLSGEKFIQ SNQYDFNNQF SIEKARKSRN QSFMKK TGE SNDDITDAQI LIQCFKMLQL IHHQYSLTEF VRLITISYIE HEDSSVRKLA ALTSCDLFIK DDICKQTSVH ALHSVSE VL SKLLMIAITD PVAEIRLEIL QHLGSNFDPQ LAQPDNLRLL FMALNDEIFG IQLEAIKIIG RLSSVNPAYV VPSLRKTL L ELLTQLKFSN MPKKKEESAT LLCTLINSSD EVAKPYIDPI LDVILPKCQD ASSAVASTAL KVLGELSVVG GKEMTRYLK ELMPLIINTF QDQSNSFKRD AALTTLGQLA ASSGYVVGPL LDYPELLGIL INILKTENNP HIRRGTVRLI GILGALDPYK HREIEVTSN SKSSVEQNAP SIDIALLMQG VSPSNDEYYP TVVIHNLMKI LNDPSLSIHH TAAIQAIMHI FQNLGLRCVS F LDQIIPGI ILVMRSCPPS QLDFYFQQLG SLISIVKQHI RPHVEKIYGV IREFFPIIKL QITIISVIES ISKALEGEFK RF VPETLTF FLDILENDQS NKRIVPIRIL KSLVTFGPNL EDYSHLIMPI VVRMTEYSAG SLKKISIITL GRLAKNINLS EMS SRIVQA LVRILNNGDR ELTKATMNTL SLLLLQLGTD FVVFVPVINK ALLRNRIQHS VYDQLVNKLL NNECLPTNII FDKE NEVPE RKNYEDEMQV TKLPVNQNIL KNAWYCSQQK TKEDWQEWIR RLSIQLLKES PSACLRSCSS LVSVYYPLAR ELFNA SFSS CWVELQTSYQ EDLIQALCKA LSSSENPPEI YQMLLNLVEF MEHDDKPLPI PIHTLGKYAQ KCHAFAKALH YKEVEF LEE PKNSTIEALI SINNQLHQTD SAIGILKHAQ QHNELQLKET WYEKLQRWED ALAAYNEKEA AGEDSVEVMM GKLRSLY AL GEWEELSKLA SEKWGTAKPE VKKAMAPLAA GAAWGLEQWD EIAQYTSVMK SQSPDKEFYD AILCLHRNNF KKAEVHIF N ARDLLVTELS ALVNESYNRA YNVVVRAQII AELEEIIKYK KLPQNSDKRL TMRETWNTRL LGCQKNIDVW QRILRVRSL VIKPKEDAQV RIKFANLCRK SGRMALAKKV LNTLLEETDD PDHPNTAKAS PPVVYAQLKY LWATGLQDEA LKQLINFTSR MAHDLGLDP NNMIAQSVPQ QSKRVPRHVE DYTKLLARCF LKQGEWRVCL QPKWRLSNPD SILGSYLLAT HFDNTWYKAW H NWALANFE VISMLTSVSK KKQEGSDASS VTDINEFDNG MIGVNTFDAK EVHYSSNLIH RHVIPAIKGF FHSISLSESS SL QDALRLL TLWFTFGGIP EATQAMHEGF NLIQIGTWLE VLPQLISRIH QPNQIVSRSL LSLLSDLGKA HPQALVYPLM VAI KSESLS RQKAALSIIE KMRIHSPVLV DQAELVSHEL IRMAVLWHEQ WYEGLDDASR QFFGEHNTEK MFAALEPLYE MLKR GPETL REISFQNSFG RDLNDAYEWL MNYKKSKDVS NLNQAWDIYY NVFRKIGKQL PQLQTLELQH VSPKLLSAHD LELAV PGTR ASGGKPIVKI SKFEPVFSVI SSKQRPRKFC IKGSDGKDYK YVLKGHEDIR QDSLVMQLFG LVNTLLQNDA ECFRRH LDI QQYPAIPLSP KSGLLGWVPN SDTFHVLIRE HREAKKIPLN IEHWVMLQMA PDYDNLTLLQ KVEVFTYALN NTEGQDL YK VLWLKSRSSE TWLERRTTYT RSLAVMSMTG YILGLGDRHP SNLMLDRITG KVIHIDFGDC FEAAILREKF PEKVPFRL T RMLTYAMEVS GIEGSFRITC ENVMKVLRDN KGSLMAILEA FAFDPLINWG FDLPTKKIEE ETGIQLPVMN ANELLSNGA ITEEEVQRVE NEHKNAIRNA RAMLVLKRIT DKLTGNDIRR FNDLDVPEQV DKLIQQATSV ENLCQHYIGW CPFW UniProtKB: Serine/threonine-protein kinase TOR2 |
-分子 #2: Target of rapamycin complex subunit LST8
分子 | 名称: Target of rapamycin complex subunit LST8 / タイプ: protein_or_peptide / ID: 2 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) |
分子量 | 理論値: 34.077879 KDa |
組換発現 | 生物種: Saccharomyces cerevisiae (パン酵母) |
配列 | 文字列: MSVILVSAGY DHTIRFWEAL TGVCSRTIQH SDSQVNRLEI TNDKKLLATA GHQNVRLYDI RTTNPNPVAS FEGHRGNVTS VSFQQDNRW MVTSSEDGTI KVWDVRSPSI PRNYKHNAPV NEVVIHPNQG ELISCDRDGN IRIWDLGENQ CTHQLTPEDD T SLQSLSMA ...文字列: MSVILVSAGY DHTIRFWEAL TGVCSRTIQH SDSQVNRLEI TNDKKLLATA GHQNVRLYDI RTTNPNPVAS FEGHRGNVTS VSFQQDNRW MVTSSEDGTI KVWDVRSPSI PRNYKHNAPV NEVVIHPNQG ELISCDRDGN IRIWDLGENQ CTHQLTPEDD T SLQSLSMA SDGSMLAAAN TKGNCYVWEM PNHTDASHLK PVTKFRAHST YITRILLSSD VKHLATCSAD HTARVWSIDD DF KLETTLD GHQRWVWDCA FSADSAYLVT ASSDHYVRLW DLSTREIVRQ YGGHHKGAVC VALNDV UniProtKB: Target of rapamycin complex subunit LST8 |
-分子 #3: Target of rapamycin complex 2 subunit TSC11
分子 | 名称: Target of rapamycin complex 2 subunit TSC11 / タイプ: protein_or_peptide / ID: 3 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) |
分子量 | 理論値: 25.804662 KDa |
組換発現 | 生物種: Saccharomyces cerevisiae (パン酵母) |
配列 | 文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) |
-分子 #4: Target of rapamycin complex 2 subunit AVO2
分子 | 名称: Target of rapamycin complex 2 subunit AVO2 / タイプ: protein_or_peptide / ID: 4 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) |
分子量 | 理論値: 47.206457 KDa |
組換発現 | 生物種: Saccharomyces cerevisiae (パン酵母) |
配列 | 文字列: MLKEPSVRLR EAIIEGNLLI VKRLLRRNPD LLTNIDSENG WSSLHYASYH GRYLICVYLI QLGHDKHELI KTFKGNTCVH LALMKGHEQ TLHLLLQQFP RFINHRGENG RAPIHIACMN DYYQCLSLLI GVGADLWVMD TNGDTPLHVC LEYGSISCMK M LLNEGEVS ...文字列: MLKEPSVRLR EAIIEGNLLI VKRLLRRNPD LLTNIDSENG WSSLHYASYH GRYLICVYLI QLGHDKHELI KTFKGNTCVH LALMKGHEQ TLHLLLQQFP RFINHRGENG RAPIHIACMN DYYQCLSLLI GVGADLWVMD TNGDTPLHVC LEYGSISCMK M LLNEGEVS LDDNVRDKGN WKPIDVAQTF EVGNIYSKVL KEVKKKGPPL GAGKKPSSFR TPILNAKATF EDGPSPVLSM NS PYSLYSN NSPLPVLPRR ISTHTTSGNG GNRRSSITNP VFNPRKPTLS TDSFSSSSNS SSRLRVNSIN VKTPVGVSPK KEL VSESVR HSATPTSPHN NIALINRYLL PNKSNDNVRG DSQTATINDD GGGGNGGDAT IGMGLRKDPD DENENKYKIK VNNG EPRRR VSLLNIPISK LRNSNNTRAE D UniProtKB: Target of rapamycin complex 2 subunit AVO2 |
-分子 #5: Target of rapamycin complex 2 subunit AVO1
分子 | 名称: Target of rapamycin complex 2 subunit AVO1 / タイプ: protein_or_peptide / ID: 5 / コピー数: 2 / 光学異性体: LEVO |
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由来(天然) | 生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (パン酵母) |
分子量 | 理論値: 131.565453 KDa |
組換発現 | 生物種: Saccharomyces cerevisiae (パン酵母) |
配列 | 文字列: MDTVTVLNEL RAQFLRVCPE KDQMKRIIKP YIPVDEFNTE QCLDSSIREL YMNSDGVSLL PELESPPVSK DFMENYASLG KMRIMRENE GQKGKANQNL IGAEKTERDE EETRNLQDKS AKNTLIVEEN GTLRYNPLNS SASNSLLNDD DHTSGKHHKT S SKEDSYLN ...文字列: MDTVTVLNEL RAQFLRVCPE KDQMKRIIKP YIPVDEFNTE QCLDSSIREL YMNSDGVSLL PELESPPVSK DFMENYASLG KMRIMRENE GQKGKANQNL IGAEKTERDE EETRNLQDKS AKNTLIVEEN GTLRYNPLNS SASNSLLNDD DHTSGKHHKT S SKEDSYLN SSMEMQKKSS KRSSLPFVRI FKSRRDHSNT SGNKNVMNTT NTRAKSSTLH PPGARHNKKG SKFDMNFDFD EN LEEEDDD DDDDEEGDDI HSQFFQLDDD FDAKGSGASP AHKGINGMSN NKNNTYTNNR NSISILDDRE SSNGNIGSAS RLK SHFPTS QKGKIFLTDN KNDGQKSDSL NANKGIHGDG SSASGNGSVS RDGLTETESN NISDMESYIN EKDLDDLNFD TVTS NINKT VSDLGGHEST NDGTAVMNRD SKDSRSNSNE FNAQNRDRIT PGSSYGKSLL GSEYSEERYS NNDSSTMESG EMSLD SDMQ TNTIPSHSIP MSMQKYGIYH GDDDSTLNNV FDKAVLTMNS SRHPKERRDT VISGKEPTSL TSSNRKFSVS SNLTST RSP LLRGHGRTSS TASSEHMKAP KVSDSVLHRA RKSTLTLKQD HSQPSVPSSV HKSSKEGNIL IEKTTDYLVS KPKASQL SN MFNKKKKRTN TNSVDVLEYF SFVCGDKVPN YESMGLEIYI QASKKYKRNS FTTKVRKSST IFEVIGFALF LYSTEKKP D NFEEDGLTVE DISNPNNFSL KIVDEDGEPF EDNFGKLDRK STIQSISDSE VVLCKVDDAE KSQNEIETPL PFETGGGLM DASTLDANSS HDTTDGTINQ LSFYKPIIGN EDDIDKTNGS KIIDVTVYLY PNVNPKFNYT TISVLVTSHI NDILVKYCKM KNMDPNEYA LKVLGKNYIL DLNDTVLRLD GINKVELISK KDARELHLEK MKPDLKKPVL PTIQSNDLTP LTLEPLNSYL K ADAGGAVA AIPENTKVTS KAKKISTKYK LGLAKQHSSS SVASGSVSTA GGLANGNGFF KNKNSSKSSL HGTLQFHNIN RS QSTMEHT PDTPNGVGDN FQDLFTGAYH KYKVWRRQQM SFINKHERTL AIDGDYIYIV PPEGRIHWHD NVKTKSLHIS QVV LVKKSK RVPEHFKIFV RREGQDDIKR YYFEAVSGQE CTEIVTRLQN LLSAYRMNHK UniProtKB: Target of rapamycin complex 2 subunit AVO1 |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 |
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グリッド | モデル: Quantifoil R2/2 / 材質: COPPER / メッシュ: 300 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 3 / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 60 sec. / 前処理 - 雰囲気: AIR / 前処理 - 気圧: 0.01 kPa |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 4 K / 装置: FEI VITROBOT MARK IV / 詳細: 2 - 3 sec blotting. |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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温度 | 最低: 70.0 K / 最高: 70.0 K |
撮影 | #0 - Image recording ID: 1 #0 - フィルム・検出器のモデル: FEI FALCON II (4k x 4k) #0 - 検出モード: INTEGRATING / #0 - 撮影したグリッド数: 4 / #0 - 実像数: 4189 / #0 - 平均露光時間: 2.3 sec. / #0 - 平均電子線量: 50.0 e/Å2 / #1 - Image recording ID: 2 #1 - フィルム・検出器のモデル: GATAN K2 QUANTUM (4k x 4k) #1 - 検出モード: SUPER-RESOLUTION / #1 - デジタル化 - サイズ - 横: 3710 pixel / #1 - デジタル化 - サイズ - 縦: 3838 pixel / #1 - デジタル化 - 画像ごとのフレーム数: 1-40 / #1 - 撮影したグリッド数: 1 / #1 - 実像数: 2847 / #1 - 平均露光時間: 20.0 sec. / #1 - 平均電子線量: 47.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | C2レンズ絞り径: 50.0 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 3.5 µm / 最小 デフォーカス(公称値): 1.5 µm / 倍率(公称値): 105000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |