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- EMDB-3766: Cryo-EM structure of a pre-catalytic human spliceosome primed for... -

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Basic information

Entry
Database: EMDB / ID: EMD-3766
TitleCryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex)
Map datasharpened, refined map
Sample
  • Complex: Human B Complex Spliceosome, sharpened map, masked refinement
    • Protein or peptide: x 38 types
    • RNA: x 5 types
Function / homology
Function and homology information


microfibril / Lsm2-8 complex / U6 snRNA 3'-end binding / spliceosomal snRNP complex / ribonucleoprotein complex localization / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / U4atac snRNA binding ...microfibril / Lsm2-8 complex / U6 snRNA 3'-end binding / spliceosomal snRNP complex / ribonucleoprotein complex localization / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / U4atac snRNA binding / mRNA decay by 5' to 3' exoribonuclease / Lsm1-7-Pat1 complex / U6 snRNP / box C/D sno(s)RNA binding / U11/U12 snRNP / PH domain binding / dense fibrillar component / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / B-WICH complex / histone pre-mRNA 3'end processing complex / cis assembly of pre-catalytic spliceosome / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / box C/D methylation guide snoRNP complex / protein methylation / splicing factor binding / U4/U6 snRNP / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / U12-type spliceosomal complex / methylosome / 7-methylguanosine cap hypermethylation / protein localization to kinetochore / U1 snRNP binding / pICln-Sm protein complex / P-body assembly / sno(s)RNA-containing ribonucleoprotein complex / snRNP binding / U2-type catalytic step 1 spliceosome / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / U4 snRNA binding / SMN-Sm protein complex / telomerase RNA binding / spliceosomal tri-snRNP complex / telomerase holoenzyme complex / P granule / U2-type spliceosomal complex / U2-type precatalytic spliceosome / proline-rich region binding / mRNA cis splicing, via spliceosome / U3 snoRNA binding / commitment complex / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / box C/D snoRNP assembly / U4 snRNP / U2 snRNP / SAGA complex / rRNA modification in the nucleus and cytosol / RNA Polymerase II Transcription Termination / positive regulation of transcription by RNA polymerase III / U1 snRNP / U2-type prespliceosome / tRNA processing / regulation of alternative mRNA splicing, via spliceosome / precatalytic spliceosome / K63-linked polyubiquitin modification-dependent protein binding / cyclosporin A binding / positive regulation of transcription by RNA polymerase I / mitotic spindle assembly checkpoint signaling / mRNA Splicing - Minor Pathway / spliceosomal complex assembly / mRNA catabolic process / regulation of RNA splicing / nuclear-transcribed mRNA catabolic process / protein peptidyl-prolyl isomerization / MLL1 complex / spliceosomal tri-snRNP complex assembly / single fertilization / U5 snRNA binding / U5 snRNP / Major pathway of rRNA processing in the nucleolus and cytosol / positive regulation of viral genome replication / U2 snRNA binding / U6 snRNA binding / ribonucleoprotein complex binding / spliceosomal snRNP assembly / regulation of DNA repair / Cajal body / RNA processing / pre-mRNA intronic binding / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / mRNA Splicing - Major Pathway / RNA splicing
Similarity search - Function
Protein RED, C-terminal / RED-like, N-terminal / Protein Red-like / RED-like protein C-terminal region / RED-like protein N-terminal region / WD40 repeat-containing protein SMU1 / WW domain-binding protein 4 / Pre-mRNA-splicing factor 38, C-terminal / Pre-mRNA-splicing factor 38-associated hydrophilic C-term / Micro-fibrillar-associated protein 1, C-terminal ...Protein RED, C-terminal / RED-like, N-terminal / Protein Red-like / RED-like protein C-terminal region / RED-like protein N-terminal region / WD40 repeat-containing protein SMU1 / WW domain-binding protein 4 / Pre-mRNA-splicing factor 38, C-terminal / Pre-mRNA-splicing factor 38-associated hydrophilic C-term / Micro-fibrillar-associated protein 1, C-terminal / Microfibrillar-associated protein 1 / Microfibril-associated/Pre-mRNA processing / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / : / LisH-like dimerisation domain / Pre-mRNA-splicing factor 38 / PRP38 family / U1-C, C2H2-type zinc finger / U1 zinc finger / PRP4-like superfamily / pre-mRNA processing factor 4 (PRP4) like / C-terminal to LisH motif. / CTLH, C-terminal LisH motif / C-terminal to LisH (CTLH) motif profile. / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / Sm-like protein Lsm8 / PWI domain superfamily / PWI domain / PWI domain profile. / U6 snRNA-associated Sm-like protein Lsm1/8 / Pre-mRNA processing factor 4 (PRP4)-like / U6 snRNA-associated Sm-like protein LSm2 / Sm-like protein Lsm4 / Splicing Factor Motif, present in Prp18 and Pr04 / Sm-like protein Lsm7 / Sm-like protein LSm5 / Sm-like protein Lsm3 / U6 snRNA-associated Sm-like protein Lsm3 / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp3 / pre-mRNA processing factor 3 domain / Prp31 C-terminal / U4/U6 small nuclear ribonucleoprotein Prp31 / Prp31 C terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / PWI domain / PWI, domain in splicing factors / Dim1 family / Mitosis protein DIM1 / Mitosis protein DIM1 / PRP1 splicing factor, N-terminal / PRP1 splicing factor, N-terminal / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / Splicing factor 3B, subunit 5 / Splicing factor 3B subunit 1 / Splicing factor 3B subunit 1 / : / Lissencephaly type-1-like homology motif / PHF5-like / PHF5-like protein / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / Splicing factor 3B subunit 1-like / Matrin/U1-C, C2H2-type zinc finger / Zinc finger matrin-type profile. / Small ribonucleoprotein associated, SmB/SmN / Zinc-finger double-stranded RNA-binding / Zinc finger, double-stranded RNA binding / Small nuclear ribonucleoprotein D1 / H/ACA ribonucleoprotein complex, subunit Nhp2-like / NOSIC / NOSIC (NUC001) domain / Nop domain / Nop domain superfamily / Nop, C-terminal domain / snoRNA binding domain, fibrillarin / Nop domain profile. / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / PPP2R1A-like HEAT repeat / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / Tetratricopeptide repeat / Sec63 Brl domain / : / Leucine-rich repeat / Pre-mRNA-splicing factor Syf1-like / LIS1 homology (LisH) motif profile. / LIS1 homology motif / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus
Similarity search - Domain/homology
U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / Peptidyl-prolyl cis-trans isomerase H / Splicing factor 3B subunit 1 / WW domain-binding protein 4 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-processing factor 6 / U6 snRNA-associated Sm-like protein LSm8 / U2 small nuclear ribonucleoprotein B'' ...U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / Peptidyl-prolyl cis-trans isomerase H / Splicing factor 3B subunit 1 / WW domain-binding protein 4 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-processing factor 6 / U6 snRNA-associated Sm-like protein LSm8 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Microfibrillar-associated protein 1 / NHP2-like protein 1 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / U6 snRNA-associated Sm-like protein LSm3 / U6 snRNA-associated Sm-like protein LSm6 / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Thioredoxin-like protein 4A / Protein Red / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing factor 3B subunit 3 / WD40 repeat-containing protein SMU1 / Pre-mRNA-processing-splicing factor 8 / PHD finger-like domain-containing protein 5A / Pre-mRNA-splicing factor 38A / U4/U6 small nuclear ribonucleoprotein Prp31 / U5 small nuclear ribonucleoprotein 40 kDa protein / Zinc finger matrin-type protein 2 / Splicing factor 3B subunit 5 / U6 snRNA-associated Sm-like protein LSm7 / U6 snRNA-associated Sm-like protein LSm2 / U6 snRNA-associated Sm-like protein LSm5 / U6 snRNA-associated Sm-like protein LSm4
Similarity search - Component
Biological speciesHomo sapiens (human) / Human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.5 Å
AuthorsBertram K
Funding support Germany, 3 items
OrganizationGrant numberCountry
Max Planck Society Germany
German Research FoundationLU294/15-1 Germany
German Research FoundationSFB 860 Germany
CitationJournal: Cell / Year: 2017
Title: Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Authors: Karl Bertram / Dmitry E Agafonov / Olexandr Dybkov / David Haselbach / Majety N Leelaram / Cindy L Will / Henning Urlaub / Berthold Kastner / Reinhard Lührmann / Holger Stark /
Abstract: Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B ...Little is known about the spliceosome's structure before its extensive remodeling into a catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human spliceosomal B complex. The U2 snRNP-containing head domain is connected to the B complex main body via three main bridges. U4/U6.U5 tri-snRNP proteins, which are located in the main body, undergo significant rearrangements during tri-snRNP integration into the B complex. These include formation of a partially closed Prp8 conformation that creates, together with Dim1, a 5' splice site (ss) binding pocket, displacement of Sad1, and rearrangement of Brr2 such that it contacts its U4/U6 substrate and is poised for the subsequent spliceosome activation step. The molecular organization of several B-specific proteins suggests that they are involved in negatively regulating Brr2, positioning the U6/5'ss helix, and stabilizing the B complex structure. Our results indicate significant differences between the early activation phase of human and yeast spliceosomes.
History
DepositionJun 20, 2017-
Header (metadata) releaseAug 16, 2017-
Map releaseAug 16, 2017-
UpdateNov 25, 2020-
Current statusNov 25, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5o9z
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3766.png

Downloads & links

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Map

FileDownload / File: emd_3766.map.gz / Format: CCP4 / Size: 307.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationsharpened, refined map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.16 Å/pix.
x 432 pix.
= 501.12 Å		1.16 Å/pix.
x 432 pix.
= 501.12 Å		1.16 Å/pix.
x 432 pix.
= 501.12 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.16 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.04 / Movie #1: 0.04
Minimum - Maximum-0.040911388 - 0.14417519
Average (Standard dev.)0.00057586766 (±0.0069912123)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions432432432
Spacing432432432
CellA=B=C: 501.12 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.161.161.16
M x/y/z432432432
origin x/y/z0.0000.0000.000
length x/y/z501.120501.120501.120
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS432432432
D min/max/mean-0.0410.1440.001

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Supplemental data

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Additional map: None

Fileemd_3766_additional.map
AnnotationNone
Projections & Slices
AxesZYX

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Half map: None

Fileemd_3766_half_map_1.map
AnnotationNone
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: None

Fileemd_3766_half_map_2.map
AnnotationNone
Projections & Slices
AxesZYX

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Sample components

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Entire : Human B Complex Spliceosome, sharpened map, masked refinement

EntireName: Human B Complex Spliceosome, sharpened map, masked refinement
Components
  • Complex: Human B Complex Spliceosome, sharpened map, masked refinement
    • Protein or peptide: Pre-mRNA-processing-splicing factor 8
    • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
    • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
    • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp3
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp4
    • Protein or peptide: Pre-mRNA-processing factor 6
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein Prp31
    • Protein or peptide: Pre-mRNA-splicing factor 38A
    • Protein or peptide: Thioredoxin-like protein 4A
    • Protein or peptide: Microfibrillar-associated protein 1
    • Protein or peptide: WD40 repeat-containing protein SMU1
    • Protein or peptide: Peptidyl-prolyl cis-trans isomerase H
    • Protein or peptide: Zinc finger matrin-type protein 2
    • Protein or peptide: NHP2-like protein 1
    • Protein or peptide: U4/U6.U5 tri-snRNP-associated protein 1
    • Protein or peptide: WW domain-binding protein 4
    • Protein or peptide: Protein Red
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
    • Protein or peptide: Small nuclear ribonucleoprotein F
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm2
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm3
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm4
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm5
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm6
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm7
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm8
    • Protein or peptide: Splicing factor 3B subunit 1
    • Protein or peptide: Splicing factor 3B subunit 3 (SF3B3)
    • Protein or peptide: Splicing factor 3B subunit 5
    • Protein or peptide: PHD finger-like domain-containing protein 5A
    • Protein or peptide: U2 small nuclear ribonucleoprotein A'
    • Protein or peptide: U2 small nuclear ribonucleoprotein B''
    • RNA: MINX pre-mRNA
    • RNA: Human gene for small nuclear RNA U2 (snRNA U2)
    • RNA: Homo sapiens U4A snRNA
    • RNA: Homo sapiens U5 A small nuclear RNA
    • RNA: Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA

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Supramolecule #1: Human B Complex Spliceosome, sharpened map, masked refinement

SupramoleculeName: Human B Complex Spliceosome, sharpened map, masked refinement
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Pre-mRNA-processing-splicing factor 8

MacromoleculeName: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 273.97425 KDa
SequenceString: MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP ...String:
MAGVFPYRGP GNPVPGPLAP LPDYMSEEKL QEKARKWQQL QAKRYAEKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYL GALKYMPHAV LKLLENMPMP WEQIRDVPVL YHITGAISFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR H FKRMRFPP FDDEEPPLDY ADNILDVEPL EAIQLELDPE EDAPVLDWFY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YR LANQLLT DLVDDNYFYL FDLKAFFTSK ALNMAIPGGP KFEPLVRDIN LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLY NNLPHH VHLTWYHTPN VVFIKTEDPD LPAFYFDPLI NPISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTAN GIALL WAPRPFNLRS GRTRRALDIP LVKNWYREHC PAGQPVKVRV SYQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATK FFQS TKLDWVEVGL QVCRQGYNML NLLIHRKNLN YLHLDYNFNL KPVKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDS HVQ YRLGNVDAFQ LADGLQYIFA HVGQLTGMYR YKYKLMRQIR MCKDLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFF MR GITPLLERWL GNLLARQFEG RHSKGVAKTV TKQRVESHFD LELRAAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCW K ANIPWKVPGL PTPIENMILR YVKAKADWWT NTAHYNRERI RRGATVDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPY ITAEEAVAVY TTTVHWLESR RFSPIPFPPL SYKHDTKLLI LALERLKEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQR AFKEVGIEFM DLYSHLVPVY DVEPLEKITD AYLDQYLWYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI N NLQDVWET SEGECNVMLE SRFEKMYEKI DLTLLNRLLR LIVDHNIADY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FI VQYYGLV MDLLVLGLHR ASEMAGPPQM PNDFLSFQDI ATEAAHPIRL FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDP NNENIV GYNNKKCWPR DARMRLMKHD VNLGRAVFWD IKNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKC RTSYE EFTHKDGVWN LQNEVTKERT AQCFLRVDDE SMQRFHNRVR QILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVN TQEL LDLLVKCENK IQTRIKIGLN SKMPSRFPPV VFYTPKELGG LGMLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHE EDQ LIPNLYRYIQ PWESEFIDSQ RVWAEYALKR QEAIAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRT DF KQYQVLKQNP FWWTHQRHDG KLWNLNNYRT DMIQALGGVE GILEHTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTN A QRSGLNQIPN RRFTLWWSPT INRANVYVGF QVQLDLTGIF MHGKIPTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQE LDALEIETVQ KETIHPRKSY KMNSSCADIL LFASYKWNVS RPSLLADSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTT DNMSIYPSPT GVLIAIDLAY NLHSAYGNWF PGSKPLIQQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT E PYLSSQNY GELFSNQIIW FVDDTNVYRV TIHKTFEGNL TTKPINGAIF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WK TAEEVAA LIRSLPVEEQ PKQIIVTRKG MLDPLEVHLL DFPNIVIKGS ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLY DDWLKT ISSYTAFSRL ILILRALHVN NDRAKVILKP DKTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVA SLTQS EIRDIILGME ISAPSQQRQQ IAEIEKQTKE QSQLTATQTR TVNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISA ANLH LRTNHIYVSS DDIKETGYTY ILPKNVLKKF ICISDLRAQI AGYLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLP GQL PQHEYLKEME PLGWIHTQPN ESPQLSPQDV TTHAKIMADN PSWDGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQ NT DKGNNPKGYL PSHYERVQML LSDRFLGFFM VPAQSSWNYN FMGVRHDPNM KYELQLANPK EFYHEVHRPS HFLNFALL Q EGEVYSADRE DLYA

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Macromolecule #2: 116 kDa U5 small nuclear ribonucleoprotein component

MacromoleculeName: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 109.560625 KDa
SequenceString: MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT ...String:
MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKFFDDPMLL E LAKQDVVL NYPM

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Macromolecule #3: U5 small nuclear ribonucleoprotein 200 kDa helicase

MacromoleculeName: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 244.823422 KDa
SequenceString: MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR ...String:
MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR YHVLVNLGKK ITDYGGDKEI QNMDDNIDET YGVNVQFESD EEEGDEDVYG EVREEASDDD MEGDEAVVRC TL SANLVAS GELMSSKKKD LHPRDIDAFW LQRQLSRFYD DAIVSQKKAD EVLEILKTAS DDRECENQLV LLLGFNTFDF IKV LRQHRM MILYCTLLAS AQSEAEKERI MGKMEADPEL SKFLYQLHET EKEDLIREER SRRERVRQSR MDTDLETMDL DQGG EALAP RQVLDLEDLV FTQGSHFMAN KRCQLPDGSF RRQRKGYEEV HVPALKPKPF GSEEQLLPVE KLPKYAQAGF EGFKT LNRI QSKLYRAALE TDENLLLCAP TGAGKTNVAL MCMLREIGKH INMDGTINVD DFKIIYIAPM RSLVQEMVGS FGKRLA TYG ITVAELTGDH QLCKEEISAT QIIVCTPEKW DIITRKGGER TYTQLVRLII LDEIHLLHDD RGPVLEALVA RAIRNIE MT QEDVRLIGLS ATLPNYEDVA TFLRVDPAKG LFYFDNSFRP VPLEQTYVGI TEKKAIKRFQ IMNEIVYEKI MEHAGKNQ V LVFVHSRKET GKTARAIRDM CLEKDTLGLF LREGSASTEV LRTEAEQCKN LELKDLLPYG FAIHHAGMTR VDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPI ESQMVSKLPD MLNAEIVLGN VQNAKDAVNW LGYAYLYIRM LRSPTLYGIS HDDLKGDPLL DQRRLDLVHT A ALMLDKNN LVKYDKKTGN FQVTELGRIA SHYYITNDTV QTYNQLLKPT LSEIELFRVF SLSSEFKNIT VREEEKLELQ KL LERVPIP VKESIEEPSA KINVLLQAFI SQLKLEGFAL MADMVYVTQS AGRLMRAIFE IVLNRGWAQL TDKTLNLCKM IDK RMWQSM CPLRQFRKLP EEVVKKIEKK NFPFERLYDL NHNEIGELIR MPKMGKTIHK YVHLFPKLEL SVHLQPITRS TLKV ELTIT PDFQWDEKVH GSSEAFWILV EDVDSEVILH HEYFLLKAKY AQDEHLITFF VPVFEPLPPQ YFIRVVSDRW LSCET QLPV SFRHLILPEK YPPPTELLDL QPLPVSALRN SAFESLYQDK FPFFNPIQTQ VFNTVYNSDD NVFVGAPTGS GKTICA EFA ILRMLLQSSE GRCVYITPME ALAEQVYMDW YEKFQDRLNK KVVLLTGETS TDLKLLGKGN IIISTPEKWD ILSRRWK QR KNVQNINLFV VDEVHLIGGE NGPVLEVICS RMRYISSQIE RPIRIVALSS SLSNAKDVAH WLGCSATSTF NFHPNVRP V PLELHIQGFN ISHTQTRLLS MAKPVYHAIT KHSPKKPVIV FVPSRKQTRL TAIDILTTCA ADIQRQRFLH CTEKDLIPY LEKLSDSTLK ETLLNGVGYL HEGLSPMERR LVEQLFSSGA IQVVVASRSL CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP IYDVLQMVG HANRPLQDDE GRCVIMCQGS KKDFFKKFLY EPLPVESHLD HCMHDHFNAE IVTKTIENKQ DAVDYLTWTF L YRRMTQNP NYYNLQGISH RHLSDHLSEL VEQTLSDLEQ SKCISIEDEM DVAPLNLGMI AAYYYINYTT IELFSMSLNA KT KVRGLIE IISNAAEYEN IPIRHHEDNL LRQLAQKVPH KLNNPKFNDP HVKTNLLLQA HLSRMQLSAE LQSDTEEILS KAI RLIQAC VDVLSSNGWL SPALAAMELA QMVTQAMWSK DSYLKQLPHF TSEHIKRCTD KGVESVFDIM EMEDEERNAL LQLT DSQIA DVARFCNRYP NIELSYEVVD KDSIRSGGPV VVLVQLEREE EVTGPVIAPL FPQKREEGWW VVIGDAKSNS LISIK RLTL QQKAKVKLDF VAPATGAHNY TLYFMSDAYM GCDQEYKFSV DVKEAETDSD SD

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Macromolecule #4: U5 small nuclear ribonucleoprotein 40 kDa protein

MacromoleculeName: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 39.359492 KDa
SequenceString: MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC ...String:
MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

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Macromolecule #5: U4/U6 small nuclear ribonucleoprotein Prp3

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 77.669188 KDa
SequenceString: MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKR ELKEVFGDDS EISKESSGVK KRRIPRFEEV EEEPEVIPGP PSESPGMLTK LQIKQMMEAA TRQIEERKKQ L SFISPPTP ...String:
MALSKRELDE LKPWIEKTVK RVLGFSEPTV VTAALNCVGK GMDKKKAADH LKPFLDDSTL RFVDKLFEAV EEGRSSRHSK SSSDRSRKR ELKEVFGDDS EISKESSGVK KRRIPRFEEV EEEPEVIPGP PSESPGMLTK LQIKQMMEAA TRQIEERKKQ L SFISPPTP QPKTPSSSQP ERLPIGNTIQ PSQAATFMND AIEKARKAAE LQARIQAQLA LKPGLIGNAN MVGLANLHAM GI APPKVEL KDQTKPTPLI LDEQGRTVDA TGKEIELTHR MPTLKANIRA VKREQFKQQL KEKPSEDMES NTFFDPRVSI APS QRQRRT FKFHDKGKFE KIAQRLRTKA QLEKLQAEIS QAARKTGIHT STRLALIAPK KELKEGDIPE IEWWDSYIIP NGFD LTEEN PKREDYFGIT NLVEHPAQLN PPVDNDTPVT LGVYLTKKEQ KKLRRQTRRE AQKELQEKVR LGLMPPPEPK VRISN LMRV LGTEAVQDPT KVEAHVRAQM AKRQKAHEEA NAARKLTAEQ RKVKKIKKLK EDISQGVHIS VYRVRNLSNP AKKFKI EAN AGQLYLTGVV VLHKDVNVVV VEGGPKAQKK FKRLMLHRIK WDEQTSNTKG DDDEESDEEA VKKTNKCVLV WEGTAKD RS FGEMKFKQCP TENMAREHFK KHGAEHYWDL ALSESVLEST D

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Macromolecule #6: U4/U6 small nuclear ribonucleoprotein Prp4

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp4 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 58.40798 KDa
SequenceString: MASSRASSTA TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL GKDGLKAGIE AGNINITSGE VFEIEEHISE RQAEVLAEF ERRKRARQIN VSTDDSEVKA CLRALGEPIT LFGEGPAERR ERLRNILSVV GTDALKKTKK DDEKSKKSKE E YQQTWYHE ...String:
MASSRASSTA TKTKAPDDLV APVVKKPHIY YGSLEEKERE RLAKGESGIL GKDGLKAGIE AGNINITSGE VFEIEEHISE RQAEVLAEF ERRKRARQIN VSTDDSEVKA CLRALGEPIT LFGEGPAERR ERLRNILSVV GTDALKKTKK DDEKSKKSKE E YQQTWYHE GPNSLKVARL WIANYSLPRA MKRLEEARLH KEIPETTRTS QMQELHKSLR SLNNFCSQIG DDRPISYCHF SP NSKMLAT ACWSGLCKLW SVPDCNLLHT LRGHNTNVGA IVFHPKSTVS LDPKDVNLAS CAADGSVKLW SLDSDEPVAD IEG HTVRVA RVMWHPSGRF LGTTCYDRSW RLWDLEAQEE ILHQEGHSMG VYDIAFHQDG SLAGTGGLDA FGRVWDLRTG RCIM FLEGH LKEIYGINFS PNGYHIATGS GDNTCKVWDL RQRRCVYTIP AHQNLVTGVK FEPIHGNFLL TGAYDNTAKI WTHPG WSPL KTLAGHEGKV MGLDISSDGQ LIATCSYDRT FKLWMAE

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Macromolecule #7: Pre-mRNA-processing factor 6

MacromoleculeName: Pre-mRNA-processing factor 6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 107.092242 KDa
SequenceString: MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLF SSGPYEKDDE EADAIYAALD KRMDERRKER REQREKEEIE KYRMERPKIQ QQFSDLKRKL AEVTEEEWLS I PEVGDARN ...String:
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLF SSGPYEKDDE EADAIYAALD KRMDERRKER REQREKEEIE KYRMERPKIQ QQFSDLKRKL AEVTEEEWLS I PEVGDARN KRQRNPRYEK LTPVPDSFFA KHLQTGENHT SVDPRQTQFG GLNTPYPGGL NTPYPGGMTP GLMTPGTGEL DM RKIGQAR NTLMDMRLSQ VSDSVSGQTV VDPKGYLTDL NSMIPTHGGD INDIKKARLL LKSVRETNPH HPPAWIASAR LEE VTGKLQ VARNLIMKGT EMCPKSEDVW LEAARLQPGD TAKAVVAQAV RHLPQSVRIY IRAAELETDI RAKKRVLRKA LEHV PNSVR LWKAAVELEE PEDARIMLSR AVECCPTSVE LWLALARLET YENARKVLNK ARENIPTDRH IWITAAKLEE ANGNT QMVE KIIDRAITSL RANGVEINRE QWIQDAEECD RAGSVATCQA VMRAVIGIGI EEEDRKHTWM EDADSCVAHN ALECAR AIY AYALQVFPSK KSVWLRAAYF EKNHGTRESL EALLQRAVAH CPKAEVLWLM GAKSKWLAGD VPAARSILAL AFQANPN SE EIWLAAVKLE SENDEYERAR RLLAKARSSA PTARVFMKSV KLEWVQDNIR AAQDLCEEAL RHYEDFPKLW MMKGQIEE Q KEMMEKAREA YNQGLKKCPH STPLWLLLSR LEEKIGQLTR ARAILEKSRL KNPKNPGLWL ESVRLEYRAG LKNIANTLM AKALQECPNS GILWSEAIFL EARPQRRTKS VDALKKCEHD PHVLLAVAKL FWSQRKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHG TEEQQEEVRK RCESAEPRHG ELWCAVSKDI ANWQKKIGDI LRLVAGRIKN TF

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Macromolecule #8: U4/U6 small nuclear ribonucleoprotein Prp31

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein Prp31 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 55.528969 KDa
SequenceString: MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKT IAKLWDSKMF AEIMMKIEEY ISKQAKASEV MGPVEAAPE YRVIVDANNL TVEIENELNI IHKFIRDKYS KRFPELESLV PNALDYIRTV KELGNSLDKC KNNENLQQIL T NATIMVVS ...String:
MSLADELLAD LEEAAEEEEG GSYGEEEEEP AIEDVQEETQ LDLSGDSVKT IAKLWDSKMF AEIMMKIEEY ISKQAKASEV MGPVEAAPE YRVIVDANNL TVEIENELNI IHKFIRDKYS KRFPELESLV PNALDYIRTV KELGNSLDKC KNNENLQQIL T NATIMVVS VTASTTQGQQ LSEEELERLE EACDMALELN ASKHRIYEYV ESRMSFIAPN LSIIIGASTA AKIMGVAGGL TN LSKMPAC NIMLLGAQRK TLSGFSSTSV LPHTGYIYHS DIVQSLPPDL RRKAARLVAA KCTLAARVDS FHESTEGKVG YEL KDEIER KFDKWQEPPP VKQVKPLPAP LDGQRKKRGG RRYRKMKERL GLTEIRKQAN RMSFGEIEED AYQEDLGFSL GHLG KSGSG RVRQTQVNEA TKARISKTLQ RTLQKQSVVY GGKSTIRDRS SGTASSVAFT PLQGLEIVNP QAAEKKVAEA NQKYF SSMA EFLKVKGEKS GLMST

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Macromolecule #9: Pre-mRNA-splicing factor 38A

MacromoleculeName: Pre-mRNA-splicing factor 38A / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 37.563863 KDa
SequenceString: MANRTVKDAH SIHGTNPQYL VEKIIRTRIY ESKYWKEECF GLTAELVVDK AMELRFVGGV YGGNIKPTPF LCLTLKMLQI QPEKDIIVE FIKNEDFKYV RMLGALYMRL TGTAIDCYKY LEPLYNDYRK IKSQNRNGEF ELMHVDEFID ELLHSERVCD I ILPRLQKR ...String:
MANRTVKDAH SIHGTNPQYL VEKIIRTRIY ESKYWKEECF GLTAELVVDK AMELRFVGGV YGGNIKPTPF LCLTLKMLQI QPEKDIIVE FIKNEDFKYV RMLGALYMRL TGTAIDCYKY LEPLYNDYRK IKSQNRNGEF ELMHVDEFID ELLHSERVCD I ILPRLQKR YVLEEAEQLE PRVSALEEDM DDVESSEEEE EEDEKLERVP SPDHRRRSYR DLDKPRRSPT LRYRRSRSRS PR RRSRSPK RRSPSPRRER HRSKSPRRHR SRSRDRRHRS RSKSPGHHRS HRHRSHSKSP ERSKKSHKKS RRGNE

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Macromolecule #10: Thioredoxin-like protein 4A

MacromoleculeName: Thioredoxin-like protein 4A / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 16.807346 KDa
SequenceString:
MSYMLPHLHN GWQVDQAILS EEDRVVVIRF GHDWDPTCMK MDEVLYSIAE KVKNFAVIYL VDITEVPDFN KMYELYDPCT VMFFFRNKH IMIDLGTGNN NKINWAMEDK QEMVDIIETV YRGARKGRGL VVSPKDYSTK YRY

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Macromolecule #11: Microfibrillar-associated protein 1

MacromoleculeName: Microfibrillar-associated protein 1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 52.050527 KDa
SequenceString: MSVPSALMKQ PPIQSTAGAV PVRNEKGEIS MEKVKVKRYV SGKRPDYAPM ESSDEEDEEF QFIKKAKEQE AEPEEQEEDS SSDPRLRRL QNRISEDVEE RLARHRKIVE PEVVGESDSE VEGDAWRMER EDSSEEEEEE IDDEEIERRR GMMRQRAQER K NEEMEVME ...String:
MSVPSALMKQ PPIQSTAGAV PVRNEKGEIS MEKVKVKRYV SGKRPDYAPM ESSDEEDEEF QFIKKAKEQE AEPEEQEEDS SSDPRLRRL QNRISEDVEE RLARHRKIVE PEVVGESDSE VEGDAWRMER EDSSEEEEEE IDDEEIERRR GMMRQRAQER K NEEMEVME VEDEGRSGEE SESESEYEEY TDSEDEMEPR LKPVFIRKKD RVTVQEREAE ALKQKELEQE AKRMAEERRK YT LKIVEEE TKKELEENKR SLAALDALNT DDENDEEEYE AWKVRELKRI KRDREDREAL EKEKAEIERM RNLTEEERRA ELR ANGKVI TNKAVKGKYK FLQKYYHRGA FFMDEDEEVY KRDFSAPTLE DHFNKTILPK VMQVKNFGRS GRTKYTHLVD QDTT SFDSA WGQESAQNTK FFKQKAAGVR DVFERPSAKK RKTT

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Macromolecule #12: WD40 repeat-containing protein SMU1

MacromoleculeName: WD40 repeat-containing protein SMU1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 57.616742 KDa
SequenceString: MSIEIESSDV IRLIMQYLKE NSLHRALATL QEETTVSLNT VDSIESFVAD INSGHWDTVL QAIQSLKLPD KTLIDLYEQV VLELIELRE LGAARSLLRQ TDPMIMLKQT QPERYIHLEN LLARSYFDPR EAYPDGSSKE KRRAAIAQAL AGEVSVVPPS R LMALLGQA ...String:
MSIEIESSDV IRLIMQYLKE NSLHRALATL QEETTVSLNT VDSIESFVAD INSGHWDTVL QAIQSLKLPD KTLIDLYEQV VLELIELRE LGAARSLLRQ TDPMIMLKQT QPERYIHLEN LLARSYFDPR EAYPDGSSKE KRRAAIAQAL AGEVSVVPPS R LMALLGQA LKWQQHQGLL PPGMTIDLFR GKAAVKDVEE EKFPTQLSRH IKFGQKSHVE CARFSPDGQY LVTGSVDGFI EV WNFTTGK IRKDLKYQAQ DNFMMMDDAV LCMCFSRDTE MLATGAQDGK IKVWKIQSGQ CLRRFERAHS KGVTCLSFSK DSS QILSAS FDQTIRIHGL KSGKTLKEFR GHSSFVNEAT FTQDGHYIIS ASSDGTVKIW NMKTTECSNT FKSLGSTAGT DITV NSVIL LPKNPEHFVV CNRSNTVVIM NMQGQIVRSF SSGKREGGDF VCCALSPRGE WIYCVGEDFV LYCFSTVTGK LERTL TVHE KDVIGIAHHP HQNLIATYSE DGLLKLWKP

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Macromolecule #13: Peptidyl-prolyl cis-trans isomerase H

MacromoleculeName: Peptidyl-prolyl cis-trans isomerase H / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 19.230117 KDa
SequenceString:
MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVAS IYRGPFADEN FKLRHSAPGL LSMANSGPST NGCQFFITCS KCDWLDGKHV VFGKIIDGLL VMRKIENVPT G PNNKPKLP VVISQCGEM

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Macromolecule #14: Zinc finger matrin-type protein 2

MacromoleculeName: Zinc finger matrin-type protein 2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 23.664047 KDa
SequenceString: MASGSGTKNL DFRRKWDKDE YEKLAEKRLT EEREKKDGKP VQPVKRELLR HRDYKVDLES KLGKTIVITK TTPQSEMGGY YCNVCDCVV KDSINFLDHI NGKKHQRNLG MSMRVERSTL DQVKKRFEVN KKKMEEKQKD YDFEERMKEL REEEEKAKAY K KEKQKEKK ...String:
MASGSGTKNL DFRRKWDKDE YEKLAEKRLT EEREKKDGKP VQPVKRELLR HRDYKVDLES KLGKTIVITK TTPQSEMGGY YCNVCDCVV KDSINFLDHI NGKKHQRNLG MSMRVERSTL DQVKKRFEVN KKKMEEKQKD YDFEERMKEL REEEEKAKAY K KEKQKEKK RRAEEDLTFE EDDEMAAVMG FSGFGSTKKS Y

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Macromolecule #15: NHP2-like protein 1

MacromoleculeName: NHP2-like protein 1 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 14.191524 KDa
SequenceString:
MTEADVNPKA YPLADAHLTK KLLDLVQQSC NYKQLRKGAN EATKTLNRGI SEFIVMAADA EPLEIILHLP LLCEDKNVPY VFVRSKQAL GRACGVSRPV IACSVTIKEG SQLKQQIQSI QQSIERLLV

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Macromolecule #16: U4/U6.U5 tri-snRNP-associated protein 1

MacromoleculeName: U4/U6.U5 tri-snRNP-associated protein 1 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 90.414117 KDa
SequenceString: MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPS ERRVKREKRD DGYEAAASSK TSSGDASSLS IEETNKLRAK LGLKPLEVNA IKKEAGTKEE PVTADVINPM A LRQREELR ...String:
MGSSKKHRGE KEAAGTTAAA GTGGATEQPP RHREHKKHKH RSGGSGGSGG ERRKRSRERG GERGSGRRGA EAEARSSTHG RERSQAEPS ERRVKREKRD DGYEAAASSK TSSGDASSLS IEETNKLRAK LGLKPLEVNA IKKEAGTKEE PVTADVINPM A LRQREELR EKLAAAKEKR LLNQKLGKIK TLGEDDPWLD DTAAWIERSR QLQKEKDLAE KRAKLLEEMD QEFGVSTLVE EE FGQRRQD LYSARDLQGL TVEHAIDSFR EGETMILTLK DKGVLQEEED VLVNVNLVDK ERAEKNVELR KKKPDYLPYA EDE SVDDLA QQKPRSILSK YDEELEGERP HSFRLEQGGT ADGLRERELE EIRAKLRLQA QSLSTVGPRL ASEYLTPEEM VTFK KTKRR VKKIRKKEKE VVVRADDLLP LGDQTQDGDF GSRLRGRGRR RVSEVEEEKE PVPQPLPSDD TRVENMDISD EEEGG APPP GSPQVLEEDE AELELQKQLE KGRRLRQLQQ LQQLRDSGEK VVEIVKKLES RQRGWEEDED PERKGAIVFN ATSEFC RTL GEIPTYGLAG NREEQEELMD FERDEERSAN GGSESDGEEN IGWSTVNLDE EKQQQDFSAS STTILDEEPI VNRGLAA AL LLCQNKGLLE TTVQKVARVK APNKSLPSAV YCIEDKMAID DKYSRREEYR GFTQDFKEKD GYKPDVKIEY VDETGRKL T PKEAFRQLSH RFHGKGSGKM KTERRMKKLD EEALLKKMSS SDTPLGTVAL LQEKQKAQKT PYIVLSGSGK SMNANTITK

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Macromolecule #17: WW domain-binding protein 4

MacromoleculeName: WW domain-binding protein 4 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 42.575801 KDa
SequenceString: MADYWKSQPK KFCDYCKCWI ADNRPSVEFH ERGKNHKENV AKRISEIKQK SLDKAKEEEK ASKEFAAMEA AALKAYQEDL KRLGLESEI LEPSITPVTS TIPPTSTSNQ QKEKKEKKKR KKDPSKGRWV EGITSEGYHY YYDLISGASQ WEKPEGFQGD L KKTAVKTV ...String:
MADYWKSQPK KFCDYCKCWI ADNRPSVEFH ERGKNHKENV AKRISEIKQK SLDKAKEEEK ASKEFAAMEA AALKAYQEDL KRLGLESEI LEPSITPVTS TIPPTSTSNQ QKEKKEKKKR KKDPSKGRWV EGITSEGYHY YYDLISGASQ WEKPEGFQGD L KKTAVKTV WVEGLSEDGF TYYYNTETGE SRWEKPDDFI PHTSDLPSSK VNENSLGTLD ESKSSDSHSD SDGEQEAEEG GV STETEKP KIKFKEKNKN SDGGSDPETQ KEKSIQKQNS LGSNEEKSKT LKKSNPYGEW QEIKQEVESH EEVDLELPST ENE YVSTSE ADGGGEPKVV FKEKTVTSLG VMADGVAPVF KKRRTENGKS RNLRQRGDDQ

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Macromolecule #18: Protein Red

MacromoleculeName: Protein Red / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 65.731508 KDa
SequenceString: MPERDSEPFS NPLAPDGHDV DDPHSFHQSK LTNEDFRKLL MTPRAAPTSA PPSKSRHHEM PREYNEDEDP AARRRKKKSY YAKLRQQEI ERERELAEKY RDRAKERRDG VNKDYEETEL ISTTANYRAV GPTAEADKSA AEKRRQLIQE SKFLGGDMEH T HLVKGLDF ...String:
MPERDSEPFS NPLAPDGHDV DDPHSFHQSK LTNEDFRKLL MTPRAAPTSA PPSKSRHHEM PREYNEDEDP AARRRKKKSY YAKLRQQEI ERERELAEKY RDRAKERRDG VNKDYEETEL ISTTANYRAV GPTAEADKSA AEKRRQLIQE SKFLGGDMEH T HLVKGLDF ALLQKVRAEI ASKEKEEEEL MEKPQKETKK DEDPENKIEF KTRLGRNVYR MLFKSKAYER NELFLPGRMA YV VDLDDEY ADTDIPTTLI RSKADCPTME AQTTLTTNDI VISKLTQILS YLRQGTRNKK LKKKDKGKLE EKKPPEADMN IFE DIGDYV PSTTKTPRDK ERERYRERER DRERDRDRDR ERERERDRER ERERDREREE EKKRHSYFEK PKVDDEPMDV DKGP GSTKE LIKSINEKFA GSAGWEGTES LKKPEDKKQL GDFFGMSNSY AECYPATMDD MAVDSDEEVD YSKMDQGNKK GPLGR WDFD TQEEYSEYMN NKEALPKAAF QYGIKMSEGR KTRRFKETND KAELDRQWKK ISAIIEKRKK MEADGVEVKR PKY

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Macromolecule #19: Small nuclear ribonucleoprotein Sm D2

MacromoleculeName: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 19 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 13.551928 KDa
SequenceString:
MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

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Macromolecule #20: Small nuclear ribonucleoprotein F

MacromoleculeName: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 20 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 9.734171 KDa
SequenceString:
MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

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Macromolecule #21: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 21 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.817601 KDa
SequenceString:
MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQS VSN

+
Macromolecule #22: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 22 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 8.508084 KDa
SequenceString:
MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV

+
Macromolecule #23: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 23 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 13.940308 KDa
SequenceString:
MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNK NQGSGAGRGK AAILKAQVAA RGRGRGMGRG NIFQKRR

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Macromolecule #24: Small nuclear ribonucleoprotein-associated proteins B and B'

MacromoleculeName: Small nuclear ribonucleoprotein-associated proteins B and B'
type: protein_or_peptide / ID: 24 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 24.642131 KDa
SequenceString: MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY ...String:
MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY PPGRGGPPPP MGRGAPPPGM MGPPPGMRPP MGPPMGIPPG RGTPMGMPPP GMRPPPPGMR GPPPPGMRPP RP

+
Macromolecule #25: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 25 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 13.310653 KDa
SequenceString:
MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKS KKREAVAGRG RGRGRGRGRG RGRGRGGPRR

+
Macromolecule #26: U6 snRNA-associated Sm-like protein LSm2

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm2 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.847495 KDa
SequenceString:
MLFYSFFKSL VGKDVVVELK NDLSICGTLH SVDQYLNIKL TDISVTDPEK YPHMLSVKNC FIRGSVVRYV QLPADEVDTQ LLQDAARKE ALQQKQ

+
Macromolecule #27: U6 snRNA-associated Sm-like protein LSm3

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm3 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.85939 KDa
SequenceString:
MADDVDQQQT TNTVEEPLDL IRLSLDERIY VKMRNDRELR GRLHAYDQHL NMILGDVEET VTTIEIDEET YEEIYKSTKR NIPMLFVRG DGVVLVAPPL RVG

+
Macromolecule #28: U6 snRNA-associated Sm-like protein LSm4

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm4 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 15.375775 KDa
SequenceString:
MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRG RGGLQQQKQQ KGRGMGGAGR GVFGGRGRGG IPGTGRGQPE KKPGRQAGKQ

+
Macromolecule #29: U6 snRNA-associated Sm-like protein LSm5

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm5 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 9.945448 KDa
SequenceString:
MAANATTNPS QLLPLELVDK CIGSRIHIVM KSDKEIVGTL LGFDDFVNMV LEDVTEFEIT PEGRRITKLD QILLNGNNIT MLVPGGEGP EV

+
Macromolecule #30: U6 snRNA-associated Sm-like protein LSm6

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm6 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 9.139571 KDa
SequenceString:
MSLRKQTPSD FLKQIIGRPV VVKLNSGVDY RGVLACLDGY MNIALEQTEE YVNGQLKNKY GDAFIRGNNV LYISTQKRRM

+
Macromolecule #31: U6 snRNA-associated Sm-like protein LSm7

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm7 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 11.617363 KDa
SequenceString:
MADKEKKKKE SILDLSKYID KTIRVKFQGG REASGILKGF DPLLNLVLDG TIEYMRDPDD QYKLTEDTRQ LGLVVCRGTS VVLICPQDG MEAIPNPFIQ QQDA

+
Macromolecule #32: U6 snRNA-associated Sm-like protein LSm8

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm8 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.410589 KDa
SequenceString:
MTSALENYIN RTVAVITSDG RMIVGTLKGF DQTINLILDE SHERVFSSSQ GVEQVVLGLY IVRGDNVAVI GEIDEETDSA LDLGNIRAE PLNSVAH

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Macromolecule #33: Splicing factor 3B subunit 1

MacromoleculeName: Splicing factor 3B subunit 1 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 146.024938 KDa
SequenceString: MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR FAGYVTSIAA TELEDDDDDY SSSTSLLGQK KPGYHAPVA LLNDIPQSTE QYDPFAEHRP PKIADREDEY KKHRRTMIIS PERLDPFADG GKTPDPKMNA RTYMDVMREQ H LTKEEREI ...String:
MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR FAGYVTSIAA TELEDDDDDY SSSTSLLGQK KPGYHAPVA LLNDIPQSTE QYDPFAEHRP PKIADREDEY KKHRRTMIIS PERLDPFADG GKTPDPKMNA RTYMDVMREQ H LTKEEREI RQQLAEKAKA GELKVVNGAA ASQPPSKRKR RWDQTADQTP GATPKKLSSW DQAETPGHTP SLRWDETPGR AK GSETPGA TPGSKIWDPT PSHTPAGAAT PGRGDTPGHA TPGHGGATSS ARKNRWDETP KTERDTPGHG SGWAETPRTD RGG DSIGET PTPGASKRKS RWDETPASQM GGSTPVLTPG KTPIGTPAMN MATPTPGHIM SMTPEQLQAW RWEREIDERN RPLS DEELD AMFPEGYKVL PPPAGYVPIR TPARKLTATP TPLGGMTGFH MQTEDRTMKS VNDQPSGNLP FLKPDDIQYF DKLLV DVDE STLSPEEQKE RKIMKLLLKI KNGTPPMRKA ALRQITDKAR EFGAGPLFNQ ILPLLMSPTL EDQERHLLVK VIDRIL YKL DDLVRPYVHK ILVVIEPLLI DEDYYARVEG REIISNLAKA AGLATMISTM RPDIDNMDEY VRNTTARAFA VVASALG IP SLLPFLKAVC KSKKSWQARH TGIKIVQQIA ILMGCAILPH LRSLVEIIEH GLVDEQQKVR TISALAIAAL AEAATPYG I ESFDSVLKPL WKGIRQHRGK GLAAFLKAIG YLIPLMDAEY ANYYTREVML ILIREFQSPD EEMKKIVLKV VKQCCGTDG VEANYIKTEI LPPFFKHFWQ HRMALDRRNY RQLVDTTVEL ANKVGAAEII SRIVDDLKDE AEQYRKMVME TIEKIMGNLG AADIDHKLE EQLIDGILYA FQEQTTEDSV MLNGFGTVVN ALGKRVKPYL PQICGTVLWR LNNKSAKVRQ QAADLISRTA V VMKTCQEE KLMGHLGVVL YEYLGEEYPE VLGSILGALK AIVNVIGMHK MTPPIKDLLP RLTPILKNRH EKVQENCIDL VG RIADRGA EYVSAREWMR ICFELLELLK AHKKAIRRAT VNTFGYIAKA IGPHDVLATL LNNLKVQERQ NRVCTTVAIA IVA ETCSPF TVLPALMNEY RVPELNVQNG VLKSLSFLFE YIGEMGKDYI YAVTPLLEDA LMDRDLVHRQ TASAVVQHMS LGVY GFGCE DSLNHLLNYV WPNVFETSPH VIQAVMGALE GLRVAIGPCR MLQYCLQGLF HPARKVRDVY WKIYNSIYIG SQDAL IAHY PRIYNDDKNT YIRYELDYIL

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Macromolecule #34: Splicing factor 3B subunit 3 (SF3B3)

MacromoleculeName: Splicing factor 3B subunit 3 (SF3B3) / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 135.718844 KDa
SequenceString: MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY ...String:
MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY HVVGVDVGFE NPMFACLEMD YEEADNDPTG EAAANTQQTL TFYELDLGLN HVVRKYSEPL EEHGNFLITV PG GSDGPSG VLICSENYIT YKNFGDQPDI RCPIPRRRND LDDPERGMIF VCSATHKTKS MFFFLAQTEQ GDIFKITLET DED MVTEIR LKYFDTVPVA AAMCVLKTGF LFVASEFGNH YLYQIAHLGD DDEEPEFSSA MPLEEGDTFF FQPRPLKNLV LVDE LDSLS PILFCQIADL ANEDTPQLYV ACGRGPRSSL RVLRHGLEVS EMAVSELPGN PNAVWTVRRH IEDEFDAYII VSFVN ATLV LSIGETVEEV TDSGFLGTTP TLSCSLLGDD ALVQVYPDGI RHIRADKRVN EWKTPGKKTI VKCAVNQRQV VIALTG GEL VYFEMDPSGQ LNEYTERKEM SADVVCMSLA NVPPGEQRSR FLAVGLVDNT VRIISLDPSD CLQPLSMQAL PAQPESL CI VEMGGTEKQD ELGERGSIGF LYLNIGLQNG VLLRTVLDPV TGDLSDTRTR YLGSRPVKLF RVRMQGQEAV LAMSSRSW L SYSYQSRFHL TPLSYETLEF ASGFASEQCP EGIVAISTNT LRILALEKLG AVFNQVAFPL QYTPRKFVIH PESNNLIII ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA AFLNENLPES IFGAPKAGNG QWASVIRVMN PIQGNTLDLV QLEQNEAAF SVAVCRFSNT GEDWYVLVGV AKDLILNPRS VAGGFVYTYK LVNNGEKLEF LHKTPVEEVP AAIAPFQGRV L IGVGKLLR VYDLGKKKLL RKCENKHIAN YISGIQTIGH RVIVSDVQES FIWVRYKRNE NQLIIFADDT YPRWVTTASL LD YDTVAGA DKFGNICVVR LPPNTNDEVD EDPTGNKALW DRGLLNGASQ KAEVIMNYHV GETVLSLQKT TLIPGGSESL VYT TLSGGI GILVPFTSHE DHDFFQHVEM HLRSEHPPLC GRDHLSFRSY YFPVKNVIDG DLCEQFNSME PNKQKNVSEE LDRT PPEVS KKLEDIRTRY AF

+
Macromolecule #35: Splicing factor 3B subunit 5

MacromoleculeName: Splicing factor 3B subunit 5 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 10.149369 KDa
SequenceString:
MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL NYFAIAENES KARVRFNLME KMLQPCGPPA DKPEEN

+
Macromolecule #36: PHD finger-like domain-containing protein 5A

MacromoleculeName: PHD finger-like domain-containing protein 5A / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 12.427524 KDa
SequenceString:
MAKHHPDLIF CRKQAGVAIG RLCEKCDGKC VICDSYVRPC TLVRICDECN YGSYQGRCVI CGGPGVSDAY YCKECTIQEK DRDGCPKIV NLGSSKTDLF YERKKYGFKK R

+
Macromolecule #37: U2 small nuclear ribonucleoprotein A'

MacromoleculeName: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 28.631766 KDa
SequenceString: MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPD LTELILTNNS LVELGDLDPL ASLKSLTYLC ILRNPVTNKK HYRLYVIYKV PQVRVLDFQK VKFLKERQEA E KMFKGKRG ...String:
MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPD LTELILTNNS LVELGDLDPL ASLKSLTYLC ILRNPVTNKK HYRLYVIYKV PQVRVLDFQK VKFLKERQEA E KMFKGKRG AQLAKDIARR SKTFNPGAGL PTDKKKGGPS PGDVEAIKNA IANASTLAEV ERLKGLLQSG QIPGRERRSG PT DDGEEEM EEDTVTNGS

+
Macromolecule #38: U2 small nuclear ribonucleoprotein B''

MacromoleculeName: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 25.524367 KDa
SequenceString: MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSD IISKMRGTFA DKEKKKEKKK AKTVEQTATT TNKKPGQGTP NSANTQGNST PNPQVPDYPP NYILFLNNLP E ETNEMMLS ...String:
MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSD IISKMRGTFA DKEKKKEKKK AKTVEQTATT TNKKPGQGTP NSANTQGNST PNPQVPDYPP NYILFLNNLP E ETNEMMLS MLFNQFPGFK EVRLVPGRHD IAFVEFENDG QAGAARDALQ GFKITPSHAM KITYAKK

+
Macromolecule #39: MINX pre-mRNA

MacromoleculeName: MINX pre-mRNA / type: rna / ID: 39 / Number of copies: 1
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 103.979148 KDa
SequenceString: GGGAGACGGA AUUCGAGCUC GCCCACUCUU GGAUCGGAAA CCCGUCGGCC UCCGAACGGU AAGAGCCUAG CAUGUAGAAC UGGUUACCU GCAGCCCAAG CUUGCUGCAC GUCUAGGGCG CAGUAGUCCA GGGUUUCCUU GAUGAUGUCA UACUUAUCCU G UCCCUUUU ...String:
GGGAGACGGA AUUCGAGCUC GCCCACUCUU GGAUCGGAAA CCCGUCGGCC UCCGAACGGU AAGAGCCUAG CAUGUAGAAC UGGUUACCU GCAGCCCAAG CUUGCUGCAC GUCUAGGGCG CAGUAGUCCA GGGUUUCCUU GAUGAUGUCA UACUUAUCCU G UCCCUUUU UUUUCCACAG CUCGCGGUUG AGGACAAACU CUUCGCGGUC UUUCCAGUGG GGAUCCGAUA UCCGUACACC AU CAGGGUA CGAGCUAGCC CAUGGCGUAC ACCAUCAGGG UACGACUAGU AGAUCUCGUA CACCAUCAGG GUACGGAAUU CUC UAGA

+
Macromolecule #40: Human gene for small nuclear RNA U2 (snRNA U2)

MacromoleculeName: Human gene for small nuclear RNA U2 (snRNA U2) / type: rna / ID: 40 / Number of copies: 1
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 60.186445 KDa
SequenceString:
AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUGGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

+
Macromolecule #41: Homo sapiens U4A snRNA

MacromoleculeName: Homo sapiens U4A snRNA / type: rna / ID: 41 / Number of copies: 1
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 46.528465 KDa
SequenceString:
AGCUUUGCGC AGUGGCAGUA UCGUAGCCAA UGAGGUUUAU CCGAGGCGCG AUUAUUGCUA AUUGAAAACU UUUCCCAAUA CCCCGCCGU GACGACUUGC AAUAUAGUCG GCAUUGGCAA UUUUUGACAG UCUCUACGGA GACUGG

+
Macromolecule #42: Homo sapiens U5 A small nuclear RNA

MacromoleculeName: Homo sapiens U5 A small nuclear RNA / type: rna / ID: 42 / Number of copies: 1
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 36.908668 KDa
SequenceString:
AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGCC UUGCCUUGGC AAGGCUA

+
Macromolecule #43: Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA

MacromoleculeName: Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA
type: rna / ID: 43 / Number of copies: 1
Source (natural)Organism: Human (human)
Molecular weightTheoretical: 34.09827 KDa
SequenceString:
GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.9
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 277 K

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Sampling interval: 14.0 µm / Digitization - Frames/image: 2-17 / Average exposure time: 0.05 sec. / Average electron dose: 1.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 0.01 mm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

DetailsFALCON III Direct Electron Detector
Startup modelType of model: OTHER / Details: Reconstruction from negatively stained particles
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 44629
Initial angle assignmentType: COMMON LINE
Final angle assignmentType: OTHER

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Atomic model buiding 1

RefinementSpace: REAL
Output model

PDB-5o9z:
Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex)

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