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基本情報
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タイトル | Structure of mammalian spectrin-actin junctional complex of membrane skeleton, State I, Global map | |||||||||
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![]() | Macrocomplex / membrane skeleton / spectrin-actin junction / MEMBRANE PROTEIN | |||||||||
機能・相同性 | ![]() NCAM signaling for neurite out-growth / RAF/MAP kinase cascade / negative regulation of protein targeting to membrane / positive regulation of adherens junction organization / pointed-end actin filament capping / Gap junction degradation / Formation of annular gap junctions / Regulation of actin dynamics for phagocytic cup formation / EPHB-mediated forward signaling / Adherens junctions interactions ...NCAM signaling for neurite out-growth / RAF/MAP kinase cascade / negative regulation of protein targeting to membrane / positive regulation of adherens junction organization / pointed-end actin filament capping / Gap junction degradation / Formation of annular gap junctions / Regulation of actin dynamics for phagocytic cup formation / EPHB-mediated forward signaling / Adherens junctions interactions / VEGFA-VEGFR2 Pathway / Cell-extracellular matrix interactions / RHO GTPases Activate WASPs and WAVEs / MAP2K and MAPK activation / UCH proteinases / Clathrin-mediated endocytosis / cuticular plate / Striated Muscle Contraction / spectrin / RHOF GTPase cycle / positive regulation of establishment of endothelial barrier / lymphocyte homeostasis / lens fiber cell development / myofibril assembly / spectrin-associated cytoskeleton / negative regulation of peptidyl-tyrosine phosphorylation / negative regulation of substrate adhesion-dependent cell spreading / porphyrin-containing compound biosynthetic process / plasma membrane organization / platelet dense tubular network membrane / structural constituent of postsynaptic actin cytoskeleton / negative regulation of focal adhesion assembly / cell projection membrane / dense body / regulation of filopodium assembly / COP9 signalosome / actin filament capping / barbed-end actin filament capping / positive regulation of fibroblast migration / RHO GTPases activate IQGAPs / regulation of lamellipodium assembly / RHO GTPases Activate Formins / COPI-mediated anterograde transport / lamellipodium assembly / spectrin binding / cortical cytoskeleton / NuA4 histone acetyltransferase complex / tropomyosin binding / negative regulation of peptidyl-threonine phosphorylation / negative regulation of peptidyl-serine phosphorylation / striated muscle thin filament / actin filament bundle assembly / : / hemopoiesis / positive regulation of blood coagulation / erythrocyte development / positive regulation of T cell proliferation / cellular response to cAMP / cellular response to calcium ion / adult locomotory behavior / axonogenesis / cell projection / muscle contraction / cell motility / actin filament / regulation of actin cytoskeleton organization / actin filament organization / adherens junction / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / structural constituent of cytoskeleton / SH3 domain binding / : / actin filament binding / actin cytoskeleton / positive regulation of protein binding / cell junction / actin binding / regulation of cell shape / cytoplasmic vesicle / actin cytoskeleton organization / protein-containing complex assembly / postsynaptic density / cytoskeleton / hydrolase activity / axon / focal adhesion / signaling receptor binding / synapse / calcium ion binding / protein kinase binding / perinuclear region of cytoplasm / protein-containing complex / ATP binding / membrane / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.6 Å | |||||||||
![]() | Li N / Chen S / Gao N | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structural basis of membrane skeleton organization in red blood cells. 著者: Ningning Li / Siyi Chen / Kui Xu / Meng-Ting He / Meng-Qiu Dong / Qiangfeng Cliff Zhang / Ning Gao / ![]() 要旨: The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the ...The spectrin-based membrane skeleton is a ubiquitous membrane-associated two-dimensional cytoskeleton underneath the lipid membrane of metazoan cells. Mutations of skeleton proteins impair the mechanical strength and functions of the membrane, leading to several different types of human diseases. Here, we report the cryo-EM structures of the native spectrin-actin junctional complex (from porcine erythrocytes), which is a specialized short F-actin acting as the central organizational unit of the membrane skeleton. While an α-/β-adducin hetero-tetramer binds to the barbed end of F-actin as a flexible cap, tropomodulin and SH3BGRL2 together create an absolute cap at the pointed end. The junctional complex is strengthened by ring-like structures of dematin in the middle actin layers and by patterned periodic interactions with tropomyosin over its entire length. This work serves as a structural framework for understanding the assembly and dynamics of membrane skeleton and offers insights into mechanisms of various ubiquitous F-actin-binding factors in other F-actin systems. | |||||||||
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マップデータ | ![]() | 398.8 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 28.1 KB 28.1 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 16.9 KB | 表示 | ![]() |
画像 | ![]() | 36.7 KB | ||
Filedesc metadata | ![]() | 10.3 KB | ||
その他 | ![]() ![]() | 392 MB 392 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1.1 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.1 MB | 表示 | |
XML形式データ | ![]() | 24.5 KB | 表示 | |
CIF形式データ | ![]() | 32.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8iahMC ![]() 8iaiC ![]() 8ib2C M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.37 Å | ||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_35301_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_35301_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
+全体 : Spectrin-actin junctional complex
+超分子 #1: Spectrin-actin junctional complex
+分子 #1: Adducin 1
+分子 #2: Beta-adducin
+分子 #3: Dematin actin binding protein
+分子 #4: Spectrin alpha, erythrocytic 1
+分子 #5: Actin, cytoplasmic 1
+分子 #6: Spectrin beta chain
+分子 #7: Tropomyosin-1.9
+分子 #8: Tropomyosin 3
+分子 #9: Tropomodulin-1
+分子 #10: SH3 domain-binding glutamic acid-rich-like protein
+分子 #11: ADENOSINE-5'-DIPHOSPHATE
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K2 QUANTUM (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 34.4 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.0 µm / 最小 デフォーカス(公称値): 2.0 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |