+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37418 | |||||||||
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Title | Cryo-EM structure of the ABCG25 bound to CHS | |||||||||
Map data | ||||||||||
Sample |
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Keywords | exporter / complex / transporter / MEMBRANE PROTEIN | |||||||||
Function / homology | Function and homology information intercellular transport / negative regulation of post-embryonic development / abscisic acid transport / export from cell / response to abscisic acid / abscisic acid-activated signaling pathway / efflux transmembrane transporter activity / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / response to cold ...intercellular transport / negative regulation of post-embryonic development / abscisic acid transport / export from cell / response to abscisic acid / abscisic acid-activated signaling pathway / efflux transmembrane transporter activity / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / response to cold / transmembrane transport / response to heat / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Arabidopsis thaliana (thale cress) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
Authors | Xin J / Yan KG | |||||||||
Funding support | China, 1 items
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Citation | Journal: Plant Commun / Year: 2024 Title: Structural insights into AtABCG25, an angiosperm-specific abscisic acid exporter. Authors: Jian Xin / Yeling Zhou / Yichun Qiu / He Geng / Yuzhu Wang / Yi Song / Jiansheng Liang / Kaige Yan / Abstract: Cellular hormone homeostasis is essential for precise spatial and temporal signaling responses and plant fitness. Abscisic acid (ABA) plays pivotal roles in orchestrating various developmental and ...Cellular hormone homeostasis is essential for precise spatial and temporal signaling responses and plant fitness. Abscisic acid (ABA) plays pivotal roles in orchestrating various developmental and stress responses and confers fitness benefits over ecological and evolutionary timescales in terrestrial plants. Cellular ABA level is regulated by complex processes, including biosynthesis, catabolism, and transport. AtABCG25 is the first ABA exporter identified through genetic screening and affects diverse ABA responses. Resolving the structural basis of ABA export by ABCG25 is critical for further manipulations of ABA homeostasis and plant fitness. We used cryo-electron microscopy to elucidate the structural dynamics of AtABCG25 and successfully characterized different states, including apo AtABCG25, ABA-bound AtABCG25, and ATP-bound AtABCG25 (E232Q). Notably, AtABCG25 forms a homodimer that features a deep, slit-like cavity in the transmembrane domain, and we precisely characterized the critical residues in the cavity where ABA binds. ATP binding triggers closure of the nucleotide-binding domains and conformational transitions in the transmembrane domains. We show that AtABCG25 belongs to a conserved ABCG subfamily that originated during the evolution of angiosperms. This subfamily neofunctionalized to regulate seed germination via the endosperm, in concert with the evolution of this angiosperm-specific, embryo-nourishing tissue. Collectively, these findings provide valuable insights into the intricate substrate recognition and transport mechanisms of the ABA exporter AtABCG25, paving the way for genetic manipulation of ABA homeostasis and plant fitness. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37418.map.gz | 59.6 MB | EMDB map data format | |
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Header (meta data) | emd-37418-v30.xml emd-37418.xml | 14.3 KB 14.3 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_37418_fsc.xml | 8.4 KB | Display | FSC data file |
Images | emd_37418.png | 73.4 KB | ||
Masks | emd_37418_msk_1.map | 64 MB | Mask map | |
Filedesc metadata | emd-37418.cif.gz | 5.6 KB | ||
Others | emd_37418_half_map_1.map.gz emd_37418_half_map_2.map.gz | 59.1 MB 59.1 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37418 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37418 | HTTPS FTP |
-Validation report
Summary document | emd_37418_validation.pdf.gz | 788.6 KB | Display | EMDB validaton report |
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Full document | emd_37418_full_validation.pdf.gz | 788.1 KB | Display | |
Data in XML | emd_37418_validation.xml.gz | 16.5 KB | Display | |
Data in CIF | emd_37418_validation.cif.gz | 21.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37418 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37418 | HTTPS FTP |
-Related structure data
Related structure data | 8wbaMC 8wamC 8wbxC 8wd6C C: citing same article (ref.) M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_37418.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_37418_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_37418_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_37418_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Homodimer of ABCG25 bound to CHS
Entire | Name: Homodimer of ABCG25 bound to CHS |
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Components |
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-Supramolecule #1: Homodimer of ABCG25 bound to CHS
Supramolecule | Name: Homodimer of ABCG25 bound to CHS / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
-Macromolecule #1: ABC transporter G family member 25
Macromolecule | Name: ABC transporter G family member 25 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Arabidopsis thaliana (thale cress) |
Molecular weight | Theoretical: 77.121148 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MDYKDHDGDY KDHDIDYKDD DDKGSDLEVL FQGPGSMSAF DGVENQMNGP DSSPRLSQDP REPRSLLSSS CFPITLKFVD VCYRVKIHG MSNDSCNIKK LLGLKQKPSD ETRSTEERTI LSGVTGMISP GEFMAVLGPS GSGKSTLLNA VAGRLHGSNL T GKILINDG ...String: MDYKDHDGDY KDHDIDYKDD DDKGSDLEVL FQGPGSMSAF DGVENQMNGP DSSPRLSQDP REPRSLLSSS CFPITLKFVD VCYRVKIHG MSNDSCNIKK LLGLKQKPSD ETRSTEERTI LSGVTGMISP GEFMAVLGPS GSGKSTLLNA VAGRLHGSNL T GKILINDG KITKQTLKRT GFVAQDDLLY PHLTVRETLV FVALLRLPRS LTRDVKLRAA ESVISELGLT KCENTVVGNT FI RGISGGE RKRVSIAHEL LINPSLLVLD EPTSGLDATA ALRLVQTLAG LAHGKGKTVV TSIHQPSSRV FQMFDTVLLL SEG KCLFVG KGRDAMAYFE SVGFSPAFPM NPADFLLDLA NGVCQTDGVT EREKPNVRQT LVTAYDTLLA PQVKTCIEVS HFPQ DNARF VKTRVNGGGI TTCIATWFSQ LCILLHRLLK ERRHESFDLL RIFQVVAASI LCGLMWWHSD YRDVHDRLGL LFFIS IFWG VLPSFNAVFT FPQERAIFTR ERASGMYTLS SYFMAHVLGS LSMELVLPAS FLTFTYWMVY LRPGIVPFLL TLSVLL LYV LASQGLGLAL GAAIMDAKKA STIVTVTMLA FVLTGGYYVN KVPSGMVWMK YVSTTFYCYR LLVAIQYGSG EEILRML GC DSKGKQGASA ATSAGCRFVE EEVIGDVGMW TSVGVLFLMF FGYRVLAYLA LRRIKH UniProtKB: ABC transporter G family member 25 |
-Macromolecule #2: CHOLESTEROL HEMISUCCINATE
Macromolecule | Name: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 2 / Number of copies: 2 / Formula: Y01 |
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Molecular weight | Theoretical: 486.726 Da |
Chemical component information | ChemComp-Y01: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 6.8 mg/mL |
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Buffer | pH: 7 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 281 K |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 1.5625 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |