+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35771 | |||||||||
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Title | Cryo-EM structure of the PEA-bound mTAAR9 complex | |||||||||
Map data | ||||||||||
Sample |
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Keywords | PEA / mTAAR9 / MEMBRANE PROTEIN | |||||||||
Function / homology | Function and homology information Amine ligand-binding receptors / G alpha (s) signalling events / trace-amine receptor activity / plasma membrane Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.17 Å | |||||||||
Authors | Sun JP / Li Q / Yang F / Xu YF / Guo LL / Lian S / Zhang MH / Rong NK | |||||||||
Funding support | China, 1 items
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Citation | Journal: Nature / Year: 2023 Title: Structural basis of amine odorant perception by a mammal olfactory receptor. Authors: Lulu Guo / Jie Cheng / Shuo Lian / Qun Liu / Yan Lu / Yuan Zheng / Kongkai Zhu / Minghui Zhang / Yalei Kong / Chao Zhang / Naikang Rong / Yuming Zhuang / Guoxing Fang / Jingjing Jiang / ...Authors: Lulu Guo / Jie Cheng / Shuo Lian / Qun Liu / Yan Lu / Yuan Zheng / Kongkai Zhu / Minghui Zhang / Yalei Kong / Chao Zhang / Naikang Rong / Yuming Zhuang / Guoxing Fang / Jingjing Jiang / Tianyao Zhang / Xiang Han / Zili Liu / Ming Xia / Shangming Liu / Lei Zhang / Stephen D Liberles / Xiao Yu / Yunfei Xu / Fan Yang / Qian Li / Jin-Peng Sun / Abstract: Odorants are detected as smell in the nasal epithelium of mammals by two G-protein-coupled receptor families, the odorant receptors and the trace amine-associated receptors (TAARs). TAARs emerged ...Odorants are detected as smell in the nasal epithelium of mammals by two G-protein-coupled receptor families, the odorant receptors and the trace amine-associated receptors (TAARs). TAARs emerged following the divergence of jawed and jawless fish, and comprise a large monophyletic family of receptors that recognize volatile amine odorants to elicit both intraspecific and interspecific innate behaviours such as attraction and aversion. Here we report cryo-electron microscopy structures of mouse TAAR9 (mTAAR9) and mTAAR9-G or mTAAR9-G trimers in complex with β-phenylethylamine, N,N-dimethylcyclohexylamine or spermidine. The mTAAR9 structures contain a deep and tight ligand-binding pocket decorated with a conserved DWY motif, which is essential for amine odorant recognition. In the mTAAR9 structure, a unique disulfide bond connecting the N terminus to ECL2 is required for agonist-induced receptor activation. We identify key structural motifs of TAAR family members for detecting monoamines and polyamines and the shared sequence of different TAAR members that are responsible for recognition of the same odour chemical. We elucidate the molecular basis of mTAAR9 coupling to G and G by structural characterization and mutational analysis. Collectively, our results provide a structural basis for odorant detection, receptor activation and G coupling of an amine olfactory receptor. | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35771.map.gz | 16.9 MB | EMDB map data format | |
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Header (meta data) | emd-35771-v30.xml emd-35771.xml | 14.9 KB 14.9 KB | Display Display | EMDB header |
Images | emd_35771.png | 62.4 KB | ||
Filedesc metadata | emd-35771.cif.gz | 5.4 KB | ||
Others | emd_35771_half_map_1.map.gz emd_35771_half_map_2.map.gz | 17 MB 17 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35771 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35771 | HTTPS FTP |
-Validation report
Summary document | emd_35771_validation.pdf.gz | 667.2 KB | Display | EMDB validaton report |
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Full document | emd_35771_full_validation.pdf.gz | 666.8 KB | Display | |
Data in XML | emd_35771_validation.xml.gz | 9.9 KB | Display | |
Data in CIF | emd_35771_validation.cif.gz | 11.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35771 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-35771 | HTTPS FTP |
-Related structure data
Related structure data | 8iwmMC 8itfC 8iw1C 8iw4C 8iw7C 8iw9C 8iweC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_35771.map.gz / Format: CCP4 / Size: 22.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.076 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_35771_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35771_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Cryo-EM structure of the PEA-bound mTAAR9 complex
Entire | Name: Cryo-EM structure of the PEA-bound mTAAR9 complex |
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Components |
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-Supramolecule #1: Cryo-EM structure of the PEA-bound mTAAR9 complex
Supramolecule | Name: Cryo-EM structure of the PEA-bound mTAAR9 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Mus musculus (house mouse) |
-Macromolecule #1: Trace amine-associated receptor 9
Macromolecule | Name: Trace amine-associated receptor 9 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: Mus musculus (house mouse) |
Molecular weight | Theoretical: 38.928238 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MTSDFSPEPP MELCYENVNG SCIKSSYAPW PRAILYGVLG LGALLAVFGN LLVIIAILHF KQLHTPTNFL VASLACADFL VGVTVMPFS TVRSVESCWY FGESYCKFHT CFDTSFCFAS LFHLCCISID RYIAVTDPLT YPTKFTVSVS GLCIALSWFF S VTYSFSIF ...String: MTSDFSPEPP MELCYENVNG SCIKSSYAPW PRAILYGVLG LGALLAVFGN LLVIIAILHF KQLHTPTNFL VASLACADFL VGVTVMPFS TVRSVESCWY FGESYCKFHT CFDTSFCFAS LFHLCCISID RYIAVTDPLT YPTKFTVSVS GLCIALSWFF S VTYSFSIF YTGANEEGIE ELVVALTCVG GCQAPLNQNW VLLCFLLFFL PTVVMVFLYG RIFLVAKYQA RKIEGTANQA QA SSESYKE RVAKRERKAA KTLGIAMAAF LVSWLPYIID AVIDAYMNFI TPAYVYEILV WCVYYNSAMN PLIYAFFYPW FRK AIKLIV SGKVFRADSS TTNLFSEEAG AG UniProtKB: Trace amine-associated receptor 9 |
-Macromolecule #2: 2-PHENYLETHYLAMINE
Macromolecule | Name: 2-PHENYLETHYLAMINE / type: ligand / ID: 2 / Number of copies: 1 / Formula: PEA |
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Molecular weight | Theoretical: 122.188 Da |
Chemical component information | ChemComp-PEA: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.875 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: DIFFRACTION / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: OTHER |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.17 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 463012 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |